Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

Delete all

Thermodynamic insights into design of functionally optimized modified siRNAs and their experimental verification.

2013/10/E/NZ1/00741

Keywords:

siRNAs RNA thermodynamics genes silencing modified nucleotides functional chemistry and biology of RNA

Descriptors:

  • NZ1_6: Synthetic biology
  • NZ1_4: Structural biology
  • ST5_16:

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

Instytut Chemii Bioorganicznej PAN

woj. wielkopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr hab. Anna Pasternak 

Number of co-investigators in the project: 5

Call: SONATA BIS 3 - announced on 2013-06-14

Amount awarded: 810 300 PLN

Project start date (Y-m-d): 2014-07-02

Project end date (Y-m-d): 2018-04-01

Project duration:: 45 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. kołyska laboratoryjna (5 500 PLN)
  2. zasilacz do elektroforezy wysokonapięciowej (9 000 PLN)
  3. miniwirówka (5 500 PLN)
  4. elektroporator (42 000 PLN)
  5. zestaw komputerowy (komputer, monitor, drukarka) (7 000 PLN)
  6. kaseta z ekranem do fosforimigera firmy Fuji (15 000 PLN)
  7. spektrofotometr mikropłytkowy z inkubatorem.
  8. moduł grzewczy do wytrząsarki (z pokrywą) (8 000 PLN)
  9. pompa olejowa wysokiej próżni (10 000 PLN)
  10. łaźnia wodna (5 000 PLN)

Information in the final report

  • Publication in academic press/journals (8)
  1. Thermodynamic Features of Structural Motifs Formed by β-L-RNA
    Authors:
    Szabat M, Gudanis D, Kotkowiak W, Gdaniec Z, Kierzek R, Pasternak A
    Academic press:
    PLoS ONE (rok: 2016, tom: 11(2), strony: e0149478), Wydawca: Public Library of Science
    Status:
    Published
    DOI:
    10.1371/journal.pone.0149478 - link to the publication
  2. Contribution of 3′T and 3′TT overhangs to the thermodynamic stability of model siRNA duplexes
    Authors:
    Jolanta Lisowiec-Wąchnicka, Brent M. Znosko, Anna Pasternak
    Academic press:
    Biophysical Chemistry (rok: 2019, tom: 246, strony: 35-39), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bpc.2018.12.006 - link to the publication
  3. Novel isoguanine derivative of unlocked nucleic acid – investigations of thermodynamics and biological potential of modified thrombin binding aptamer
    Authors:
    Weronika Kotkowiak, Tomasz Czapik, Anna Pasternak
    Academic press:
    PLoS ONE (rok: 2018, tom: 13, strony: e0197835), Wydawca: Public Library of Science
    Status:
    Published
    DOI:
    10.1371/journal.pone.0197835 - link to the publication
  4. Improved RE31 Analogues Containing Modified Nucleic Acid Monomers: Thermodynamic, Structural, and Biological Effects
    Authors:
    Weronika Kotkowiak, Jesper Wengel, Chris J. Scotton, Anna Pasternak
    Academic press:
    Journal of Medicinal Chemistry (rok: 2019, tom: 62, strony: 2499-2507), Wydawca: ACS
    Status:
    Published
    DOI:
    10.1021/acs.jmedchem.8b01806 - link to the publication
  5. Synthesis and hybridization properties of oligonucleotide analogues with novel acyclic triazole internucleotide linkages
    Authors:
    Gladysz M., Nowak-Karnowska A., Pasternak A., Milecki J.
    Academic press:
    Bioorganic Chemistry (rok: 2017, tom: 72, strony: 161-167), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bioorg.2017.04.004 - link to the publication
  6. A systematic study on the infuence of thermodynamic asymmetry of 5′-ends of siRNA duplexes in relation to their silencing potency
    Authors:
    Jolanta Lisowiec-Wąchnicka, Natalia Bartyś, Anna Pasternak
    Academic press:
    Scientific Reports (rok: 2019, tom: 9, strony: 2477), Wydawca: Nature
    Status:
    Published
    DOI:
    10.1038/s41598-018-36620-9 - link to the publication
  7. Thermodynamic, anticoagulant, and antiproliferative properties of thrombin binding aptamer containing novel UNA derivative
    Authors:
    Kotkowiak W., Lisowiec-Wachnicka J., Grynda J., Kierzek R., Wengel J., Pasternak A.
    Academic press:
    Molecular Therapy Nucleic Acids (rok: 2018, tom: 10, strony: 304-316), Wydawca: Elsevier, Cell Press
    Status:
    Published
    DOI:
    10.1016/j.omtn.2017.12.013 - link to the publication
  8. Thermodynamic, structural and fluorescent characteristics of DNA hairpins containing functionalized pyrrolo-2'-deoxycytidines
    Authors:
    Zofia Jahnz-Wechmann, Jolanta Lisowiec-Wachnicka, Grzegorz Framski, Joanna Kosman, Jerzy Boryski, Anna Pasternak
    Academic press:
    Bioorganic Chemistry (rok: 2017, tom: 71, strony: 294-298), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bioorg.2017.02.014 - link to the publication

O Narodowym Centrum Nauki

Finansowanie nauki

Współpraca zagraniczna

Centrum prasowe

Deklaracja dostępności
Przetwarzanie danych osobowych
Polityka cookies

Partnerzy