Projects funded by the NCN


Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

Delete all

The role of microparticles in pathomechanisms of diabetic retinopathy - analysis of intercellular communication mechanisms in endothelial aging

2012/07/B/NZ5/02510

Keywords:

microparticles endothelium diabetic retinopathy miRNA vascula ageing

Descriptors:

  • NZ5_3: Pathogenesis of human diseases
  • NZ3_4: Ageing
  • NZ4_4: Pathophysiology

Panel:

NZ5 - Human and animal noninfectious diseases: etiology, mechanisms, diagnosis and treatment of diseases, poisonings and injuries (without neurological diseases)

Host institution :

Uniwersytet Jagielloński-Collegium Medicum, Collegium Medicum, Wydział Lekarski

woj. małopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr hab. Ewa Stępień 

Number of co-investigators in the project: 7

Call: OPUS 4 - announced on 2012-09-15

Amount awarded: 697 100 PLN

Project start date (Y-m-d): 2013-06-26

Project end date (Y-m-d): 2017-06-25

Project duration:: 48 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. czytnik absorbancji, fluorescencji i luminescencji z oprogramowaniem do pomiarów kinetycznych, spektralnych i analizy odczytów (90 000 PLN)

Information in the final report

  • Publication in academic press/journals (10)
  • Articles in post-conference publications (7)
  1. Microparticles in endothelial function
    Authors:
    Ewa Stępień, Marta Targosz-Korecka
    Academic press:
    Postępy Biochemii (rok: 2013, tom: 59, strony: 395-404), Wydawca: Kwartalnik Polskiego Towarzystwa Biochemicznego
    Status:
    Published
  2. Circulating Microvesicles in Regenerative Angiogenesis
    Authors:
    Drożdż A, Stępień E
    Academic press:
    Curr Trends in Biomed Eng & Bioscien (rok: 2017, tom: 3;1, strony: 001-003), Wydawca: JP Juniper Publishers
    Status:
    Published
    DOI:
    10.19080/CTBEB.2017.03.555602 - link to the publication
  3. The effects of subdiffusion on the NTA size measurements of extracellular vesicles in biological samples
    Authors:
    M Majka, M Durak-Kozica, A Kamińska, A Opalińska, M Szczęch, E Stępień
    Academic press:
    arXiv.org>physics>biological physics (rok: 2018, tom: 1701,09001, strony: 45309), Wydawca: Cornell University Library
    Status:
    Published
  4. Urinary Extracellular Vesicles: Potential Biomarkers of Renal Function in Diabetic Patients
    Authors:
    Kamińska A, Platt M, Kasprzyk J, Kuśnierz-Cabala B, Gala-Błądzińska A, Woźnicka O, Jany BR, Krok F, Piekoszewski W, Kuźniewski M, Stępień EŁ
    Academic press:
    J Diabetes Res (rok: 2016, tom: 2016, strony: 5741518), Wydawca: Hindawi
    Status:
    Published
    DOI:
    10.1155/2016/5741518 - link to the publication
  5. Application of nano-LC-MALDI-TOF/TOF-MS for proteomic analysis of microvesicles.
    Authors:
    Kasprzyk J, Stępień E, Piekoszewski W.
    Academic press:
    Clin Biochem (rok: 2017, tom: 50, strony: 241-243), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.clinbiochem.2016.11.013 - link to the publication
  6. Circulating ectosomes – targeting angiogenic microRNAs in diabetes
    Authors:
    Stępień E, Durak-Kozica M, Kamińska A, Targosz-Korecka M, Libera M, Tylko G Opalińska A, Kapusta M, Solnica B, Georgescu A, Costa M, Czyżewska-Buczyńska A, Witkiewicz W, Małecki M, Enguita FJ
    Academic press:
    Theranostics (rok: 2018, tom: 8, strony: 3874-3890), Wydawca: Ivyspring International Publisher Pty Ltd
    Status:
    Published
    DOI:
    10.7150/thno.23334 - link to the publication
  7. Extracellular vesicles participate in the transport of cytokines and angiogenic factors in diabetic patients with ocular complications.
    Authors:
    Tokarz A, Szuścik I, Kuśnierz-Cabala B, Kapusta M, Konkolewska M, Żurakowski A, Georgescu A, Stępień E.
    Academic press:
    Folia Med Cracov (rok: 2015, tom: 55, strony: 35-48), Wydawca: PAN
    Status:
    Published
  8. The role of microparticles in pathomechanisms of diabetic retinopathy – analysis of intercellular communication mechanisms in endothelial aging. Case control study in patients with metabolic syndrome, diabetes type 1 and type 2
    Authors:
    Ewa Stępień, Iwona Szuścik, Aleksandra Tokarz, Francisco J. Enguita, Bogdan Solnica, Aleksander Żurakowski, Maciej Małecki
    Academic press:
    Journal of Medical Science (rok: 2014, tom: 83, strony: 322-327), Wydawca: Poznan University of Medical Sciences
    Status:
    Published
  9. Raman spectral signatures of urinary extracellular vesicles from diabetic patients and hyperglycemic endothelial cells as potential biomarkers in diabetes
    Authors:
    Maciej Roman, Agnieszka Kamińska, Anna Drożdż, Mark Platt, Marek Kuźniewski, Maciej T Małecki, Wojciech Kwiatek, Czesława Paluszkiewicz, Ewa Ł Stępień
    Academic press:
    anotechnology, Biology, and Medicine , Wydawca: Elsevier
    Status:
    Submitted
  10. Immobilization and detection of platelet-derived extracellular vesicles on functionalized silicon substrate: cytometric and spectrometric approach
    Authors:
    Gajos K, Kamińska A, Awsiuk K, Bajor A, Gruszczyński K, Pawlak A, Żądło A, Kowalik A, Budkowski A, Stępień E
    Academic press:
    Download PDF Analytical and Bioanalytical Chemistry (rok: 2017, tom: 409, strony: 1109–1119), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s00216-016-0036-5 - link to the publication
  1. Hyperglycemia induced microvesicles control endothelial cell migration.
    Authors:
    Drożdż AE, Jach R, Huras H, STĘPIEŃ E.
    Conference:
    ISEV 2017 International meeting, Toronto, Canada (rok: 2017, ), Wydawca: Taylor&Francis, Journal of Extracellular Vesicles
    Data:
    konferencja 18-21 May
    Status:
    Published
  2. Simplified method for proteome analysis of platelet-induced membrane vesicles
    Authors:
    Joanna Kasprzyk, Wojciech Piekoszewski, Ewa Stepien
    Conference:
    The Fifth International Meeting of ISEV (International Society of Extracellular Vesicles) (rok: 2016, ), Wydawca: Taylor & Francis Online
    Data:
    konferencja 4-7 maja
    Status:
    Published
  3. Human plasma microparticles are specific source of pro- and antiangiogenic miRNAs
    Authors:
    Ewa L. Stepien, Iwona Wybranska, Roman Pfitzner and Francisco J. Enguita
    Conference:
    ISEV 2014 Annual Meeting (rok: 2014, ), Wydawca: ISEV
    Data:
    konferencja 30.04-03.05
    Status:
    Published
  4. Interplay of RANTES chemokine and CCR5+ bearing microvesicles in diabetic retinopathy.
    Authors:
    Tokarz A, Drożdż AE, Szuścik I, STEPIEŃ E.
    Conference:
    ISEV 2017 International meeting, Toronto, Canada (rok: 2017, ), Wydawca: Taylor&Francis; Journal of Extracellular Vesicles
    Data:
    konferencja 18-21 May
    Status:
    Published
  5. Urinary extracellular vesicles number and diameter as biomarkers of renal disease in diabetic patients
    Authors:
    Agnieszka Kaminska, Mark Platt, Beata Kusnierz-Cabala, Marek Kuzniewski, Ewa Stepien
    Conference:
    The Fifth International Meeting of ISEV (International Society for Extracellular Vesicles) (rok: 2016, ), Wydawca: Taylor & Francis Online
    Data:
    konferencja 4-7 Maja
    Status:
    Published
  6. Circulating Tie2+ microvesicles as potential indicators of diabetic retinopathy progression.
    Authors:
    Tokarz A, Drożdż AE, Szuścik I, STEPIEŃ E.
    Conference:
    ISEV 2017 International meeting, Toronto, Canada (rok: 2017, ), Wydawca: Taylor&Francis, Journal of Extracellular Vesicles
    Data:
    konferencja 18-21 May
    Status:
    Published
  7. Improved criteria of MVs size discrimination allow better characteristics of patients with controlled and uncontrolled diabetes mellitus.
    Authors:
    M. Durak, K. Rawojć, A.Tokarz, A. Opalińska, W. Łojkowski, E.L. Stepien.
    Conference:
    ISEV 2015 meeting. Washington DC (rok: 2015, ), Wydawca: Journal of Extracellular Vesicles 2015, 4: 27783
    Data:
    konferencja 23-26 April 2015
    Status:
    Published