Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Cotranscriptional folding of influenza virus RNA in MDCK cells in presence of antisense oligonucleotides. A new method for next-generation sequencing based chemical mapping of RNA structure.

2017/25/B/NZ1/02269

Keywords:

RNA influenza virus therapeutic oligonucleotides PVP induced RNA mapping next-generation sequencing cotranscriptional RNA folding secondary structure of RNA

Descriptors:

  • NZ1_2: Biochemistry
  • NZ1_4: Structural biology
  • NZ1_1: Molecular biology

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

woj. wielkopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Ryszard Kierzek 

Number of co-investigators in the project: 5

Call: OPUS 13 - announced on 2017-03-15

Amount awarded: 2 068 520 PLN

Project start date (Y-m-d): 2018-01-30

Project end date (Y-m-d): 2022-01-29

Project duration:: 48 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. Aparat do elektroforezy poliakrylamidowej (16 000 PLN)
  2. Oprogramowanie do komputera.
  3. Aparat Real-Time PCR.
  4. Inkubator z dwutlenkiem węgla (35 000 PLN)
  5. Spektrofotometr UV/VIS z termoprogramatorem i kuwetami kwarcowymi (142 000 PLN)
  6. Wysokiej sprawności komputer, monitor i drukarka laserowa (12 000 PLN)
  7. Zasilacz wysokonapięciowy (9 000 PLN)
  8. Aspirator próżniowy (12 000 PLN)
  9. Komora laminarna II klasa bezpieczeństwa (45 000 PLN)
  10. Pompa olejowa wysokiej próżni (10 000 PLN)

Information in the final report

  • Publication in academic press/journals (10)
  1. RNA Secondary Structure Motifs of the Influenza A Virus as Targets for siRNA-Mediated RNA Interference.
    Authors:
    J. Piasecka, E. Lenartowicz, M. Soszynska-Jozwiak, B. Szutkowska, R. Kierzek, E. Kierzek
    Academic press:
    Molecular Therapy-Nucleic Acids (rok: 2020, tom: 19, strony: 627-642), Wydawca: Cell Press
    Status:
    Published
    DOI:
    10.1016/j.omtn.2019.12.018 - link to the publication
  2. RNA Secondary Structure as a First Step for Rational Design of the Oligonucleotides towards Inhibition of Influenza A Virus Replication.
    Authors:
    M. Szabat, D. Lorent, T. Czapik, M. Tomaszewska, E. Kierzek, R. Kierzek
    Academic press:
    Pathogenes (rok: 2020, tom: 9, strony: 925), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/pathogens9110925 - link to the publication
  3. Secondary Structure of Influenza A Virus Genomic Segment 8 RNA Folded in a Cellular Environment
    Authors:
    B. Szutkowska, K. Wieczorek, R. Kierzek, P. Zmora, J. M. Peterson, W. N. Moss, D. H. Mathews, E. Kierzek
    Academic press:
    International Journal of Molecular Sciences (rok: 2022, tom: 23, strony: 2452), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms23052452 - link to the publication
  4. The regulation properties of RNA secondary structure in alternative splicing
    Authors:
    N. Bartys, R. Kierzek, J. Lisowiec-Wachnicka
    Academic press:
    Biochimica et biophysica acta. Gene regulatory mechanisms (rok: 2019, tom: 1862, strony: 194401), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bbagrm.2019.07.002 - link to the publication
  5. Computational and NMR studies of RNA duplexes with an internal pseudouridine-adenosine base pair
    Authors:
    I. Deb, L. Popenda, J. Sarzynska, M. Malgowska, A. Lahiri, Z. Gdaniec, R. Kierzek
    Academic press:
    Scientific Reports (rok: 2019, tom: 9, strony: 16278), Wydawca: Nature Publishing Group
    Status:
    Published
    DOI:
    10.1038/s41598-019-52637-0 - link to the publication
  6. Conserved Structural Motifs of Two Distant IAV Subtypes in Genomic Segment 5 RNA
    Authors:
    P. Michalak, J. Piasecka, B. Szutkowska, R. Kierzek, E. Biala, W.N. Moss, E. Kierzek
    Academic press:
    VIRUSES-BASEL (rok: 2021, tom: 13, strony: 2000-2016), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/v13030525 - link to the publication
  7. Thermodynamic and structural contributions of the 6-thioguanosine residue to helical properties of RNA
    Authors:
    M. Gladysz, W. Andralojc, T. Czapik, Z. Gdaniec, R. Kierzek
    Academic press:
    Scientific Reports (rok: 2019, tom: 9, strony: 4385), Wydawca: Nature Publishing Group
    Status:
    Published
    DOI:
    10.1038/s41598-019-40715-2 - link to the publication
  8. Universal and strain specific structure features of segment 8 genomic RNA of influenza A virus - application of 4-thiouridine photocrosslinking
    Authors:
    M. Soszynska-Jozwiak, M. Pszczola, J. Piasecka, J.M. Peterson, W.N. Moss, K. Taras-Goslinska, R. Kierzek, E. Kierzek
    Academic press:
    Journal of Biological Chemistry (rok: 2021, tom: 297, strony: 101245), Wydawca: ASBMB
    Status:
    Published
    DOI:
    10.1016/j.jbc.2021.101245 - link to the publication
  9. Structural variants and modifications of hammerhead ribozymes targeting influenza A virus conserved structural motifs
    Authors:
    T. Czapik, J. Piasecka, R. Kierzek, E. Kierzek
    Academic press:
    Molecular Therapy - Nucleic Acids (tom: brak, strony: brak), Wydawca: Elsevier Inc
    Status:
    Submitted
    DOI:
    brak - link to the publication
  10. Secondary structure of the segment 5 genomic RNA of influenza A virus and its application for designing antisense oligonucleotides
    Authors:
    P. Michalak, M. Soszynska-Jozwiak, E. Biala, W.N. Moss, J. Kesy, B. Szutkowska, E. Lenartowicz, R. Kierzek, E. Kierzek
    Academic press:
    Scientific Reports (rok: 2019, tom: 9, strony: 3801), Wydawca: Nuture Publishing Group
    Status:
    Published
    DOI:
    10.1038/s41598-019-40443-7 - link to the publication

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