Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Bifunctional antisense oligonucleotides - optimization of alternative splicing regulation in cancer cell lines

2016/21/D/NZ5/01906

Keywords:

bifunctional antisense oligonucleotides alternativ splicing canser modified oligonucleotides in vitro splicing assay canser cell lines

Descriptors:

  • NZ5_7: Human disease treatment

Panel:

NZ5 - Human and animal noninfectious diseases: etiology, mechanisms, diagnosis and treatment of diseases, poisonings and injuries (without neurological diseases)

Host institution :

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

woj. wielkopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr Jolanta Lisowiec-Wąchnicka 

Number of co-investigators in the project: 2

Call: SONATA 11 - announced on 2016-03-15

Amount awarded: 748 700 PLN

Project start date (Y-m-d): 2017-03-02

Project end date (Y-m-d): 2021-03-01

Project duration:: 48 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. blok grzejno-chłodzący z wytrząsaniem (12 000 PLN)
  2. termocykler PCR (20 000 PLN)
  3. wirówka laboratoryjna z chłodzeniem z dwoma rotorami (30 000 PLN)
  4. zestaw komputerowy (komputer, monitor, drukarka) (5 500 PLN)

Information in the final report

  • Publication in academic press/journals (7)
  1. Thermodynamic, anticoagulant and antiproliferative properties of thrombin binding aptamer analogues containing novel UNA derivative
    Authors:
    Kotkowiak W., Lisowiec-Wachnicka J., Grynda J., Kierzek R., Wengel J., Pasternak
    Academic press:
    Molecular Therapy: Nucleic Acid (rok: 2018, tom: 10, strony: 304-316), Wydawca: Molecular Therapy: Nucleic Acid
    Status:
    Published
    DOI:
    10.1016/j.omtn.2017.12. - link to the publication
  2. Gamper antisense oligonucleotides containing 2',3'-dideoxy-2'-fluoro-3'-C-hydroxymethyl-β-D-lyxofuranosyl nucleotides display site-specific RNase H cleavage and induce gene silencing
    Authors:
    Mathias B. Danielsen, Chenguang Lou, Jolanta Lisowiec-Wachnicka, Anna Pasternak, Per T. Jørgensen, Jesper Wengel
    Academic press:
    Chemistry – A European Journal (rok: 2019, tom: brak, strony: brak), Wydawca: WILEY-V C H VERLAG GMBH
    Status:
    Published
    DOI:
    10.1002/chem.201904540 - link to the publication
  3. Contribution of 3′T and 3′TT overhangs to the thermodynamic stability of model siRNA duplexes
    Authors:
    Jolanta Lisowiec-Wachnicka, Brent M. Znosko, Anna Pasternak
    Academic press:
    Biophysical Chemistry (rok: 2019, tom: 246, strony: 35-39), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bpc.2018.12.006 - link to the publication
  4. The regulation properties of RNA secondary structure in alternative splicing
    Authors:
    Natalia Bartyś, Ryszard Kierzek, Jolanta Lisowiec-Wąchnicka
    Academic press:
    BBA-Gene Regulatory Mechanism (rok: 2019, tom: 1862, strony: 194401), Wydawca: ELSEVIER SCIENCE BV
    Status:
    Published
    DOI:
    10.1016/j.bbagrm.2019.07.002 - link to the publication
  5. Influence of mismatched and bulged nucleotides on SNP preferential RNase H cleavage of RNA-antisense gapmer heteroduplexes
    Authors:
    Magner D., Biala E., Lisowiec-Wachnicka J., Kierzek R.
    Academic press:
    Scientific Reports (rok: 2017, tom: 7, strony: 12532), Wydawca: Scientific Reports
    Status:
    Published
    DOI:
    10.1038/s41598-017-12844-z - link to the publication
  6. A disease-causing intronic point mutation C19G alters tau exon 10 splicing via RNA secondary structure rearrangement
    Authors:
    Jiazi Tan, Lixia Yang, Alan Ann Lerk Ong, Jiahao Shi, Zhensheng Zhong, Mun Leng Lye, Shiyi Liu, Jolanta Lisowiec-Wachnicka, Ryszard Kierzek, Xavier Roca, and Gang Chen
    Academic press:
    Biochemistry (rok: 2019, tom: 58, strony: 1565-1578), Wydawca: AMER CHEMICAL SOC
    Status:
    Published
    DOI:
    10.1021/acs.biochem.9b00001 - link to the publication
  7. A systematic study on the influence of thermodynamic asymmetry of 5′-ends of siRNA duplexes in relation to their silencing potency
    Authors:
    Jolanta Lisowiec-Wąchnicka, Natalia Bartyś, Anna Pasternak
    Academic press:
    Nature (rok: 2019, tom: 9, strony: 2477), Wydawca: Nature
    Status:
    Published
    DOI:
    10.1038/s41598-018-36620-9 - link to the publication

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