Projects funded by the NCN


Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Lsr2 protein as a global chromosomal organizer and transcription factor in Mycobacterium - an interplay of Lsr2 with SMC complex and HupB

2017/25/B/NZ1/00657

Keywords:

Lsr2 HupB SMC bacterial chromosome Mycobacterium transcription factor

Descriptors:

  • NZ1_10: Microbiology
  • NZ1_1: Molecular biology

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

Uniwersytet Wrocławski, Wydział Biotechnologii

woj. dolnośląskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Jolanta Zakrzewska-Czerwińska 

Number of co-investigators in the project: 6

Call: OPUS 13 - announced on 2017-03-15

Amount awarded: 1 906 000 PLN

Project start date (Y-m-d): 2018-01-10

Project end date (Y-m-d): 2021-07-09

Project duration:: 42 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. System Mikroprzepływowy CellASIC ONIX 2 wraz z podstawowym zestawem drenów (100 000 PLN)
  2. Zestaw do elektroforezy kapilarnej do analizy jakości kwasów nukleinowych (150 000 PLN)
  3. Homogenizator do wydajnej i powtarzalnej lizy komórek Mycobacterium (60 000 PLN)
  4. System dokumentacji żeli agarozowych.
  5. komputer.

Information in the final report

  • Publication in academic press/journals (7)
  1. Lsr2, a nucleoid-associated protein influencing mycobacterial cell cycle
    Authors:
    Kołodziej M, Trojanowski D, Bury K, Hołówka J, Matysik W, Kąkolewska H, Feddersen H, Giacomelli G, Konieczny I, Bramkamp M, Zakrzewska-Czerwińska J.
    Academic press:
    Sci Rep. (rok: 2021, tom: 11(1), strony: 2910), Wydawca: Nature Publishing Group
    Status:
    Published
    DOI:
    10.1038/s41598-021-82295-0 - link to the publication
  2. Lsr2 and its novel paralogue mediate the adjustment of Mycobacterium smegmatis to unfavorable environmental conditions
    Authors:
    Marta Kołodziej, Tomasz Łebkowski, Przemysław Płociński, Joanna Hołówka, Mariola Paściak, Bartosz Wojtaś, Katarzyna Bury, Igor Konieczny, Jarosław Dziadek, Jolanta Zakrzewska-Czerwińska
    Academic press:
    mSphere (rok: 2021, tom: 6 (3), strony: e00290-21), Wydawca: ASM
    Status:
    Published
    DOI:
    10.1128/mSphere.00290-21 - link to the publication
  3. Where and When Bacterial Chromosome Replication Starts: A Single Cell Perspective
    Authors:
    Damian Trojanowski, Joanna Hołowka Jolanta Zakrzewska-Czerwińska
    Academic press:
    Front. Microbiol. (rok: 2018, tom: 9, strony: 2819), Wydawca: Frontiers
    Status:
    Published
    DOI:
    10.3389/fmicb.2018.02819 - link to the publication
  4. Thiosemicarbazide Derivatives Decrease the ATPase Activity of Staphylococcus aureus Topoisomerase IV, Inhibit Mycobacterial Growth, and Affect Replication in Mycobacterium smegmatis
    Authors:
    Aleksandra Kowalczyk, Agata Paneth, Damian Trojanowski, Piotr Paneth, Jolanta Zakrzewska-Czerwińska, Paweł Stączek
    Academic press:
    Interenational Journal of Molecular Sciences (rok: 2021, tom: 22(8), strony: 3881), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms22083881 - link to the publication
  5. Nucleoid Associated Proteins: The Small Organizers That Help to Cope With Stress
    Authors:
    Joanna Hołówka, Jolanta Zakrzewska-Czerwińska
    Academic press:
    Front. Microbiol. (rok: 2020, tom: 11, strony: 45298), Wydawca: Frontiers
    Status:
    Published
    DOI:
    10.3389/fmicb.2020.00590 - link to the publication
  6. Watching DNA Replication Inhibitors in Action: Exploiting Time-Lapse Microfluidic Microscopy as a Tool for Target-Drug Interaction Studies in Mycobacterium
    Authors:
    Damian Trojanowski, Marta Kołodziej, Joanna Hołówka, Rolf Müller, Jolanta Zakrzewska-Czerwińska
    Academic press:
    Antimicrob Agents Chemother (rok: 2019, tom: 63(10), strony: 45307), Wydawca: ASM
    Status:
    Published
    DOI:
    10.1128/AAC.00739-19 - link to the publication
  7. Molekularne podstawy organizacji chromatyny bakteryjnej
    Authors:
    Marta Kołodziej Jolanta Zakrzewska-Czerwinska, Joanna Hołówka
    Academic press:
    Postępy Biochemii (rok: 2019, tom: 65, strony: 202-211), Wydawca: Polskie Towarzystwo Biochemiczne
    Status:
    Published
    DOI:
    10.18388/pb.2019_270 - link to the publication