Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

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Interdomain allosteric communication in Rad50 protein and its role in genome maintence by Mre11 complex

2016/21/B/NZ1/02847

Keywords:

Mre11 Rad50 zinc hook domain fluorescence. FRET zinc finger allostery stability

Descriptors:

  • NZ1_3: Biophysics
  • NZ1_2: Biochemistry
  • ST5_16:

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

Uniwersytet Wrocławski, Wydział Biotechnologii

woj. dolnośląskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Artur Krężel 

Number of co-investigators in the project: 7

Call: OPUS 11 - announced on 2016-03-15

Amount awarded: 1 478 920 PLN

Project start date (Y-m-d): 2017-01-12

Project end date (Y-m-d): 2020-11-11

Project duration:: 46 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. 2 zestawy komputerowe do analizy danych i obliczeń.
  2. Spektrofluorymetr do pomiaru czasu zaniku fluorescencji (350 000 PLN)

Information in the final report

  • Publication in academic press/journals (15)
  • Articles in post-conference publications (26)
  1. Fluorescent probes for selective protein labeling in lysosomes: a case of alpha-galactosidase A
    Authors:
    Cornelius Bohl, Adam Pomorski, Susanne Seemann, Anne-Marie Knospe, Chaonan Zheng, Artur Krężel, Arndt Rolfs, Jan Lukas
    Academic press:
    The FASEB Journal (rok: 2017, tom: 31, strony: 5258-5267), Wydawca: Federation of American Societies for Experimental Biology
    Status:
    Published
    DOI:
    10.1096/fj.201700058RRRR - link to the publication
  2. Zeptomolar Ag(I) affinity to Rad50 zinc hook domain leads to interprotein complex disruption through the formation of Ag4S4 cluster
    Authors:
    Olga Kerber, Józef Tran, Alicja Misiaszek, Aleksandra Chorążewska, Wojciech Bal, Artur Krężel
    Academic press:
    Inorganic Chemistry , Wydawca: American Chemical Society
    Status:
    Submitted
    DOI:
    brak - link to the publication
  3. Formation of highly stable multinuclear AgnSn clusters in zinc fingers disrupts their structure and function
    Authors:
    Katarzyna Kluska, Manuel D. Peris-Dıiaz, Dawid Płonka, Alexander Moysa, Michał Dadlez, Aurelien Deniaud, Wojciech Bal, Artur Krężel
    Academic press:
    Chemical Communications (rok: 2020, tom: 56, strony: 1329--1332), Wydawca: Royal Chemical Society
    Status:
    Published
    DOI:
    10.1039/c9cc09418k - link to the publication
  4. Reactivity of Cu(II)-, Zn(II)- and Fe(II)-Thiosemicarbazone Complexes with Glutathione and Metallothionein: from Stability over Dissociation to Transmetallation
    Authors:
    Alice Santoro, Bertrand Vileno, Òscar Palacios, Manuel David Peris-Díaz, Gilles Riegel, Christian Gaiddon, Artur Krężel, Peter Faller
    Academic press:
    Metallomics (rok: 2019, tom: 11, strony: 994-1004), Wydawca: Royal Chemical Society
    Status:
    Published
    DOI:
    10.1039/C9MT00061E - link to the publication
  5. Chemometrics-assisted optimization of liquid chromatography-quadrupole-time-of-flight mass spectrometry analysis for targeted metabolomics
    Authors:
    Manuel David Peris-Díaz, Olga Rodak, Shannon R. Sweeney, Artur Krężel, Enrique Sentandreu
    Academic press:
    Talanta (rok: 2019, tom: 199, strony: 380-387), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.talanta.2019.02.075 - link to the publication
  6. Mass Spectrometry-Based Structural Analysis of Cysteine-Rich Metal-Binding Sites in Proteins with MetaOdysseus R Software
    Authors:
    Manuel David Peris-Diaz
    Academic press:
    Journal of Proteome Reserach (rok: 2021, tom: 20, strony: 776-785), Wydawca: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/acs.jproteome.0c00651 - link to the publication
  7. Metal Exchange in the Interprotein ZnII-Binding Site of the Rad50 Hook Domain: Structural Insights into CdII-Induced DNA-Repair Inhibition
    Authors:
    Michał Padjasek, Maciej Maciejczyk, Michał Nowakowski, Olga Kerber, Maciej Pyrka, Wiktor Koźmiński, Artur Krężel
    Academic press:
    Chemistry - A European Journal (rok: 2020, tom: 26, strony: 3297 – 3313), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/chem.201904942 - link to the publication
  8. Relations between Structure and Zn(II) Binding Affinity Shed Light on the Mechanisms of Rad50 Hook Domain Functioning and Its Phosphorylation
    Authors:
    Józef B. Tran, Michał Padjasek, Artur Krężel
    Academic press:
    International Journal of Molecular Sciences (rok: 2022, tom: 23, strony: 11140), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms231911140 - link to the publication
  9. Galvanization of Protein–Protein Interactions in a Dynamic Zinc Interactome
    Authors:
    Anna Kocyła, Józef Tran, Artur Krężel
    Academic press:
    Trends in Biochem Sciences (rok: 2021, tom: 46, strony: 64-79), Wydawca: Cell Press / Elsevier
    Status:
    Published
    DOI:
    10.1016/j.tibs.2020.08.011 - link to the publication
  10. InterMetalDB: A Database and Browser of Intermolecular Metal Binding Sites in Macromolecules with Structural Information
    Authors:
    Józef Ba Tran, Artur Krężel
    Academic press:
    Journal of Proteome Research (rok: 2021, tom: 20, strony: 1889-1901), Wydawca: American Chemical Society (ACS)
    Status:
    Published
    DOI:
    10.1021/acs.jproteome.0c00906 - link to the publication
  11. Molar absorption coefficients and stability constants of Zincon metal complexes for determination of metal ions and bioinorganic applications
    Authors:
    Anna Kocyła, Adam Pomorski, Artur Krężel
    Academic press:
    Journal of Inorganic Biochemistry (rok: 2017, tom: 176, strony: 53-65), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.jinorgbio.2017.08.006 - link to the publication
  12. Optimized allosteric inhibition of engineered protein tyrosine phosphatases with an expanded palette of biarsenical small molecules
    Authors:
    Samuel Korntner, Adam Pomorski, Artur Krężel, Anthony C. Bishop
    Academic press:
    Bioorganic and Medicinal Chemistry (rok: 2018, tom: 26, strony: 2610-2620), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bmc.2018.04.026 - link to the publication
  13. An Extremely Stable Interprotein Tetrahedral Hg(Cys)4 Core Formed in the Zinc Hook Domain of Rad50 Protein at Physiological pH
    Authors:
    Marek Łuczkowski, Michał Padjasek, Józef B. Tran, Lars Hemmingsen, Olga Kerber, Jelena Habjanič, Eva Freisinger, Artur Krężel
    Academic press:
    Chemistry - A European Journal , Wydawca: Wiley
    Status:
    Accepted for publication
    DOI:
    10.1002/chem.202202738 - link to the publication
  14. Phytochelatins as a Dynamic System for Cd(II) Buffering from the Micro- to Femtomolar Range
    Authors:
    Joanna Wątły, Marek Łuczkowski, Michał Padjasek, Artur Krężel
    Academic press:
    Inorganic Chemistry (rok: 2021, tom: 60, strony: 4657-4675), Wydawca: American Chemical Society (ACS)
    Status:
    Published
    DOI:
    10.1021/acs.inorgchem.0c03639 - link to the publication
  15. Structural zinc binding sites shaped for greater works: Structure-function relations in classical zinc finger, hook and clasp domains
    Authors:
    Michał Padjasek, Anna Kocyła, Katarzyna Kluska, Olga Kerber, Józef Ba Tran, Artur Krężel
    Academic press:
    Journal of Inorganic Biochemistry (rok: 2020, tom: 204, strony: 110955), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.jinorgbio.2019.110955 - link to the publication
  1. Deciphering Cd(II) ion's impact on intermolecular Zn(II)-binding site of Rad50 zinc hook domain
    Authors:
    Michał Padjasek, Artur Krężel
    Conference:
    25-th Polish Peptide Symosium (rok: 2019, ), Wydawca: Uniwersytet Medyczny we Wrocławiu
    Data:
    konferencja 2019-09-08 - 2019-08-12
    Status:
    Published
  2. Insights into the origin of weakly bound Zn(II) ion in metallothionein proteins from computational modeling
    Authors:
    Manuel David Peris-Díaz, Artur Krężel
    Conference:
    7-th International Symposium on Metallomics (rok: 2019, ), Wydawca: Politechnika Warszawska
    Data:
    konferencja 2019-06-30 - 2019-07-03
    Status:
    Published
  3. Investigation of zinc hook domain of Rad50 from S. cerevisiae - spectroscopic stability studies of minimal and full domain length peptides interactions with Zn(II)
    Authors:
    Józef Tran, Michał Padjasek, Artur Krężel
    Conference:
    24-th Polish Peptide Symposium (rok: 2017, ), Wydawca: Uniwersytet Gdański
    Data:
    konferencja 3-7.09
    Status:
    Published
  4. Metal dependent folding of a naturally altered zinc finger domains – insight into coordination mode, stability and function
    Authors:
    Katarzyna Kluska, Artur Krężel
    Conference:
    7-th International Symposium on Metallomics (rok: 2019, ), Wydawca: Politechnika Warszawska
    Data:
    konferencja 2019-06-30 - 2019-07-03
    Status:
    Published
  5. Phosphorylation in zinc hook domain of Rad50 protein - stability and architecture Investigation
    Authors:
    Józef Tran, Michał Padjasek, Artur Krężel
    Conference:
    7-th International Symposium on Metallomics (rok: 2019, ), Wydawca: Politechnika Warszawska
    Data:
    konferencja 2019-06-30 - 2019-07-03
    Status:
    Published
  6. Relationship of the structural and zinc buffering properties of human metallothioneins
    Authors:
    Artur Krężel, Manuel David Peris-Díaz, Agnieszka Drozd, Dominika Wojewska, Roman Guran, Adam Pomorski, Ondrej Zitka, Vojtech Adam
    Conference:
    7-th International Symposium on Metallomics (rok: 2019, ), Wydawca: Politechnika Warszawska
    Data:
    konferencja 2019-06-30 - 2019-07-03
    Status:
    Published
  7. Składanie białek z wykorzystaniem peptydowych motywow dimeryzujących zależnych od jonow Zn(II)
    Authors:
    Anna Kocyła, Artur Krężel
    Conference:
    62 Zjazd PTChem Warszawa (rok: 2019, ), Wydawca: Politechnika Warszawska
    Data:
    konferencja 2019-09-02 - 2019-09.06
    Status:
    Published
  8. Zinc Hook Domain – Puzzling Rad50 Interprotein Zn(II) Binding Site with Many Faces
    Authors:
    Józef Tran, Olga Kerber, Michał Padjasek, Maciej Maciejczyk, Marek Łuczkowski, Tomasz Kochańczyk, Artur Krężel
    Conference:
    19th International Conference on Biological Inorganic Chemistry (rok: 2019, ), Wydawca: University of Zurich
    Data:
    konferencja 2019-08-11 - 2019-08-16
    Status:
    Published
  9. Biophysical characterization of Rad50 zinc hook domain from thermophilic archeon Thermoplasma acidophilum
    Authors:
    Olga Kerber, Artur Krężel
    Conference:
    43-rd FEBS Congress (rok: 2018, ), Wydawca: FEBS
    Data:
    konferencja 7-12.07
    Status:
    Published
  10. Bisarsenowe sondy fluorescencyjne - Chemia na pomoc biologii
    Authors:
    Adam Pomorski
    Conference:
    60 Zjazd Naukowy Polskiego Towarzystwa Chemicznego (rok: 2017, ), Wydawca: Politechnika Wrocławska
    Data:
    konferencja 17-21.09
    Status:
    Published
  11. How proteins match Zn(II) affinity with their functions? Relations between thermodynamics and free zinc status
    Authors:
    Adam Pomorski, Agnieszka drozd, Katarzyna Kluska, Justyna Adamczyk, Anna Kocyła, Artur Krężel
    Conference:
    International Society for Zinc in Biology ISZB 5-th meeting (rok: 2017, ), Wydawca: UCLAN
    Data:
    konferencja 18-22.06
    Status:
    Published
  12. Insight into the cellular buffering mechanism through structural characterization of partially-depleted methallothionein 2
    Authors:
    Manuel David Peris-Díaz, Dominika Wojewska, Agnieszka Drozd, Artur Krężel
    Conference:
    11-th International Biometals Symposium (rok: 2018, ), Wydawca: University of Ottawa
    Data:
    konferencja 15-19.07
    Status:
    Published
  13. Structural and energetic factors that control zinc protein domains stability and function
    Authors:
    Justyna Adamczyk, Anna Kocyła, Michał Padjasek, Katarzyna Kluska, Artur Krężel
    Conference:
    24-th Polish Peptide Symposium (rok: 2017, ), Wydawca: Uniwersytet Gdański
    Data:
    konferencja 3-7.09
    Status:
    Published
  14. Biophysical characterization of Rad50 zinc hook domain from various thermophilic organisms
    Authors:
    Olga Kerber, Katarzyna Kluska, Artur Krężel
    Conference:
    7-th International Symposium on Metallomics (rok: 2019, ), Wydawca: Politechnika Warszawska
    Data:
    konferencja 2019-06-30 - 2019-07-03
    Status:
    Published
  15. Relationship between the architecture of zinc coordination and zinc binding affinity in proteins-insights into zinc regulation
    Authors:
    Katarzyna Kluska, Olga Kerber, Adam Pomorski, Anna Kocyła, Michał Padjasek, Józef Tran, Manuel Peris-Diáz, Justyna Adamczyk, Artur Krężel
    Conference:
    61 Zjazd Naukowy Polskiego Towarzystwa Chemicznego (rok: 2018, ), Wydawca: Uniwersytet Jagielloński
    Data:
    konferencja 17-21.09
    Status:
    Published
  16. Wpływ sposobu koordynacji oraz powinowactwa jonów Zn(II) na komórkową funkcję metalobiałek
    Authors:
    Katarzyna Kluska, Anna Kocyła, Olga Kerber, Józef Tran, Michał Padjasek, Manuel Peris-Díaz, Artur Krężel
    Conference:
    62 Zjazd PTChem Warszawa (rok: 2019, ), Wydawca: Politechnika Warszawska
    Data:
    konferencja 2019-09-02 - 2019-09-06
    Status:
    Published
  17. Biophysical properties of Rad50 zinc hook domain
    Authors:
    Olga Kerber, Artur Krężel
    Conference:
    25-th Polish Peptide Symosium (rok: 2019, ), Wydawca: Uniwersytet Medyczny we Wrocławiu
    Data:
    konferencja 2019-09-08 - 2019-09-12
    Status:
    Published
  18. Effect of silver ions on zinc fingers structure and function – spectroscopic and stability studies
    Authors:
    Katarzyna Kluska, Dawid Płonka, Olga Kerber, Wojciech Bal, Artur Krężel
    Conference:
    7-th International Symposium on Metallomics (rok: 2019, ), Wydawca: Politechnika Warszawska
    Data:
    konferencja 2019-06-30 - 2019-07-03
    Status:
    Published
  19. Insight into Rad50's phosphorylation – stability and Zn(II) binding
    Authors:
    Józef Tran, Michał Padjasek, Artur Krężel
    Conference:
    25-th Polish Peptide Symosium (rok: 2019, ), Wydawca: Uniwersytet Medyczny we Wrocławiu
    Data:
    konferencja 2019-09-08 - 2019-09-12
    Status:
    Published
  20. Metal-binding properties of the eukaryotic Rad50 hook domain variants – insights into the sequence-stability relationship
    Authors:
    Olga Kerber, Artur Krężel
    Conference:
    16-th European Biological Inorganic Chemistry Conference (rok: 2022, ), Wydawca: EuroBIC-16
    Data:
    konferencja 17-21.07
    Status:
    Published
  21. Metal-coupled folding as the driving force for zinc proteins function - insight into sequence-structure-stability relationships in zinc finger, hook and clasp domains
    Authors:
    Olga Kerber, Michał Padjasek, Józef B. Tran, Katarzyna Kluska, Anna Kocyła, Artur Krężel
    Conference:
    17-th Naples Workshop on Bioactive Peptides (rok: 2022, ), Wydawca: 17-th Naples Workshop on Bioactive Peptides
    Data:
    konferencja 16-18.06
    Status:
    Published
  22. Regulacja i deregulacja funkcji białek cynkowych - Walka dobrego z dobrym i złym
    Authors:
    Artur Krężel
    Conference:
    64 Zjazd Naukowy PTChem (rok: 2022, ), Wydawca: UMCS
    Data:
    konferencja 11-16.09
    Status:
    Published
  23. Sequence-structure-stability relations in zinc hook, zinc clasp and altered zinc finger protein domains
    Authors:
    Katarzyna Kluska, Olga Kerber, Anna Kocyła, Józef Tran, Michał Padjasek, Artur Krężel
    Conference:
    International Symposium on Metal Complexes (rok: 2019, ), Wydawca: University of Debrecen
    Data:
    konferencja 2019-06-11 - 2019-06-14
    Status:
    Published
  24. Beyond classical binding sites in zinc proteins - biophysical overview of zinc hook, zinc clasp and altred zinc fingers
    Authors:
    Anna Kocyła, Michał Padjasek, Katarzyna Kluska, Justyna Adamczyk, Olga Kerber, Józef Tran, Artur Krężel
    Conference:
    Eurobic 14 (rok: 2018, ), Wydawca: University of Birmingham
    Data:
    konferencja 26-30.08
    Status:
    Published
  25. How proteins match Zn(II) affinity to fit their function? Relationship between thermodynamics and free zinc status
    Authors:
    Michał Padjasek, Anna Kocyła, Katarzyna Kluska, Justyna Adamczyk, Manuel David Peris Díaz, Artur Krężel
    Conference:
    11-th International Biometals Symposium (rok: 2018, ), Wydawca: University of Ottawa
    Data:
    konferencja 15-19.07
    Status:
    Published
  26. Relationships of the structural and zinc buffering properties of human metallothioneins
    Authors:
    Manuel D. Peris-Diaz, Agnieszka Drozd, Dominika Wojewska, Roman Guran, Adam Pomorski, Ondriej Zitka, Vojtech Adam, Artur Krężel
    Conference:
    The 6-th Meeting of International Society for Zinc Biology (rok: 2019, ), Wydawca: Kyoto University
    Data:
    konferencja 2019-09-06 - 2019-09-13
    Status:
    Published

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