Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

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Biosynthesis of seleno-modified nucleosides in the anticodon loop of transfer ribonucleic acids. Evaluation of the enzymatic transformation of 2-thiouridine to 2-selenouridine, studies on function and structure of E. coli tRNA selenouridine synthase.

2014/13/B/ST5/03979

Keywords:

biosynthesis of modified nucleosides anticodon tRNA 2-thiouridine 2-selenouridine selenouridine synthase crystallography thermophoresis

Descriptors:

  • ST5_16:
  • ST5_23:
  • ST5_25:

Panel:

ST5 - Materials: materials synthesis, structure-properties relations, advanced and functional materials with designed properties, (macro)molecular architecture, material engineering

Host institution :

Centrum Badań Molekularnych i Makromolekularnych Polskiej Akademii Nauk

woj. łódzkie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Barbara Czesława Nawrot 

Number of co-investigators in the project: 11

Call: OPUS 7 - announced on 2014-03-17

Amount awarded: 1 252 500 PLN

Project start date (Y-m-d): 2015-04-01

Project end date (Y-m-d): 2019-03-31

Project duration:: 48 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Aparat do termoforezy (475 000 PLN)
  2. Reodyna (6 500 PLN)
  3. Semi-preparatywna/analityczna kolumna anionowymienna HPLC (4 000 PLN)
  4. Rozdzielacz przepływu (6 000 PLN)
  5. Dwustopniowa pompa rotacyjna (ciśnienie w zakresie 10-3 torr) (8 000 PLN)
  6. Pompa próżniowa, membranowa (5 500 PLN)

Information in the final report

  • Publication in academic press/journals (16)
  1. S-Geranyl-2-thiouridine wobble nucleosides of bacterial tRNAs; chemical and enzymatic synthesis of S-geranylated-RNAs and their physicochemical characterization
    Authors:
    M. Sierant, G. Leszczynska, K. Sadowska, A. Dziergowska, M. Rozanski, E. Sochacka. B. Nawrot
    Academic press:
    Nucleic Acids Res (rok: 2016, tom: 44(22), strony: 10986-10996), Wydawca: Oxford Academic
    Status:
    Published
    DOI:
    10.1093/nar/gkw727 - link to the publication
  2. Nucleoside modifications in the regulation of gene expression: focus on tRNA
    Authors:
    M. Düchler, G. Leszczyńska, E. Sochacka, B. Nawrot
    Academic press:
    Cell Mol Life Sci (rok: 2016, tom: 73, strony: 3075-3095), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007%2Fs00018-016-2217-y - link to the publication
  3. C5-Substituted 2-Selenouridines Ensure Efficient Base Pairing with Guanosine; Consequences for Reading the NNG-3′ Synonymous mRNA Codons
    Authors:
    Grazyna Leszczynska , Marek Cypryk , Bartlomiej Gostynski2, Klaudia Sadowska , Paulina Herman , Grzegorz Bujacz , Elzbieta Lodyga-Chruscinska , Elzbieta Sochacka and Barbara Nawrot ,*
    Academic press:
    Int. J. Mol. Sci (rok: 2020, tom: 21, strony: 2882), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms21082882 - link to the publication
  4. Reaction of S-geranyl-2-thiouracil modified oligonucleotides with alkyl amines leads to the N2-alkyl isocytosine derivatives.
    Authors:
    Leszczynska G., Sadowska K., Sierant M., Sobczak M., Nawrot B., Sochacka E.
    Academic press:
    Org. Biomol. Chem. (rok: 2017, tom: 15, strony: 5332-5336), Wydawca: Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/c7ob01012e - link to the publication
  5. Escherichia coli tRNA 2-selenouridine synthase (SelU) converts S2U-RNA to Se2U-RNA via S-geranylated-intermediate.
    Authors:
    Sierant M, Leszczynska G, Sadowska K, Komar P, Radzikowska-Cieciura E, Sochacka E, Nawrot B.
    Academic press:
    FEBS Lett (rok: 2018, tom: 592 (13), strony: 2248-2258), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/1873-3468.13124 - link to the publication
  6. Escherichia coli tRNA 2-selenouridine synthase (SelU) converts S2U-RNA to Se2U-RNA via S-geranylated-intermediate.
    Authors:
    Sierant M, Leszczynska G, Sadowska K, Komar P, Radzikowska-Cieciura E, Sochacka E, Nawrot B.
    Academic press:
    FEBS Lett (rok: 2018, tom: 592 (13), strony: 2248-2258), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/1873-3468.13124 - link to the publication
  7. C5-Substituted 2-Selenouridines Ensure Efficient Base Pairing with Guanosine; Consequences for Reading the NNG-3′ Synonymous mRNA Codons
    Authors:
    Grazyna Leszczynska , Marek Cypryk , Bartlomiej Gostynski2, Klaudia Sadowska , Paulina Herman , Grzegorz Bujacz , Elzbieta Lodyga-Chruscinska , Elzbieta Sochacka and Barbara Nawrot ,*
    Academic press:
    Int. J. Mol. Sci (rok: 2020, tom: 21, strony: 2882), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms21082882 - link to the publication
  8. C5-substituents of uridines and 2-thiouridines present at the wobble position of tRNA determine the formation of their keto-enol or zwitterionic forms - a factor important for accuracy of reading of guanosine at the 3΄-end of the mRNA codons.
    Authors:
    Sochacka E, Lodyga-Chruscinska E, Pawlak J, Cypryk M, Bartos P, Ebenryter-Olbinska K, Leszczynska G, Nawrot B.
    Academic press:
    Nucleic Acids Res. (rok: 2017, tom: 45, strony: 4825-4836), Wydawca: Oxford Academic
    Status:
    Published
    DOI:
    10.1093/nar/gkw1347 - link to the publication
  9. Nucleoside modifications in the regulation of gene expression: focus on tRNA
    Authors:
    M. Düchler, G. Leszczyńska, E. Sochacka, B. Nawrot
    Academic press:
    Cell Mol Life Sci (rok: 2016, tom: 73, strony: 3075-3095), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007%2Fs00018-016-2217-y - link to the publication
  10. S-Geranylated 2-Thiouridines of Bacterial tRNAs: Chemical Synthesis and Physicochemical Properties
    Authors:
    G. Leszczynska, K. Sadowska, P. Bartos, B. Nawrot, E. Sochacka
    Academic press:
    Eur. J. Org. Chem. (rok: 2016, tom: 2016, strony: 3482-3485), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/ejoc.201600519 - link to the publication
  11. Cytochrome C catalyzes the hydrogen peroxide-assisted oxidative desulfuration of 2-thiouridines in transfer RNAs.
    Authors:
    Sierant, M., Kulik, K., Sochacka, E., Szewczyk, R., Sobczak, M. and Nawrot, B.
    Academic press:
    ChemBioChem (rok: 2018, tom: 19, strony: 687-695), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/cbic.201700692 - link to the publication
  12. S-Geranylated 2-Thiouridines of Bacterial tRNAs: Chemical Synthesis and Physicochemical Properties
    Authors:
    G. Leszczynska, K. Sadowska, P. Bartos, B. Nawrot, E. Sochacka
    Academic press:
    Eur. J. Org. Chem. (rok: 2016, tom: 2016, strony: 3482-3485), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/ejoc.201600519 - link to the publication
  13. Reaction of S-geranyl-2-thiouracil modified oligonucleotides with alkyl amines leads to the N2-alkyl isocytosine derivatives.
    Authors:
    Leszczynska G., Sadowska K., Sierant M., Sobczak M., Nawrot B., Sochacka E.
    Academic press:
    Org. Biomol. Chem. (rok: 2017, tom: 15, strony: 5332-5336), Wydawca: Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/c7ob01012e - link to the publication
  14. C5-substituents of uridines and 2-thiouridines present at the wobble position of tRNA determine the formation of their keto-enol or zwitterionic forms - a factor important for accuracy of reading of guanosine at the 3΄-end of the mRNA codons.
    Authors:
    Sochacka E, Lodyga-Chruscinska E, Pawlak J, Cypryk M, Bartos P, Ebenryter-Olbinska K, Leszczynska G, Nawrot B.
    Academic press:
    Nucleic Acids Res. (rok: 2017, tom: 45, strony: 4825-4836), Wydawca: Oxford Academic
    Status:
    Published
    DOI:
    10.1093/nar/gkw1347 - link to the publication
  15. Cytochrome C catalyzes the hydrogen peroxide-assisted oxidative desulfuration of 2-thiouridines in transfer RNAs.
    Authors:
    Sierant, M., Kulik, K., Sochacka, E., Szewczyk, R., Sobczak, M. and Nawrot, B.
    Academic press:
    ChemBioChem (rok: 2018, tom: 19, strony: 687-695), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/cbic.201700692 - link to the publication
  16. S-Geranyl-2-thiouridine wobble nucleosides of bacterial tRNAs; chemical and enzymatic synthesis of S-geranylated-RNAs and their physicochemical characterization
    Authors:
    M. Sierant, G. Leszczynska, K. Sadowska, A. Dziergowska, M. Rozanski, E. Sochacka. B. Nawrot
    Academic press:
    Nucleic Acids Res (rok: 2016, tom: 44(22), strony: 10986-10996), Wydawca: Oxford Academic
    Status:
    Published
    DOI:
    10.1093/nar/gkw727 - link to the publication

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