Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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The use of genetic tools in experimental therapy of polyglutamine diseases.

2015/18/E/NZ2/00678

Keywords:

RNAi shRNA CRISPR/Cas9 HD SCA3

Descriptors:

  • NZ2_1: Molecular genetics
  • NZ5_7: Human disease treatment
  • NZ1_1: Molecular biology

Panel:

NZ2 - Genetics, genomics: molecular genetics, genomics, proteomics, bioinformatics, systems biology, genetic epidemiology

Host institution :

Instytut Chemii Bioorganicznej PAN

woj. wielkopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr hab. Marta Olejniczak 

Number of co-investigators in the project: 4

Call: SONATA BIS 5 - announced on 2015-06-15

Amount awarded: 1 917 600 PLN

Project start date (Y-m-d): 2016-04-18

Project end date (Y-m-d): 2021-10-17

Project duration:: 66 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. aparat do elektroforezy westem blot dedykowany do rozdzialu bialek w komercyjnych żelach gradientowych.
  2. licznik komórek (15 000 PLN)
  3. termocykler (30 000 PLN)
  4. zestaw komputerowy wraz z oprogramowaniem (2 szt.) (9 000 PLN)
  5. Inkubator bakteryjny z wyposażeniem.
  6. aparat do elektroforezy i transferu białek z zasilaczem (30 000 PLN)
  7. zestaw adaptujący wieszak MTP do rotora wirówki.

Information in the final report

  • Publication in academic press/journals (9)
  • Articles in post-conference publications (2)
  • Book publications / chapters in book publications (1)
  1. Generation of human iPS cell line IBCHi001-A from dentatorubral-pallidoluysian atrophy patient's fibroblasts
    Authors:
    Kozlowska E, Ciolak A, Olejniczak M, Fiszer A.
    Academic press:
    Stem Cell Research (rok: 2019, tom: 39, strony: 101512), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.scr.2019.101512 - link to the publication
  2. Precise excision of the CAG tract from the Huntingtin Gene by Cas9 Nickases
    Authors:
    Dabrowska M, Juzwa W, Krzyzosiak WJ, Olejniczak M
    Academic press:
    Front Neurosci (rok: 2018, tom: 0,552083333333333, strony: 45299), Wydawca: Frontiers Media SA
    Status:
    Published
    DOI:
    10.3389/fnins.2018.00075 - link to the publication
  3. Generation of new isogenic models of Huntington's disease using CRISPR-Cas9 technology
    Authors:
    Dabrowska M, Ciolak A, Kozlowska E, Fiszer A. and Olejniczak M
    Academic press:
    International Journal of Molecular Sciences (rok: 2020, tom: 21(5), strony: 1854), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms21051854 - link to the publication
  4. Stress-induced changes in miRNA biogenesis and functioning
    Authors:
    Olejniczak M, Kotowska-Zimmer A, Krzyzosiak WJ
    Academic press:
    Cell Mol Life Sci., (rok: 2018, tom: 75, strony: 177-191), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s00018-017-2591-0 - link to the publication
  5. Universal RNAi Triggers for the Specific Inhibition of Mutant Huntingtin, Atrophin-1, Ataxin-3, and Ataxin-7 Expression
    Authors:
    Kotowska-Zimmer A, Ostrovska Y, Olejniczak M
    Academic press:
    Mol Ther Nucleic Acids (rok: 2020, tom: 19, strony: 562-571), Wydawca: CellPress
    Status:
    Published
    DOI:
    10.1016/j.omtn.2019.12.012 - link to the publication
  6. qEva-CRISPR: a method for quantitative evaluation of CRISPR/Cas-mediated genome editing in target and off-target sites
    Authors:
    Dabrowska M, Czubak K, Juzwa W, Krzyzosiak WJ, Olejniczak M, Kozlowski P
    Academic press:
    Nucleic Acids Res (rok: 2018, tom: 46(17), strony: e101), Wydawca: Oxford Academic
    Status:
    Published
    DOI:
    10.1093/nar/gky505 - link to the publication
  7. A CAG repeat-targeting artificial miRNA lowers the mutant huntingtin level in the YAC128 model of Huntington's disease
    Authors:
    Kotowska-Zimmer A., Przybyl L., Pewinska M., Suszynska-Zajczyk J., Wronka D., Figiel M. and Olejniczak M
    Academic press:
    Molecular Therapy Nucleic Acids (rok: 2022, ), Wydawca: Cell Press
    Status:
    Accepted for publication
    DOI:
    brak - link to the publication
  8. Artificial miRNAs as therapeutic tools: challenges and opportunities
    Authors:
    Kotowska-Zimmer A, Pewinska M, Olejniczak M
    Academic press:
    WIREs RNA (rok: 2021, tom: nie dotyczy, strony: nie dotyczy), Wydawca: Wiley Periodicals LLC
    Status:
    Published
    DOI:
    10.1002/wrna.1640 - link to the publication
  9. Paving the way towards precise and safe CRISPR genome editing
    Authors:
    Pawel Sledzinski, Magdalena Dabrowska, Mateusz Nowaczyk, Marta Olejniczak
    Academic press:
    Biotechnology Advances (rok: 2021, tom: 49, strony: 45311), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.biotechadv.2021.107737 - link to the publication
  1. A CAG repeat-targeting artificial miRNA lowers the mutant huntingtin level in the YAC128 model of Huntington's disease
    Authors:
    M. Olejniczak, A. Kotowska-Zimmer, L. Przybyl, M. Pewińska, J. Suszynska-Zajczyk, D. Wronka, M. Figiel
    Conference:
    European Huntington's Disease Network (EHDN) Meeting (rok: 2021, ), Wydawca: BMJ Journals
    Data:
    konferencja 9-11.09
    Status:
    Published
  2. Universal RNAi triggers for specific inhibition of mutant huntingtin, ataxin-3, ataxin-7 and atrophin-1 expression
    Authors:
    A. Kotowska-Zimmer, J. Ostrowska, K. Grochowina, A. Ciesiołka, P. Joachimiak, A. Fiszer, M. Olejniczak
    Conference:
    the 44th FEBS Congress (rok: 2019, ), Wydawca: FEBS Open Bio
    Data:
    konferencja 6-11.07.2019
    Status:
    Published
  1. Gene Therapy for Huntington's Disease Using Targeted Endonucleases
    Authors:
    Magdalena Dabrowska and Marta Olejniczak
    Book:
    Methods in Molecular Biology, Trinucleotide repeats (rok: 2020, tom: 2056, strony: 269-284), Wydawca: Humana Press
    Status:
    Published

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