Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

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mRNA 5' end methylation status: towards deeper understanding of biological role and identification of synthetic mimics

2019/33/B/ST4/01843

Keywords:

nucleotide synthesis 5' kap w mRNA terapeutyczne mRNA złoża chromatografii powinowactwa metylacja translation efficiency immunogenecity

Descriptors:

  • ST4_9: Biological chemistry
  • ST4_10: Polymer and biopolymer chemistry
  • NZ1_2: Biochemistry

Panel:

ST4 - Chemistry: physical chemistry/chemical physics, theoretical chemistry, analytical chemistry, inorganic chemistry, organic chemistry, method development

Host institution :

Uniwersytet Warszawski, Centrum Nowych Technologii Uniwersytetu Warszawskiego

woj.

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Jacek Jemielity 

Number of co-investigators in the project: 6

Call: OPUS 17 - announced on 2019-03-15

Amount awarded: 1 995 440 PLN

Project start date (Y-m-d): 2020-02-20

Project end date (Y-m-d): 2024-02-19

Project duration:: 48 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Information in the final report

  • Publication in academic press/journals (17)
  1. 2′-O-Methylation of the second transcribed nucleotide within the mRNA 5′ cap impacts the protein production level in a cell-specific manner and contributes to RNA immune evasion
    Authors:
    Karolina Drazkowska, Rafal Tomecki, Marcin Warminski, Natalia Baran, Dominik Cysewski, Anaïs Depaix, Renata Kasprzyk, Joanna Kowalska, Jacek Jemielity, Pawel J. Sikorski
    Academic press:
    Nucleic Acids Research (rok: 2022, tom: 50(16), strony: 9051–9071), Wydawca: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/nar/gkac722 - link to the publication
  2. Chemically Modified Poly(A) Analogs Targeting PABP: Structure Activity Relationship and Translation Inhibitory Properties
    Authors:
    Olga Perzanowska, Miroslaw Smietanski, Jacek Jemielity, Joanna Kowalska
    Academic press:
    Chemistry—A European Journal (rok: 2022, tom: 28 (42), strony: e202201115), Wydawca: Wiley-VCH GmbH
    Status:
    Published
    DOI:
    10.1002/chem.202201115 - link to the publication
  3. Novel N7-Arylmethyl Substituted Dinucleotide mRNA 50 cap Analogs: Synthesis and Evaluation as Modulators of Translation
    Authors:
    1. Wójcik R., Baranowski M., Markiewicz Ł., Kubacka D., Bednarczyk M., Baran N., Wojtczak A., Sikorski P.J., Żuberek J., Kowalska J., Jemielity J.
    Academic press:
    Pharmaceutics (rok: 2021, tom: 13(11), strony: 1941), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/pharmaceutics13111941 - link to the publication
  4. Chemically Modified Poly(A) Analogs Targeting PABP: Structure Activity Relationship and Translation Inhibitory Properties
    Authors:
    Olga Perzanowska, Miroslaw Smietanski, Jacek Jemielity, Joanna Kowalska
    Academic press:
    Chemistry—A European Journal (rok: 2022, tom: 28 (42), strony: e202201115), Wydawca: Wiley-VCH GmbH
    Status:
    Published
    DOI:
    10.1002/chem.202201115 - link to the publication
  5. Quick Access to Nucleobase-Modified Phosphoramidites for the Synthesis of Oligoribonucleotides Containing Post-Transcriptional Modifications and Epitranscriptomic Marks
    Authors:
    Kamil Ziemkiewicz, Marcin Warminski, Radoslaw Wojcik, Joanna Kowalska, Jacek Jemielity
    Academic press:
    The Journal of Organic Chemistry (rok: 2022, tom: 87 (15), strony: 10333-10348), Wydawca: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/acs.joc.2c01390 - link to the publication
  6. Preparation of RNAs with non-canonical 5′ ends using novel di- and trinucleotide reagents for co-transcriptional capping
    Authors:
    Anaïs Depaix, Ewa Grudzien-Nogalska, Bartlomiej Fedorczyk, Megerditch Kiledjian, Jacek Jemielity, Joanna Kowalska
    Academic press:
    FRONTIERS IN MOLECULAR BIOSCIENCES (rok: 2022, tom: 9, strony: Article Number 854170), Wydawca: FRONTIERS MEDIA SA
    Status:
    Published
    DOI:
    10.3389/fmolb.2022.854170 - link to the publication
  7. Novel N7-Arylmethyl Substituted Dinucleotide mRNA 50 cap Analogs: Synthesis and Evaluation as Modulators of Translation
    Authors:
    1. Wójcik R., Baranowski M., Markiewicz Ł., Kubacka D., Bednarczyk M., Baran N., Wojtczak A., Sikorski P.J., Żuberek J., Kowalska J., Jemielity J.
    Academic press:
    Pharmaceutics (rok: 2021, tom: 13(11), strony: 1941), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/pharmaceutics13111941 - link to the publication
  8. 2′-O-Methylation of the second transcribed nucleotide within the mRNA 5′ cap impacts the protein production level in a cell-specific manner and contributes to RNA immune evasion
    Authors:
    Karolina Drazkowska, Rafal Tomecki, Marcin Warminski, Natalia Baran, Dominik Cysewski, Anaïs Depaix, Renata Kasprzyk, Joanna Kowalska, Jacek Jemielity, Pawel J. Sikorski
    Academic press:
    Nucleic Acids Research (rok: 2022, tom: 50(16), strony: 9051–9071), Wydawca: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/nar/gkac722 - link to the publication
  9. Novel N7-Arylmethyl Substituted Dinucleotide mRNA 50 cap Analogs: Synthesis and Evaluation as Modulators of Translation
    Authors:
    1. Wójcik R., Baranowski M., Markiewicz Ł., Kubacka D., Bednarczyk M., Baran N., Wojtczak A., Sikorski P.J., Żuberek J., Kowalska J., Jemielity J.
    Academic press:
    Pharmaceutics (rok: 2021, tom: 13(11), strony: 1941), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/pharmaceutics13111941 - link to the publication
  10. Stereocontrolled access to thioisosteres of nucleoside di- and triphosphates
    Authors:
    Hai-Jun Zhang, Michał Ociepa, Molhm Nassir, Bin Zheng, Sarah A. Lewicki, Veronica Salmaso, Helay Baburi, Jessica Nagel, Salahuddin Mirza, Beatriz Bueschbell, Haneen Al-Hroub, Olga Perzanowska, Ziqin Lin, Michael A. Schmidt, Martin D. Eastgate, Kenneth A. Jacobson, Christa E. Müller, Joanna Kowalska, Jacek Jemielity & Phil S. Baran
    Academic press:
    Nature Chemistry (rok: 2023, tom: 16, strony: 249–258), Wydawca: Springer Nature
    Status:
    Published
    DOI:
    10.1038/s41557-023-01347-2 - link to the publication
  11. Quick Access to Nucleobase-Modified Phosphoramidites for the Synthesis of Oligoribonucleotides Containing Post-Transcriptional Modifications and Epitranscriptomic Marks
    Authors:
    Kamil Ziemkiewicz, Marcin Warminski, Radoslaw Wojcik, Joanna Kowalska, Jacek Jemielity
    Academic press:
    The Journal of Organic Chemistry (rok: 2022, tom: 87 (15), strony: 10333-10348), Wydawca: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/acs.joc.2c01390 - link to the publication
  12. RNA Ligation for Mono and Dually Labeled RNAs
    Authors:
    Depaix A. Młynarska-Cieślak A., Warmiński M., Sikorski P.J., Jemielity J., Kowalska J.
    Academic press:
    Chemistry - A European Journal (rok: 2021, tom: 27, strony: 12190-12197), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/chem.202101909 - link to the publication
  13. RNA Ligation for Mono and Dually Labeled RNAs
    Authors:
    Depaix A. Młynarska-Cieślak A., Warmiński M., Sikorski P.J., Jemielity J., Kowalska J.
    Academic press:
    Chemistry - A European Journal (rok: 2021, tom: 27, strony: 12190-12197), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/chem.202101909 - link to the publication
  14. Trinucleotide mRNA Cap Analogue N6-Benzylated at the Site of Posttranscriptional m6Am Mark Facilitates mRNA Purification and Confers Superior Translational Properties In Vitro and In Vivo
    Authors:
    Marcin Warminski, Edyta Trepkowska, Miroslaw Smietanski, Pawel J. Sikorski, Marek R. Baranowski, Marcelina Bednarczyk, Hanna Kedzierska, Bartosz Majewski, Adam Mamot, Diana Papiernik, Agnieszka Popielec, Remigiusz A. Serwa, Brittany A. Shimanski, Piotr Sklepkiewicz, Marta Sklucka, Olga Sokolowska, Tomasz Spiewla, Diana Toczydlowska-Socha, Zofia Warminska, Karol Wolosewicz, Joanna Zuberek, Jeffrey S. Mugridge, Dominika Nowis*, Jakub Golab*, Jacek Jemielity*, and Joanna Kowalska*
    Academic press:
    J. Am. Chem. Soc. (rok: 2024, tom: 146, strony: 8149–8163), Wydawca: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/jacs.3c12629 - link to the publication
  15. 1 of 1 Chemical Modifications of mRNA Ends for Therapeutic Applications
    Authors:
    Marcin Warminski, Adam Mamot, Anaïs Depaix, Joanna Kowalska and Jacek Jemielity
    Academic press:
    Accounts of Chemical Research (rok: 2023, tom: Volume 56 Issue 20, strony: 2814-2826), Wydawca: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/acs.accounts.3c00442 - link to the publication
  16. Preparation of RNAs with non-canonical 5′ ends using novel di- and trinucleotide reagents for co-transcriptional capping
    Authors:
    Anaïs Depaix, Ewa Grudzien-Nogalska, Bartlomiej Fedorczyk, Megerditch Kiledjian, Jacek Jemielity, Joanna Kowalska
    Academic press:
    FRONTIERS IN MOLECULAR BIOSCIENCES (rok: 2022, tom: 9, strony: Article Number 854170), Wydawca: FRONTIERS MEDIA SA
    Status:
    Published
    DOI:
    10.3389/fmolb.2022.854170 - link to the publication
  17. RNA Ligation for Mono and Dually Labeled RNAs
    Authors:
    Depaix A. Młynarska-Cieślak A., Warmiński M., Sikorski P.J., Jemielity J., Kowalska J.
    Academic press:
    Chemistry - A European Journal (rok: 2021, tom: 27, strony: 12190-12197), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/chem.202101909 - link to the publication

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