Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Approach to structures of difficult protein targets using new mass spectrometry-based methods

2014/14/A/NZ1/00306

Keywords:

protein structure intermediate filament Alzheimer's disease histone pre-mRNA procentriole mass spectrometry intrinsically disordered proteins

Descriptors:

  • NZ1_3: Biophysics
  • NZ1_4: Structural biology

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

Instytut Biochemii i Biofizyki Polskiej Akademii Nauk

woj. mazowieckie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Michał Dadlez 

Number of co-investigators in the project: 6

Call: MAESTRO 6 - announced on 2014-06-16

Amount awarded: 3 189 880 PLN

Project start date (Y-m-d): 2015-04-29

Project end date (Y-m-d): 2020-04-28

Project duration:: 60 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Pompa turbomolekularna (2 szt.) (100 000 PLN)
  2. Liofilizator (40 000 PLN)
  3. Zamrażarka -80 oC (50 000 PLN)

Information in the final report

  • Publication in academic press/journals (18)
  1. His6, His13, and His14 residues in Aβ 1–40 peptide signifcantly and specifcally afect oligomeric equilibria
    Authors:
    Kaja Przygońska, Magdalena Pacewicz, Wiktoria Sadowska, Jarosław Poznański, Wojciech Bal, Michał Dadlez
    Academic press:
    Scientific Reports (rok: 2019, tom: 9: 9449 (13 p.), strony: 45304), Wydawca: Nature Research
    Status:
    Published
    DOI:
    10.1038/s41598-019-45988-1 - link to the publication
  2. Interaction interface in the C-terminal parts of centriole proteins Sas6 and Ana2
    Authors:
    Fatalska A, Dzhindzhev NS, Dadlez M, Glover DM.
    Academic press:
    Open Biol. (rok: 2020, tom: 10, strony: 200), Wydawca: The Royal Society
    Status:
    Published
    DOI:
    10.1098/rsob.200221 - link to the publication
  3. Two SnRK2-Interacting Calcium Sensor Isoforms Negatively Regulate SnRK2 Activity by Different Mechanisms
    Authors:
    Tarnowski K, Klimecka M, Ciesielski A, Goch G, Kulik A, Fedak H, Poznański J, Lichocka M, Pierechod M, Engh RA, Dadlez M, Dobrowolska G, Bucholc M.
    Academic press:
    Plant Physiol. (rok: 2019, tom: 182, strony: 1142), Wydawca: Oxford Academics
    Status:
    Published
    DOI:
    10.1104/pp.19.00900 - link to the publication
  4. U7 snRNP is recruited to histone pre-mRNA in a FLASH-dependent manner by two separate regions of the stem-loop binding protein.
    Authors:
    SKRAJNA A., YANG X-C., BUCHOLC K., ZHANG J., HALL T.M.T., DADLEZ M., MARZLUFF W.F., DOMIŃSKI Z.
    Academic press:
    RNA (rok: 2017, tom: 23 (6), strony: 938-951), Wydawca: Cold Spring Harbor Lab Press
    Status:
    Published
    DOI:
    10.1261/rna.060806.117 - link to the publication
  5. Side-chain moieties from the N-terminal region of A beta are involved in an oligomer-stabilizing network of interactions.
    Authors:
    PRZYGOŃSKA K., POZNAŃSKI J., MISTARZ U.H., RAND K.D., DADLEZ M.
    Academic press:
    PLOS ONE (rok: 2018, tom: brak, strony: 45316), Wydawca: Public Library of Science
    Status:
    Published
    DOI:
    10.1371/journal.pone.0201761 - link to the publication
  6. Vimentin S-glutathionylation at Cys328 inhibits filament elongation and induces severing of mature filaments in vitro
    Authors:
    Magdalena Kaus‐Drobek Norbert Mücke Roman H. Szczepanowski Tatjana Wedig Mariusz Czarnocki‐Cieciura Magdalena Polakowska Harald Herrmann Aleksandra Wysłouch‐Cieszyńska Michał Dadlez
    Academic press:
    The FEBS Journal (rok: 2020, tom: On line, strony: 45310), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1111/febs.15321 - link to the publication
  7. A Strong Neutrophil Elastase Proteolytic Fingerprint Marks the Carcinoma Tumor Proteome
    Authors:
    KISTOWSKI M., DĘBSKI J., KARCZMARSKI J., PAZIEWSKA A., OLĘDZKI J., MIKUŁA M., OSTROWSKI J., DADLEZ M.
    Academic press:
    Molecular & Cellular Proteomics (rok: 2017, tom: 16(2), strony: 213-227), Wydawca: American Society for Biochemistry and Molecular Biology
    Status:
    Published
    DOI:
    10.1074/mcp.M116.058818 - link to the publication
  8. Composition and processing activity of a semi-recombinant holo U7 snRNP.
    Authors:
    BUCHOLC K., AIK W.S., YANG X-C., WANG K., ZHOU Z.H., DADLEZ M., MARZLUFF W.F., TONG L., DOMIŃSKI Z.
    Academic press:
    Nucleic Acids Research (rok: 2020, tom: Vol. 48 no. 3, strony: 1508–1530), Wydawca: Oxford Academic
    Status:
    Published
    DOI:
    10.1093/nar/gkz1148 - link to the publication
  9. Fine-tuning of the stability of β-strands by Y181 in perfringolysin O directs the prepore to pore transition
    Authors:
    Kulma M, Kacprzyk-Stokowiec A, Traczyk G, Kwiatkowska K, Dadlez M.
    Academic press:
    Biochim Biophys Acta Biomembr. (rok: 2019, tom: 1861, strony: 110), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bbamem.2018.08.008 - link to the publication
  10. Protein composition of catalytically active U7-dependent processing complexes assembled on histone pre-mRNA containing biotin and a photo-cleavable linker
    Authors:
    SKRAJNA A., YANG X-C., DADLEZ M., MARZLUFF W.F., DOMIŃSKI Z.
    Academic press:
    Nucleic Acids Research (rok: 2018, tom: Vol. 46, No. 9, strony: 4752–4770), Wydawca: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/nar/gky133 - link to the publication
  11. R468A mutation in perfringolysin O destabilizes toxin structure and induces membrane fusion.
    Authors:
    KULMA M., KACPRZYK-STOKOWIEC A., KWIATKOWSKA K., TRACZYK G., SOBOTA A., DADLEZ M.
    Academic press:
    Biochim Biophys Acta Biomembr. (rok: 2017, tom: Vol. 1859,Iss 6, strony: 1075-1088), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bbamem.2017.03.001 - link to the publication
  12. Rattlesnake Phospholipase A2 Increases CFTR-Chloride Channel Current and Corrects ∆F508CFTR Dysfunction: Impact in Cystic Fibrosis
    Authors:
    Grazyna Faure, Naziha Bakouh, Stéphane Lourdel, Norbert Odolczyk, Aiswaraya Premchandar, Nathalie Servel, Aurélie Hatton, Maciej K. Ostrowski, Haijin Xu, Frederick Saul, Christelle Moquereau, Iwona Pranke, Gabrielle Planelles, Jacques Teulon, Harald Herrmann, Piotr Zielenkiewicz, Michal Dadlez, Gergely L. Lukacs, Isabelle Sermet-Gaudelus, Mario Ollero, Pierre-Jean Corringer, Aleksander Edelman
    Academic press:
    Journal of Molecular Biology (rok: 2016, tom: 428, Issue 14, strony: 2898–2915), Wydawca: Elsevier Wordmark
    Status:
    Published
    DOI:
    10.1016/j.jmb.2016.05.016 - link to the publication
  13. Structural Dynamics of the Vimentin Coiled-Coil Contact Regions involved in Filament Assembly as revealed by Hydrogen-Deuterium Exchange
    Authors:
    Aiswarya Premchandar, Norbert Mücke, Jarosław Poznański, Tatjana Wedig, Magdalena Kaus-Drobek, Harald Herrmann, Michał Dadlez
    Academic press:
    The Journal of Biological Chemistry (rok: 2016, tom: 291(48), strony: 24931–24950), Wydawca: The American Society for Biochemistry and Molecular Biolog
    Status:
    Published
    DOI:
    10.1074/jbc.M116.748145 - link to the publication
  14. Two-step phosphorylation of Ana2 by Plk4 is required for the sequential loading of Ana2 and Sas6 to initiate procentriole formation
    Authors:
    DZHINDZHEV N., TZOLOVSKY G., LIPINSZKI Z., ABDELAZIZ M., DĘBSKI J., DADLEZ M., GLOVER D.M.
    Academic press:
    Open Biology (rok: 2017, tom: 7 (12), strony: 45302), Wydawca: The Royal Society Publishing
    Status:
    Published
    DOI:
    10.1098/rsob.170247 - link to the publication
  15. Enhanced oligomerization of full-length RAGE by synergy of the interaction of its domains.
    Authors:
    MOYSA A., DIETMAR H., SZCZEPANOWSKI R.H., SOBOTT F., DADLEZ M.
    Academic press:
    Scientific Reports (rok: 2019, tom: 9:20332 (15 p.), strony: 45306), Wydawca: Nature Sesearch
    Status:
    Published
    DOI:
    10.1038/s41598-019-56993-9 - link to the publication
  16. Structural analysis of the SANT/Myb domain of FLASH and YARP proteins and their complex with the C-terminal fragment of NPAT by NMR spectroscopy and computer simulations
    Authors:
    Katarzyna Bucholc, Aleksandra Skrajna, Kinga Adamska, Xiao-Cui Yang, Krzysztof Krajewski, Jarosław Poznański, Michal Dadlez, Zbigniew Dominski, Igor Zhukov
    Academic press:
    International Journal of Molecular Sciences (rok: 2020, tom: 21, strony: 5268), Wydawca: Multidisciplinary Digital Publishing Institute
    Status:
    Published
    DOI:
    10.3390/ijms21155268 - link to the publication
  17. The dimeric Golgi protein Gorab binds to Sas6 as a monomer to mediate centriole duplication
    Authors:
    Fatalska A, Stepinac E, Richter M, Kovacs L, Pietras Z, Puchinger M, Dong G, Dadlez M, Glover DM
    Academic press:
    eLife (rok: 2021, tom: 10, strony: e57241), Wydawca: eLife Sciences publications
    Status:
    Published
    DOI:
    10.7554/eLife.57241 - link to the publication
  18. A model of full-length RAGE in complex with S100B
    Authors:
    Moysa A, Steczkiewicz K, Niedzialek D, Hammerschmid D, Zhukova L, Sobott F, Dadlez M.
    Academic press:
    Structure , Wydawca: Cell Press
    Status:
    Accepted for publication
    DOI:
    10.1016/j.str.2021.04.002. - link to the publication

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