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Interactions of the potassium channel ROMK2 with mitochondrial proteins

2015/19/B/NZ1/02794

Keywords:

mitochondria potassium channels ATP

Descriptors:

  • NZ1_3: Biophysics
  • NZ1_2: Biochemistry
  • NZ1_1: Molecular biology

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

Instytut Biologii Doświadczalnej im. M. Nenckiego Polskiej Akademii Nauk

woj. mazowieckie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr Piotr Koprowski 

Number of co-investigators in the project: 3

Call: OPUS 10 - announced on 2015-09-15

Amount awarded: 875 800 PLN

Project start date (Y-m-d): 2016-07-12

Project end date (Y-m-d): 2020-07-11

Project duration:: 48 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. System do pionowej elektroforezy z chłodząco-grzejącą łaźnią cyrkulacyjną (30 000 PLN)
  2. -.
  3. Termomikser (25 000 PLN)
  4. Inkubator CO2 do hodowli komórkowych.

Information in the final report

  • Publication in academic press/journals (7)
  • Articles in post-conference publications (9)
  • Book publications / chapters in book publications (1)
  1. Electrochemical studies of the mitochondrial ROMK2 potassium channel activity reconstituted into the free-standing and tethered bilayer lipid membranes
    Authors:
    Aleksandra Stefanowska, Piotr Koprowski, Piotr Bednarczyk, Adam Szewczyk, Pawel Krysinski
    Academic press:
    Bioelectrochemistry (rok: 2023, tom: 151, strony: 108372), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bioelechem.2023.108372 - link to the publication
  2. Mitochondrial potassium channels – an overview
    Authors:
    Szewczyk A, Bednarczyk P, Jędraszko J, Kampa RP, Koprowski P, Krajewska M, Kucman S, Kulawiak B, Laskowski M, Rotko D, Sęk A, Walewska A, Żochowska M, Wrzosek A.
    Academic press:
    Postepy Biochemii (rok: 2018, tom: 64(3), strony: 196-212), Wydawca: Polskie Towarzystwo Biochemiczne
    Status:
    Published
    DOI:
    10.18388/pb.2018_132 - link to the publication
  3. Pharmacological Characterization of a Recombinant Mitochondrial ROMK2 Potassium Channel Expressed in Bacteria and Reconstituted in Planar Lipid Bilayers
    Authors:
    Milena Krajewska, Adam Szewczyk, Bogusz Kulawiak, Piotr Koprowski
    Academic press:
    Membranes (rok: 2023, tom: 13, strony: 360), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/membranes13030360 - link to the publication
  4. Gas Signaling Molecules and Mitochondrial Potassium Channels
    Authors:
    Agnieszka Walewska, Adam Szewczyk, Piotr Koprowski
    Academic press:
    International Journal of Molecular Sciences (rok: 2018, tom: 19(10), strony: 3227-3247), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms19103227 - link to the publication
  5. Solubilization, purification, and functional reconstitution of human ROMK potassium channel in copolymer styrene-maleic acid (SMA) nanodiscs
    Authors:
    Krajewska M., Koprowski P.
    Academic press:
    Biochimica et Biophysica Acta - Biomembranes (rok: 2021, tom: 1863(4), strony: -), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bbamem.2021.183555 - link to the publication
  6. Interaction of ROMK2 channel with lipid kinases DGKE and AGK: potential channel activation by localized anionic lipid synthesis
    Authors:
    Milena Krajewska, Mariusz Możajew, Sławomir Filipek, Piotr Koprowski
    Academic press:
    Journal of Molecular Biology (rok: 2023, tom: brak, strony: brak), Wydawca: Elsevier
    Status:
    Submitted
    DOI:
    brak - link to the publication
  7. Mechanosensitivity of mitochondrial largeconductance calcium-activated potassium channels
    Authors:
    Agnieszka Walewska, Bogusz Kulawiak, Adam Szewczyk, Piotr Koprowski
    Academic press:
    Biochimica et Biophysica Acta (BBA) - Bioenergetics (rok: 2018, tom: 9, strony: 797-805), Wydawca: Elsevier B.V.
    Status:
    Published
    DOI:
    10.1016/j.bbabio.2018.05.006 - link to the publication
  1. Characterization of ROMK2 protein activity
    Authors:
    Milena Krajewska, Piotr Bednarczyk, Piotr Koprowski, Adam Szewczyk
    Conference:
    Tools for Structural Biology of Membrane – EMBO Wookshop (rok: 2019, ), Wydawca: EMBO/EMBL
    Data:
    konferencja 43745
    Status:
    Published
  2. Production of ROMK1/2 protein in E. coli for functional studies
    Authors:
    Milena Krajewska, Piotr Koprowski, Adam Szewczyk
    Conference:
    20th European Bioenergetics Conference, EBEC 2018 (rok: 2018, ), Wydawca: Elsevier B. V.
    Data:
    konferencja 25-30 sierpnia
    Status:
    Published
  3. Regulation of activity of mitochondrial potassium channels
    Authors:
    Piotr Koprowski, Milena Krajewska, Agnieszka Walewska, Adam Szewczyk
    Conference:
    Mitoest 2018 (rok: 2018, ), Wydawca: National Institute of Chemical Physics and Biophysics
    Data:
    konferencja 43214
    Status:
    Published
  4. Bio-ID as a method to search for partners of mitochondrial channels
    Authors:
    Krajewska M., Żochowska M., Koprowski P., Szewczyk A.
    Conference:
    6th Mitochondrion 2017 'Bioenergetics Update", 8.12.2017 (rok: 2017, ), Wydawca: Uniwersytet Adama Mickiewicza w Poznaniu
    Data:
    konferencja 08.12.2017
    Status:
    Published
  5. Synthesis of ROMK1/2 protein in Eschericha coli for functional studies
    Authors:
    Krajewska M, Koprowski P, Szewczyk A
    Conference:
    7th MITOCHONDRION 2018 "Mitochondria in the spotlight" (rok: 2018, ), Wydawca: Uniwersytet Wrocławski
    Data:
    konferencja 18-19 październik
    Status:
    Published
  6. Synthesis of Romk1/2 Protein in E.Coli
    Authors:
    Milena Krajewska, Piotr Koprowski, Adam Szewczyk
    Conference:
    63rd Annual Meeting of the Biophysical Society (rok: 2019, ), Wydawca: Biophysical Society
    Data:
    konferencja 43526
    Status:
    Published
  7. An attempt to isolate mitochondrial membrane protein complexes in membrane nanodiscs
    Authors:
    M. Krajewska, A. Kiełbasa, P. Koprowski, B. Kulawiak, A. Szewczyk
    Conference:
    42nd Congress of the Federation-of-European-Biochemical-Societies (FEBS) on From Molecules to Cells and Back (rok: 2017, ), Wydawca: FEBS Press
    Data:
    konferencja 10-14 września
    Status:
    Published
  8. Mitochondrial regulation of mitochondrial potassium channels
    Authors:
    Adam Szewczyk, Piotr Koprowski, Bogusz Kulawiak, Piotr Bednarczyk
    Conference:
    20th European Bioenergetics Conference, EBEC 2018 (rok: 2018, ), Wydawca: Elsevier B. V.
    Data:
    konferencja 25-30 sierpnia
    Status:
    Published
  9. Recording channel activity of the ROMK1/2 protein produced in E. coli
    Authors:
    Piotr Koprowski, Milena Krajewska, Adam Szewczyk
    Conference:
    20th European Bioenergetics Conference, EBEC 2018 (rok: 2018, ), Wydawca: Elsievier B. V.
    Data:
    konferencja 25-30 sierpnia
    Status:
    Published
  1. Patch-Clamp Recording of the Activity of Ion Channels in the Inner Mitochondrial Membrane
    Authors:
    Bednarczyk P., Kampa RP., Kucman S., Sęk A.,Walewska A., Koprowski P.
    Book:
    Weissig V., Edeas M. (eds) Mitochondrial Medicine. Methods in Molecular Biology (rok: 2021, tom: 2276, strony: -), Wydawca: V. Weissig, M. Edeas, Editors. Humana Press, Springer New York Heidelberg Dordrecht London
    Status:
    Published

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