Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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The mechanism of regioselective oxidative dehydrogenation of 3-ketosteroids catalyzed by D1-cholest-4-en-3-one dehydrogenase from Sterolibacterium denitrificans

2016/21/B/ST4/03798

Keywords:

flavin enzymes oxidative dehydrogenastion molecular modeling kinetic test point mutation

Descriptors:

  • ST4_15: Colloid chemistry
  • ST4_14: Catalysis
  • NZ9_9: Environmental biotechnology

Panel:

ST4 - Chemistry: physical chemistry/chemical physics, theoretical chemistry, analytical chemistry, inorganic chemistry, organic chemistry, method development

Host institution :

Instytut Katalizy i Fizykochemii Powierzchni im. Jerzego Habera Polskiej Akademii Nauk

woj. małopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Maciej Szaleniec 

Number of co-investigators in the project: 5

Call: OPUS 11 - announced on 2016-03-15

Amount awarded: 970 840 PLN

Project start date (Y-m-d): 2017-02-23

Project end date (Y-m-d): 2021-05-22

Project duration:: 51 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. Spektrofotometr UV-vis z przystawką temperaturową (100 000 PLN)
  2. Komputer sterujący PC.
  3. 4-kanałowy kontroler do fermentora (25 000 PLN)
  4. Chromatograf FPLC do preparatywnego oczyszczania białek (280 000 PLN)
  5. Komputer PC do modelowania molekularnego (4 500 PLN)
  6. licencja Gaussian View W6.

Information in the final report

  • Publication in academic press/journals (6)
  • Book publications / chapters in book publications (1)
  1. Enzymatic Δ1-dehydrogenation of 3-ketosteroids – reconciliation of kinetic isotope effects with the reaction mechanism
    Authors:
    Michał Glanowski, Patrycja Wójcik, Magdalena Procner, Maria Oszajca, Katarzyna Świderek, Vicent Moliner, Andrzej J. Bojarski, Maciej Szaleniec
    Academic press:
    ACS Catalysis (rok: 2021, tom: 11, strony: 8211−8225), Wydawca: ACS
    Status:
    Published
    DOI:
    10.1021/acscatal.1c01479 - link to the publication
  2. Structure and reaction mechanism of 3-ketosteroid Δ1-dehydrogenase from Sterolibacterium denitrificans
    Authors:
    Patrycja Wójcik, Michał Glanowski, Beata Mrugała, Magdalena Procner, Olga Zastawny, Monika Flejszar, Katarzyna Kurpiewska, Ewa Niedziałkowska, Wladek Minor, Maria Oszajca, Andrzej J. Bojarski, Agnieszka M. Wojtkiewicz, Maciej Szaleniec
    Academic press:
    ACS Catalysis (rok: 2022, ), Wydawca: ACS
    Status:
    Submitted
    DOI:
    brak - link to the publication
  3. Investigation of quaternary structure of aggregating 3-ketosteroid dehydrogenase from Sterolibacterium denitrificans: In the pursuit of consensus of various biophysical techniques
    Authors:
    Kamila Sofińska, Agnieszka M. Wojtkiewicz, Patrycja Wójcik, Olga Zastawny, Maciej Guzik, Agnieszka Winiarska, Piotr Waligórski, Michał Cieśla, Jakub Barbasz, Maciej Szaleniec
    Academic press:
    BBA - General Subjects (rok: 2019, tom: 1863, strony: 1027-1039), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bbagen.2019.03.009 - link to the publication
  4. Universal Capability of 3-Ketosteroid Δ1- Dehydrogenases to Catalyze Δ1-Dehydrogenation of C17- Substituted Steroids
    Authors:
    P. Wójcik, M. Glanowski, A. M. Wojtkiewicz, A. Rohman, M. Szaleniec
    Academic press:
    Microbial Cell Factories (rok: 2021, tom: 20, strony: 119), Wydawca: BMC
    Status:
    Published
    DOI:
    10.1186/s12934-021-01611-5 - link to the publication
  5. Application of Immobilized Cholest-4-En-3-One D1- Dehydrogenase from Sterolibacterium Denitrificans for Dehydrogenation of Steroids
    Authors:
    M. Tataruch, P. Wójcik, A. M. Wojtkiewicz, K. Zaczyk, K. Szymańska, M. Szaleniec
    Academic press:
    Catalysts (rok: 2020, tom: 10(12), strony: 1460), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/catal10121460 - link to the publication
  6. The efficient Δ1-dehydrogenation of a wide spectrum of 3-ketosteroids in a broad pH range by 3-ketosteroid dehydrogenase from Sterolibacterium denitrificans
    Authors:
    Agnieszka M. Wojtkiewicz, Patrycja Wójcik, Magdalena Procner, Monika Flejszar, Maria Oszajca, Mateusz Hochołowski, Mateusz Tataruch, Beata Mrugała, Tomasz Janeczko, Maciej Szaleniec
    Academic press:
    The Journal of Steroid Biochemistry and Molecular Biology (rok: 2020, tom: 202, strony: 105731), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.jsbmb.2020.105731 - link to the publication
  1. Model setup and procedures for prediction of enzyme reaction kinetics with QM-only and QM:MM approaches
    Authors:
    M. Glanowski, S. Kachhap, T. Borowski, M. Szaleniec
    Book:
    'Computational Methods for Estimating the Kinetics Parameters of Biological Systems' in 'Methods in Molecular Biology' (rok: 2022, tom: 2385, strony: 175-236), Wydawca: Springer US, Humana, New York, NY.
    Status:
    Published

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