Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Molecular basis of regulation of electron flow between membranous quinone and water-soluble cytochrome. Does mitochondrial complex III switch between "slow" and "fast" state?

2015/18/A/NZ1/00046

Keywords:

molecular bioenergetics electron transfer cytochrome c cytochrome bc1 chinone EPR mitochondrial electron transport chain

Descriptors:

  • NZ1_3: Biophysics
  • NZ1_2: Biochemistry
  • NZ1_1: Molecular biology

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

Uniwersytet Jagielloński, Wydział Biochemii, Biofizyki i Biotechnologii

woj. małopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Artur Osyczka 

Number of co-investigators in the project: 6

Call: MAESTRO 7 - announced on 2015-06-15

Amount awarded: 2 499 555 PLN

Project start date (Y-m-d): 2016-09-01

Project end date (Y-m-d): 2022-08-31

Project duration:: 72 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. Monochromator ze światłowodami (15 000 PLN)
  2. Spektrofotometr do mikroobjętości DS-11.
  3. Rezonator EPR Bruker ER4104OR (96 000 PLN)
  4. Mechaniczny homogenizator tkankowy (3 000 PLN)

Information in the final report

  • Publication in academic press/journals (11)
  • Book publications / chapters in book publications (2)
  1. Catalytic Reactions and Energy Conservation in the Cytochromebc1andb6fComplexes of Energy-Transducing Membranes
    Authors:
    Marcin Sarewicz, Sebastian Pintscher, Rafał Pietras, Arkadiusz Borek,Łukasz Bujnowicz, Guy Hanke,William A. Cramer, Giovanni Finazzi, Artur Osyczka
    Academic press:
    Chemical Reviews (rok: 2021, tom: 121, strony: 2020–2108), Wydawca: ACS
    Status:
    Published
    DOI:
    10.1021/acs.chemrev.0c00712 - link to the publication
  2. The high-spin heme bL mutant exposes dominant reaction leading to formation of the semiquinone spin coupled to [2Fe-2S]+ cluster at the Qo site of Rhodobacter capsulatus cytochrome bc1
    Authors:
    Marcin Sarewicz, Sebastian Pintscher, Łukasz Bujnowicz, Małgorzata Wolska, Artur Osyczka
    Academic press:
    Frontiers in Chemistry (rok: 2021, tom: 9, strony: 658877), Wydawca: Frontiers Media S.A.
    Status:
    Published
    DOI:
    10.3389/fchem.2021.658877 - link to the publication
  3. A spontaneous mitonuclear epistasis converging on Rieske Fe-S protein exacerbates complex III deficiency in mice
    Authors:
    J. Purhonen, V. Grigorjev, R. Ekiert, N. Aho, J. Rajendran, R. Pietras, K. Truve, M. Wkistrom, V. Sharma, A. Osyczka, V. Fellman, J. Kallijarvi
    Academic press:
    Nature Communications (rok: 2020, tom: 11, strony: 322), Wydawca: Springer Nature
    Status:
    Published
    DOI:
    10.1038/s41467-019-14201-2 - link to the publication
  4. Charge polarization imposed by the binding site facilitates enzymatic redox reactions of quinone
    Authors:
    Sebastian Pintscher, Anna Wójcik-Augustyn, Marcin Sarewicz, Artur Osyczka
    Academic press:
    Biochimica et Biophysica Acta -Bioenergetics (rok: 2020, tom: 1861, strony: 148216), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bbabio.2020.148216 - link to the publication
  5. Functional flexibility of electron flow between quinol oxidation Qo site of cytochrome bc1 and cytochrome c revealed by combinatory effects of mutations in cytochrome b, iron-sulfur protein and cytochrome c1
    Authors:
    Arkadiusz Borek, Robert Ekiert, Artur Osyczka
    Academic press:
    Biochimica et Biophysica Acta - Bioenergetics (rok: 2018, tom: 1859, strony: 754-761), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bbabio.2018.04.010 - link to the publication
  6. Hydrogen bonding rearrangement by a mitochondrial disease mutation in cytochrome bc1 perturbs heme bH redox potential and spin state
    Authors:
    Patryk Kuleta, Jonathan Lasham, Marcin Sarewicz, Iwona Ekiert, Vivek Sharma, Robert Ekiert, Artur Osyczka
    Academic press:
    Proceedings of National Academy of Sciences USA (rok: 2021, tom: 118, strony: e2026169118), Wydawca: National Academy of Sciences
    Status:
    Published
    DOI:
    10.1073/pnas.2026169118 - link to the publication
  7. Supression of superoxide production by a spin-spin coupling bewteen semiquinone and the Rieske cluster in cytochrome bc1
    Authors:
    Łukasz Bujnowicz, Arkadiusz Borek, Patryk Kuleta, Marcin Sarewicz, Artur Osyczka
    Academic press:
    Febs Letters (rok: 2019, tom: 593, strony: 45363), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/1873-3468.13296 - link to the publication
  8. Low-cost stopped-flow and freeze-quench device for double mixing
    Authors:
    Łukasz Bujnowicz,Rafał Pietras, Marcin Sarewicz, Artur Osyczka
    Academic press:
    Hardware X , Wydawca: Elsevier
    Status:
    Submitted
    DOI:
    brak - link to the publication
  9. Generation of semiquinone-[2Fe-2S]+ spin-coupled center at the Qo site of cytochrome bc1 in redox-poised, illuminated photosynthetic membranes from Rhodobacter capsulatus
    Authors:
    Marcin Sarewicz, Łukasz Sarewicz, Artur Osyczka
    Academic press:
    Biochimica et Biophysica Acta - Bioenergetics (rok: 2018, tom: 1859, strony: 145-153), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bbabio.2017.11.006 - link to the publication
  10. Identification of hydrogen bonding network for proton transfer at the quinol oxidation site of Rhodobacter capsulatus cytochrome bc1
    Authors:
    Arkadiusz Borek, Anna Wójcik-Augustyn, Patryk Kuleta, Robert Ekiert, Artur Osyczka
    Academic press:
    Biochmica et Biophysica Acta , Wydawca: Elsevier
    Status:
    Submitted
    DOI:
    brak - link to the publication
  11. Electron sweep across four b-hemes of cytochrome bc1 revealed by unusual paramagnetic properties of the Qi semiquinone intermediate
    Authors:
    Sebastian Pintscher, Rafał Pietras, Marcin Sarewicz, Artur Osyczka
    Academic press:
    Biochimica et Biophysica Acta - Bioenergetics (rok: 2018, tom: 1859, strony: 459-469), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bbabio.2018.03.010 - link to the publication
  1. Advances in understanding mechanism and physiology of cytochromes bc
    Authors:
    Arkadiusz Borek, Robert Ekiert, Artur Osyczka
    Book:
    Mechanisms of primary energy transduction in biology (rok: 2018, tom: 5, strony: 192-214), Wydawca: The Royal Society of Chemistry
    Status:
    Published
  2. Cytochrome bc1 complex (Respiratory Chain Complex III)
    Authors:
    Marcin Sarewicz, Artur Osyczka
    Book:
    Encyclopedia of Biological Chemistry, 3rd Edition (rok: 2021, tom: 2, strony: 502-511), Wydawca: Elsevier
    Status:
    Published

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