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Identification of CAG repeat-binding proteins and imaging RNA-protein interactions in cells

2015/19/B/NZ2/02453

Keywords

Descriptors

  • NZ1_1: Biologia molekularna
  • NZ2_3: Proteomika
  • NZ5_3: Patogeneza chorób człowieka

Panel

NZ2 – Genetics, genomics: molecular genetics, genomics, proteomics, bioinformatics, systems biology, genetic epidemiology

Host institution

Instytut Chemii Bioorganicznej PAN

Voivodeship: wielkopolskie

Other projects carried out by the institution »

Principal investigator (from the host institution)

dr Adam Kazimierz Ciesiołka

Number of co-investigators in the project: 7

Call

OPUS 10 – announced on 2015-09-15

Amount awarded
1 899 440 PLN
Project start date
2016-06-20
Project end date
2021-12-19
Project duration
66 months (the same as in the proposal)
Project status
Project settled

Project description

Download the project description in a pdf file

Note — project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. aparat do elektroforezy i transferu białek. Cost: 11 000 PLN
  2. dewar. Cost: 10 000 PLN
  3. koncentrator próżniowy. Cost: 28 000 PLN
  4. licencja Corel Draw.
  5. mikrowirówka. Cost: 5 000 PLN
  6. nukleofektor. Cost: 83 000 PLN
  7. orbitalna wytrząsarka laboratoryjna. Cost: 5 000 PLN
  8. serwer do przechowywania danych proteomicznych i zdjęć mikroskopowych. Cost: 12 000 PLN
  9. sprzęt komputerowy z oprogramowaniem (2 pcs). Cost: 10 000 PLN
  10. termocykler. Cost: 30 000 PLN
  11. wysokoprzepustowy sonikator. Cost: 72 000 PLN

Information in the final report

Publication in academic press/journals (12)

  1. RAN Translation of the Expanded CAG Repeats in the SCA3 Disease Context

    Authors:
    Jazurek-Ciesiolka, M., Ciesiolka, A., Komur, A. A., Urbanek-Trzeciak, M. O., Krzyzosiak, W. J., Fiszer A.
    Academic press:
    Journal of Molecular Biology (year: 2020, volume: 432, pages: 166699), Publisher: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.jmb.2020.10.033 – link to the publication

  2. miRNAmotif—A Tool for the Prediction of Pre-miRNA–Protein Interactions

    Authors:
    Martyna O. Urbanek-Trzeciak, Edyta Jaworska, Wlodzimierz J. Krzyzosiak
    Academic press:
    International Journal of Molecular Sciences (year: 2018, volume: 19, pages: 4075), Publisher: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms19124075 – link to the publication

  3. Implications of Poly(A) Tail Processing in Repeat Expansion Diseases

    Authors:
    Joachimiak P., Ciesiolka A., Figura G., Fiszer A.
    Academic press:
    Cells (year: 2022, volume: 11, pages: 677), Publisher: MDPI
    Status:
    Published
    DOI:
    10.3390/cells11040677 – link to the publication

  4. Artificial miRNAs targeting CAG repeat expansion in ORFs cause rapid deadenylation and translation inhibition of mutant transcripts

    Authors:
    Ciesiolka, A., Stroynowska-Czerwinska, A., Joachimiak, P., Ciolak, A., Kozlowska, E., Michalak, M., Dabrowska, M., Olejniczak, M., Raczynska, K. D., Zielinska, D., Wozna-Wysocka, M., Krzyzosiak, W. J., Fiszer, A.
    Academic press:
    Cellular and Molecular Life Sciences (year: 2021, volume: 78, pages: 1577-1596), Publisher: Springer Science+Business Media
    Status:
    Published
    DOI:
    10.1007/s00018-020-03596-7 – link to the publication

  5. Generation of New Isogenic Models of Huntington's Disease Using CRISPR-Cas9 Technology

    Authors:
    Dabrowska, M., Ciolak, A., Kozlowska, E., Fiszer, A., Olejniczak, M.
    Academic press:
    International Journal of Molecular Sciences (year: 2020, volume: 21, pages: 1854), Publisher: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms21051854 – link to the publication

  6. Generation of human iPS cell line IBCHi002-A from spinocerebellar ataxia type 3/Machado-Joseph disease patient's fibroblasts

    Authors:
    Ciolak, A., Krzyzosiak, W. J., Kozlowska, E., Fiszer, A.
    Academic press:
    Stem Cell Research (year: 2020, volume: 45, pages: 101796), Publisher: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.scr.2020.101796 – link to the publication

  7. Reduction of Huntington's Disease RNA Foci by CAG Repeat-Targeting Reagents

    Authors:
    Urbanek M.O., Fiszer A., Krzyzosiak W.J.
    Academic press:
    Frontiers in Cellular Neuroscience (year: 2017, volume: 11, pages: 82), Publisher: Frontiers Media SA
    Status:
    Published
    DOI:
    10.3389/fncel.2017.00082 – link to the publication

  8. Identifying proteins that bind to specific RNAs - focus on simple repeat expansion diseases

    Authors:
    Jazurek M., Ciesiolka A., Starega-Roslan J., Bilinska K., Krzyzosiak W.J.
    Academic press:
    Nucleic Acids Research (year: 2016, volume: 44, pages: 9050 – 9070), Publisher: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/nar/gkw803 – link to the publication

  9. Nuclear speckles: molecular organization, biological function and role in Disease

    Authors:
    Galganski L., Urbanek M.O., Krzyzosiak W.J.
    Academic press:
    Nucleic Acids Research (year: 2017, volume: 45, pages: 10350-10368), Publisher: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/nar/gkx759 – link to the publication

  10. Structural Characteristics of Simple RNA Repeats Associated with Disease and their Deleterious Protein Interactions

    Authors:
    Ciesiolka A., Jazurek M., Drazkowska K., Krzyzosiak W.J.
    Academic press:
    Frontiers in Cellular Neuroscience (year: 2017, volume: 11, pages: 97), Publisher: Frontiers Media SA
    Status:
    Published
    DOI:
    10.3389/fncel.2017.00097 – link to the publication

  11. 2D and 3D FISH of expanded repeat RNAs in human lymphoblasts

    Authors:
    Urbanek M.O., Michalak M., Krzyzosiak W.J.
    Academic press:
    Methods (year: 2017, volume: 120, pages: 49-57), Publisher: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.ymeth.2017.04.002 – link to the publication

  12. Discriminating RNA variants with single-molecule allele-specific FISH

    Authors:
    Urbanek M.O., Krzyzosiak W.J.
    Academic press:
    Mutation Research – Reviews in Mutation Research (year: 2017, volume: 773, pages: 230-241), Publisher: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.mrrev.2016.09.003 – link to the publication

Articles in post-conference publications (1)

  1. Ribosome meets RISC at expanded CAG repeat tract - allele-selective RNAi approach for therapy of polyglutamine diseases

    Authors:
    P. Joachimiak, A. Ciesiolka, A. Luzna, E. Kozlowska, M. Michalak, W.J. Krzyzosiak, A. Fiszer
    Conference:
    The 44th FEBS Congress: From molecules to living systems (year: 2019, pages: 50-51), Publisher: John Wiley & Sons on behalf of the Federation of European Biochemical Societies
    Date:
    conference 43622
    Status:
    Published

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