Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

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The influence of PERK-eIF2alpha pathway on the leukemia microenvironment; the role of cell-cell interactions and composition of exosomal miRNAs.

2013/10/E/NZ3/00673

Keywords:

CML,BCR-ABL1 exosomes miRNA PERK eIF2alpha microinvironment

Descriptors:

  • NZ3_1: Cell biology
  • NZ3_7: Signal transduction
  • NZ3_3: Apoptosis

Panel:

NZ3 - Cellular and developmental biology: cell biology, developmental biology, ageing biology, neurobiology

Host institution :

Instytut Biologii Doświadczalnej im. M. Nenckiego PAN

woj. mazowieckie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr hab. Katarzyna Piwocka 

Number of co-investigators in the project: 8

Call: SONATA BIS 3 - announced on 2013-06-14

Amount awarded: 1 500 000 PLN

Project start date (Y-m-d): 2014-06-10

Project end date (Y-m-d): 2019-06-09

Project duration:: 60 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. System do transferu kwasów nukleinowych typu Northern (piec hybryzyzacyjny ProBlot oraz aparat do transferu pod ciśnieniem i zasilaczem) (40 000 PLN)
  2. Luminometr GloMax 20/20 (40 000 PLN)

Information in the final report

  • Publication in academic press/journals (8)
  • Articles in post-conference publications (21)
  • Book publications / chapters in book publications (1)
  1. Characteristics of live parameters of the HS-5 human bone marrow stromal cell line cocultured with the leukemia cells in hypoxia, for the studies of leukemia-stroma cross-talk
    Authors:
    Podszywalow-Bartnicka P, Kominek A, Wolczyk M, Kolba MD, Swatler J, Piwocka K.
    Academic press:
    Cytometry, Part A (rok: 2018, tom: 93(9), strony: 929-940), Wydawca: ISAC/Wiley
    Status:
    Published
    DOI:
    10.1002/cyto.a.23580 - link to the publication
  2. Chronic myeloid leukemia-derived extracellular vesicles increase Foxp3 level and suppressive activity of thymic regulatory T cells
    Authors:
    Swatler J., Dudka W., Bugajski L, Kozlowska E & Piwocka K
    Academic press:
    European Journal of Immunology (rok: 2020, tom: 50(4), strony: 606-609), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/eji.201848051 - link to the publication
  3. Increased phosphorylation of eIF2α in chronic myeloid leukemia cells stimulates secretion of matrix modifying enzymes.
    Authors:
    Podszywalow-Bartnicka P, Cmoch A, Wolczyk M, Bugajski L, Tkaczyk M, Dadlez M, Nieborowska-Skorska M, Koromilas AE, Skorski T, Piwocka K
    Academic press:
    Oncotarget (rok: 2016, tom: 7 (48), strony: 79706-79721), Wydawca: Oncotarget
    Status:
    Published
    DOI:
    10.18632/oncotarget.12941 - link to the publication
  4. Isolation and Characterization of Extracellular Vesicles from Cell Culture Conditioned Medium for Immunological Studies
    Authors:
    Swatler J., Dudka W., Piwocka K
    Academic press:
    Current Protocols in Immunology (rok: 2020, tom: 29(1), strony: e96), Wydawca: Willey
    Status:
    Published
    DOI:
    10.1002/cpim.96 - link to the publication
  5. Tunneling nanotube-mediated intercellular vesicle and protein transfer in the stroma-provided imatinib resistance in chronic myeloid leukemia cells.
    Authors:
    Kolba MD, Dudka W, Zaręba-Kozioł M, Kominek A, Ronchi P, Turos L, Chroscicki P, Wlodarczyk J, Schwab Y, Klejman A, Cysewski D, Srpan K, Davis DM, Piwocka K.
    Academic press:
    Cell Death Disease (rok: 2019, tom: 10(11):817, strony: 817), Wydawca: Nature
    Status:
    Published
    DOI:
    10.1038/s41419-019-2045-8 - link to the publication
  6. Tunneling nanotubes contribute to the stroma-mediated imatinib resistance of leukemic cells
    Authors:
    Marta D. Kolba, Wioleta Dudka, Monika Zareba-Koziol, Agata Kominek, Paolo Ronchi, Laura Turos, Jakub Wlodarczyk, Yannick Schwab, Dominik Cysewski, Katja Srpan, Daniel M. Davis, Katarzyna Piwocka
    Academic press:
    bioRXiv Preprint (rok: 2018, tom: -, strony: 20090), Wydawca: Cold Spring Harbor Laboratory
    Status:
    Published
    DOI:
    10.1101/425041 - link to the publication
  7. Egzosomalne mikroRNA jako element komunikacji międzykomórkowej w nowotworach.
    Authors:
    Wesolowska A, Piwocka K.
    Academic press:
    Postepy Biochemii (rok: 2017, tom: 63, nr 2/2017, strony: 110-118), Wydawca: Polskie Towarzystwo Biochemiczne
    Status:
    Published
    DOI:
    brak - link to the publication
  8. Stress granules assembly affects detection of mRNA in living cells by the NanoFlares; an important aspect of the technology
    Authors:
    Wolczyk M, Podszywalow-Bartnicka P, Bugajski L, Piwocka K.
    Academic press:
    Biochimica et Biophysica Acta (BBA) - General Subjects (rok: 2017, tom: 1861(5), strony: 1024-1035), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10. - link to the publication
  1. CML-derived extracellular vesicles increase Foxp3 level and suppressive activity of thymic regulatory T cells (Outstanding Poster Award)
    Authors:
    Julian Swatler,Wioleta Dudka-Ruszkowska, Lukasz Bugajski, Ewa Kozłowska, Katarzyna Piwocka
    Conference:
    33rd Congress of the International Society for Advancement of Cytometry (rok: 2018, ), Wydawca: International Society for Advancement of Cytometry (ISAC)
    Data:
    konferencja 28.04-2.05.2018, Praga, Rep. Czeska
    Status:
    Published
  2. ISR activation in Chronic Myeloid Leukemia cells modifies composition of microRNAs secreted in extracellular vesicles
    Authors:
    Agnieszka Wesolowska, Paulina Podszywalow-Bartnicka, Lukasz Bugajski, Konrad Debski, Michal Dabrowski, Katarzyna Piwocka
    Conference:
    8th International Conference On Tumor Microenvironment: Progression, Therapy & Prevention (rok: 2018, ), Wydawca: The International Cancer Microenvironment Society
    Data:
    konferencja 10-14.06.2018, Lizbona, Portugalia
    Status:
    Published
  3. Stress response signaling in leukemia cells modifies profile of released proteases to support invasion.
    Authors:
    Paulina Podszywalow-Bartnicka, Anna Cmoch, Magdalena Wolczyk, Marta Tkaczyk, Michal Dadlez, Tomasz Skorski, Katarzyna Piwocka
    Conference:
    EMBO Symposium "Tumour Microenvironment and Signalling" (rok: 2016, ), Wydawca: EMBO
    Data:
    konferencja 3-6.04.2016, Heidelberg, Niemcy
    Status:
    Published
  4. Stress response signaling in leukemia cells modifies profile of secreted proteases.
    Authors:
    Paulina Podszywalow-Bartnicka, Anna Cmoch, Agnieszka Wesolowska, Lukasz Bugajski, Katarzyna Piwocka
    Conference:
    ISEV 2016 (rok: 2016, ), Wydawca: ISEV
    Data:
    konferencja 4-7.05.2016, Rotterdam, Holandia
    Status:
    Published
  5. Tunneling Nanotubes (TNTs) mediate bidirectional transfer of specific vesicular cargo and proteins between leukemia and stroma cells
    Authors:
    Marta D. Kolba, Monika Zareba-Koziol, Wioleta Dudka-Ruszkowska, Agata Kominek, Katja Srpan Jakub Wlodarczyk, Daniel M. Davis and Katarzyna Piwocka
    Conference:
    ASCB / EMBO 2017 Meeting (rok: 2017, ), Wydawca: ASCB & EMBO
    Data:
    konferencja 2-6.12 2017
    Status:
    Published
  6. Activation of stress-induced signaling in CML promotes release of factors stimulating invasiveness of bone marrow stroma fibroblasts
    Authors:
    Paulina Podszywalow-Bartnicka, Anna Cmoch, Agnieszka Wesolowska, Lukasz Bugajski, Marta Tkaczyk, Slawomir Pikula, Michal Dadlez, Tomasz Skorski, Katarzyna Piwocka
    Conference:
    International Conference on The Tumour Microenvironment in the Haematological Malignancies and its Therapeutic Targeting (rok: 2015, ), Wydawca: ESH
    Data:
    konferencja 7-9.05.2015, Lisbon, Portugalia
    Status:
    Published
  7. BCR-ABL-containing leukemic extracellular vesicles contribute to immunosuppression in chronic myeloid leukemia by controlling Foxp3 and regulatory T cells biology
    Authors:
    Julian Swatler, Lukasz Bugajski, Wioleta Dudka, Laura Turos, Ewa Kozlowska, Katarzyna Piwocka
    Conference:
    34th Congress of the International Society for Advancement of Cytometry Cyto2019 (rok: 2019, ), Wydawca: ISAC
    Data:
    konferencja 22-26.06.2019
    Status:
    Published
    DOI:
    link to the publication
  8. Extracellular vesicles limit invasive potential of fibroblasts in anautocrine mechanism mediated by EVs microRNA
    Authors:
    Paulina Podszywalow-Bartnicka; Agnieszka Wesolowska; Anna Cmoch;Lukasz Bugajski; Katarzyna Piwocka
    Conference:
    ISEV2018 (rok: 2018, ), Wydawca: ISEV
    Data:
    konferencja 2-6.05.2018
    Status:
    Published
    DOI:
    link to the publication
  9. Influence of CML-derived extracellular vesicles on differentiation and function of thymic regulatory T cells
    Authors:
    Julian Swatler, Wioleta Dudka-Ruszkowska, Paulina Podszywałow-Bartnicka, Ewa Kozłowska & Katarzyna Piwocka
    Conference:
    19th International Summer School on Immunology (rok: 2017, ), Wydawca: FEBS
    Data:
    konferencja 23-30.09.2017, Hvar, Chorwacja
    Status:
    Published
  10. Regulation of Foxp3 level and suppressive activity of thymic regulatory T cells by CML-derived extracellular vesicles
    Authors:
    J. Swatler, W. Dudka-Ruszkowska, L. Bugajski, E. Kozlowska, K. Piwocka
    Conference:
    European Congress of Immunology (rok: 2018, ), Wydawca: European Society of Immunology
    Data:
    konferencja 2-5.09.2018
    Status:
    Published
    DOI:
    link to the publication
  11. Structure and function of Tunneling Nanotubes – direct cel-cell connections formed between leukemia and stroma cells.
    Authors:
    Katarzyna Piwocka, Marta Walczak, Agata Kowalczyk, Wioleta Dudka-Ruszkowska, Paulina Podszywalow-Bartnicka, Piotr Sunderland, Jakub Włodarczyk
    Conference:
    31st Congress of the International Society for Advancement of Cytometry Cyto2016 (rok: 2016, ), Wydawca: ISAC
    Data:
    konferencja 11-15.06.2016, Seattle, USA (oral presentation)
    Status:
    Published
  12. Integrated stress response and ATF4 transcription factor as regulators of microRNAs secreted by leukemia cells
    Authors:
    Agnieszka Wesolowska, Paulina Podszywalow- Bartnicka, Magdalena Wolczyk, Michal Dabrowski, Katarzyna Piwocka
    Conference:
    The Non-Coding Genome (EMBO/EMBL Symposium) (rok: 2017, ), Wydawca: EMBO
    Data:
    konferencja 13-16.09.2017, Heidelber, Niemcy
    Status:
    Published
  13. Stress-induced signaling in chronic myeloid leukemia promotes cells invasiveness
    Authors:
    Paulina Podszywalow-Bartnicka, Anna Cmoch, Agnieszka Wesolowska, Lukasz Bugajski, Marta Tkaczyk, Michal Dadlez, Tomasz Skorski, Katarzyna Piwocka
    Conference:
    8th International Conference On Tumor Microenvironment: Progression, Therapy & Prevention (rok: 2018, ), Wydawca: The International Cancer Microenvironment Society
    Data:
    konferencja 10-14.06.2018, Lizbona, Portugalia (oral presentation)
    Status:
    Published
  14. Targeting Integrated Stress Response Combined with Imatinib As a Novel Strategy to Eradicate Imatinib-Resistant CML Cells
    Authors:
    Wioleta Dudka, Grazyna Hoser, Monika Kusio-Kobialka, Julian Swatler, Laura Turos-Korgul, Agata Kominek, Marta Brewinska-Olchowik, Milena Wiech, Paulina Podszywalow-Bartnicka, Agata Klejman, Tomasz Stoklosa, Antonis E. Koromilas and Katarzyna Piwocka
    Conference:
    22nd John Goldman E-Conference on CML (rok: 2020, ), Wydawca: ESH
    Data:
    konferencja 1-4.10.2020
    Status:
    Published
  15. Targeting the eIF2alpha phosphorylation shows therapeutic potential for chronic myeloid leukemia.
    Authors:
    Wioleta Dudka-Ruszkowska, Paulina Podszywalow-Bartnicka, Monika Kusio-Kobialka, Magdalena Wolczyk, Grazyna Hoser, Michal Dabrowski, Marta Tkaczyk, Michal Dadlez, Antonis E. Koromilas and Katarzyna Piwocka
    Conference:
    17th Annual John Goldman Conference on Chronic Myeloid Leukemia: Biology and Therapy (rok: 2015, ), Wydawca: ESH
    Data:
    konferencja 1-4.10.2015, Estoril, Portugalia
    Status:
    Published
  16. Novel insights into the leukemia microenvironment; role of the unfolded protein response.
    Authors:
    Katarzyna Piwocka, Paulina Podszywalow-Bartnicka, Marta Walczak, Agata Kowalczyk, Lukasz Bugajski, Anna Cmoch, Jakub Wlodarczyk
    Conference:
    32nd Congress of the International Society for Advancement of Cytometry Cyto2017 (rok: 2017, ), Wydawca: ISAC
    Data:
    konferencja 10-14.06.2017, Boston, USA (oral presentation)
    Status:
    Published
  17. Novel role of BCR-ABL-containing leukemic extracellular vesicles in controlling the function of regulatory T cells
    Authors:
    Swatler J., Dudka-Ruszkowska W., Bugajski L., Kozłowska E., Piwocka K.
    Conference:
    ISEV (International Society for Extracellular Vesicles) 2019 Annual Meeting (rok: 2019, ), Wydawca: ISEV
    Data:
    konferencja 24-28.04.2019, Kyoto, Japan
    Status:
    Published
  18. Functional and proteomic insight into modulation of Foxp3+ regulatory T cells by leukemic extracellular vesicles
    Authors:
    Julian Swatler, Laura Turos, Dominik Cysewski, Marta Brewinska-Olchowik, Ewa Kozlowska, Paulina Pilanc, Katarzyna Piwocka
    Conference:
    CYTO Virtual 2020 (rok: 2020, ), Wydawca: ISAC
    Data:
    konferencja 4-5.08.2020
    Status:
    Published
    DOI:
    link to the publication
  19. Not only gene mutations matter: Incorporation of flow cytometry into the strategy to identify BRCA1-deficient leukemias susceptible to synthetic lethality by PARP1 inhibitors (oral presentation)
    Authors:
    Katarzyna Piwocka, Paulina Podszywalow-Bartnicka, Margaret Nieborowska-Skorska, Magdalena Wolczyk, Lukasz Bugajski, Tomasz Skorski
    Conference:
    33rd Congress of the International Society for Advancement of Cytometry (rok: 2018, ), Wydawca: International Society for Advancement of Cytometry (ISAC)
    Data:
    konferencja 28.04-2.05.2018, Praga, Rep. Czeska (oral presentation)
    Status:
    Published
  20. Tunneling Nanotubes (TNTs) mediate bidirectional transfer of specific vesicular cargo and proteins between leukemia and stroma cells
    Authors:
    Marta D. Kolba, Wioleta Dudka-Ruszkowska, Monika Zareba-Koziol, Agata Kominek, Jakub Wlodarczyk, Daniel M. Davis, Katarzyna Piwocka
    Conference:
    8th International Conference On Tumor Microenvironment: Progression, Therapy & Prevention (rok: 2018, ), Wydawca: The International Cancer Microenvironment Society
    Data:
    konferencja 10-14.06.2018, Lizbona, Portugalia (oral presentation)
    Status:
    Published
  21. Tunneling nanotube-mediated vesicle and protein transfer contributes to the stroma-mediated imatinib resistance of leukemic cells
    Authors:
    Marta D. Kolba, Wioleta Dudka, Monika Zaręba-Kozioł, Agata Kominek, Paolo Ronchi, Laura Turos, Jakub Wlodarczyk, Yannick Schwab, Dominik Cysewski, Katja Srpan, Daniel M. Davis, Katarzyna Piwocka
    Conference:
    3rd ESH Scientific Workshop on Haematological Tumour Mircoenvironment and Its Therapeutic Targeting (rok: 2019, ), Wydawca: ESH
    Data:
    konferencja 24-26.02.2019, London
    Status:
    Published
  1. Insight into the Leukemia Microenvironment and Cell-cell Interactions Using Flow Cytometry
    Authors:
    Katarzyna Piwocka, Paulina Podszywalow-Bartnicka, Julian Swatler, Marta D. Kolba, Agata Kominek, Ewa Kozlowska
    Book:
    Multidimensional Flow Cytometry Techniques for Novel Highly Informative Assays (rok: 2018, tom: 2, strony: 19-42), Wydawca: InTech
    Status:
    Published
    DOI:
    link to the publication

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