Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

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Pharmacotherapeutic mechanisms, spectroscopic signatures, and nanomechanics of dysfunctional endothelium in liver steatosis and heart failure; towards organ-specyfic mechanisms

2015/16/W/NZ4/00070

Keywords:

endothelium liver heart dysfunction

Descriptors:

  • NZ4_4: Pathophysiology
  • NZ7_14: Pharmacy, pharmacotherapy, pharmacology
  • NZ1_3: Biophysics

Panel:

NZ4 - Biology of tissues, organs and organisms: morphology and functions of animal's and human's systems, organs and organisms, experimental medicine, basics of neurology

Host institution :

Uniwersytet Jagielloński, Jagiellońskie Centrum Rozwoju Leków (JCET), Pracownia Farmakologii Doświadczalnej Śródbłonka

woj. małopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Stefan Chłopicki 

Number of co-investigators in the project: 15

Call: SYMFONIA 3

Amount awarded: 6 996 680 PLN

Project start date (Y-m-d): 2015-12-02

Project end date (Y-m-d): 2021-04-01

Project duration:: 64 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. System do kontroli i akwizycji danych skaningowego mikroskopu próbkującego umożliwiajacy integrację ze spektrometrem ramanowskim (200 000 PLN)
  2. Głowice lasera do cytometru obrazowego CQ1: 405nm oraz 640nm wraz z zestawem filtrów emisyjnych..

Information in the final report

  • Publication in academic press/journals (31)
  1. AFM image analysis of porous structures by means of neural networks
    Authors:
    Giergiel M., Zapotoczny B., Czyzynska-Cichon I., Konior J., Szymonski M.
    Academic press:
    Biomedical Signal Processing and Control (rok: 2021, tom: 71, strony: nd.), Wydawca: Elsevier Ltd.
    Status:
    Published
    DOI:
    10.1016/j.bspc.2021.103097 - link to the publication
  2. Application of a layered model for determination of the elasticity of biological systems
    Authors:
    Rusaczonek M, Zapotoczny B, Szymonski M, Konior J
    Academic press:
    Micron (rok: 2019, tom: 124, strony: nd.), Wydawca: Elsevier Ltd.
    Status:
    Published
    DOI:
    10.1016/j.micron.2019.102705 - link to the publication
  3. Enhanced cardiac hypoxic injury in atherogenic dyslipidaemia results from alterations in the energy metabolism pattern
    Authors:
    Olkowicz M, Tomczyk M, Debski J, Tyrankiewicz U, Przyborowski K, Borkowski T, Zabielska-Kaczorowska M, Szupryczynska N, Kochan Z, Smeda M, Dadlez M, Chlopicki S, Smolenski RT
    Academic press:
    Metabolism Clinical and Experimental (rok: 2021, tom: 114, strony: nd.), Wydawca: Elsevier Inc.
    Status:
    Published
    DOI:
    10.1016/j.metabol.2020.154400 - link to the publication
  4. MRI-based assessment of liver perfusion and hepatocyte injury in the murine model of acute hepatitis
    Authors:
    Byk K, Jasinski K, Bartel Z, Jasztal A, Sitek B, Tomanek B, Chlopicki S, Skorka T
    Academic press:
    Magn Reson Mater Phys Biol Med (rok: 2016, tom: 29, strony: 789-798), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s10334-016-0563-2 - link to the publication
  5. Morphology and force probing of primary murine liver sinusoidal endothelial cells
    Authors:
    Zapotoczny B, Owczarczyk K, Szafranska K, Kus E, Chlopicki S, Szymonski M
    Academic press:
    Journal of Molecular Recognition (rok: 2017, tom: 30, strony: 45299), Wydawca: John Wiley & Sons Ltd.
    Status:
    Published
    DOI:
    10.1002/jmr.2610 - link to the publication
  6. Multiorgan Development of Oxidative and Nitrosative Stress in LPS-Induced Endotoxemia in C57Bl/6 Mice: DHE-Based In Vivo Approach
    Authors:
    Proniewski B, Kij A, Sitek B, Kelley EE, Chlopicki S
    Academic press:
    Oxidative Medicine and Cellular Longevity (rok: 2019, tom: 2019, strony: nd.), Wydawca: Hindawi
    Status:
    Published
    DOI:
    10.1155/2019/7838406 - link to the publication
  7. Raman imaging-based phenotyping of murine primary endothelial cells to identify disease-associated biochemical alterations
    Authors:
    Tott S., Grosicki M., Glowacz J., Mohaissen T., Wojnar-Lason K., Chlopicki S., Baranska M.
    Academic press:
    Biochimica et Biophysica Acta (BBA)- Molecular Basis of Disease (rok: 2021, tom: 1867, strony: nd.), Wydawca: Elsevier B.V.
    Status:
    Published
    DOI:
    10.1016/j.bbadis.2021.166180 - link to the publication
  8. Raman spectroscopic features of primary cardiac microvascular endothelial cells(CMECs) isolated from the murine heart
    Authors:
    S. Tott, M. Grosicki, B. Klimas, D. Augustynska, S. Chlopicki, M. Baranska
    Academic press:
    Analyst (rok: 2018, tom: 143, strony: 6079–6086), Wydawca: ROYAL SOCIETY OF CHEMISTRY
    Status:
    Published
    DOI:
    10.1039/c8an01308j - link to the publication
  9. Vascular Cognitive Impairment Linked to Brain Endothelium Inflammation in Early Stages of Heart Failure in Mice
    Authors:
    M.G. Adamski, M. Sternak, T. Mohaissen, D. Kaczor, J.M. Wierońska, M. Malinowska, I. Czaban, K. Byk, K. S. Lyngsø, K. Przyborowski, Pernille B.L. Hansen, G. Wilczyński, S. Chłopicki,
    Academic press:
    Journal of the American Heart Association (rok: 2018, tom: 7, strony: nd.), Wydawca: American Heart Association
    Status:
    Published
    DOI:
    10.1161/JAHA.117.007694 - link to the publication
  10. Activation pattern of ACE2/Ang-(1–7) and ACE/Ang II pathway in course of heart failure assessed by multiparametric MRI in vivo in Tgq*44 mice
    Authors:
    U. Tyrankiewicz, M. Olkowicz, T. Skórka, M. Jabłońska, A. Orzyłowska, A. Bar, M. Gonet, P. Berkowicz, K. Jasiński, J. A. Żołądź, R. T. Smoleński, S. Chłopicki
    Academic press:
    JJournal of Applied Physiology (rok: 2018, tom: 124, strony: 52 - 65), Wydawca: American Physiological Society
    Status:
    Published
    DOI:
    10.1152/japplphysiol.00571.2017 - link to the publication
  11. Atomic Force Microscopy Reveals the Dynamic Morphology of Fenestrations in Live Liver Sinusoidal Endothelial Cells
    Authors:
    Zapotoczny B, Szafranska K, Owczarczyk K, Kus E, Chlopicki S, Szymonski M.
    Academic press:
    Scientific Reports (rok: 2017, tom: 7, strony: nd), Wydawca: Nature Publishing Group
    Status:
    Published
    DOI:
    10.1038/s41598-017-08555-0 - link to the publication
  12. Differential response of liver sinusoidal endothelial cells and hepatocytes to oleic and palmitic acid revealed by Raman and CARS imaging
    Authors:
    Matuszyk E., Sierka E., Bae H., Meyer T., Kus E., Chlopicki S., Schmitt M., Popp J., Baranska M.
    Academic press:
    Biochimica et Biophysica Acta (BBA)- Molecular Basis of Disease (rok: 2020, tom: 1866, strony: -), Wydawca: Elsevier B.V.
    Status:
    Published
    DOI:
    10.1016/j.bbadis.2020.165763 - link to the publication
  13. Immuno-Spin Trapping-Based Detection of Oxidative Modifications in Cardiomyocytes and Coronary Endothelium in the Progression of Heart Failure in Tgαq*44 Mice
    Authors:
    Proniewski B., Czarny J., Khomich T., Kus K., Zakrzewska A., Chlopicki S.
    Academic press:
    Frontiers in Immunology (rok: 2018, tom: 9, strony: nd.), Wydawca: Frontiers
    Status:
    Published
    DOI:
    10.3389/fimmu.2018.00938 - link to the publication
  14. Label-free spectroscopic characterization of live liver sinusoidal endothelial cells (LSECs) isolated from the murine liver
    Authors:
    Kochan K, Kus E, Filipek A, Szafranka K, Chlopicki S, Baranska M
    Academic press:
    Analyst (rok: 2017, tom: 142, strony: 1308-1319), Wydawca: ROYAL SOCIETY OF CHEMISTRY
    Status:
    Published
    DOI:
    10.1039/c6an02063a - link to the publication
  15. Actin-spectrin scaffold supports open fenestrae in liver sinusoidal endothelial cells
    Authors:
    Zapotoczny B, Braet F, Kus E, Ginda- Mäkelä K, Klejevskaja B, Campagna R, Chlopicki S, Szymonski M
    Academic press:
    Traffic (rok: 2019, tom: 20, strony: 932-942), Wydawca: John Wiley & Sons Ltd.
    Status:
    Published
    DOI:
    10.1111/tra.12700 - link to the publication
  16. Biophysical nanocharacterization of liver sinusoidal endothelial cells through atomic force microscopy
    Authors:
    Zapotoczny B., Braet F., Wisse E., Lekka M., Szymonski M.
    Academic press:
    Biophysical Reviews (rok: 2020, tom: 12, strony: 625–636), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s12551-020-00699-0 - link to the publication
  17. Quantitative analysis methods for studying fenestrations in liver sinusoidal endothelial cells
    Authors:
    Szafranska K., Holte CF., Kruse LD., Mao H., Øie CI., Szymonski M., Zapotoczny B., McCourt AG.
    Academic press:
    Micron (rok: 2021, tom: 150, strony: nd.), Wydawca: Elsevier Ltd.
    Status:
    Published
    DOI:
    10.1016/j.micron.2021.103121 - link to the publication
  18. Short-term treatment with hepatoselective NO donor V-PYRRO/NO improves blood flow in hepatic microcirculation in liver steatosis in mice
    Authors:
    Kuś E, Jasiński K, Skórka T, Czyżyńska-Cichoń I, Chłopicki S.
    Academic press:
    Pharmacological Reports (rok: 2018, tom: 70, strony: 463 - 469), Wydawca: Elsevier Sp. z o.o.
    Status:
    Published
    DOI:
    10.1016/j.pharep.2017.11.019 - link to the publication
  19. Tracking Fenestrae Dynamics in Live Murine Liver Sinusoidal Endothelial Cells
    Authors:
    B. Zapotoczny, K. Szafranska, E. Kus, F. Braet, E. Wisse, S. Chlopicki, M. Szymonski
    Academic press:
    Hepatology (rok: 2018, tom: 69, strony: 876 - 888), Wydawca: American Association for the Study of Liver Diseases
    Status:
    Published
    DOI:
    10.1002/hep.30232 - link to the publication
  20. Voluntary physical activity counteracts Chronic Heart Failure progression affecting both cardiac function and skeletal muscle in the transgenic Tgaq*44 mouse model
    Authors:
    Bardi E, Majerczak J, Zoladz JA, Tyrankiewicz U, Skorka T , Chlopicki S, Jablonska M, Bar A, Jasinski K, Buso A, Salvadego D, Nieckarz Z, Grassi B, Bottinelli R, Pellegrino MA
    Academic press:
    Physiological Reports (rok: 2019, tom: 7, strony: nd.), Wydawca: Wiley Periodicals Inc.
    Status:
    Published
    DOI:
    10.14814/phy2.14161 - link to the publication
  21. Eicosanoid profiling in effluent of isolated perfused heart of Tgαq*44 mice with advanced heart failure.
    Authors:
    Berkowicz P, Kij A, Walczak M, Chlopicki S
    Academic press:
    Journal of Physiology and Pharmacology (rok: 2019, tom: 70, strony: 135-142), Wydawca: Polskie Towarzystwo Fizjologiczne
    Status:
    Published
    DOI:
    10.26402/jpp.2019.1.13 - link to the publication
  22. Functional and Biochemical Endothelial Profiling In Vivo in a Murine Model of Endothelial Dysfunction; Comparison of Effects of 1-Methylnicotinamide and Angiotensin-converting Enzyme Inhibitor
    Authors:
    Bar A, Olkowicz M, Tyrankiewicz U, Kus E, Jasinski,K, Smolenski RT, Skorka T, Chlopicki S
    Academic press:
    Frontiers in Pharmacology (rok: 2017, tom: 8, strony: nd), Wydawca: Frontiers
    Status:
    Published
    DOI:
    10.3389/fphar.2017.00183 - link to the publication
  23. Physical Activity and Inhibition of ACE Additively Modulate ACE/ACE-2 Balance in Heart Failure in Mice
    Authors:
    Tyrankiewicz U., Olkowicz M., Berkowicz P., Jablonska M., Smolenski R.T., Zoladz J.A. and Chlopicki S.
    Academic press:
    Frontiers in Pharmacology (rok: 2021, tom: 12, strony: nd.), Wydawca: Frontiers Media SA
    Status:
    Published
    DOI:
    10.3389/fphar.2021.682432 - link to the publication
  24. Quantification of fenestrations in liver sinusoidal endothelial cells by atomic force microscopy
    Authors:
    Zapotoczny B, Szafranska K, Kus E, Chlopicki S, Szymonski M.
    Academic press:
    Micron (rok: 2017, tom: 101, strony: 48–53), Wydawca: Elsevier Ltd.
    Status:
    Published
    DOI:
    10.1016/j.micron.2017.06.005 - link to the publication
  25. Retrospectively gated MRI for in vivo assessment of endothelium-dependent vasodilatation and endothelial permeability in murine models of endothelial dysfunction
    Authors:
    Bar A, Skórka T, Jasiński K, Sternak M, Bartel Z, Tyrankiewicz U, Chlopicki S
    Academic press:
    NMR Biomed (rok: 2016, tom: 29, strony: 1088-1097), Wydawca: John Wiley & Sons, Ltd.
    Status:
    Published
    DOI:
    10.1002/nbm.3567 - link to the publication
  26. Changes induced by non-alcoholic fatty liver disease in liver sinusoidal endothelial cells and hepatocytes: spectroscopic imaging of single live cells at the subcellular level
    Authors:
    K. Kochan, E. Kus, E. Szafraniec, A. Wislocka, S. Chlopicki, M. Baranska
    Academic press:
    Analyst (rok: 2017, tom: 142, strony: 3948-3958), Wydawca: ROYAL SOCIETY OF CHEMISTRY
    Status:
    Published
    DOI:
    10.1039/c7an00865a - link to the publication
  27. Combined Raman- and AFM-based detection of biochemical and nanomechanical features of endothelial dysfunction in aorta isolated from ApoE/LDLR-/- mice.
    Authors:
    Maase M, Rygula A, Pacia MZ, Proniewski B, Mateuszuk L, Sternak M, Kaczor A, Chlopicki S, Kusche-Vihrog K.
    Academic press:
    Nanomedicine: Nanotechnology, Biology, and Medicine (rok: 2019, tom: 16, strony: 97-105), Wydawca: Elsevier Inc.
    Status:
    Published
    DOI:
    10.1016/j.nano.2018.11.014 - link to the publication
  28. Degradation of Glycocalyx and Multiple Manifestations of Endothelial Dysfunction Coincide in the Early Phase of Endothelial Dysfunction Before Atherosclerotic Plaque Development in Apolipoprotein E/Low-Density Lipoprotein Receptor-Deficient Mice
    Authors:
    Bar A, Targosz- Korecka M, Suraj J, Proniewski B, Jasztal A, Marczyk B, Sternak M, Przybyło M, Kurpinska A, Walczak M, Kostogrys R.B, Szymonski M, Chlopicki S.
    Academic press:
    Journal of the American Heart Association (rok: 2019, tom: 8, strony: nd.), Wydawca: American Heart Association
    Status:
    Published
    DOI:
    10. - link to the publication
  29. LSEC Fenestrae Are Preserved Despite Pro-inflammatory Phenotype of Liver Sinusoidal Endothelial Cells in Mice on High Fat Diet
    Authors:
    Kus E, Kaczara P, Czyzynska-Cichon I, Szafranska K, Zapotoczny B, Kij A, Sowinska A, Kotlinowski J, Mateuszuk L, Czarnowska E, Szymonski M, Chlopicki S
    Academic press:
    Frontiers in Physiology (rok: 2019, tom: 10, strony: nd.), Wydawca: Frontiers
    Status:
    Published
    DOI:
    10.3389/fphys.2019.00006 - link to the publication
  30. Raman spectroscopy–based insight into lipid droplets presence and contents in liver sinusoidal endothelial cells and hepatocytes
    Authors:
    E. Szafraniec, E. Kus, A. Wislocka, B. Kukla, E. Sierka, V. Untereiner, G.D. Sockalingum, S. Chlopicki, M. Baranska
    Academic press:
    Journal of Biophotonics (rok: 2018, tom: e201800290, strony: 45302), Wydawca: WILEY-VCH Verlag GmbH & Co.
    Status:
    Published
    DOI:
    10.1002/jbio.201800290 - link to the publication
  31. Vibrational spectroscopy-based quantification of liver steatosis
    Authors:
    Szafraniec E, Tott S, Kus E, Augustynska D, Jasztal A, Filipek A, Chlopicki S, Baranska M
    Academic press:
    Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease (rok: 2019, tom: 1865, strony: nd.), Wydawca: Elsevier B.V.
    Status:
    Published
    DOI:
    10.1016/j.bbadis.2019.08.002 - link to the publication

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