Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

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Transcriptome analysis of boar spermatozoa and its association with semen quality following cryopreservation

2015/19/B/NZ9/01333

Keywords:

spermatozoa boar cryopreservation transcriptome RNA-seq

Descriptors:

  • NZ9_2: Animal production

Panel:

NZ9 - Fundamentals of applied life sciences and biotechnology: agricultural, forestry, horticulture, animal production and fishery, food and nutrition sciences, industrial biosciences, environmental biotechnology and remediation

Host institution :

Uniwersytet Warmińsko-Mazurski w Olsztynie, Wydział Bioinżynierii Zwierząt

woj. warmińsko-mazurskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Leyland Fraser 

Number of co-investigators in the project: 4

Call: OPUS 10 - announced on 2015-09-15

Amount awarded: 538 200 PLN

Project start date (Y-m-d): 2016-06-15

Project end date (Y-m-d): 2020-06-14

Project duration:: 48 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Information in the final report

  • Publication in academic press/journals (7)
  • Articles in post-conference publications (12)
  • Book publications / chapters in book publications (1)
  1. Gene promoter polymorphisms in boar spermatozoa differing in freezability
    Authors:
    Paweł Brym, Karolina Wasilewska-Sakowska, Marzena Mogielnicka-Brzozowska, Anna Mańkowska, Łukasz Paukszto, Chandra S. Pareek, Władysław Kordan, Stanisław Kondracki, Leyland Fraser
    Academic press:
    Theriogenology (rok: 2021, tom: 166, strony: 112-123), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.theriogenology.2021.02.018 - link to the publication
  2. Isolation of total ribonucleic acid from fresh and frozen-thawed boar semen and its relevance in transcriptome studies
    Authors:
    Leyland Fraser, Paweł Brym, Chandra S. Pareek
    Academic press:
    South African Journal of Animal Science (rok: 2017, tom: 47, strony: 56-60), Wydawca: South African Society for Animal Science
    Status:
    Published
    DOI:
    10.4314/sajas.v47i1.9 - link to the publication
  3. Regulatory potential of long non-coding RNAs (lncRNAs) in boar spermatozoa with good and poor freezability
    Authors:
    Leyland Fraser, Łukasz Paukszto, Anna Mańkowska, Paweł Brym, Przemysław Gilun, Jan P. Jastrzębski, Chandra S. Pareek, Dibyendu Kumar, Mariusz Pierzchała
    Academic press:
    Life (rok: 2020, tom: 10, strony: 300), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/life10110300 - link to the publication
  4. Sperm DNA damage in relation to lipid peroxidation following freezing-thawing of boar semen
    Authors:
    Leyland Fraser, Jerzy Strzeżek, Karolina Wasilewska, Chandra S. Pareek
    Academic press:
    South African Journal of Animal Science (rok: 2017, tom: 47, strony: 213-218), Wydawca: South African Society for Animal Science
    Status:
    Published
    DOI:
    10.4314/sajas.v47i2.13 - link to the publication
  5. Total RNA quality in boar spermatozoa with different freezability
    Authors:
    Leyland Fraser, Paweł Brym, Marzena Mogielnicka-Brzozowska, Karolina Wasilewska
    Academic press:
    Polish Journal of Veterinary Sciences (rok: 2019, tom: 20, strony: 181-185), Wydawca: Polish Academy of Sciences Committee of Veterinary Sciences, University of Warmia and Mazury in Olsztyn
    Status:
    Published
    DOI:
    10.24425/pjvs.2019.127085 - link to the publication
  6. Gene polymorphisms in boar spermatozoa and their associations with post-thaw semen quality
    Authors:
    Anna Mańkowska, Paweł Brym, Łukasz Paukszto , Jan P. Jastrzębski, Leyland Fraser
    Academic press:
    International Journal of Molecular Sciences (rok: 2020, tom: 21, strony: 1902), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms21051902 - link to the publication
  7. Transcriptome analysis of boar spermatozoa with different freezability using RNA-Seq
    Authors:
    Leyland Fraser, Paweł Brym, Chandra S. Pareek, Marzena Mogielnicka-Brzozowska, Łukasz Paukszto, Jan P. Jastrzębski, Karolina Wasilewska-Sakowska, Anna Mańkowska, Przemysław Sobiech, Kacper Żukowski
    Academic press:
    Theriogenology (rok: 2020, tom: 142, strony: 400-413), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.theriogenology.2019.11.001 - link to the publication
  1. Quality of total RNA isolated from spermatozoa of boars with different semen freezability
    Authors:
    Leyland Fraser, Chandra S. Pareek, Karolina Wasilewska, Marzena Mogielnicka-Brzozowska
    Conference:
    Reproduction in Domestic Animals, Proceedings of the 21st Annual Conference of the European Society for Domestic Animal Reproduction (ESDAR); Publikacja indeksowana w bazie Web of Science (rok: 2017, ), Wydawca: Wiley Blackwell
    Data:
    konferencja 24-26 sierpnia
    Status:
    Published
  2. Variations in expression of boar sperm transcripts analyzed by RNA-Seq
    Authors:
    Karolina Wasilewska-Sakowska, Leyland Fraser, Paweł Brym, Anna Mańkowska
    Conference:
    Proceedings of the 23rd Annual Conference of the European Society for Domestic Animal Reproduction (ESDAR), Reproduction in Domestic Animals (rok: 2019, ), Wydawca: Wiley Blackwell
    Data:
    konferencja 19-22 września
    Status:
    Published
  3. A preliminary study on transcriptome analysis of boar spermatozoa differed in freezability
    Authors:
    Leyland Fraser, Chandra S. Pareek, Paweł Brym, Karolina Wasilewska, Min Tu,Yaping Feng, Dibyendu Kumar
    Conference:
    Proceedings of the 22nd Annual Conference on the European Society for Domestic Animal Reproduction (ESDAR) (rok: 2018, ), Wydawca: Wiley Blackwell
    Data:
    konferencja 27-29th September
    Status:
    Published
  4. Gene expression profiling of boar spermatozoa with differences in freezability
    Authors:
    Chandra S. Pareek, Leyland Fraser
    Conference:
    18th Annual Conference in Bioinformatics (18th BioInformatics – BIT18) (rok: 2018, ), Wydawca: Polskie Towarzystwo Bioinformatyczne
    Data:
    konferencja 28-30th June
    Status:
    Published
  5. Motion characteristics and membrane integrity of boar spermatozoa differing in freezability
    Authors:
    Leyland Fraser, Łukasz Zasiadczyk, Karolina Wasilewska, Krystyna Filipowicz, Krzysztof Karpiesiuk
    Conference:
    Program and Streszczenia, VIII Zjazd Towarzystwa Biologii Rozrodu (rok: 2017, ), Wydawca: Towarzystwa Biologii Rozrodu, Olsztyn
    Data:
    konferencja 7-9 września
    Status:
    Published
  6. Profile białek błonowych plemników knura o dobrej i słabej przydatności do kriokonserwacji (ang. Membrane protein profiles of boar spermatozoa with good and poor freezability).
    Authors:
    Anna Mańkowska, Leyland Fraser
    Conference:
    Ogólnopolska Konferencja Naukowa KrakWet"– postępy w naukach weterynaryjnych i biotechnologii zwierząt (rok: 2020, ), Wydawca: Fundacja na rzecz promocji nauki i rozwoju TYGIEL
    Data:
    konferencja 43888
    Status:
    Published
  7. Big data analysis of porcine spermatozoa transcriptome by RNA-seq
    Authors:
    Chandra S. Pareek, Leyland Fraser
    Conference:
    Proceedings of the 17th Annual Conference in Bioinformatics (17th BioInformatics – BIT17) (rok: 2017, ), Wydawca: Polskie Towarzystwo Bioinformatyczne (PTBI), Toruń
    Data:
    konferencja 22-24 czerwca
    Status:
    Published
  8. Post-thaw characteristics of boar spermatozoa with different freezability
    Authors:
    Leyland Fraser, Karolina Wasilewska, Łukasz Zasiadczyk
    Conference:
    Research in pig breeding : Workshop Proceedings of Abstracts (rok: 2017, ), Wydawca: Institue of Animal Science Prague Uhrineves, Department of Pig Breeding , Czech Academy of Agricultural Sciences
    Data:
    konferencja 17-18 października
    Status:
    Published
  9. An improved protocol for total RNA extraction and QC of boar spermatozoa for RNA-Seq studies
    Authors:
    Paweł Brym, Karolina Wasilewska, Leyland Fraser
    Conference:
    Conference on Animal Genetics and Genomics (rok: 2018, ), Wydawca: University of Warmia and Mazury in Olsztyn; Polish Society of Genetics (Olsztyn Division)
    Data:
    konferencja 2-4th July
    Status:
    Published
  10. Analysis of gene transcript expression in boar spermatozoa differed in freezability
    Authors:
    Leyland Fraser, Anna Mańkowska, Paweł Brym, Marzena Mogielnicka-Brzozowska
    Conference:
    The 5th Winter Workshop of the Society for Biology of Reproduction "Central and Local Regulations of Reproductive Processes" (rok: 2019, ), Wydawca: Krakowski Oddział Towarzystwa Biologii Rozrodu
    Data:
    konferencja 13-15 lutego 2019
    Status:
    Published
  11. Differentially expressed gene transcripts in boar spermatozoa analyzed by RNA-Seq
    Authors:
    Leyland Fraser,Paweł Brym, Karolina Wasilewska
    Conference:
    51st Meeting of the Polish Biochemical Society, 14th Conference of the Polish Society for Cell Biology (Congress BIO2018) (rok: 2018, ), Wydawca: Polish Biochemical Society, Polish Society for Cell Biology
    Data:
    konferencja 18-21st September
    Status:
    Published
  12. Gene expression profiles of boar spermatozoa differed in semen freezability
    Authors:
    Leyland Fraser, Paweł Brym, Karolina Wasilewska, Łukasz Paukszto, Jan P. Jastrzębski, Chandra S. Pareek
    Conference:
    Conference on Animal Genetics and Genomics (rok: 2018, ), Wydawca: University of Warmia and Mazury in Olsztyn; Polish Society of Genetics (Olsztyn Division)
    Data:
    konferencja 2-4th July
    Status:
    Published
  1. Markers for Sperm Freezability and Relevance of Transcriptome Studies in Semen Cryopreservation: A Review
    Authors:
    Leyland Fraser
    Book:
    Theriogenology, Biochemistry, Genetics and Molecular Biology (rok: 2017, ), Wydawca: InTech Europe
    Status:
    Published

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