Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Cotranscriptional folding of influenza virus RNA in MDCK cells in presence of antisense oligonucleotides. A new method for next-generation sequencing based chemical mapping of RNA structure.

2017/25/B/NZ1/02269

Keywords:

RNA influenza virus therapeutic oligonucleotides PVP induced RNA mapping next-generation sequencing cotranscriptional RNA folding secondary structure of RNA

Descriptors:

  • NZ1_2: Biochemistry
  • NZ1_4: Structural biology
  • NZ1_1: Molecular biology

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

woj. wielkopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Ryszard Kierzek 

Number of co-investigators in the project: 5

Call: OPUS 13 - announced on 2017-03-15

Amount awarded: 2 068 520 PLN

Project start date (Y-m-d): 2018-01-30

Project end date (Y-m-d): 2022-01-29

Project duration:: 48 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. Aspirator próżniowy (12 000 PLN)
  2. Spektrofotometr UV/VIS z termoprogramatorem i kuwetami kwarcowymi (142 000 PLN)
  3. Aparat Real-Time PCR.
  4. Komora laminarna II klasa bezpieczeństwa (45 000 PLN)
  5. Oprogramowanie do komputera.
  6. Aparat do elektroforezy poliakrylamidowej (16 000 PLN)
  7. Inkubator z dwutlenkiem węgla (35 000 PLN)
  8. Pompa olejowa wysokiej próżni (10 000 PLN)
  9. Wysokiej sprawności komputer, monitor i drukarka laserowa (12 000 PLN)
  10. Zasilacz wysokonapięciowy (9 000 PLN)

Information in the final report

  • Publication in academic press/journals (10)
  1. Computational and NMR studies of RNA duplexes with an internal pseudouridine-adenosine base pair
    Authors:
    I. Deb, L. Popenda, J. Sarzynska, M. Malgowska, A. Lahiri, Z. Gdaniec, R. Kierzek
    Academic press:
    Scientific Reports (rok: 2019, tom: 9, strony: 16278), Wydawca: Nature Publishing Group
    Status:
    Published
    DOI:
    10.1038/s41598-019-52637-0 - link to the publication
  2. RNA Secondary Structure Motifs of the Influenza A Virus as Targets for siRNA-Mediated RNA Interference.
    Authors:
    J. Piasecka, E. Lenartowicz, M. Soszynska-Jozwiak, B. Szutkowska, R. Kierzek, E. Kierzek
    Academic press:
    Molecular Therapy-Nucleic Acids (rok: 2020, tom: 19, strony: 627-642), Wydawca: Cell Press
    Status:
    Published
    DOI:
    10.1016/j.omtn.2019.12.018 - link to the publication
  3. RNA Secondary Structure as a First Step for Rational Design of the Oligonucleotides towards Inhibition of Influenza A Virus Replication.
    Authors:
    M. Szabat, D. Lorent, T. Czapik, M. Tomaszewska, E. Kierzek, R. Kierzek
    Academic press:
    Pathogenes (rok: 2020, tom: 9, strony: 925), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/pathogens9110925 - link to the publication
  4. Secondary Structure of Influenza A Virus Genomic Segment 8 RNA Folded in a Cellular Environment
    Authors:
    B. Szutkowska, K. Wieczorek, R. Kierzek, P. Zmora, J. M. Peterson, W. N. Moss, D. H. Mathews, E. Kierzek
    Academic press:
    International Journal of Molecular Sciences (rok: 2022, tom: 23, strony: 2452), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms23052452 - link to the publication
  5. Universal and strain specific structure features of segment 8 genomic RNA of influenza A virus - application of 4-thiouridine photocrosslinking
    Authors:
    M. Soszynska-Jozwiak, M. Pszczola, J. Piasecka, J.M. Peterson, W.N. Moss, K. Taras-Goslinska, R. Kierzek, E. Kierzek
    Academic press:
    Journal of Biological Chemistry (rok: 2021, tom: 297, strony: 101245), Wydawca: ASBMB
    Status:
    Published
    DOI:
    10.1016/j.jbc.2021.101245 - link to the publication
  6. Structural variants and modifications of hammerhead ribozymes targeting influenza A virus conserved structural motifs
    Authors:
    T. Czapik, J. Piasecka, R. Kierzek, E. Kierzek
    Academic press:
    Molecular Therapy - Nucleic Acids (tom: brak, strony: brak), Wydawca: Elsevier Inc
    Status:
    Submitted
    DOI:
    brak - link to the publication
  7. Secondary structure of the segment 5 genomic RNA of influenza A virus and its application for designing antisense oligonucleotides
    Authors:
    P. Michalak, M. Soszynska-Jozwiak, E. Biala, W.N. Moss, J. Kesy, B. Szutkowska, E. Lenartowicz, R. Kierzek, E. Kierzek
    Academic press:
    Scientific Reports (rok: 2019, tom: 9, strony: 3801), Wydawca: Nuture Publishing Group
    Status:
    Published
    DOI:
    10.1038/s41598-019-40443-7 - link to the publication
  8. Conserved Structural Motifs of Two Distant IAV Subtypes in Genomic Segment 5 RNA
    Authors:
    P. Michalak, J. Piasecka, B. Szutkowska, R. Kierzek, E. Biala, W.N. Moss, E. Kierzek
    Academic press:
    VIRUSES-BASEL (rok: 2021, tom: 13, strony: 2000-2016), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/v13030525 - link to the publication
  9. The regulation properties of RNA secondary structure in alternative splicing
    Authors:
    N. Bartys, R. Kierzek, J. Lisowiec-Wachnicka
    Academic press:
    Biochimica et biophysica acta. Gene regulatory mechanisms (rok: 2019, tom: 1862, strony: 194401), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bbagrm.2019.07.002 - link to the publication
  10. Thermodynamic and structural contributions of the 6-thioguanosine residue to helical properties of RNA
    Authors:
    M. Gladysz, W. Andralojc, T. Czapik, Z. Gdaniec, R. Kierzek
    Academic press:
    Scientific Reports (rok: 2019, tom: 9, strony: 4385), Wydawca: Nature Publishing Group
    Status:
    Published
    DOI:
    10.1038/s41598-019-40715-2 - link to the publication

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