Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Local structural transitions in DNA molecule upon damage and repair

2018/31/B/ST4/02292

Keywords:

DNA tip-ehanced Raman surface-enhanced Raman infrared spectroscopy DNA damage and repair DNA conformational transition

Descriptors:

  • ST4_13: Solid-state chemistry, surface physicochemistry
  • ST4_3: Molecular architecture and structure
  • ST4_4: Theoretical and computational chemistry

Panel:

ST4 - Chemistry: physical chemistry/chemical physics, theoretical chemistry, analytical chemistry, inorganic chemistry, organic chemistry, method development

Host institution :

Uniwersytet Jagielloński, Wydział Fizyki, Astronomii i Informatyki Stosowanej

woj. małopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Marek Szymoński 

Number of co-investigators in the project: 8

Call: OPUS 16 - announced on 2018-09-14

Amount awarded: 2 075 854 PLN

Project start date (Y-m-d): 2019-09-30

Project end date (Y-m-d): 2023-03-29

Project duration:: 42 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. oprogramowanie PLS Toolbox, Mia Toolbox (Eigenvector) (4 szt.) (26 500 PLN)
  2. Mikroskop ze skanującą sondą wraz z akcesoriami, model "Omega Scope".
  3. System do wytrawiania chemicznego srebrnych próbników STM (22 500 PLN)
  4. program SPIP (Image metrology) (4 szt.) (55 000 PLN)
  5. Piezoelektryczny skaner do pozycjonowania wiązki lasera ramanowskiego, instalacja wymaga także zakupu nowego kontrolera AFM/STM (243 500 PLN)
  6. Skaner piezo XYZ pracujący w pętli zamkniętej wraz z akcesoriami.
  7. Kamera CCD z systemem chłodzenia (130 000 PLN)
  8. System to wytrawiania chemicznego złotych próbników STM (22 500 PLN)

Information in the final report

  • Publication in academic press/journals (10)
  1. Alterations in DNA conformation and histone phosphorylation resulting from double-strand breaks induced in HeLa cells – a spectroscopic and immunostaining approach
    Authors:
    Michał Czaja, Magdalena Giergiel, Katarzyna Skirlińska-Nosek, Kamila Sofińska, Dawid Lupa, Sara Seweryn, Olga Adamczyk, Zenon Rajfur, Giovanni Birarda, Federica Piccirilli, Lisa Vaccari, Marek Szymoński, Ewelina Lipiec
    Academic press:
    Cell Chemical Biology , Wydawca: Cell Press
    Status:
    Submitted
    DOI:
    brak - link to the publication
  2. Molecular Spectroscopic Markers of Abnormal Protein Aggregation
    Authors:
    Natalia Wilkosz, Michał Czaja, Sara Seweryn, Katarzyna Skirlinska-Nosek, Marek Szymonski, Ewelina Lipiec, Kamila Sofinska
    Academic press:
    Molecules (rok: 2020, tom: 25, strony: 2498, 1-26), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/molecules25112498 - link to the publication
  3. Molecular Spectroscopic Markers of DNA Damage
    Authors:
    Kamila Sofinska , Natalia Wilkosz, Marek Szymonski, Ewelina Lipiec
    Academic press:
    Molecules (rok: 2020, tom: 25, strony: 561, 1-24), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/molecules25030561 - link to the publication
  4. Plasmonic hot spots reveal local conformational transitions induced by DNA double-strand breaks
    Authors:
    Sara Seweryn, Katarzyna Skirlinska-Nosek, Natalia Wilkosz, Kamila Sofinska, David Perez-Guaita, Magdalena Ocwieja, Jakub Barbasz, Marek Szymonski, Ewelina Lipiec
    Academic press:
    Scientific Reports (rok: 2022, tom: 12, strony: 12158), Wydawca: Nature Publishing Group
    Status:
    Published
    DOI:
    10.1038/s41598-022-15313-4 - link to the publication
  5. Revealing DNA Structure at Liquid/Solid Interfaces by AFM-Based High-Resolution Imaging and Molecular Spectroscopy
    Authors:
    Ewelina Lipiec, Kamila Sofińska, Sara Seweryn, Natalia Wilkosz and Marek Szymoński
    Academic press:
    Molecules (rok: 2021, tom: 26(21), strony: 6476), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/molecules26216476 - link to the publication
  6. Morphological and molecular alterations in metaphase chromosomes induced by bleomycin
    Authors:
    Marta Urbańska, Michał Czaja, Kamila Sofińska, Krzysztof Szymoński, Dawid Lupa, Sara Seweryn, Katarzyna Skirlińska - Nosek, Marek Szymoński, Ewelina Lipiec
    Academic press:
    Spectrochemica Acta Part A: Molecular and Biomolecular Spectroscopy , Wydawca: Elsevier
    Status:
    Submitted
    DOI:
    brak - link to the publication
  7. Spectroscopic screening of pancreatic cancer
    Authors:
    Krzysztof Szymoński, Ewelina Lipiec, Kamila Sofińska, Katarzyna Skirlińska-Nosek, Katarzyna Milan-Ciesielska, Joanna Szporc, Michał Czaja, Sara Seweryn, Natalia Wilkosz, Giovanni Buarda, Federica Piccirilli, Lisa Vaccari, Marek Szymoński
    Academic press:
    Clinical Spectroscopy (rok: 2021, tom: 3, strony: 100016(4)), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.clispe.2021.100016 - link to the publication
  8. Detection of bleomycin-induced DNA strand breaks and repair processes in living cells by Raman microscopy
    Authors:
    Michał Czaja, Katarzyna Skirlińska-Nosek, Olga Adamczyk, Kamila Sofińska, Natalia Wilkosz, Zenon Rajfur, Marek Szymoński, Ewelina Lipiec
    Academic press:
    International Journal of Molecular Sciences (rok: 2022, tom: 23, strony: 3524(1-21)), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms23073524 - link to the publication
  9. Double-Strand Breaks Quantification by Statistical Length Analysis of DNA Fragments Imaged with AFM
    Authors:
    Kamila Sofińska, Michał Cieśla, Jakub Barbasz, Natalia Wilkosz, Ewelina Lipiec, Marek Szymoński, and Piotr Białas
    Academic press:
    Measurement (rok: 2022, tom: 198, strony: 111362), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.measurement.2022.111362 - link to the publication
  10. Optimization of tip-enhanced Raman spectroscopy for probing the chemical structure of DNA
    Authors:
    Sara Seweryn, Katarzyna Skirlinska-Nosek, Kamila Sofinska, Konrad Szajna, Jan Kobierski, Kamil Awsiuk, Marek Szymonski, Ewelina Lipiec
    Academic press:
    Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy (rok: 2022, tom: 281, strony: 121595), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.saa.2022.121595 - link to the publication

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