Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

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Development of derivatization strategy and ultrasensitive analysis by mass spectrometry of podocin peptide fragments as biomarkers of preeclampsia

2016/23/B/ST4/01036

Keywords:

ionization enhancers mass spectrometry preeclampsia

Descriptors:

  • ST4_6: Instrumental methods in chemistry
  • NZ5_5: Diagnostics in human diseases
  • ST5_23:

Panel:

ST4 - Chemistry: physical chemistry/chemical physics, theoretical chemistry, analytical chemistry, inorganic chemistry, organic chemistry, method development

Host institution :

Uniwersytet Wrocławski, Wydział Chemii

woj. dolnośląskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Zbigniew Szewczuk 

Number of co-investigators in the project: 9

Call: OPUS 12 - announced on 2016-09-15

Amount awarded: 1 332 600 PLN

Project start date (Y-m-d): 2017-08-16

Project end date (Y-m-d): 2021-08-15

Project duration:: 48 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. Wirówka laboratoryjna (35 000 PLN)
  2. Wysokowydajny komputer (5 000 PLN)
  3. Komputer przenośny (laptop) (3 400 PLN)
  4. Wysokosprawny chromatograf cieczowy nano-LC umożliwiający połączenie ze spektrometrem mas (499 000 PLN)
  5. Holter ciśnieniowy (5 000 PLN)
  6. Zamrażarka LIEBHNERA.
  7. Cieplarka hodowlana z dopływem gazów.

Information in the final report

  • Publication in academic press/journals (14)
  • Articles in post-conference publications (1)
  1. Enrichment of cysteine-containing peptide by on-resin capturing and fixed charge tag derivatization for sensitive ESI-MS detection.
    Authors:
    Bąchor Remigiusz, Gorzeń Oliwia, Rola Anna, Mojsa Karolina, Panek-Laszczyńska Karolina, Konieczny Andrzej, Dąbrowska Krystyna, Witkiewicz Wojciech, Szewczuk Zbigniew
    Academic press:
    Molecules (rok: 2020, tom: 25, strony: 1372/1-1372/10), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/molecules25061372 - link to the publication
  2. Evaluation of podocin in urine in horses using qualitative and quantitative methods.
    Authors:
    Siwińska Natalia, Pasławska Urszula, Bąchor Remigiusz, Szczepankiewicz Barbara, Żak Agnieszka, Grocholska Paulina, Szewczuk Zbigniew
    Academic press:
    PLoS One (rok: 2020, tom: 15, strony: e0240586/1-e0240586/22), Wydawca: PLOSONE
    Status:
    Published
    DOI:
    10.1371/journal.pone.0240586 - link to the publication
  3. Preparation of isotopically labelled standards of creatinine via H/D exchange and their application in the quantitative analysis by LC-MS
    Authors:
    Remigiusz Bąchor, Andrzej Konieczny, Zbigniew Szewczuk
    Academic press:
    Molecules (rok: 2020, tom: 25, strony: 1514/1-1514/10), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/molecules25071514 - link to the publication
  4. Trends in the hydrogen−deuterium exchange at the carbon centers. Preparation of internal standards for quantitative analysis by LC-MS.
    Authors:
    Grocholska Paulina, Bąchor Remigiusz
    Academic press:
    Molecules (rok: 2021, tom: 26, strony: 2989/1-2989/25), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/molecules26102989 - link to the publication
  5. A non-covalent dimer formation of quaternary ammonium cation with unusual charge neutralization in electrospray-ionization mass spectrometry.
    Authors:
    Grocholska Paulina, Kowalska Marta, Wieczorek Robert, Bąchor Remigiusz
    Academic press:
    Molecules (rok: 2021, tom: 26, strony: 5868/1-5868/9), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/molecules26195868 - link to the publication
  6. Isobaric duplex based on a combination of 16O/18O enzymatic exchange and labeling with pyrylium salts
    Authors:
    Waliczek Mateusz, Bąchor Remigiusz, Kijewska Monika, Gąszczyk Dorota, Panek-Laszczyńska Karolina, Konieczny Andrzej, Dąbrowska Krystyna, Witkiewicz Wojciech, Marek-Bukowiec Karolina, Tracz Joanna, Łuczak Magdalena, Szewczuk Zbigniew, Stefanowicz Piotr
    Academic press:
    Analytica Chimica Acta (rok: 2019, tom: 1048, strony: 96-104), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.012 - link to the publication
  7. Detection of podocin in human urine sediment samples by charge derivatization and LC-MS-MRM method.
    Authors:
    Bąchor Remigiusz, Gąszczyk Dorota, Panek-Laszczyńska Karolina, Konieczny Andrzej, Witkiewicz Wojciech, Stefanowicz Piotr, Szewczuk Zbigniew
    Academic press:
    International Journal of Molecular Sciences (rok: 2020, tom: 21, strony: 3225/1-3225/13), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms21093225 - link to the publication
  8. Early detection of active glomerular lesions in dogs and cats using podocin.
    Authors:
    Szczepankiewicz Barbara, Pasławska Urszula, Nowak Marcin, Bąchor Remigiusz, Czyżewska-Buczyńska Agnieszka, Pasławski Robert, Szewczuk Zbigniew
    Academic press:
    Journal of Veterinary Research (rok: 2019, tom: 63, strony: 573-577), Wydawca: Sciendo
    Status:
    Published
    DOI:
    10.2478/jvetres-2019-0062 - link to the publication
  9. Identyfication of tryptic podocin peptide in the feline urine sediments using LC-MS/MRM method
    Authors:
    Bąchor Remigiusz, Szczepankiewicz Barbara, Pasławska Urszula, Mojsa Karolina, Stefanowicz Piotr, Szewczuk Zbigniew
    Academic press:
    International Journal of Mass Spectrometry (rok: 2019, tom: 444, strony: 116174/1-116174/6), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.ijms.2019.116174 - link to the publication
  10. Podocyturia as an early diagnostic marker of preeclampsia: a literature review
    Authors:
    Karolina Panek–Laszczyńska, Andrzej Konieczny, Ewa Milewska, Krystyna Dabrowska, Remigiusz Bąchor, Wojciech Witkiewicz, Zbigniew Szewczuk
    Academic press:
    Biomarkers (rok: 2018, tom: 23, strony: 207-212), Wydawca: Taylor&Francis
    Status:
    Published
    DOI:
    10.1080/1354750X.2017.1405283 - link to the publication
  11. Trends in the design of new isobaric labeling reagents for quantitative proteomics
    Authors:
    Bąchor Remigiusz, Waliczek Mateusz, Stefanowicz Piotr, Szewczuk Zbigniew
    Academic press:
    Molecules (rok: 2019, tom: 24, strony: 701/1-701/19), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/molecules24040701 - link to the publication
  12. Analysis of fragmentation pathways of peptide modified with quaternary ammonium and phosphonium group as ionization enhancers
    Authors:
    Monika Kijewska, Dorota Gąszczyk, Remigiusz Bąchor, Piotr Stefanowicz, Zbigniew Szewczuk
    Academic press:
    Molecules (rok: 2021, tom: 21, strony: 6964/1-6964/23), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/molecules26226964 - link to the publication
  13. Evaluation of tryptic podocin peptide in urine sediment using LC-MS-MRM method as a potential biomarker of glomerular injury in dogs with clinical signs of renal and cardiac disorders
    Authors:
    Szczepankiewicz Barbara, Bąchor Remigiusz, Pasławski Robert, Siwińska Natalia, Pasławska Urszula, Konieczny Andrzej, Szewczuk Zbigniew
    Academic press:
    Molecules (rok: 2019, tom: 24, strony: 3088/1-3088/16), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/molecules24173088 - link to the publication
  14. Peptide charge derivatization as a tool for early detection of preeclampsia by mass spectrometry - comparison with ELISA test
    Authors:
    Paulina Grocholska, Andrzej Konieczny, Zuzanna Kaźmierczak, Krystyna Dąbrowska, Karolina Pa-nek-Laszczyńska, Marlena Kłak, Wojciech Witkiewicz, Zbigniew Szewczuk, Remigiusz Bąchor
    Academic press:
    Molecules (rok: 2021, tom: 26, strony: 7102/1-7102/14), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/molecules26237102 - link to the publication
  1. Quaternary ammonium isobaric labeling for a relative and absolute quantification of peptides
    Authors:
    Mateusz Waliczek, Bartosz Setner, Monika Kijewska, Remigiusz Bąchor, Dorota Gąszczyk, Piotr Stefanowicz and Zbigniew Szewczuk
    Conference:
    35 th EUROPEAN PEPTIDE SYMPOSIUM (rok: 2018, ), Wydawca: Willey
    Data:
    konferencja 26-31.10
    Status:
    Published

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