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Products of DNA demethylation and deamination pathways as new biomarkers of development and predictors of the outcome in acute leukemias.

2015/19/B/NZ5/02208

Keywords

Descriptors

  • NZ5_3: Patogeneza chorób człowieka
  • NZ5_5: Diagnostyka chorób człowieka
  • NZ1_2: Biochemia

Panel

NZ5 – Human and animal noninfectious diseases: etiology, mechanisms, diagnosis and treatment of diseases, poisonings and injuries (without neurological diseases)

Host institution

Uniwersytet Mikołaja Kopernika w Toruniu, Collegium Medicum im. Ludwika Rydygiera w Bydgoszczy; Wydział Farmaceutyczny

Voivodeship: kujawsko-pomorskie

Other projects carried out by the institution »

Principal investigator (from the host institution)

dr hab. Daniel Stefan Gackowski

Number of co-investigators in the project: 16

Call

OPUS 10 – announced on 2015-09-15

Amount awarded
1 575 920 PLN
Project start date
2016-07-11
Project end date
2021-07-10
Project duration
60 months (the same as in the proposal)
Project status
Project settled

Project description

Download the project description in a pdf file

Note — project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. Aparat do izolacji DNA. Cost: 120 000 PLN
  2. Komputery stacjonarne i przenośne z oprogramowaniem (2 pcs). Cost: 12 000 PLN
  3. Magnetyczny separator komórek. Cost: 5 912 PLN

Information in the final report

Publication in academic press/journals (5)

  1. Diagnostic and prognostic power of active DNA demethylation pathway intermediates in acute myelogenous leukemia and myelodysplastic syndromes

    Authors:
    Aleksandra Skalska-Bugala, Marta Starczak, Łukasz Szukalski, Maciej Gawronski, Agnieszka Siomek-Gorecka, Justyna Szpotan, Anna Labejszo, Ewelina Zarakowska1, Anna Szpila, Anna Jachalska, Adriana Szukalska, Marcin Kruszewski, Anna Sadowska, Aleksandra Wasilow, Patrycja Baginska, Jaroslaw Czyz, Ryszard Olinski, Rafal Rozalski, Daniel Gackowski
    Academic press:
    Cells (year: 2022, volume: 11, pages: 888), Publisher: Multidisciplinary Digital Publishing Institute
    Status:
    Published
    DOI:
    10.3390/cells11050888 – link to the publication

  2. Profiles of a broad spectrum of epigenetic DNA modifications in normal and malignant human cell lines: Proliferation rate is not the major factor responsible for the 5- hydroxymethyl-20-deoxycytidine level in cultured cancerous cell lines

    Authors:
    Marek Foksinski, Ewelina Zarakowska, Daniel Gackowski, Magdalena Skonieczna, Karolina Gajda, Dorota Hudy, Anna Szpila, Karol Bialkowski, Marta Starczak, Anna Labejszo, Jaroslaw Czyz, Joanna Rzeszowska-Wolny, Ryszard Olinski
    Academic press:
    PLOS ONE (year: 2017, volume: 12(11), pages: e0188856), Publisher: Public Library of Science
    Status:
    Published
    DOI:
    10.1371/journal.pone.0188856 – link to the publication

  3. 5-Formylcytosine and 5-hydroxymethyluracil as surrogate markers of TET2 and SF3B1 mutations in myelodysplastic syndrome, respectively

    Authors:
    Daniel Gackowski, Maciej Gawroński, C. Kerr, T. Radivoyevitch, Ewelina Zarakowska, Marta Starczak, Abdulkadir Abakir, Alexey Ruzow, Jaroslaw P. Maciejewski, Ryszard Olińsk
    Academic press:
    Haematologica (year: 2020, volume: 105 (5), pages: 46026), Publisher: Ferrata Storti Foundation
    Status:
    Published
    DOI:
    10.3324/haematol.2019.224030 – link to the publication

  4. The urinary excretion of epigenetically modified DNA as a marker of pediatric ALL status and chemotherapy response

    Authors:
    Rafal Rozalski, Daniel Gackowski, Aleksandra Skalska-Bugała, Marta Starczak, Agnieszka Siomek-Gorecka, Ewelina Zarakowska, Martyna Modrzejewska, Tomasz Dziaman, Anna Szpila, Kinga Linowiecka, Jolanta Guz1, Justyna Szpotan, Maciej Gawronski, Anna Labejszo, Lidia Gackowska, Marek Foksinski, Elwira Olinska,, Aleksandra Wasilow, Andrzej Koltan, Jan Styczynski & Ryszard Olinski
    Academic press:
    Scientific Reports (year: 2021, volume: 11, pages: 21345), Publisher: Springer Nature
    Status:
    Published
    DOI:
    10.1038/s41598-021-00880-9 – link to the publication

  5. Context dependent effects of ascorbic acid treatment in TET2 mutant myeloid neoplasia

    Authors:
    Yihong Guan, Edward F. Greenberg, Metis Hasipek, Shi Chen, Xiaochen Liu, Cassandra M. Kerr, Daniel Gackowski, Ewelina Zarakowska, Tomas Radivoyevitch, Xiaorong Gu, Belinda Willard, Valeria Visconte, Hideki Makishima, Aziz Nazha, Mridul Mukherji, Mikkael A. Sekeres, Yogen Saunthararajah, Ryszard Oliński, Mingjiang Xu, Jaroslaw P. Maciejewski & Babal K. Jha
    Academic press:
    Communications Biology (year: 2020, volume: 3, pages: 493), Publisher: Springer Nature
    Status:
    Published
    DOI:
    10.1038/s42003-020-01220-9 – link to the publication

Articles in post-conference publications (7)

  1. The enzymes involved in active DNA demethylation as a potential biomarkers in B-cell acute lymphoblastic leukemia and chronic lymphocytic leukemia.

    Authors:
    Anna Łabejszo, Lidia Gackowska, Anna Helmin-Basa, Izabela Kubiszewska, Andrzej Kołtan, Jan Styczyński, Jarosław Czyż, Daniel Gackowski.
    Conference:
    ESCCA 2018: At the shore of future cytometry. (year: 2018, pages: Suppl. mat. page 15 (abstract number: ADV-01)), Publisher: Cytometry Part B (Clinical Cytometry); ESCCA and John Wiley & Sons
    Date:
    conference 13-15 September 2018
    Status:
    Published

  2. Relationship between the levels of epigenetic DNA modifications, 2-oxoglutarate and 2-hydroxyglutarates in acute leukemias.

    Authors:
    Justyna Szpotan, Martyna Modrzejewska, Kinga Linowiecka, Ewelina Zarakowska, Anna Szpila, Agnieszka Siomek-Górecka, Marta Starczak, Maciej Gawroński, Anna Łabejszo, Marek Foksiński, Jarosław Czyż, Andrzej Kołtan, Jan Styczyński, G. Charliński, Daniel Gackowski.
    Conference:
    43rd FEBS Congress Biochemistry forever 2018 (year: 2018, pages: 338 (abstract number: P.10-136-Mon)), Publisher: FEBS Open Bio (FEBS Press and John Wiley & Sons)
    Date:
    conference 7-12 July 2018
    Status:
    Published

  3. Assessment of pathway of active DNA demethylation in myelodysplastic syndrome

    Authors:
    Kinga Linowiecka, Jolanta Guz, Tomasz Dziaman, Justyna Szpotan, Martyna Modrzejewska, Marta Starczak, Maciej Gawroński, Anna Łabejszo, Marek Foksiński, Jarosław Czyż, Andrzej Kołtan, Jan Styczyński, G. Charliński, Daniel Gackowski.
    Conference:
    43rd FEBS Congress Biochemistry forever 2018 (year: 2018, pages: 335 (abstract number: P.10-127-Mon)), Publisher: FEBS Open Bio (FEBS Press and John Wiley & Sons)
    Date:
    conference 7-12 July 2018
    Status:
    Published

  4. Assessment of active DNA demethylation pathway during chemotherapy of acute myeloid leukemia

    Authors:
    Anna Łabejszo, Marta Starczak, Lidia Gackowska, Martyna Modrzejewska, Justyna Szpotan, Maciej Gawroński, Andrzej Kołtan, G. Charliński, Jarosław Czyż, Jan Styczyński, Daniel Gackowski.
    Conference:
    43rd FEBS Congress Biochemistry forever 2018 (year: 2018, pages: 282 (abstract number: P.09-233-Tue)), Publisher: FEBS Open Bio (FEBS Press and John Wiley & Sons)
    Date:
    conference 7-12 July 2018
    Status:
    Published

  5. The Assessment of Expression of TETs and AID Enzymes Involved in Active DNA Demethylation, in Patients with B-Cell Acute Lymphoblastic Leukemia

    Authors:
    Lidia Gackowska, Anna Labejszo, Anna Helmin- Basa, Andrzej Koltan, Jan Styczynski, Daniel Gackowski
    Conference:
    33rd Congress of the International Society for Advancement of Cytometry. CYTO 2018 (year: 2018, pages: 280 (abstract number B244)), Publisher: International Society for Advancement of Cytometry (ISAC)
    Date:
    conference April 28-May 2 2018
    Status:
    Published

  6. Vitamin C restores TET proteins activity in leukemic cells.

    Authors:
    Maciej Gawroński, Marta Starczak, Anna Łabejszo, Martyna Modrzejewska, Daniel Gackowski.
    Conference:
    43rd FEBS Congress Biochemistry forever 2018 (year: 2018, pages: 108 (abstract number: P.01-005-Tue)), Publisher: FEBS Open Bio (FEBS Press and John Wiley & Sons)
    Date:
    conference 7-12 July 2018
    Status:
    Published

  7. Development and validation of indirect flow cytometry method for analysis of expression of proteins involved in active DNA demethylation in patients with acute leukemia.

    Authors:
    Anna Łabejszo, Lidia Gackowska, Maciej Gawroński, Marta Starczak, Daniel Gackowski.
    Conference:
    33rd Congress of the International Society for Advancement of Cytometry. CYTO 2018 (year: 2018, pages: 204 (abstract number B245)), Publisher: International Society for Advancement of Cytometry (ISAC)
    Date:
    conference April 28-May 2 2018
    Status:
    Published

Book publications / chapters in book publications (3)

  1. Quantification of DNA modifications using two-dimensional ultraperformance liquid chromatography tendem mass spectrometry (2D-UPLC-MS/MS).

    Authors:
    Marta Starczak, Maciej Gawronski, Ryszard Olinski, and Daniel Gackowski
    Book:
    Methods in molecular biology: DNA modifications: methods and protocols. (year: 2021, volume: 2198, pages: 91-108), Publisher: Humana Press, Springer US
    Status:
    Published

  2. Preparation of internal standards for 2D-UPLC-MS/MS quantification of noncanonical DNA bases

    Authors:
    Starczak Marta, Skalska Aleksandra, Różalski Rafał, Oliński Ryszard, Gackowski Daniel
    Book:
    Methods in molecular biology: DNA modifications: methods and protocols. (year: 2021, volume: 2198, pages: 123-136), Publisher: Humana Press, Springer US
    Status:
    Published

  3. MS analysis of DNA modifications in urinary/body fluids.

    Authors:
    Skalska Aleksandra, Siomek-Górecka Agnieszka, Oliński Ryszard, Różalski Rafał
    Book:
    Methods in molecular biology: DNA modifications: methods and protocols. (year: 2021, volume: 2198, pages: 109-122), Publisher: Humana Press, Springer US
    Status:
    Published

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Graphic design and implementation: Jakub Piotrowski