Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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The contribution of ultrashort electric pulses in the induction of oxidative stress and to assessment of the anticancer potential of nanosecond electroporation (nsPEF) in colon adenocarcinoma model in vitro and in vivo

2016/22/E/NZ5/00671

Keywords:

nanosecond electroporation oxidative stress free radicals lipid peroxidation protein damage colon adenocarcinoma

Descriptors:

  • NZ3_3: Apoptosis
  • NZ5_7: Human disease treatment
  • NZ7_12: Prevention of human diseases

Panel:

NZ5 - Human and animal noninfectious diseases: etiology, mechanisms, diagnosis and treatment of diseases, poisonings and injuries (without neurological diseases)

Host institution :

Uniwersytet Medyczny im. Piastów Śląskich we Wrocławiu, Wydział Farmaceutyczny

woj. dolnośląskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr hab. Julita Kulbacka 

Number of co-investigators in the project: 7

Call: SONATA BIS 6 - announced on 2016-06-15

Amount awarded: 1 838 800 PLN

Project start date (Y-m-d): 2017-04-20

Project end date (Y-m-d): 2022-04-19

Project duration:: 60 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. Oscyloskop cyfrowy z sondą wysokonapięciową do pomiaru sygnału generowanego przez nanopulsator (50 000 PLN)
  2. Mały cytometr przepływowy (200 000 PLN)
  3. oprogramowanie GraphPad Prism (3 000 PLN)
  4. Wielofunkcyjny czytnik mikropłytek.
  5. Elektroda do generatora nanosekundowych pulsów (5 000 PLN)

Information in the final report

  • Publication in academic press/journals (14)
  • Articles in post-conference publications (8)
  • Book publications / chapters in book publications (1)
  1. An overview of paraneoplastic neurological syndromes – pathophysiology and clinical insight
    Authors:
    Stanisław Kwiatkowski, Karolina Kolasińska , Bartosz Knap , Dawid Przystupski , Krzysztof Kotowski , Weronika Bartosik , Julita Kulbacka
    Academic press:
    World Scientific News (rok: 2018, tom: 108, strony: 87-98), Wydawca: Scientific Publishing House
    Status:
    Published
    DOI:
    brak - link to the publication
  2. Curcumin Loaded Nanocarriers with Varying Charges Augmented with Electroporation Designed for Colon Cancer Therapy
    Authors:
    Julita Kulbacka, Kazimiera A. Wilk, Urszula Bazylińska, Magda Dubińska Magiera, Stanisław Poteczek, Jolanta Saczko
    Academic press:
    International Journal of Molecular Sciences (rok: 2021, tom: 23(3), strony: 1377), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms23031377 - link to the publication
  3. Proapoptotic activity induced by photodynamic reaction with novel cyanine dyes in caspase-3-deficient human breast adenocarcinoma cell lines (MCF/ WT and MCF/DX)
    Authors:
    Julita Kulbacka, Anna Choromańska, Małgorzata Drąg-Zalesińska, Piotr Nowak, Dagmara Baczyńska, Małgorzata Kotulska, Iwona Bednarz-Misa, Jolanta Saczko, Agnieszka Chwiłkowska
    Academic press:
    Photodiagnosis and Photodynamic Therapy (rok: 2020, tom: 30, strony: 101775), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.pdpdt.2020.101775 - link to the publication
  4. In search of panacea - review of recent studies concerning nature-derived anticancer agents
    Authors:
    Dawid Przystupski, Magdalena J. Niemczura, Agata Górska, Stanisław Supplitt, Krzysztof Kotowski, Piotr Wawryka, Paulina Rozborska, Kinga Woźniak, Olga Michel, Aleksander Kiełbik, Weronika Bartosik, Jolanta Saczko, Julita Kulbacka
    Academic press:
    Nutrients (rok: 2019, tom: 11(6), strony: 38), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/nu11061426 - link to the publication
  5. Connection between Warburg Effect and Oncometabolites Biosynthesis with its Clinical Implications
    Authors:
    Wojciech Szlasa, Kamila Wala, Aleksander Kiełbik, Aleksandra Zalesińska, Jolanta Saczko, Julita Kulbacka
    Academic press:
    Organic and Medicinal Chemistry International Journal (rok: 2020, tom: 9(5), strony: 555771), Wydawca: JP Juniper Publishers
    Status:
    Published
    DOI:
    10.19080/OMCIJ.2020.09.555771 - link to the publication
  6. Ultrathin glass fiber microprobe for electroporation of arbitrary selected cell groups
    Authors:
    Julita Kulbacka, Rafał Kasztelanic, Małgorzata Kotulska, Dariusz Pysz, Grzegorz Stępniewski, Ryszard Stępień, Jolanta Saczko, Damijan Miklavcic, Ryszard Buczyński
    Academic press:
    Bioelectrochemistry (rok: 2020, tom: 135, strony: 107545), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bioelechem.2020.107545 - link to the publication
  7. Nanoelectropulse delivery for cell membrane perturbation and oxidation in human colon adenocarcinoma cells with drug resistance
    Authors:
    Julita Kulbacka, Anna Choromańska, Anna Szewczyk, Olga Michel, Dagmara Baczyńska, Andrzej Sikora, Joanna Rossowska, Marek Kulbacki, Nina Rembiałkowska
    Academic press:
    Bioelectrochemistry (rok: 2022, ), Wydawca: Elsevier
    Status:
    Submitted
    DOI:
    brak - link to the publication
  8. The First Application of Nanoelectrochemotherapy in Feline Oral Malignant Melanoma Treatment—Case Study
    Authors:
    Joanna Tunikowska, Agnieszka Antończyk, Nina Rembiałkowska, Łukasz Jóźwiak, Vitalij Novickij, Julita Kulbacka
    Academic press:
    Animals (rok: 2020, tom: 10, strony: 556), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ani10040556 - link to the publication
  9. 3PO as a Selective Inhibitor of 6-Phosphofructo- 2-Kinase/Fructose-2,6-Biphosphatase 3 in A375 Human Melanoma Cells
    Authors:
    Krzysztof Kotowski, Stanisław Supplitt, Daniel Wiczew, Dawid Przystupski, Weronika Bartosik, Jolanta Saczko, Joanna Rossowska, Małgorzata Drąg-Zalesińska, Olga Michel, Julita Kulbacka
    Academic press:
    Anticancer Research (rok: 2020, tom: 40, strony: 2613-2625), Wydawca: International Institute of Anticancer Research
    Status:
    Published
    DOI:
    10.21873/anticanres.14232 - link to the publication
  10. The First Study of Irreversible Electroporation with Calcium Ions and Chemotherapy in Patients with Locally Advanced Pancreatic Adenocarcinoma
    Authors:
    Julia Rudno-Rudzińska, Wojciech Kielan, Maciej Guziński, Maciej Płochocki and Julita Kulbacka
    Academic press:
    Applied Sciences (rok: 2020, tom: 10, strony: 5163), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/app10155163 - link to the publication
  11. The Impact of Extracellular Ca2+ and Nanosecond Electric Pulses on Sensitive and Drug-Resistant Human Breast and Colon Cancer Cells
    Authors:
    Julita Kulbacka, Nina Rembiałkowska, Anna Szewczyk, Helena Moreira, Anna Szyjka,Irutė Girkontaitė, Kamil P. Grela, Vitalij Novickij
    Academic press:
    Cancers (rok: 2021, tom: 13, strony: 3216), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/cancers13133216 - link to the publication
  12. Bioluminescence as a sensitive electroporation indicator in submicrosecond and microsecond range of electrical pulses
    Authors:
    Vitalij Novickij, Auksė Zinkevičienė, Veronika Malyško, Jurij Novickij, Julita Kulbacka, Nina Rembialkowska, Irutė Girkontaitė
    Academic press:
    Journal of Photochemistry & Photobiology, B: Biology (rok: 2020, tom: 213, strony: 112066), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.jphotobiol.2020.112066 - link to the publication
  13. Effects of extracellular medium conductivity on cell response in the context of sub-microsecond range calcium electroporation
    Authors:
    Vitalij Novickij, Nina Rembialkowska, Gediminas Staigvila and Julita Kulbacka
    Academic press:
    Scientific Reports (rok: 2020, tom: 10, strony: 3718), Wydawca: Nature
    Status:
    Published
    DOI:
    10.1038/s41598-020-60789-7 - link to the publication
  14. Lipid composition of the cancer cell membrane
    Authors:
    Wojciech Szlasa, Iga Zenran, Aleksandra Zalesińska, Mounir Tarek, Julita Kulbacka
    Academic press:
    Journal of Bioenergetics and Biomembranes (rok: 2020, tom: 52, strony: 321-342), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s10863-020-09846-4 - link to the publication
  1. Image Analysis to Study Cytoskeleton Alterations in Cancer Cells Exposed to Nanosecond Pulsed Electric Field
    Authors:
    Julita Kulbacka, Marek Kulbacki, Jakub Segen, Anna Szewczyk, Grzegorz Chodaczek, Anna Choromańska, Nina Rembiałkowska, Olga Michel, Ryszard Klempous, Jolanta Saczko
    Conference:
    INES 2018 : IEEE 22nd International Conference on Intelligent Engineering Systems (rok: 2018, ), Wydawca: IEEE
    Data:
    konferencja 21-23.06.2018
    Status:
    Published
  2. YM-1 as a modulator of HSP70 protein in chemotherapy combined with 5-fluorouracil
    Authors:
    Kamila Majgier, Julita Kulbacka
    Conference:
    4th International Wroclaw Scientific Meetings (rok: 2020, ), Wydawca: Tygiel
    Data:
    konferencja 09-10.10.2020
    Status:
    Published
  3. Antitumor efficacy of ultra-short electrical pulses in murine colon cancer (MC38/0) in vivo
    Authors:
    Julita Kulbacka, Natalia Anger-Góra, Nina Rembiałkowska, Joanna Rossowska
    Conference:
    4th International Wroclaw Scientific Meetings (rok: 2020, ), Wydawca: Tygiel
    Data:
    konferencja 09-10.10.2020
    Status:
    Published
  4. Milli-, Micro, and Nanosecond PEF in Gastrointestinal Related Cancers - in vitro and in vivo Models
    Authors:
    Julita Kulbacka, Nina Rembiałkowska, Joanna Rossowska, Julia Rudno-Rudzińska3, Anna Szewczyk, Anna Choromańska, Olga Michel, Agnieszka Chwiłkowska, Jolanta Saczko & Vitalij Novickij
    Conference:
    XXVIth International Symposium on Bioelectrochemistry and Bioenergetics (rok: 2021, ), Wydawca: The Bioelectrochemical Society
    Data:
    konferencja 2021.05.09-13
    Status:
    Published
  5. Oxidative stress in gastric adenocarcinoma cells after nanosecond PEF exposure
    Authors:
    Julita Kulbacka, Nina Rembiałkowska, Anna Szewczyk, Joanna Rossowska, Jolanta Saczko, Małgorzata Drąg-Zalesińska, Anna Choromańska
    Conference:
    2nd World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine, and Food & Environmental Technologies inco[r]porating Bioelectrics 2017 - The 14th International Bioelectrics Symposium (rok: 2017, ), Wydawca: Virginia, Norfolk, USA
    Data:
    konferencja 24-28 września
    Status:
    Published
  6. Cell membrane permeabilization in glioma cells to support enhanced drug delivery
    Authors:
    Natalia Niedzielska, Emilia Krzywiecka, Małgorzata Kotulska, Sebastian Kraszewski, Agnieszka Chwiłkowska, Jolanta Saczko, Anna Choromańska, Olga Michel, Anna Szewczyk, Nina Rembiałkowska, Julita Kulbacka
    Conference:
    5th European Joint Theoretical/Experimental Meeting on Membranes (rok: 2017, ), Wydawca: Acta Biochimica Polonica
    Data:
    konferencja 6-8 grudnia
    Status:
    Published
  7. Modulating drug resistance prior to electrochemotherapy - in vitro study with green tea bioflavonoid on primary cells and cell lines from pancreatic cancer
    Authors:
    Olga Michel, Justyna Mączyńska, Piotr Błasiak, Adam Rzechonek, Joanna Rossowska, Jolanta Saczko, Julita Kulbacka
    Conference:
    2nd World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine, and Food & Environmental Technologies inco[r]porating Bioelectrics 2017 - The 14th International Bioelectrics Symposium. (rok: 2017, ), Wydawca: Virginia, Norfolk, USA
    Data:
    konferencja 24-28 września
    Status:
    Published
  8. Calcium electroporation stimulates ROS release and alternated ASPH expression in human colon cancer
    Authors:
    Anna Szewczyk, Nina Rembiałkowska, Anna Choromańska, Katarzyna Bieżuńska-Kusiak, Jolanta Saczko, Julita Kulbacka
    Conference:
    XXVIth International Symposium on Bioelectrochemistry and Bioenergetics (rok: 2021, ), Wydawca: The Bioelectrochemical Society
    Data:
    konferencja 2021.05.09-13
    Status:
    Published
  1. Cell membrane transport mechanisms: ion channels and electrical properties of cell membranes
    Authors:
    Julita Kulbacka, Anna Choromańska, Joanna Rossowska, Joanna Weżgowiec, Jolanta Saczko, Marie-Pierre Rols
    Book:
    Adv.Anat.Embryol.Cell Biol. (rok: 2017, tom: 227, strony: 39-58), Wydawca: Springer International Publishing AG
    Status:
    Published

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