Projects funded by the NCN


Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Mapping positive and negative memories in the brain

2015/17/B/NZ4/02631

Keywords:

learning brain mapping whole-brain imaging light-sheet microscopy

Descriptors:

  • NZ4_9: Systems neurobiology
  • NZ4_1: Anatomy

Panel:

NZ4 - Biology of tissues, organs and organisms: morphology and functions of animal's and human's systems, organs and organisms, experimental medicine, basics of neurology

Host institution :

Instytut Biologii Doświadczalnej im. M. Nenckiego PAN

woj. mazowieckie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr Monika Pawłowska 

Number of co-investigators in the project: 3

Call: OPUS 9 - announced on 2015-03-16

Amount awarded: 844 800 PLN

Project start date (Y-m-d): 2016-02-22

Project end date (Y-m-d): 2020-02-21

Project duration:: 48 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. obiektyw długozasięgowy do imersji w tkance wraz z wyposażeniem (160 000 PLN)
  2. serwer przechowywania danych NAS wraz z dyskami 72TB (17 622 PLN)
  3. jednostka laserowa z zasilaczem, sprzęgaczem wiązek i dwoma liniami laserowymi (80 000 PLN)

Information in the final report

  • Publication in academic press/journals (4)
  • Book publications / chapters in book publications (1)
  1. Zajrzeć w głąb mózgu – nowe techniki oczyszczania optycznego i obrazowania z zastosowaniem mikroskopu arkusza światła
    Authors:
    Monika Pawłowska, Diana Legutko, Marzena Stefaniuk
    Academic press:
    Postępy Biochemii (rok: 2017, tom: 63, strony: 45519), Wydawca: Polskie Towarzystwo Biochemiczne
    Status:
    Published
  2. Optimized perfusion-based CUBIC protocol for the efficient whole-body clearing and imaging of rat organs
    Authors:
    Matryba Paweł, Bozycki Lukasz, Pawłowska Monika, Kaczmarek Leszek, Stefaniuk Marzena
    Academic press:
    Journal of Biophotonics (rok: 2018, tom: 11, strony: e201700248), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/jbio.201700248 - link to the publication
  3. Light-sheet microscopy imaging of a whole cleared rat brain with Thy1-GFP transgene
    Authors:
    Marzena Stefaniuk, Emilio J Gualda, Monika Pawlowska, Diana Legutko, Paweł Matryba, Paulina Koza, Witold Konopka, Dorota Owczarek, Marcin Wawrzyniak, Pablo Loza-Alvarez, Leszek Kaczmarek
    Academic press:
    Scientific Reports (rok: 2016, tom: 6, strony: 28209), Wydawca: Nature Publishing Group
    Status:
    Published
    DOI:
    10.1038/srep28209 - link to the publication
  4. Tissue clearing-based method for unobstructed three-dimensional imaging of mouse penis with subcellular resolution
    Authors:
    Paweł Matryba, Artur Wolny, Monika Pawłowska, Anna Sosnowska, Zuzanna Rydzyńska, Marcin Jasiński, Marzena Stefaniuk, Jakub Gołąb
    Academic press:
    Journal of Biophotonics (rok: 2020, tom: 13, strony: e202000072), Wydawca: Wiley-VCH
    Status:
    Published
    DOI:
    10.1002/jbio.202000072 - link to the publication
  1. Light-Sheet Microscopy for Whole-Brain Imaging
    Authors:
    M Pawłowska, M Stefaniuk, D Legutko, L Kaczmarek
    Book:
    Advanced Optical Methods for Brain Imaging (rok: 2019, tom: 1, strony: 69-81), Wydawca: Springer Singapore
    Status:
    Published