Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

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Protaminization of somatic cells nuclei as a model for nuclear reprogramming in somatic cell nuclear transfer.

2019/35/B/NZ3/02856

Keywords:

Nuclear reprogramming (NR) somatic cell nuclear transfer (SCNT) protamine 1,

Descriptors:

  • NZ3_013:

Panel:

NZ3 - Cellular and developmental biology: cell biology, developmental biology, ageing biology, neurobiology

Host institution :

Instytut Genetyki i Hodowli Zwierząt Polskiej Akademii Nauk

woj. mazowieckie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr hab. Marta Czernik 

Number of co-investigators in the project: 4

Call: OPUS 18 - announced on 2019-09-16

Amount awarded: 1 738 800 PLN

Project start date (Y-m-d): 2020-07-28

Project end date (Y-m-d): 2025-07-27

Project duration:: 60 months (the same as in the proposal)

Project status: Project completed

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Information in the final report

  • Publication in academic press/journals (10)
  • Book publications / chapters in book publications (2)
  1. Oocyte activation is a cytoplasm-confined event so far: what about the nucleus?
    Authors:
    Luisa Gioia, Luca Palazzese, Marta Czernik, Domenico Iuso, Helena Fulka, Josef Fulka Jr, and Pasqualino Loi
    Academic press:
    Reproduction (rok: 2024, tom: 2024 Mar 1;, strony: 167(3): e230360), Wydawca: Bioscientifica; BIOSCIENTIFICA LTD.; SOCIETY FOR REPRODUCTION AND FERTILITY
    Status:
    Published
    DOI:
    10.1530/REP-23-0360 - link to the publication
  2. Advances in induced anhydrobiosis for cell and gamete storage.
    Authors:
    Loi P, Palazzese L, Moncada M, Sterzo ML, Iuso D, Czernik M, Wakayama T, Kikawada T.
    Academic press:
    Trends Biotechnol (rok: 2025, tom: 5:S0167-7799(25)00047-2, strony: 00047-2), Wydawca: Cell Press
    Status:
    Published
    DOI:
    10.1016/j.tibtech.2025.02.010 - link to the publication
  3. Reviving vacuum-dried encapsulated ram spermatozoa via ICSI after 2 years of storage
    Authors:
    Luca Palazzese, Federica Turri, Debora Agata Anzalone, Joseph Saragusty, Jacques Bonnet, Marthe Colotte, Sophie Tuffet, Flavia Pizzi, Alessia Luciani, Kazutsugu Matsukawa, Marta Czernik, Pasqualino Loi
    Academic press:
    Frontiers in Veterinary Science (rok: 2023, tom: 10, strony: 1270266), Wydawca: Frontiers
    Status:
    Published
    DOI:
    10.3389/fvets.2023.1270266 - link to the publication
  4. 25th ANNIVERSARY OF CLONING BY SOMATIC-CELL NUCLEAR TRANSFER: Scientific and technological approaches to improve SCNT efficiency in farm animals and pets
    Authors:
    Loi P., Palazzese L., Scapolo P.A., Fulka J., Fulka H., Czernik M
    Academic press:
    Reproduction (rok: 2021, tom: 162, strony: 33-43), Wydawca: Society for Reproduction and Fertility
    Status:
    Published
    DOI:
    10.1530/REP-20-0653 - link to the publication
  5. Nucleus reprogramming/remodeling through selective enucleation (SE) of immature oocytes and zygotes: a nucleolus point of view
    Authors:
    Helena Fulka, Pasqualino Loi, Luca Palazzese, Michal Benc, Josef Fulka Jr Jr
    Academic press:
    The Journal of Reproduction and Development. (rok: 2022, tom: 1;68(3):, strony: 165-172), Wydawca: Society for Reproduction and Development
    Status:
    Published
    DOI:
    10.1262/jrd.2022-004 - link to the publication
  6. Omne vivum ex ovo: the oocyte reprogramming and remodeling activities.
    Authors:
    Helena Fulka, Pasqualino Loi, Marta Czernik, Azim Surani, Josef Fulka
    Academic press:
    Reproduction (rok: 2023, tom: 165(3),, strony: pp. R75–R89), Wydawca: Bioscientifica; BIOSCIENTIFICA LTD.; SOCIETY FOR REPRODUCTION AND FERTILITY
    Status:
    Published
    DOI:
    10.1530/REP-22-0124 - link to the publication
  7. Mitochondrial function and intracellular distribution is severely affected in in vitro cultured mouse embryos
    Authors:
    Marta Czernik, Dawid Winiarczyk, Silvestre Sampino , Paweł Gręda , Salvatore Parillo, Jacek Andrzej Modliński, Pasqualino Loi
    Academic press:
    Scientific Reports (rok: 2022, tom: 27;12(1), strony: 16152), Wydawca: Nature Publishing Groupe
    Status:
    Published
    DOI:
    10.1038/s41598-022-20374-6 - link to the publication
  8. Exogenous LEA proteins expression enhances cold tolerance in mammalian cells by reducing oxidative stress
    Authors:
    Lo Sterzo M, Iuso D, Palazzese L, Moncada M, Boffa F, Scudieri A, Gioia L, Czernik M, Loi P.
    Academic press:
    Scientific Reports (rok: 2025, tom: 15, strony: 3351), Wydawca: Springer Nature
    Status:
    Published
    DOI:
    10.1038/s41598-025-86499-6 - link to the publication
  9. Controlled spermatozoa-oocyte interaction improves embryo quality in sheep
    Authors:
    Anzalone D.A., Palazzese L., Czernik M., Sabatucci A., Valbonetti L., Capra E., Loi P
    Academic press:
    Scientific Reports (rok: 2021, tom: 11, strony: 22692), Wydawca: Nature
    Status:
    Published
    DOI:
    10.1038/s41598-021-02000-z - link to the publication
  10. Reviving vacuum-dried encapsulated ram spermatozoa via ICSI after 2 years of storage
    Authors:
    Luca Palazzese, Federica Turri, Debora Agata Anzalone, Joseph Saragusty, Jacques Bonnet, Marthe Colotte, Sophie Tuffet, Flavia Pizzi, Alessia Luciani, Kazutsugu Matsukawa, Marta Czernik, Pasqualino Loi
    Academic press:
    Frontiers in Veterinary Science (rok: 2023, tom: 10, strony: 1270266), Wydawca: Frontiers
    Status:
    Published
    DOI:
    10.3389/fvets.2023.1270266 - link to the publication
  1. Somatic Cell Nuclear Transfer Using Freeze-Dried Protaminized Donor Nuclei
    Authors:
    Luca Palazzese, Marta Czernik, Kazutsugu Matsukawa, Pasqualino Loi
    Book:
    Somatic Cell Nuclear Transfer Technology (rok: 2023, tom: 2647, strony: pp 211–224), Wydawca: Humana Press
    Status:
    Published
    DOI:
    0.1007/978-1-0716-3064-8_11. - link to the publication
  2. Somatic Cell Nuclear Transfer Using Freeze-Dried Protaminized Donor Nuclei
    Authors:
    Luca Palazzese, Marta Czernik, Kazutsugu Matsukawa, Pasqualino Loi
    Book:
    Somatic Cell Nuclear Transfer Technology (rok: 2023, tom: 2647, strony: pp 211–224), Wydawca: Humana Press
    Status:
    Published
    DOI:
    0.1007/978-1-0716-3064-8_11. - link to the publication

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