Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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The role of antimicrobial protein-chemerin in skin pathophysiology.

2014/12/W/NZ6/00454

Keywords:

antimicrobial peptides skin epithelial tissue

Descriptors:

  • NZ6_4: Bacteriology
  • NZ4_4: Pathophysiology

Panel:

NZ6 - Human and animal immunology and infection: immunity, immune disorders, immunotherapy, infectious and invasive diseases, microbiology, transplantology, allergology

Host institution :

Uniwersytet Jagielloński, Wydział Biochemii, Biofizyki i Biotechnologii

woj. małopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Joanna Cichy 

Number of co-investigators in the project: 18

Call: SYMFONIA 2

Amount awarded: 6 999 560 PLN

Project start date (Y-m-d): 2014-11-21

Project end date (Y-m-d): 2021-02-20

Project duration:: 75 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Mikroskop biologiczny z kamerą cyfrową (16 728 PLN)
  2. Pompa strzykawkowe i robot do nanoszenia próbek. (60 000 PLN)
  3. System do analizy obrazów. (170 000 PLN)
  4. komputer stacjonarny z monitorem LCD.
  5. Platforma PCR do analiz w czasie rzeczywistym. (180 000 PLN)
  6. Stacja robocza i rozbudowa cytometru o nowe lasery.
  7. Łaźnia wodna (5 227 PLN)
  8. System do detekcji produktów PCR w czasie rzeczywistym. (120 000 PLN)
  9. Wielodetekcyjny czytnik mikropłytkowy (100 000 PLN)
  10. Zamrażarka (849 PLN)
  11. Aparat do kawitacji.
  12. Komputer stacjonarny (3 676 PLN)
  13. Komputer stacjonarny.
  14. Rozbudowa stacji roboczej (hardware i software).
  15. dwustanowiskowa komora laminarna.
  16. Przyrząd do mierzenia wilgotości skóry. (75 000 PLN)
  17. Stacja robocza i podajnik płytek do cytometru przepływowego LSRII. (140 000 PLN)
  18. komputer stacjonarny - 2 szt..

Information in the final report

  • Publication in academic press/journals (33)
  1. A passive microfluidic system based on step emulsification allows the generation of libraries of nanoliter-sized droplets from microliter droplets of varying and known concentrations of a sample
    Authors:
    W. Postek, T. Kamiński, P. Garstecki
    Academic press:
    Lab on a Chip (rok: 2017, tom: 17, strony: 1323-1331), Wydawca: ROYAL SOC CHEMISTRY
    Status:
    Published
    DOI:
    10.1039/C7LC00014F - link to the publication
  2. A precise and accurate microfluidic droplet dilutor
    Authors:
    Witold Postek, Tomasz S Kaminski and Piotr Garstecki
    Academic press:
    Analyst (rok: 2017, tom: 142, strony: 2901-2911), Wydawca: ROYAL SOC CHEMISTRY
    Status:
    Published
    DOI:
    10.1039/C7AN00679A - link to the publication
  3. Antimicrobial Properties of a Peptide Derived from the Male Fertility Factor kl2 Protein of Drosophila melanogaster
    Authors:
    Bilska, B.; Godlewska, U.; Damulewicz, M.; Murzyn, K.; Kwitniewski, M.; Cichy, J.; Pyza, E.
    Academic press:
    Current Issues in Molecular Biology (rok: 2022, tom: 44, strony: 1169-1181), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/cimb44030076 - link to the publication
  4. Oscillating droplet trains in microfluidic networks and their suppression in blood flow
    Authors:
    Bartosz Grzybowski, Olgierd Cybulski, Piotr Garstecki
    Academic press:
    Nature Physics (rok: 2019, tom: 15, strony: 706-713), Wydawca: Springer Nature
    Status:
    Published
    DOI:
    10.1038/s41567-019-0486-8 - link to the publication
  5. Recent developments of microfluidics as a tool for biotechnology and microbiology
    Authors:
    Ott Scheler, Witold Postek, Piotr Garstecki
    Academic press:
    Current Opinion in Biotechnology (rok: 2019, tom: 55, strony: 60-67), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.copbio.2018.08.004 - link to the publication
  6. Split or slip - passive generation of monodisperse double emulsions with cores of varying viscosity in microfluidic tandem step emulsification system
    Authors:
    Adam S. Opalski, Karol Makuch, Ladislav Derzsi and Piotr Garstecki
    Academic press:
    RSC Advances (rok: 2020, tom: 10, strony: 23058 - 23065), Wydawca: The Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/D0RA03007D - link to the publication
  7. The expression and regulation of chemerin in the epidermis.
    Authors:
    Banas M, Zegar A, Kwitniewski M, Zabieglo K, Marczynska J, Kapinska-Mrowiecka M, LaJevic M, Zabel BA, Cichy J.
    Academic press:
    PLos One (rok: 2015, tom: 10(2):e011783, strony: 45310), Wydawca: Plos.org
    Status:
    Published
    DOI:
    10.1371/journal.pone.0117830 - link to the publication
  8. The role of chemerin and its antibacterial derivatives in maintaining epithelial barrier function
    Authors:
    Godlewska U, Zegar A, Cichy J.
    Academic press:
    Postepy Biochemii (rok: 2020, tom: 66 (2), strony: 151-159), Wydawca: Polskie Towarzystwo Biochemiczne
    Status:
    Published
    DOI:
    10.18388/pb.2020_323 - link to the publication
  9. Bacteria Modify Their Sensitivity to Chemerin-Derived Peptides by Hindering Peptide Association With the Cell Surface and Peptide Oxidation
    Authors:
    Godlewska U, Bilska B, Majewski P, Pyza E, Zabel BA, Cichy J.
    Academic press:
    Frontiers in Microbiology (rok: 2020, tom: 11, strony: 1819 (1-11)), Wydawca: Frontiers
    Status:
    Published
    DOI:
    10.3389/fmicb.2020.01819 - link to the publication
  10. Controlled droplet microfluidic systems for multistep chemical and biological assays
    Authors:
    Tomasz S Kaminski and Piotr Garstecki
    Academic press:
    Chemical Society Reviews (rok: 2017, tom: 46, strony: 6210 - 6226), Wydawca: ROYALSOC CHEMISTRY
    Status:
    Published
    DOI:
    10.1039/C5CS00717H - link to the publication
  11. Expression of antimicrobial peptide genes oscillates along day/night rhythm protecting mice skin from bacteria
    Authors:
    Bilska B, Zegar A, Slominski AT, Kleszczyński K, Cichy J, Pyza E.
    Academic press:
    Experimental Dermatology (rok: 2020, ), Wydawca: John Wiley & Sons Ltd
    Status:
    Published
    DOI:
    10.1111/exd.14229 - link to the publication
  12. Grooved step emulsification systems optimize throughput of passive generation of monodisperse emulsions
    Authors:
    Adam S. Opalski, Karol Makuch, Yu-Kai Lai, Ladislav Derzsi and Piotr Garstecki
    Academic press:
    Lab on a Chip (rok: 2019, tom: 19, strony: 1183-1192), Wydawca: Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/C8LC01096J - link to the publication
  13. Passive and parallel microfluidic formation of droplet interface bilayers (DIBs) for measurement of leakage of small molecules through artificial phospholipid membranes
    Authors:
    Magdalena A. Czekalska, Tomasz S. Kaminski, Karol Makuch and Piotr Garstecki
    Academic press:
    Sensors and Actuators: B Chemical (rok: 2019, tom: 286, strony: 258-265), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.snb.2019.01.143 - link to the publication
  14. The methylation status of the chemerin promoter region located from - 252 to + 258 bp regulates constitutive but not acute-phase cytokine-inducible chemerin expression levels
    Authors:
    Kwiecien K, Brzoza P, Bak M, Majewski P, Skulimowska I, Bednarczyk K, Cichy J, Kwitniewski M.
    Academic press:
    Scientific Reports (rok: 2020, tom: 10, strony: 13702 (1-12)), Wydawca: Nature
    Status:
    Published
    DOI:
    10.1038/s41598-020-70625-7 - link to the publication
  15. A method for simultaneous polishing and hydrophobization of polycarbonate for microfluidic applications
    Authors:
    Dominika Ogończyk, Paweł Jankowski, Piotr Garstecki
    Academic press:
    Polymers (rok: 2020, tom: 12(11), strony: 2490), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/polym12112490 - link to the publication
  16. Direct droplet digital PCR (dddPCR) for species specific, accurate and precise quantification of bacteria in mixed samples
    Authors:
    Natalia Pacocha, Ott Scheler, Mikołaj Marcin Nowak, Ladislav Derzsi, Joanna Cichy, Piotr Garstecki
    Academic press:
    Analytical Methods (rok: 2019, tom: 11, strony: 5730-5735), Wydawca: ROYAL SOC CHEMISTRY
    Status:
    Published
    DOI:
    10.1039/C9AY01874C - link to the publication
  17. Dodecylresorufin (C12R) outperforms resorufin in micro-droplet bacterial assays
    Authors:
    O. Scheler, T.S. Kaminski, A. Ruszczak and P. Garstecki
    Academic press:
    Applied Materials and Interfaces (rok: 2016, tom: 8, strony: 11318-11325), Wydawca: AMER CHEMICAL SOC
    Status:
    Published
    DOI:
    10.1021/acsami.6b02360 - link to the publication
  18. Droplet Microfluidics as a Tool for the Generation of Granular Matters and Functional Emulsions
    Authors:
    Adam S. Opalski, Tomasz S. Kaminski, Piotr Garstecki
    Academic press:
    KONA Powder and Particle Journal (rok: 2018, tom: 45368, strony: 45313), Wydawca: Hosokawa Powder Technology Foundation
    Status:
    Published
    DOI:
    10.14356/kona.2019004 - link to the publication
  19. Droplet microfluidics for microbiology: techniques, applications and challenges
    Authors:
    T.S. Kaminski, O. Scheler and P. Garstecki
    Academic press:
    Lab on a Chip (rok: 2016, tom: 16, strony: 2168-2187), Wydawca: ROYAL SOC CHEMISTRY
    Status:
    Published
    DOI:
    10.1039/C6LC00367B - link to the publication
  20. Metagenomic Studies in Inflammatory Skin Diseases
    Authors:
    Godlewska U, Brzoza P, Kwiecień K, Kwitniewski M, Cichy J.
    Academic press:
    Current Microbiology (rok: 2020, tom: 77, strony: 3201–3212), Wydawca: Springer nature
    Status:
    Published
    DOI:
    10.1007/s00284-020-02163-4 - link to the publication
  21. Redox Active Antimicrobial Peptides in Controlling Growth of Microorganisms at Body Barriers
    Authors:
    Brzoza, P.; Godlewska, U.; Borek, A.; Morytko, A.; Zegar, A.; Kwiecinska, P.; Zabel, B.A.; Osyczka, A.; Kwitniewski, M.; Cichy, J.
    Academic press:
    Antioxidants (rok: 2021, tom: 10(3), strony: 446 (1-18)), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ - link to the publication
  22. The antimicrobial activity of chemerin-derived peptide p4 requires oxidative conditions.
    Authors:
    Urszula Godlewska, Bernadetta Bilska, Aneta Zegar, Piotr Brzoza, Arkadiusz Borek, Krzysztof Murzyn, Oliwia Bochenska, Agnieszka Morytko, Patryk Kuleta, Andrzej Kozik, Elzbieta Pyza, Artur Osyczka, Brian A Zabel and Joanna Cichy
    Academic press:
    Journal of Biological Chemistry (rok: 2019, tom: 294, strony: 1267-1278), Wydawca: American Society for Biochemistry and Molecular Biology Privacy Policy
    Status:
    Published
    DOI:
    10.1074/jbc.RA118.005495 - link to the publication
  23. Identication, Characterization, and Tissue Expression Pattern of Alternatively Spliced Transcript Variants of Mouse RARRES2 Gene
    Authors:
    Kamila Kwiecien, Pawel Majewski, Maciej Bak, Piotr Brzoza, Urszula Godlewska, Izabella Skulimowska, Joanna Cichy, Mateusz Kwitniewski
    Academic press:
    Scientific Reports (rok: 2021, ), Wydawca: Springer Nature
    Status:
    Submitted
    DOI:
    10.21203/rs.3.rs-371498/v1 - link to the publication
  24. Antibiograms in five pipetting steps: precise dilution assays in sub-microliter volumes with a conventional pipette
    Authors:
    L. Derzsi, T.S. Kaminski and P. Garstecki
    Academic press:
    Lab on a Chip (rok: 2016, tom: 16, strony: 893-901), Wydawca: ROYAL SOC CHEMISTRY
    Status:
    Published
    DOI:
    10.1039/C5LC01151E - link to the publication
  25. Chemerin-derived peptide Val66-Pro85 is effective in limiting methicillin-resistant S. aureus skin infection.
    Authors:
    Aneta Zegar, Urszula Godlewska, Dorota Kozłowska-Chmielewska, Pawel Majewski, Brian A Zabel, Joanna Cichy
    Academic press:
    Frontiers in Microbiology (rok: 2021, tom: 12, strony: 45299), Wydawca: Frontiers Media SA
    Status:
    Published
    DOI:
    10.3389/fmicb.2021.742610 - link to the publication
  26. High-Throughput Monitoring of Bacterial Cell Density in Nanoliter Droplets: Label-Free Detection of Unmodified Gram-Positive and Gram-Negative Bacteria
    Authors:
    Natalia Pacocha, Jakub Bogusławski, Michał Horka, Karol Makuch,Kamil Liżewski, Maciej Wojtkowski, Piotr Garsteck
    Academic press:
    Analytical Chemistr (rok: 2021, tom: 93, 2, strony: 843–850), Wydawca: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/acs.analchem.0c03408 - link to the publication
  27. Non-wetting droplets in capillaries of circular cross-section: scaling function
    Authors:
    Karol Makuch, Jean-Baptiste Gorce, and Piotr Garstecki
    Academic press:
    Physics of Fluids (rok: 2019, tom: 31, strony: 43102), Wydawca: AIP Publishing
    Status:
    Published
    DOI:
    10.1063/1.5087722 - link to the publication
  28. Antimicrobial and attractant roles for chemerin in the oral cavity during inflammatory gum disease
    Authors:
    U.Godlewska, P.Brzoza, A.Sroka, P.Majewski, H.Jentsch, M.Eckert, S.Eick, J.Potempa, B.Zabel and J.Cichy
    Academic press:
    Frontiers in Immunology (rok: 2017, tom: 0,578472222222222, strony: 45302), Wydawca: Frontiers
    Status:
    Published
    DOI:
    10.3389/fimmu.2017.00353 - link to the publication
  29. Architecture of antimicrobial skin defense.
    Authors:
    Kwiecien K, Zegar A, Jung J, Brzoza P, Kwitniewski M, Godlewska U, Grygier B, Kwiecinska P, Morytko A, Cichy J.
    Academic press:
    Cytokine & Growth Factor Reviews (rok: 2019, tom: 49, strony: 70-84), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.cytogfr.2019.08.001 - link to the publication
  30. Droplet-based digital antibiotic susceptibility screen reveals single-cell clonal heteroresistance in an isogenic bacterial population
    Authors:
    Ott Scheler, Karol Makuch, Pawel R Debski, Michal Horka, Artur Ruszczak, Natalia Pacocha, Krzysztof Sozanski, Olli-Pekka Smolander, Witold Postek, Piotr Garstecki
    Academic press:
    Scientific Reports (rok: 2020, tom: 10, strony: 3282), Wydawca: Springer Nature
    Status:
    Published
    DOI:
    10.1038/s41598-020-60381-z - link to the publication
  31. Gravity-driven microfluidic assay for digital enumeration of bacteria and for antibiotic susceptibility testing
    Authors:
    Yu-Ting Kao, Tomasz S. Kaminski, Witold Postek, Jan Guzowski, Karol Makuch, Artur Ruszczak, Felix von Stetten, Roland Zengerle, Piotr Garstecki
    Academic press:
    Lab on a Chip (rok: 2020, tom: 20, strony: 54-63), Wydawca: The Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/C9LC00684B - link to the publication
  32. Microfluidic screening of antibiotic susceptibility at a single-cell level shows inoculum effect of cefotaxime in E. coli
    Authors:
    Witold Postek, Pawel Gargulinski, Ott Scheler, Tomasz S. Kaminski and Piotr Garstecki
    Academic press:
    Lab on a Chip (rok: 2018, tom: 18, strony: 3668–3677), Wydawca: Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/C8LC00916C - link to the publication
  33. Optimized droplet digital CFU assay (ddCFU) provides precise quantification of bacteria over dynamic range of 6 logs and beyond
    Authors:
    O. Scheler, N. Pacocha, P. R. Debski, A. Ruszczak, T. S. Kaminski and P. Garstecki
    Academic press:
    Lab on a Chip (rok: 2017, tom: 17, strony: 1980 - 1987), Wydawca: ROYAL SOC CHEMISTRY
    Status:
    Published
    DOI:
    10.1039/c7lc00206h - link to the publication

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