Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Towards quantitative biology via novel method of mobility measurement in the living cell: interactions of proteins with intracellular structures of glycogen and mitochondria

2013/08/W/NZ1/00687

Keywords:

nano-viscosity diffusion interactions fluorescence correlation spectroscopy energy metabolism Drp1 GBE1 glycogen mitochondria

Descriptors:

  • NZ1_3: Biophysics
  • NZ3_1: Cell biology

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

Instytut Biologii Doświadczalnej im. M. Nenckiego PAN

woj. mazowieckie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Jerzy Duszyński 

Number of co-investigators in the project: 11

Call: SYMFONIA 1

Amount awarded: 5 000 000 PLN

Project start date (Y-m-d): 2013-12-09

Project end date (Y-m-d): 2018-07-08

Project duration:: 55 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Inkubator CO2 (z pokryciem komory wewnętrznej ze stopu miedzi) (60 000 PLN)
  2. licznik do komórek Countess II FL wraz z wyposażeniem.
  3. wyciągarka kapilar (35 000 PLN)
  4. autoklaw (10 000 PLN)
  5. demineralizator.
  6. kolumna do chromatografii wykluczenia (2 szt.) (20 000 PLN)
  7. pojemnik dewara do przechowywania zamrożonych komórek (15 000 PLN)
  8. mikroskop (100 000 PLN)
  9. wirówka z chłodzeniem (50 000 PLN)
  10. łaźnia wodna (5 000 PLN)
  11. aparat do mikrowstrzyknięć (stanowisko do mikrowstrzyknięć) (165 000 PLN)
  12. komora laminarna (50 000 PLN)
  13. pompka próżniowa z akcesoriami (10 000 PLN)
  14. termocykler.

Information in the final report

  • Publication in academic press/journals (14)
  • Articles in post-conference publications (4)
  1. Mechanizm fragmentacji mitochondriów – struktura i funkcja białka Drp1
    Authors:
    Bernadeta Michalska, Jerzy Duszyński, Jędrzej Szymański
    Academic press:
    Postępy Biochemii (rok: 2016, tom: 62, strony: 127-137), Wydawca: Polskie Towarzystwo Biochemiczne
    Status:
    Published
    DOI:
    brak - link to the publication
  2. Mitochondria and Reactive Oxygen Species in Aging and Age-Related Diseases
    Authors:
    Carlotta Giorgi, Saverio Marchi, Ines C.M. Simoes, Ziyu Ren, Giampaolo Morciano, Mariasole Perrone, Paulina Patalas-Krawczyk, Sabine Borchard, Paulina Jędrak, Karolina Pierzynowska, Jędrzej Szymański, David Q. Wang, Piero Portincasa, Grzegorz Węgrzyn, Hans Zischka, Pawel Dobrzyn, Massimo Bonora, Jerzy Duszynski, Alessandro Rimessi, Agnieszka Karkucinska-Wieckowska, Agnieszka Dobrzyn, Gyorgy Szabadkai, Barbara Zavan, Paulo J. Oliveira, Vilma A. Sardao, Paolo Pinton, Mariusz R. Wieckowski
    Academic press:
    International Review of Cell and Molecular Biology (rok: 2018, tom: 340, strony: 209-344), Wydawca: ScienceDirect
    Status:
    Published
    DOI:
    10.1016/bs.ircmb.2018.05.006 - link to the publication
  3. Motion of nanoprobes in complex liquids within the framework of the length-scale dependent viscosity model
    Authors:
    Tomasz Kalwarczyk, Krzysztof Sozanski, Anna Ochab-Marcinek, Jedrzej Szymanski, Marcin Tabaka, Sen Hou, Robert Holyst
    Academic press:
    Advances in Colloid and Interface Science (rok: 2015, tom: 223, strony: 55-63), Wydawca: ELSEVIER SCIENCE BV
    Status:
    Published
    DOI:
    10.1016/j.cis.2015.06.007 - link to the publication
  4. The effect of macromolecular crowding on mobility of biomolecules, association kinetics, and gene expression in living cells
    Authors:
    Tabaka Marcin, Kalwarczyk Tomasz, Szymański Jędrzej, Hou Sen, Hołyst Robert
    Academic press:
    Frontiers in Physics (rok: 2014, tom: 2, strony: 54), Wydawca: frontiers
    Status:
    Published
    DOI:
    10.3389/fphy.2014.00054 - link to the publication
  5. Towards improved precision in the quantification of surface-enhanced Raman scattering (SERS) enhancement factors: a renewed approach
    Authors:
    Arumugam Sivanesan, Witold Adamkiewicz, Govindasamy Kalaivani, Agnieszka Kaminska, Jacek Waluk, Robert Hołyst and Emad L. Izake
    Academic press:
    Analyst (rok: 2015, tom: 140, strony: 489-496), Wydawca: ROYAL SOC CHEMISTRY
    Status:
    Published
    DOI:
    10.1039/C4AN01778A - link to the publication
  6. Insight into the fission mechanism by quantitative characterization of Drp1 protein distribution in the living cell
    Authors:
    Michalska BM, Kwapiszewska K, Szczepanowska J, Kalwarczyk T, Patalas-Krawczyk P, Szczepański K, Hołyst R, Duszyński J, Szymański J.
    Academic press:
    Scientific Reports (rok: 2018, tom: 8, strony: 8122), Wydawca: NATURE PUBLISHING GROUP
    Status:
    Published
    DOI:
    10.1038/s41598-018-26578-z - link to the publication
  7. Bacteriophage-Based Bioconjugates as a Flow Cytometry Probe for Fast Bacteria Detection
    Authors:
    Marta Janczuk, Łukasz Richter,Grażyna Hoser,Jerzy Kawiak, Marcin Łoś,Joanna Niedziółka-Jonsson,Jan Paczesny, Robert Hołyst
    Academic press:
    BIOCONJUGATE CHEMISTRY (rok: 2017, tom: 28, strony: 419-425), Wydawca: AMER CHEMICAL SOC
    Status:
    Published
    DOI:
    10.1021/acs.bioconjchem.6b00596 - link to the publication
  8. Electrochemical pathway for the quantification of SERS enhancement factor
    Authors:
    Arumugam Sivanesan, Witold Adamkiewicz, Govindasamy Kalaivani, Agnieszka Kamińska, Jacek Waluk, Robert Hołyst, Emad L. Izake
    Academic press:
    Electrochemistry Communications (rok: 2014, tom: 49, strony: 103-106), Wydawca: ScienceDirect
    Status:
    Published
    DOI:
    10.007 - link to the publication
  9. Apparent Anomalous Diffusion in the Cytoplasm of Human Cells: The Effect of Probes' Polydispersity
    Authors:
    Kalwarczyk Tomasz; Kwapiszewska Karina; Szczepanski Krzysztof; Sozanski Krzysztof; Szymański Jędrzej; Michalska Bernadeta; Patalas-Krawczyk Paulina; Duszynski Jerzy; Holyst Robert
    Academic press:
    JOURNAL OF PHYSICAL CHEMISTRY B (rok: 2017, tom: 121, strony: 9831-9837), Wydawca: AMER CHEMICAL SOC
    Status:
    Published
    DOI:
    10.1021/acs.jpcb.7b07158 - link to the publication
  10. Interaction of Mitochondria with the Endoplasmic Reticulum and Plasma Membrane in Calcium Homeostasis, Lipid Trafficking and Mitochondrial Structure
    Authors:
    Szymanski Jędrzej; Janikiewicz Justyna; Michalska Bernadeta; Patalas-Krawczyk Paulina; Perrone Mariasole; Ziolkowski Wieslaw; Duszynski Jerzy; Pinton Paolo; Dobrzyn Agnieszka; Wieckowski Mariusz R.
    Academic press:
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (rok: 2017, tom: 18, strony: 1576), Wydawca: MDPI AG
    Status:
    Published
    DOI:
    10.3390/ijms18071576 - link to the publication
  11. Manipulation of multiple-responsive fluorescent supramolecular materials based on the inclusion complexation of cyclodextrins with Tyloxapol
    Authors:
    Jinglin Shen, Jinyu Pang, Tomasz Kalwarczyk, Robert Hołyst, Xia Xin, Guiying Xu, Xiaoyu Luan and Yingjie Yang
    Academic press:
    Journal of Materials Chemistry C (rok: 2015, tom: 3, strony: 8104-8113), Wydawca: ROYAL SOC CHEMISTRY
    Status:
    Published
    DOI:
    10.1039/C5TC01826A - link to the publication
  12. Determination of oligomerization state of Drp1 protein in living cells at nanomolar concentrations
    Authors:
    Karina Kwapiszewska, Tomasz Kalwarczyk, Bernadeta Michalska, Krzysztof Szczepański, Jędrzej Szymański, Paulina Patalas-Krawczyk, Tomasz Andryszewski, Michalina Iwan, Jerzy Duszyński & Robert Hołyst
    Academic press:
    Scientific Reports (rok: 2019, tom: 9, strony: 5906), Wydawca: Nature Publishing Group
    Status:
    Published
    DOI:
    10.1038/s41598-019-42418-0 - link to the publication
  13. Mitochondria-associated membranes in aging and senescence: structure, function, and dynamics
    Authors:
    Janikiewicz J, Szymański J, Malinska D, Patalas-Krawczyk P, Michalska B, Duszyński J, Giorgi C, Bonora M, Dobrzyn A, Wieckowski MR.
    Academic press:
    Cell Death & Disease (rok: 2018, tom: 9, strony: 332), Wydawca: Nature Publishing Group
    Status:
    Published
    DOI:
    10.1038/s41419-017-0105-5 - link to the publication
  14. Photoactive Langmuir−Blodgett, Freely Suspended and Free Standing Films of Carboxylate Ligand-Coated ZnO Nanocrystals
    Authors:
    Jan Paczesny, Małgorzata Wolska-Pietkiewicz, Ilona Binkiewicz, Monika Wadowska, Zbigniew Wróbel, Kinga Matuła, Wojciech Nogala, Janusz Lewiński, and Robert Hołyst
    Academic press:
    ACS Applied Materials & Interfaces (rok: 2016, tom: 8, strony: 13532−13541), Wydawca: ACS Publications
    Status:
    Published
    DOI:
    10.1021/acsami.6b03579 - link to the publication
  1. Diffusion of Drp1 protein in the living cell
    Authors:
    Bernadeta Michalska, Jerzy Duszyński, Jędrzej Szymański
    Conference:
    2016 EBEC Abstracts (rok: 2016, ), Wydawca: Elsevier
    Data:
    konferencja Lipiec 2-7
    Status:
    Published
  2. Quantitative analysis of Drp1 oligomeric states in the living cell
    Authors:
    Bernadeta Michalska, Jerzy Duszyński, Jędrzej Szymański
    Conference:
    BIO 2016 Congress (rok: 2016, ), Wydawca: Polskie Towarzystwo Biochemiczne i Komitet Biochemii i Biofizyki PAN
    Data:
    konferencja 13-16.09
    Status:
    Published
  3. Insight into Drp1 mediated mitochondrial fission from FCS calibrated imaging
    Authors:
    Michalska, B.; Duszynski, J.; Szymanski, J.
    Conference:
    42nd Congress of the Federation-of-European-Biochemical-Societies (FEBS) on From Molecules to Cells and Back (rok: 2017, ), Wydawca: WILEY
    Data:
    konferencja SEP 10-14, 2017
    Status:
    Published
  4. Measurements of intracellular mobility of proteins associated with glycogen
    Authors:
    Paulina Patalas-Krawczyk, Jerzy Duszyński, Jędrzej Szymański
    Conference:
    BIO 2016 Congress (rok: 2016, ), Wydawca: Polskie Towarzystwo Biochemiczne i Komitet Biochemii i Biofizyki PAN
    Data:
    konferencja 13-16.09
    Status:
    Published

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