Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

Delete all

Pharmacotherapeutic mechanisms, spectroscopic signatures, and nanomechanics of dysfunctional endothelium in liver steatosis and heart failure; towards organ-specyfic mechanisms

2015/16/W/NZ4/00070

Keywords:

endothelium liver heart dysfunction

Descriptors:

  • NZ4_4:
  • NZ7_14:
  • NZ1_3:

Panel:

NZ4 - Biology of tissues, organs and organisms: morphology and functions of animal's and human's systems, organs and organisms, experimental medicine, basics of neurology

Host institution :

Uniwersytet Jagielloński, Jagiellońskie Centrum Rozwoju Leków (JCET); Pracownia Farmakologii Doświadczalnej Śródbłonka

woj. małopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Stefan Karol Chłopicki 

Number of co-investigators in the project: 15

Call: SYMFONIA 3 - announced on 2014-12-15

Amount awarded: 6 450 560 PLN

Project start date (Y-m-d): 2015-12-02

Project end date (Y-m-d): 2021-04-01

Project duration:: 64 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Głowice lasera do cytometru obrazowego CQ1: 405nm oraz 640nm wraz z zestawem filtrów emisyjnych..
  2. System do kontroli i akwizycji danych skaningowego mikroskopu próbkującego umożliwiajacy integrację ze spektrometrem ramanowskim (200 000 PLN)

Information in the final report

  • Publication in academic press/journals (36)
  1. Actin-spectrin scaffold supports open fenestrae in liver sinusoidal endothelial cells
    Authors:
    Zapotoczny B, Braet F, Kus E, Ginda- Mäkelä K, Klejevskaja B, Campagna R, Chlopicki S, Szymonski M
    Academic press:
    Traffic (rok: 2019, tom: 20, strony: 932-942), Wydawca: John Wiley & Sons Ltd.
    Status:
    Published
    DOI:
    10.1111/tra.12700 - link to the publication
  2. Voluntary physical activity counteracts Chronic Heart Failure progression affecting both cardiac function and skeletal muscle in the transgenic Tgaq*44 mouse model
    Authors:
    Bardi E, Majerczak J, Zoladz JA, Tyrankiewicz U, Skorka T , Chlopicki S, Jablonska M, Bar A, Jasinski K, Buso A, Salvadego D, Nieckarz Z, Grassi B, Bottinelli R, Pellegrino MA
    Academic press:
    Physiological Reports (rok: 2019, tom: 7, strony: nd.), Wydawca: Wiley Periodicals Inc.
    Status:
    Published
    DOI:
    10.14814/phy2.14161 - link to the publication
  3. Vascular Cognitive Impairment Linked to Brain Endothelium Inflammation in Early Stages of Heart Failure in Mice
    Authors:
    M.G. Adamski, M. Sternak, T. Mohaissen, D. Kaczor, J.M. Wierońska, M. Malinowska, I. Czaban, K. Byk, K. S. Lyngsø, K. Przyborowski, Pernille B.L. Hansen, G. Wilczyński, S. Chłopicki,
    Academic press:
    Journal of the American Heart Association (rok: 2018, tom: 7, strony: nd.), Wydawca: American Heart Association
    Status:
    Published
    DOI:
    10.1161/JAHA.117.007694 - link to the publication
  4. AFM image analysis of porous structures by means of neural networks
    Authors:
    Giergiel M., Zapotoczny B., Czyzynska-Cichon I., Konior J., Szymonski M.
    Academic press:
    Biomedical Signal Processing and Control (rok: 2021, tom: 71, strony: nd.), Wydawca: Elsevier Ltd.
    Status:
    Published
    DOI:
    10.1016/j.bspc.2021.103097 - link to the publication
  5. Quantification of fenestrations in liver sinusoidal endothelial cells by atomic force microscopy
    Authors:
    Zapotoczny B, Szafranska K, Kus E, Chlopicki S, Szymonski M.
    Academic press:
    Micron (rok: 2017, tom: 101, strony: 48–53), Wydawca: Elsevier Ltd.
    Status:
    Published
    DOI:
    10.1016/j.micron.2017.06.005 - link to the publication
  6. Retrospectively gated MRI for in vivo assessment of endothelium-dependent vasodilatation and endothelial permeability in murine models of endothelial dysfunction
    Authors:
    Bar A, Skórka T, Jasiński K, Sternak M, Bartel Z, Tyrankiewicz U, Chlopicki S
    Academic press:
    NMR Biomed (rok: 2016, tom: 29, strony: 1088-1097), Wydawca: John Wiley & Sons, Ltd.
    Status:
    Published
    DOI:
    10.1002/nbm.3567 - link to the publication
  7. Differential response of liver sinusoidal endothelial cells and hepatocytes to oleic and palmitic acid revealed by Raman and CARS imaging
    Authors:
    Matuszyk E., Sierka E., Bae H., Meyer T., Kus E., Chlopicki S., Schmitt M., Popp J., Baranska M.
    Academic press:
    Biochimica et Biophysica Acta (BBA)- Molecular Basis of Disease (rok: 2020, tom: 1866, strony: -), Wydawca: Elsevier B.V.
    Status:
    Published
    DOI:
    10.1016/j.bbadis.2020.165763 - link to the publication
  8. Raman imaging-based phenotyping of murine primary endothelial cells to identify disease-associated biochemical alterations
    Authors:
    Tott S., Grosicki M., Glowacz J., Mohaissen T., Wojnar-Lason K., Chlopicki S., Baranska M.
    Academic press:
    Biochimica et Biophysica Acta (BBA)- Molecular Basis of Disease (rok: 2021, tom: 1867, strony: nd.), Wydawca: Elsevier B.V.
    Status:
    Published
    DOI:
    10.1016/j.bbadis.2021.166180 - link to the publication
  9. Vascular Cognitive Impairment Linked to Brain Endothelium Inflammation in Early Stages of Heart Failure in Mice
    Authors:
    M.G. Adamski, M. Sternak, T. Mohaissen, D. Kaczor, J.M. Wierońska, M. Malinowska, I. Czaban, K. Byk, K. S. Lyngsø, K. Przyborowski, Pernille B.L. Hansen, G. Wilczyński, S. Chłopicki,
    Academic press:
    Journal of the American Heart Association (rok: 2018, tom: 7, strony: nd.), Wydawca: American Heart Association
    Status:
    Published
    DOI:
    10.1161/JAHA.117.007694 - link to the publication
  10. Raman spectroscopic features of primary cardiac microvascular endothelial cells(CMECs) isolated from the murine heart
    Authors:
    S. Tott, M. Grosicki, B. Klimas, D. Augustynska, S. Chlopicki, M. Baranska
    Academic press:
    Analyst (rok: 2018, tom: 143, strony: 6079–6086), Wydawca: ROYAL SOCIETY OF CHEMISTRY
    Status:
    Published
    DOI:
    10.1039/c8an01308j - link to the publication
  11. MRI-based assessment of liver perfusion and hepatocyte injury in the murine model of acute hepatitis
    Authors:
    Byk K, Jasinski K, Bartel Z, Jasztal A, Sitek B, Tomanek B, Chlopicki S, Skorka T
    Academic press:
    Magn Reson Mater Phys Biol Med (rok: 2016, tom: 29, strony: 789-798), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s10334-016-0563-2 - link to the publication
  12. Biophysical nanocharacterization of liver sinusoidal endothelial cells through atomic force microscopy
    Authors:
    Zapotoczny B., Braet F., Wisse E., Lekka M., Szymonski M.
    Academic press:
    Biophysical Reviews (rok: 2020, tom: 12, strony: 625–636), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s12551-020-00699-0 - link to the publication
  13. Raman imaging-based phenotyping of murine primary endothelial cells to identify disease-associated biochemical alterations
    Authors:
    Tott S., Grosicki M., Glowacz J., Mohaissen T., Wojnar-Lason K., Chlopicki S., Baranska M.
    Academic press:
    Biochimica et Biophysica Acta (BBA)- Molecular Basis of Disease (rok: 2021, tom: 1867, strony: nd.), Wydawca: Elsevier B.V.
    Status:
    Published
    DOI:
    10.1016/j.bbadis.2021.166180 - link to the publication
  14. AFM image analysis of porous structures by means of neural networks
    Authors:
    Giergiel M., Zapotoczny B., Czyzynska-Cichon I., Konior J., Szymonski M.
    Academic press:
    Biomedical Signal Processing and Control (rok: 2021, tom: 71, strony: nd.), Wydawca: Elsevier Ltd.
    Status:
    Published
    DOI:
    10.1016/j.bspc.2021.103097 - link to the publication
  15. Immuno-Spin Trapping-Based Detection of Oxidative Modifications in Cardiomyocytes and Coronary Endothelium in the Progression of Heart Failure in Tgαq*44 Mice
    Authors:
    Proniewski B., Czarny J., Khomich T., Kus K., Zakrzewska A., Chlopicki S.
    Academic press:
    Frontiers in Immunology (rok: 2018, tom: 9, strony: nd.), Wydawca: Frontiers
    Status:
    Published
    DOI:
    10.3389/fimmu.2018.00938 - link to the publication
  16. Short-term treatment with hepatoselective NO donor V-PYRRO/NO improves blood flow in hepatic microcirculation in liver steatosis in mice
    Authors:
    Kuś E, Jasiński K, Skórka T, Czyżyńska-Cichoń I, Chłopicki S.
    Academic press:
    Pharmacological Reports (rok: 2018, tom: 70, strony: 463 - 469), Wydawca: Elsevier Sp. z o.o.
    Status:
    Published
    DOI:
    10.1016/j.pharep.2017.11.019 - link to the publication
  17. Combined Raman- and AFM-based detection of biochemical and nanomechanical features of endothelial dysfunction in aorta isolated from ApoE/LDLR-/- mice.
    Authors:
    Maase M, Rygula A, Pacia MZ, Proniewski B, Mateuszuk L, Sternak M, Kaczor A, Chlopicki S, Kusche-Vihrog K.
    Academic press:
    Nanomedicine: Nanotechnology, Biology, and Medicine (rok: 2019, tom: 16, strony: 97-105), Wydawca: Elsevier Inc.
    Status:
    Published
    DOI:
    10.1016/j.nano.2018.11.014 - link to the publication
  18. LSEC Fenestrae Are Preserved Despite Pro-inflammatory Phenotype of Liver Sinusoidal Endothelial Cells in Mice on High Fat Diet
    Authors:
    Kus E, Kaczara P, Czyzynska-Cichon I, Szafranska K, Zapotoczny B, Kij A, Sowinska A, Kotlinowski J, Mateuszuk L, Czarnowska E, Szymonski M, Chlopicki S
    Academic press:
    Frontiers in Physiology (rok: 2019, tom: 10, strony: nd.), Wydawca: Frontiers
    Status:
    Published
    DOI:
    10.3389/fphys.2019.00006 - link to the publication
  19. Atomic Force Microscopy Reveals the Dynamic Morphology of Fenestrations in Live Liver Sinusoidal Endothelial Cells
    Authors:
    Zapotoczny B, Szafranska K, Owczarczyk K, Kus E, Chlopicki S, Szymonski M.
    Academic press:
    Scientific Reports (rok: 2017, tom: 7, strony: nd), Wydawca: Nature Publishing Group
    Status:
    Published
    DOI:
    10.1038/s41598-017-08555-0 - link to the publication
  20. Tracking Fenestrae Dynamics in Live Murine Liver Sinusoidal Endothelial Cells
    Authors:
    B. Zapotoczny, K. Szafranska, E. Kus, F. Braet, E. Wisse, S. Chlopicki, M. Szymonski
    Academic press:
    Hepatology (rok: 2018, tom: 69, strony: 876 - 888), Wydawca: American Association for the Study of Liver Diseases
    Status:
    Published
    DOI:
    10.1002/hep.30232 - link to the publication
  21. Degradation of Glycocalyx and Multiple Manifestations of Endothelial Dysfunction Coincide in the Early Phase of Endothelial Dysfunction Before Atherosclerotic Plaque Development in Apolipoprotein E/Low-Density Lipoprotein Receptor-Deficient Mice
    Authors:
    Bar A, Targosz- Korecka M, Suraj J, Proniewski B, Jasztal A, Marczyk B, Sternak M, Przybyło M, Kurpinska A, Walczak M, Kostogrys R.B, Szymonski M, Chlopicki S.
    Academic press:
    Journal of the American Heart Association (rok: 2019, tom: 8, strony: nd.), Wydawca: American Heart Association
    Status:
    Published
    DOI:
    10. - link to the publication
  22. Vibrational spectroscopy-based quantification of liver steatosis
    Authors:
    Szafraniec E, Tott S, Kus E, Augustynska D, Jasztal A, Filipek A, Chlopicki S, Baranska M
    Academic press:
    Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease (rok: 2019, tom: 1865, strony: nd.), Wydawca: Elsevier B.V.
    Status:
    Published
    DOI:
    10.1016/j.bbadis.2019.08.002 - link to the publication
  23. Morphology and force probing of primary murine liver sinusoidal endothelial cells
    Authors:
    Zapotoczny B, Owczarczyk K, Szafranska K, Kus E, Chlopicki S, Szymonski M
    Academic press:
    Journal of Molecular Recognition (rok: 2017, tom: 30, strony: 45665), Wydawca: John Wiley & Sons Ltd.
    Status:
    Published
    DOI:
    10.1002/jmr.2610 - link to the publication
  24. Label-free spectroscopic characterization of live liver sinusoidal endothelial cells (LSECs) isolated from the murine liver
    Authors:
    Kochan K, Kus E, Filipek A, Szafranka K, Chlopicki S, Baranska M
    Academic press:
    Analyst (rok: 2017, tom: 142, strony: 1308-1319), Wydawca: ROYAL SOCIETY OF CHEMISTRY
    Status:
    Published
    DOI:
    10.1039/c6an02063a - link to the publication
  25. Changes induced by non-alcoholic fatty liver disease in liver sinusoidal endothelial cells and hepatocytes: spectroscopic imaging of single live cells at the subcellular level
    Authors:
    K. Kochan, E. Kus, E. Szafraniec, A. Wislocka, S. Chlopicki, M. Baranska
    Academic press:
    Analyst (rok: 2017, tom: 142, strony: 3948-3958), Wydawca: ROYAL SOCIETY OF CHEMISTRY
    Status:
    Published
    DOI:
    10.1039/c7an00865a - link to the publication
  26. Activation pattern of ACE2/Ang-(1–7) and ACE/Ang II pathway in course of heart failure assessed by multiparametric MRI in vivo in Tgq*44 mice
    Authors:
    U. Tyrankiewicz, M. Olkowicz, T. Skórka, M. Jabłońska, A. Orzyłowska, A. Bar, M. Gonet, P. Berkowicz, K. Jasiński, J. A. Żołądź, R. T. Smoleński, S. Chłopicki
    Academic press:
    JJournal of Applied Physiology (rok: 2018, tom: 124, strony: 52 - 65), Wydawca: American Physiological Society
    Status:
    Published
    DOI:
    10.1152/japplphysiol.00571.2017 - link to the publication
  27. Physical Activity and Inhibition of ACE Additively Modulate ACE/ACE-2 Balance in Heart Failure in Mice
    Authors:
    Tyrankiewicz U., Olkowicz M., Berkowicz P., Jablonska M., Smolenski R.T., Zoladz J.A. and Chlopicki S.
    Academic press:
    Frontiers in Pharmacology (rok: 2021, tom: 12, strony: nd.), Wydawca: Frontiers Media SA
    Status:
    Published
    DOI:
    10.3389/fphar.2021.682432 - link to the publication
  28. Eicosanoid profiling in effluent of isolated perfused heart of Tgαq*44 mice with advanced heart failure.
    Authors:
    Berkowicz P, Kij A, Walczak M, Chlopicki S
    Academic press:
    Journal of Physiology and Pharmacology (rok: 2019, tom: 70, strony: 135-142), Wydawca: Polskie Towarzystwo Fizjologiczne
    Status:
    Published
    DOI:
    10.26402/jpp.2019.1.13 - link to the publication
  29. Raman spectroscopy–based insight into lipid droplets presence and contents in liver sinusoidal endothelial cells and hepatocytes
    Authors:
    E. Szafraniec, E. Kus, A. Wislocka, B. Kukla, E. Sierka, V. Untereiner, G.D. Sockalingum, S. Chlopicki, M. Baranska
    Academic press:
    Journal of Biophotonics (rok: 2018, tom: e201800290, strony: 45668), Wydawca: WILEY-VCH Verlag GmbH & Co.
    Status:
    Published
    DOI:
    10.1002/jbio.201800290 - link to the publication
  30. Multiorgan Development of Oxidative and Nitrosative Stress in LPS-Induced Endotoxemia in C57Bl/6 Mice: DHE-Based In Vivo Approach
    Authors:
    Proniewski B, Kij A, Sitek B, Kelley EE, Chlopicki S
    Academic press:
    Oxidative Medicine and Cellular Longevity (rok: 2019, tom: 2019, strony: nd.), Wydawca: Hindawi
    Status:
    Published
    DOI:
    10.1155/2019/7838406 - link to the publication
  31. Quantitative analysis methods for studying fenestrations in liver sinusoidal endothelial cells
    Authors:
    Szafranska K., Holte CF., Kruse LD., Mao H., Øie CI., Szymonski M., Zapotoczny B., McCourt AG.
    Academic press:
    Micron (rok: 2021, tom: 150, strony: nd.), Wydawca: Elsevier Ltd.
    Status:
    Published
    DOI:
    10.1016/j.micron.2021.103121 - link to the publication
  32. Enhanced cardiac hypoxic injury in atherogenic dyslipidaemia results from alterations in the energy metabolism pattern
    Authors:
    Olkowicz M, Tomczyk M, Debski J, Tyrankiewicz U, Przyborowski K, Borkowski T, Zabielska-Kaczorowska M, Szupryczynska N, Kochan Z, Smeda M, Dadlez M, Chlopicki S, Smolenski RT
    Academic press:
    Metabolism Clinical and Experimental (rok: 2021, tom: 114, strony: nd.), Wydawca: Elsevier Inc.
    Status:
    Published
    DOI:
    10.1016/j.metabol.2020.154400 - link to the publication
  33. Application of a layered model for determination of the elasticity of biological systems
    Authors:
    Rusaczonek M, Zapotoczny B, Szymonski M, Konior J
    Academic press:
    Micron (rok: 2019, tom: 124, strony: nd.), Wydawca: Elsevier Ltd.
    Status:
    Published
    DOI:
    10.1016/j.micron.2019.102705 - link to the publication
  34. Functional and Biochemical Endothelial Profiling In Vivo in a Murine Model of Endothelial Dysfunction; Comparison of Effects of 1-Methylnicotinamide and Angiotensin-converting Enzyme Inhibitor
    Authors:
    Bar A, Olkowicz M, Tyrankiewicz U, Kus E, Jasinski,K, Smolenski RT, Skorka T, Chlopicki S
    Academic press:
    Frontiers in Pharmacology (rok: 2017, tom: 8, strony: nd), Wydawca: Frontiers
    Status:
    Published
    DOI:
    10.3389/fphar.2017.00183 - link to the publication
  35. Retrospectively gated MRI for in vivo assessment of endothelium-dependent vasodilatation and endothelial permeability in murine models of endothelial dysfunction
    Authors:
    Bar A, Skórka T, Jasiński K, Sternak M, Bartel Z, Tyrankiewicz U, Chlopicki S
    Academic press:
    NMR Biomed (rok: 2016, tom: 29, strony: 1088-1097), Wydawca: John Wiley & Sons, Ltd.
    Status:
    Published
    DOI:
    10.1002/nbm.3567 - link to the publication
  36. Quantification of fenestrations in liver sinusoidal endothelial cells by atomic force microscopy
    Authors:
    Zapotoczny B, Szafranska K, Kus E, Chlopicki S, Szymonski M.
    Academic press:
    Micron (rok: 2017, tom: 101, strony: 48–53), Wydawca: Elsevier Ltd.
    Status:
    Published
    DOI:
    10.1016/j.micron.2017.06.005 - link to the publication

O Narodowym Centrum Nauki

Finansowanie nauki

Współpraca zagraniczna

Centrum prasowe

Deklaracja dostępności
Przetwarzanie danych osobowych
Polityka cookies

Partnerzy