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New regioselective chemical and biochemical strategies for proteins labeling

2019/33/B/ST4/02428

Keywords

Descriptors

  • NZ1_002: Biochemia
  • NZ1_003: Biofizyka
  • ST4_009: Chemia biologiczna

Panel

ST4 – Chemistry: physical chemistry/chemical physics, theoretical chemistry, analytical chemistry, inorganic chemistry, organic chemistry, method development

Host institution

Uniwersytet Wrocławski, Wydział Biotechnologii

Voivodeship: dolnośląskie

Other projects carried out by the institution »

Principal investigator (from the host institution)

prof. Artur Krężel

Number of co-investigators in the project: 8

Call

OPUS 17 – announced on 2019-03-15

Amount awarded
2 237 000 PLN
Project start date
2020-01-30
Project end date
2024-04-29
Project duration
51 months (the same as in the proposal)
Project status
Project settled

Project description

Download the project description in a pdf file

Note — project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Information in the final report

Publication in academic press/journals (7)

  1. One-Step Sortase-Mediated Chemoenzymatic Semisynthesis of Deubiquitinase-Resistant Ub-Peptide Conjugates

    Authors:
    Avinash Kumar Singh, Sumit Murmu, Artur Krężel
    Academic press:
    ACS Omega (year: 2022, volume: 7, pages: 46693–46701), Publisher: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/acsomega.2c05652 – link to the publication

  2. Structures of Silver Fingers and a Pathway to Their Genotoxicity

    Authors:
    Katarzyna Kluska, Giulia Veronesi, Aurélien Deniaud, Bálint Hajdu, Béla Gyurcsik, Wojciech Bal Artur Krężel
    Academic press:
    Angewandte Chemie (year: 2022, volume: 61, pages: e202116621), Publisher: Wiley
    Status:
    Published
    DOI:
    10.1002/ange.202116621 – link to the publication

  3. OaAEP1 ligase-assisted chemoenzymatic synthesis of full cysteine-rich metal-binding cyanobacterial metallothionein SmtA

    Authors:
    Anastasiia Antonenko, Avinash Kumar Singh, Karolina Mosna, Artur Krężel
    Academic press:
    Bioconjugate Chemistry (year: 2023, volume: nd, pages: nd), Publisher: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/acs.bioconjchem.3c00037 – link to the publication

  4. Calcium-assisted sortase A cleavage of SUMOylated metallothionein constructs leads to high-yield production of human MT3

    Authors:
    Avinash Kumar Singh, Artur Krężel
    Academic press:
    Microbial Cell Factories (year: 2023, volume: 22, pages: 125), Publisher: Springer Nature
    Status:
    Published
    DOI:
    10.1186/s12934-023-02134-x – link to the publication

  5. Biarsenical fluorescent probes for multifunctional site-specific modification of proteins applicable in life sciences: An overview and future outlook

    Authors:
    Adam Pomorski, Artur Krężel
    Academic press:
    Metallomics (year: 2020, volume: 12, pages: 1179-1207), Publisher: Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/D0MT00093K – link to the publication

  6. A combined biochemical and cellular approach reveals Zn2+-dependent hetero- and homodimeric CD4 and Lck assemblies in T cells

    Authors:
    Anna M. Kocyła, Aleksander Czogalla, Inga Wessels, Lothar Rink, Artur Krężel
    Academic press:
    Structure (year: 2024, volume: 32, pages: 292-303), Publisher: Cell Press
    Status:
    Published
    DOI:
    10.1016/j.str.2023.11.013 – link to the publication

  7. A guide to good practice in chemometric methods for vibrational spectroscopy, electrochemistry, and hyphenated mass spectrometry

    Authors:
    Manuel David Peris-Díaz, Artur Krężel
    Academic press:
    Trends in Analytical Chemistry (year: 2021, volume: 135, pages: 116157), Publisher: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.trac.2020.116157 – link to the publication

Articles in post-conference publications (13)

  1. Ligaza OaAEP1 jako narzędzie molekularne do tworzenia wiązania peptydowego

    Authors:
    Anastasiia Antonenko, Avinash Kumar Singh, Artur Krężel
    Conference:
    Książka Abstraktów 2020/2021 63. Zjazd naukowy Polskiego Toważystwa Chemicznego (year: 2021, volume: 63. Zjazd naukowy PTCHEM, pages: 438), Publisher: PTCHEM
    Date:
    conference 13-17 Września 2021
    Status:
    Published

  2. Oddziaływania izoform ludzkich metalotionein z jednowartościowymi jonami miedzi

    Authors:
    Alicja Misiaszek, Artur Krężel
    Conference:
    Książka Abstraktów 2022. 64. Zjazd Naukowy Polskiego Towarzystwa Chemicznego (year: 2022, volume: 64. Zjazd Naukowy PTChem, pages: 156), Publisher: PTChem
    Date:
    conference 11-16 września 2022
    Status:
    Published

  3. REGULACJA I DEREGULACJA FUNKCJI BIAŁEK CYNKOWYCH – WALKA DOBREGO Z DOBRYM I ZŁYM

    Authors:
    Artur Krężel
    Conference:
    Książka Abstraktów 2022. 64. Zjazd Naukowy Polskiego Towarzystwa Chemicznego (year: 2022, volume: 64. Zjazd Naukowy PTChem, pages: 113), Publisher: PTChem
    Date:
    conference 11-16 września 2022
    Status:
    Published

  4. New chemoenzymatic approach for the semisynthesis and site-specifically ubiquitination of alpha-synuclein and its influence on its aggregation

    Authors:
    Anastasiia Antonenko, Avinash Kumar Singh, Artur Krężel
    Conference:
    EFMC-ISCB Book of Abstracts (year: 2023, volume: International Symposium on Chemical Biology, pages: 128), Publisher: EFMC
    Date:
    conference 16-18.11.2023
    Status:
    Published

  5. CHEMICAL OXIDATION OF P53 MONITORED BY MASS SPECTROMETRY

    Authors:
    Manuel Peris Diaz, Perdita Barran, Artur Krężel
    Conference:
    IMSC 2022 Abstract Book (year: 2022, volume: 24th IMSC 2022: International Mass Spectrometry Conference, pages: 1028-1029), Publisher: International Mass Spectrometry Foundation (IMSF)
    Date:
    conference 27 sierpnia - 2 września 2022
    Status:
    Published

  6. Specificity factors that govern zinc-dependent assembly of CD4 coreceptor and Lck tyrosine kinase

    Authors:
    Anna Kocyla, Aleksander Czogalla, and Artur Krężel
    Conference:
    BioMetals 2020 (year: 2020, volume: 12th International BioMetals), Publisher: Université Grenoble Alpes
    Date:
    conference 6-10 lipca 2020
    Status:
    Published

  7. Highly efficient sortase-A and Ulp1-mediated purification method for the expression and purification of recombinant human MT3 in bacterial system

    Authors:
    Avinash Kumar Singh, Artur Krezel
    Conference:
    Abstracts of papers presented at the EMBO Workshop: Chemical biology 2022 (year: 2022, volume: EMBO Workshop: Chemical biology 2022 EMBL Advanced Training Centre and virtual, pages: 173), Publisher: EMBL Advanced Training Centre, Heidelberg
    Date:
    conference 5-8 września 2022
    Status:
    Published

  8. New efficient enzymatic strategy for the semisynthesis of metallothionein

    Authors:
    Anastasiia Antonenko, Avinash Kumar Singh, Artur Krezel
    Conference:
    Abstracts of papers presented at the EMBO Workshop: Chemical biology 2022 (year: 2022, volume: EMBO Workshop: Chemical biology 2022, EMBL Advanced Training Centre and virtual, pages: 66), Publisher: EMBL Advanced Training Centre, Heidelberg
    Date:
    conference 5-8 września 2022
    Status:
    Published

  9. Site-Specific sortase-mediated ubiquitination and sumoylation of proteins using fluorescent biarsenical probes

    Authors:
    Anastasiia Antonenko, Adam Pomorski, Avinash Kumar Singh, Artur Krężel
    Conference:
    EFMC-ISCB Book of Abstracts (year: 2023, volume: EFMC International Symposium on Chemical Biology, pages: 129), Publisher: EFMC
    Date:
    conference 16-18.11.2023
    Status:
    Published

  10. Zinc to silver swap in zinc finger domains disrupts their native structures and DNA recognition

    Authors:
    Artur Krężel
    Conference:
    ICTEM 2022 Program and Abstract Book (year: 2022, volume: ICTEM 2022: International Conference of Trace Elements and Minerals, pages: 67), Publisher: Uniklinik RWTH Aachen
    Date:
    conference 5 czerwca - 10 czerwca 2022
    Status:
    Published

  11. Semisynthesis of SmtA metallothionein using a highly efficient OaAEP1 ligase – comparison of metal binding properties of synthetic and E. coli-produced protein

    Authors:
    Anastasiia Antonenko, Avinash Kumar Singh, Artur Krężel
    Conference:
    EuroBIC16 Abstract Book (year: 2022, volume: EuroBIC16: 16th European Biological Inorganic Chemistry Conference, pages: brak danych), Publisher: University Grenoble Alpes
    Date:
    conference 17-21 lipca 2022
    Status:
    Published

  12. WYKORZYSTANIE WYSOKOEFEKTYWNEJ LIGAZY ASPARAGINOWEJ DO SEMISYNTEZY BIAŁEK

    Authors:
    Anastasiia Antonenko, Avinash Kumar Singh, Artur Krężel
    Conference:
    Książka Abstraktów. 64. Zjazd Naukowy Polskiego Towarzystwa Chemicznego (year: 2022, volume: 64. Zjazd Naukowy PTChem, pages: 155), Publisher: PTChem
    Date:
    conference 11-16 września
    Status:
    Published

  13. Wiązanie jonów Cu(I) i Zn(II) przez ludzką metalotioneinę-3 (MT3) i jej ubikwitynylowaną formę

    Authors:
    Alicja Orzeł, Manuel Peris Diaz, Avinash Kumar Singh, Artur Krężel
    Conference:
    Książka Abstraktów (year: 2023, volume: 65. Zjazd Naukowy PTChem, pages: 160), Publisher: Polskie Towarzystwo Chemiczne
    Date:
    conference 19-22.09.2023
    Status:
    Published

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Graphic design and implementation: Jakub Piotrowski