Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Identification of CAG repeat-binding proteins and imaging RNA-protein interactions in cells

2015/19/B/NZ2/02453

Keywords:

polyglutamine diseases RNA toxicity RNA-protein interactions quantitative proteomics RNA imaging

Descriptors:

  • NZ1_1: Molecular biology
  • NZ2_3: Proteomics
  • NZ5_3: Pathogenesis of human diseases

Panel:

NZ2 - Genetics, genomics: molecular genetics, genomics, proteomics, bioinformatics, systems biology, genetic epidemiology

Host institution :

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

woj. wielkopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr Adam Ciesiołka 

Number of co-investigators in the project: 7

Call: OPUS 10 - announced on 2015-09-15

Amount awarded: 1 899 440 PLN

Project start date (Y-m-d): 2016-06-20

Project end date (Y-m-d): 2021-12-19

Project duration:: 66 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. termocykler (30 000 PLN)
  2. orbitalna wytrząsarka laboratoryjna (5 000 PLN)
  3. aparat do elektroforezy i transferu białek (11 000 PLN)
  4. dewar (10 000 PLN)
  5. mikrowirówka (5 000 PLN)
  6. serwer do przechowywania danych proteomicznych i zdjęć mikroskopowych (12 000 PLN)
  7. sprzęt komputerowy z oprogramowaniem (2 szt.) (10 000 PLN)
  8. koncentrator próżniowy (28 000 PLN)
  9. licencja Corel Draw.
  10. nukleofektor (83 000 PLN)
  11. wysokoprzepustowy sonikator (72 000 PLN)

Information in the final report

  • Publication in academic press/journals (12)
  • Articles in post-conference publications (1)
  1. Artificial miRNAs targeting CAG repeat expansion in ORFs cause rapid deadenylation and translation inhibition of mutant transcripts
    Authors:
    Ciesiolka, A., Stroynowska-Czerwinska, A., Joachimiak, P., Ciolak, A., Kozlowska, E., Michalak, M., Dabrowska, M., Olejniczak, M., Raczynska, K. D., Zielinska, D., Wozna-Wysocka, M., Krzyzosiak, W. J., Fiszer, A.
    Academic press:
    Cellular and Molecular Life Sciences (rok: 2021, tom: 78, strony: 1577-1596), Wydawca: Springer Science+Business Media
    Status:
    Published
    DOI:
    10.1007/s00018-020-03596-7 - link to the publication
  2. Generation of human iPS cell line IBCHi002-A from spinocerebellar ataxia type 3/Machado-Joseph disease patient's fibroblasts
    Authors:
    Ciolak, A., Krzyzosiak, W. J., Kozlowska, E., Fiszer, A.
    Academic press:
    Stem Cell Research (rok: 2020, tom: 45, strony: 101796), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.scr.2020.101796 - link to the publication
  3. Nuclear speckles: molecular organization, biological function and role in Disease
    Authors:
    Galganski L., Urbanek M.O., Krzyzosiak W.J.
    Academic press:
    Nucleic Acids Research (rok: 2017, tom: 45, strony: 10350-10368), Wydawca: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/nar/gkx759 - link to the publication
  4. RAN Translation of the Expanded CAG Repeats in the SCA3 Disease Context
    Authors:
    Jazurek-Ciesiolka, M., Ciesiolka, A., Komur, A. A., Urbanek-Trzeciak, M. O., Krzyzosiak, W. J., Fiszer A.
    Academic press:
    Journal of Molecular Biology (rok: 2020, tom: 432, strony: 166699), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.033 - link to the publication
  5. Reduction of Huntington's Disease RNA Foci by CAG Repeat-Targeting Reagents
    Authors:
    Urbanek M.O., Fiszer A., Krzyzosiak W.J.
    Academic press:
    Frontiers in Cellular Neuroscience (rok: 2017, tom: 11, strony: 82), Wydawca: Frontiers Media SA
    Status:
    Published
    DOI:
    10.3389/fncel.2017.00082 - link to the publication
  6. 2D and 3D FISH of expanded repeat RNAs in human lymphoblasts
    Authors:
    Urbanek M.O., Michalak M., Krzyzosiak W.J.
    Academic press:
    Methods (rok: 2017, tom: 120, strony: 49-57), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.ymeth.2017.04.002 - link to the publication
  7. Structural Characteristics of Simple RNA Repeats Associated with Disease and their Deleterious Protein Interactions
    Authors:
    Ciesiolka A., Jazurek M., Drazkowska K., Krzyzosiak W.J.
    Academic press:
    Frontiers in Cellular Neuroscience (rok: 2017, tom: 11, strony: 97), Wydawca: Frontiers Media SA
    Status:
    Published
    DOI:
    10.3389/fncel.2017.00097 - link to the publication
  8. Discriminating RNA variants with single-molecule allele-specific FISH
    Authors:
    Urbanek M.O., Krzyzosiak W.J.
    Academic press:
    Mutation Research – Reviews in Mutation Research (rok: 2017, tom: 773, strony: 230-241), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.mrrev.2016.09.003 - link to the publication
  9. Generation of New Isogenic Models of Huntington's Disease Using CRISPR-Cas9 Technology
    Authors:
    Dabrowska, M., Ciolak, A., Kozlowska, E., Fiszer, A., Olejniczak, M.
    Academic press:
    International Journal of Molecular Sciences (rok: 2020, tom: 21, strony: 1854), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms21051854 - link to the publication
  10. miRNAmotif—A Tool for the Prediction of Pre-miRNA–Protein Interactions
    Authors:
    Martyna O. Urbanek-Trzeciak, Edyta Jaworska, Wlodzimierz J. Krzyzosiak
    Academic press:
    International Journal of Molecular Sciences (rok: 2018, tom: 19, strony: 4075), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms19124075 - link to the publication
  11. Identifying proteins that bind to specific RNAs - focus on simple repeat expansion diseases
    Authors:
    Jazurek M., Ciesiolka A., Starega-Roslan J., Bilinska K., Krzyzosiak W.J.
    Academic press:
    Nucleic Acids Research (rok: 2016, tom: 44, strony: 9050 – 9070), Wydawca: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/nar/gkw803 - link to the publication
  12. Implications of Poly(A) Tail Processing in Repeat Expansion Diseases
    Authors:
    Joachimiak P., Ciesiolka A., Figura G., Fiszer A.
    Academic press:
    Cells (rok: 2022, tom: 11, strony: 677), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/cells11040677 - link to the publication
  1. Ribosome meets RISC at expanded CAG repeat tract - allele-selective RNAi approach for therapy of polyglutamine diseases
    Authors:
    P. Joachimiak, A. Ciesiolka, A. Luzna, E. Kozlowska, M. Michalak, W.J. Krzyzosiak, A. Fiszer
    Conference:
    The 44th FEBS Congress: From molecules to living systems (rok: 2019, ), Wydawca: John Wiley & Sons on behalf of the Federation of European Biochemical Societies
    Data:
    konferencja 43622
    Status:
    Published

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