Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

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Towards understanding the cellular fate of exogenously delivered mRNA

2018/31/D/NZ1/03526

Keywords:

cap structure RNA sensing immunogenicity in vitro transcription RNA-seq mRNP complexes

Descriptors:

  • NZ1_1: Molecular biology
  • NZ1_2: Biochemistry
  • ST5_16:

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

Uniwersytet Warszawski, Centrum Nowych Technologii Uniwersytetu Warszawskiego

woj. mazowieckie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr hab. Paweł Sikorski 

Number of co-investigators in the project: 4

Call: SONATA 14 - announced on 2018-09-14

Amount awarded: 1 800 866 PLN

Project start date (Y-m-d): 2019-07-11

Project end date (Y-m-d): 2023-05-10

Project duration:: 46 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. Aparat do elektrotransferu.
  2. komputer + oprogramowanie (podstawowy pakiet biurowy oraz pakiet oprogramowania naukowego) (2 szt.) (20 000 PLN)
  3. wysoko rozdzielcza elektroforeza kwasów nukleinowych typu lab-on-chip (190 000 PLN)

Information in the final report

  • Publication in academic press/journals (4)
  1. Ethylenediamine derivatives efficiently react with oxidized RNA 3′ ends providing access to mono and dually labelled RNA probes for enzymatic assays and in vivo translation
    Authors:
    Adam Mamot, Pawel J. Sikorski, Aleksandra Siekierska, Peter de Witte, Joanna Kowalska, Jacek Jemielity
    Academic press:
    Nucleic Acids Research (rok: 2022, tom: 50(1), strony: e3), Wydawca: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/nar/gkab867 - link to the publication
  2. The identity and methylation status of the first transcribed nucleotide in eukaryotic mRNA 5′ cap modulates protein expression in living cells
    Authors:
    Pawel J Sikorski*, Marcin Warminski, Dorota Kubacka, Tomasz Ratajczak, Dominika Nowis, Joanna Kowalska*, Jacek Jemielity*; * - corresponding author
    Academic press:
    Nucleic Acids Research (rok: 2020, tom: 48(4), strony: 1607-1626), Wydawca: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/nar/gkaa032 - link to the publication
  3. 2′-O-Methylation of the second transcribed nucleotide within the mRNA 5′ cap impacts the protein production level in a cell-specific manner and contributes to RNA immune evasion
    Authors:
    Karolina Drazkowska, Rafal Tomecki, Marcin Warminski, Natalia Baran, Dominik Cysewski, Anaïs Depaix, Renata Kasprzyk, Joanna Kowalska, Jacek Jemielity, Pawel J. Sikorski*; * - corresponding author
    Academic press:
    Nucleic Acids Research (rok: 2022, tom: 50(16), strony: 9051–9071), Wydawca: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/nar/gkac722 - link to the publication
  4. Tricks and threats of RNA viruses - towards understanding the fate of viral RNA
    Authors:
    Lukasz Markiewicz, Karolina Drazkowska, Pawel J. Sikorski*; * - corresponding author
    Academic press:
    RNA Biology (rok: 2021, tom: 18(5), strony: nie dotyczy), Wydawca: Taylor & Francis Group
    Status:
    Published
    DOI:
    10.1080/15476286.2021.1875680 - link to the publication

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