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Biosynthesis of seleno-modified nucleosides in the anticodon loop of transfer ribonucleic acids. Evaluation of the enzymatic transformation of 2-thiouridine to 2-selenouridine, studies on function and structure of E. coli tRNA selenouridine synthase.

2014/13/B/ST5/03979

Keywords

Descriptors

  • ST5_16: Chemia biologiczna
  • ST5_23: Chemia organiczna
  • ST5_25: Inne zagadnienia pokrewne

Panel

ST5 – Materials: materials synthesis, structure-properties relations, advanced and functional materials with designed properties, (macro)molecular architecture, material engineering

Host institution

Centrum Badań Molekularnych i Makromolekularnych PAN

Voivodeship: łódzkie

Other projects carried out by the institution »

Principal investigator (from the host institution)

prof. Barbara Czesława Nawrot

Number of co-investigators in the project: 11

Call

OPUS 7 – announced on 2014-03-17

Amount awarded
1 252 500 PLN
Project start date
2015-04-01
Project end date
2019-03-31
Project duration
48 months (the same as in the proposal)
Project status
Project settled

Equipment purchased [PL]

  1. Rozdzielacz przepływu. Cost: 6 000 PLN
  2. Aparat do termoforezy. Cost: 475 000 PLN
  3. Dwustopniowa pompa rotacyjna (ciśnienie w zakresie 10-3 torr). Cost: 8 000 PLN
  4. Pompa próżniowa, membranowa. Cost: 5 500 PLN
  5. Reodyna. Cost: 6 500 PLN
  6. Semi-preparatywna/analityczna kolumna anionowymienna HPLC. Cost: 4 000 PLN

Information in the final report

Publication in academic press/journals (8)

  1. C5-Substituted 2-Selenouridines Ensure Efficient Base Pairing with Guanosine; Consequences for Reading the NNG-3′ Synonymous mRNA Codons

    Authors:
    Grazyna Leszczynska , Marek Cypryk , Bartlomiej Gostynski2, Klaudia Sadowska , Paulina Herman , Grzegorz Bujacz , Elzbieta Lodyga-Chruscinska , Elzbieta Sochacka and Barbara Nawrot ,*
    Academic press:
    Int. J. Mol. Sci (year: 2020, volume: 21, pages: 2882), Publisher: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms21082882 – link to the publication

  2. Reaction of S-geranyl-2-thiouracil modified oligonucleotides with alkyl amines leads to the N2-alkyl isocytosine derivatives.

    Authors:
    Leszczynska G., Sadowska K., Sierant M., Sobczak M., Nawrot B., Sochacka E.
    Academic press:
    Org. Biomol. Chem. (year: 2017, volume: 15, pages: 5332-5336), Publisher: Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/c7ob01012e – link to the publication

  3. Cytochrome C catalyzes the hydrogen peroxide-assisted oxidative desulfuration of 2-thiouridines in transfer RNAs.

    Authors:
    Sierant, M., Kulik, K., Sochacka, E., Szewczyk, R., Sobczak, M. and Nawrot, B.
    Academic press:
    ChemBioChem (year: 2018, volume: 19, pages: 687-695), Publisher: Wiley
    Status:
    Published
    DOI:
    10.1002/cbic.201700692 – link to the publication

  4. Escherichia coli tRNA 2-selenouridine synthase (SelU) converts S2U-RNA to Se2U-RNA via S-geranylated-intermediate.

    Authors:
    Sierant M, Leszczynska G, Sadowska K, Komar P, Radzikowska-Cieciura E, Sochacka E, Nawrot B.
    Academic press:
    FEBS Lett (year: 2018, volume: 592 (13), pages: 2248-2258), Publisher: Wiley
    Status:
    Published
    DOI:
    10.1002/1873-3468.13124 – link to the publication

  5. S-Geranylated 2-Thiouridines of Bacterial tRNAs: Chemical Synthesis and Physicochemical Properties

    Authors:
    G. Leszczynska, K. Sadowska, P. Bartos, B. Nawrot, E. Sochacka
    Academic press:
    Eur. J. Org. Chem. (year: 2016, volume: 2016, pages: 3482-3485), Publisher: Wiley
    Status:
    Published
    DOI:
    10.1002/ejoc.201600519 – link to the publication

  6. Nucleoside modifications in the regulation of gene expression: focus on tRNA

    Authors:
    M. Düchler, G. Leszczyńska, E. Sochacka, B. Nawrot
    Academic press:
    Cell Mol Life Sci (year: 2016, volume: 73, pages: 3075-3095), Publisher: Springer
    Status:
    Published
    DOI:
    link to the publication

  7. S-Geranyl-2-thiouridine wobble nucleosides of bacterial tRNAs; chemical and enzymatic synthesis of S-geranylated-RNAs and their physicochemical characterization

    Authors:
    M. Sierant, G. Leszczynska, K. Sadowska, A. Dziergowska, M. Rozanski, E. Sochacka. B. Nawrot
    Academic press:
    Nucleic Acids Res (year: 2016, volume: 44(22), pages: 10986-10996), Publisher: Oxford Academic
    Status:
    Published
    DOI:
    10.1093/nar/gkw727 – link to the publication

  8. C5-substituents of uridines and 2-thiouridines present at the wobble position of tRNA determine the formation of their keto-enol or zwitterionic forms - a factor important for accuracy of reading of guanosine at the 3΄-end of the mRNA codons.

    Authors:
    Sochacka E, Lodyga-Chruscinska E, Pawlak J, Cypryk M, Bartos P, Ebenryter-Olbinska K, Leszczynska G, Nawrot B.
    Academic press:
    Nucleic Acids Res. (year: 2017, volume: 45, pages: 4825-4836), Publisher: Oxford Academic
    Status:
    Published
    DOI:
    10.1093/nar/gkw1347 – link to the publication

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