Projects funded by the NCN
New beta-lactoglobulin variants with increased affinity to selected highly toxic drugs
2012/05/B/ST5/00278
Keywords:
protein lactoglobulin lipocalin mutation toxicity crytal structure
Descriptors:
- ST5_16:
- ST5_11: Smart materials – self assembled materials, external stimuli-responsive materials
- ST5_20:
Panel:
ST5 - Materials: materials synthesis, structure-properties relations, advanced and functional materials with designed properties, (macro)molecular architecture, material engineering
Host institution :
Uniwersytet Jagielloński, Wydział Chemii
woj. małopolskie
Principal investigator (from the host institution):
Number of co-investigators in the project: 8
Call: OPUS 3 - announced on 2012-03-15
Amount awarded: 568 132 PLN
Project start date (Y-m-d): 2013-03-22
Project end date (Y-m-d): 2016-10-21
Project duration:: 43 months (the same as in the proposal)
Project status: Project settled
Equipment purchased [PL]
- Mały autoklaw laboratoryjny (14 000 PLN)
- Mikroogniskowa lampa rentgenowska (42 666 PLN)
- Zestaw komputerowy z drukarką laserową (9 000 PLN)
- Termocykler (15 017 PLN)
- Zestaw mini do elektroforezy DNA (3 000 PLN)
- Główka goniometryczna (2 000 PLN)
- Niskotemperaturowa zamrażarka laboratoryjna (-80°C) (35 000 PLN)
- Program do modelowania struktury kompleksów białka z ligandem (5 000 PLN)
- Specjalistyczny pH-metr do pomiaru pH lepkich cieczy (4 000 PLN)
- Spektrofotometr UV-VIS do pomiaru próbek z mikro-objętości (66 000 PLN)
- Laboratoryjna pompa próżniowa (3 000 PLN)
Information in the final report
- Publication in academic press/journals (5)
- Structure of Two Crystal Forms of Sheep b-Lactoglobulin with EF-Loop in Closed ConformationAuthors:Joanna I. Loch, Marta Molenda, Magdalena Kopeć, Sylwia Świątek, Krzysztof LewińskiAcademic press:Biopolymers (rok: 2014, tom: 101, strony: 886-894), Wydawca: Wiley-BlackwellStatus:PublishedDOI:10.1002/bip.22471 - link to the publication
- The differences in binding 12 carbon aliphatic ligands by bovine β lactoglobulin isoform A and B studied by isothermal titration calorimetry and X-ray crystallographyAuthors:Joanna I. Loch, Piotr Bonarek, Agnieszka Polit, Sylwia Świątek, Marta Dziedzicka-Wasylewska and Krzysztof LewińskiAcademic press:Journal of Molecular Recognition (rok: 2013, tom: 26, strony: 357-367), Wydawca: Wiley-BlackwellStatus:PublishedDOI:10.1002/jmr.2280 - link to the publication
- Conformational variability of goat β-lactoglobulin:Crystallographic and thermodynamic studiesAuthors:Joanna I. Loch, Piotr Bonarek, Agnieszka Polit, Sylwia Świątek, Mateusz Czub, Mira Ludwikowska and Krzysztof LewińskiAcademic press:International Journal of Biological Macromolecules (rok: 2015, tom: 72, strony: 1283-1291), Wydawca: ElsevierStatus:PublishedDOI:10.031 - link to the publication
- Engineered β-Lactoglobulin Produced in E. coli: Purification, Biophysical and Structural CharacterisationAuthors:Joanna I. Loch, Piotr Bonarek, Magdalena Tworzydło, Agnieszka Polit, Barbara Hawro, Aneta Łach, Eryk Ludwin, Krzysztof LewińskiAcademic press:Molecular Biotechnology (rok: 2016, tom: 58, strony: 605-618), Wydawca: SpringerStatus:PublishedDOI:10.1007/s12033-016-9960-z - link to the publication
- β-Lactoglobulin interactions with local anaesthetic drugs - Crystallographic and calorimetric studiesAuthors:Joanna I. Loch, Piotr Bonarek, Agnieszka Polit, Mateusz Jabłoński, Mateusz Czub, Xinxia Ye and Krzysztof LewińskiAcademic press:International Journal of Biological Macromolecules (rok: 2015, tom: 80, strony: 87-94), Wydawca: ElsevierStatus:PublishedDOI:10.1016/j.ijbiomac.2015.06.013 - link to the publication