Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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The mechanism of regioselective oxidation of cholesterol derivatives by a novel molybdenum enzyme, steroid 25-OH dehydrogenase from Stereolibacterium denitrificans

2012/05/D/ST4/00277

Keywords:

molybdenum enzyme enzymatic catalysis hydrocarbon oxidation DFT calculations

Descriptors:

  • ST4_13: Solid-state chemistry, surface physicochemistry
  • ST4_15: Colloid chemistry
  • NZ1_2: Biochemistry

Panel:

ST4 - Chemistry: physical chemistry/chemical physics, theoretical chemistry, analytical chemistry, inorganic chemistry, organic chemistry, method development

Host institution :

Instytut Katalizy i Fizykochemii Powierzchni im. Jerzego Habera PAN

woj. małopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr hab. Maciej Szaleniec 

Number of co-investigators in the project: 4

Call: SONATA 3 - announced on 2012-03-15

Amount awarded: 397 515 PLN

Project start date (Y-m-d): 2013-02-11

Project end date (Y-m-d): 2017-07-10

Project duration:: 53 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Wysokoobrotowa wirówka preparatywna z rotorami.
  2. Wytrząsarka kołyskowa.
  3. zasilacz UPS (6 000 PLN)
  4. UPS (dla LC-MS).
  5. Zawór do formowania gradientu.
  6. Komputer stacjonarny (3 500 PLN)
  7. Maszyna do lodu płatkowego.
  8. System klimatyzacji glove-boxa.
  9. Beztlenowa komora rękawicowa z winylu typy glove box (100 000 PLN)
  10. System kolumn do oczyszczania FPLC (20 000 PLN)
  11. Termocykler (12 000 PLN)
  12. Termomikser.
  13. Uniwersalna wirówka z rotorem na próbki o objętości 50ml (20 000 PLN)
  14. Upgrade serwera obliczeniowego.
  15. Chiller do komory rękawicowej.
  16. Źródło jonów APCI.

Information in the final report

  • Publication in academic press/journals (6)
  • Articles in post-conference publications (19)
  1. Ethylbenzene Dehydrogenase and Related Molybdenum Enzymes Involved in Oxygen-Independent Alkyl Chain Hydroxylation
    Authors:
    J. Heider, M. Szaleniec, K. Sünwoldt , M. Boll
    Academic press:
    J Mol Microbiol Biotechnol (rok: 2016, tom: 26, strony: 45-62), Wydawca: KARGER
    Status:
    Published
    DOI:
    10.1159/000441357 - link to the publication
  2. Regioselective hydroxylation of cholecalciferol, cholesterol and other sterol derivatives by steroid C25 dehydrogenase
    Authors:
    A. Rugor, M. Tataruch, J. Staroń, A. Dudzik, E. Niedziałkowska, P. Nowak, A. Hogendorf, A. Zym, D. B.Napruszewska, A. Jarzębski, K. Szymańska, W. Białas, M. Szaleniec
    Academic press:
    Appl Microbiol Biotechnol (rok: 2017, tom: 101, strony: 1163-1174), Wydawca: SPRINGER
    Status:
    Published
    DOI:
    10.1007/s00253-016-7880-2 - link to the publication
  3. Electrocatalytic Hydroxylation of Sterols by Steroid C25 Dehydrogenase from Sterolibacterium denitrificans
    Authors:
    Palraj Kalimuthu, Agnieszka M. Wojtkiewicz, Maciej Szaleniec, Paul V. Bernhardt
    Academic press:
    Chemistry A European Journal (rok: 2018, tom: 24, strony: 7710-7717), Wydawca: ChemPubSoc Europe
    Status:
    Published
    DOI:
    10.1002/chem.201800616. - link to the publication
  4. Reaction mechanism of sterol hydroxylation by steroid C25 dehydrogenase – homology model, reactivity and isoenzymatic diversity
    Authors:
    A. Rugor, M. Tataruch, J. Staroń, A. Dudzik, E. Niedziałkowska, P. Nowak, A. Hogendorf, A. Zym, D. B.Napruszewska, A. Jarzębski, K. Szymańska, W. Białas, M. Szaleniec
    Academic press:
    Appl Microbiol Biotechnol (rok: 2017, tom: 173, strony: 28–43), Wydawca: SPRINGER
    Status:
    Published
    DOI:
    10.1016/j.jinorgbio.2017.04.027 - link to the publication
  5. Bacterial steroid hydroxylases: enzyme classes, their functions and comparison of their catalytic mechanisms
    Authors:
    Maciej Szaleniec, Agnieszka M. Wojtkiewicz, Rita Bernhardt, Tomasz Borowski, Marina Donova
    Academic press:
    Applied Microbiology and Biotechnology (rok: 2018, tom: 102, strony: 8153–8171), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s00253-018-9239-3 - link to the publication
  6. Optimization of overexpression of a chaperone protein of steroid C25 dehydrogenase for biochemical and biophysical characterization
    Authors:
    Ewa Niedzialkowska, Beata Mrugała, Agnieszka Rugor, Mateusz P. Czub, Anna Skotnicka, Julien J. H. Cotelesage, Graham N. George, Maciej Szaleniec, Wladek Minor, Krzysztof Lewiński
    Academic press:
    Protein Expression and Purification (rok: 2017, tom: 134, strony: 47-62), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.pep.2017.03.019 - link to the publication
  1. Bacterial dehydrogenases from S. denitrificans - biocatalytic tool for modification of sterols and steroids
    Authors:
    M. Szaleniec, A. Dudzik, E. Niedziałkowska, A. Rugor, M. Tataruch
    Conference:
    Workshop on Agricultural Biotechnology and Biocatalysis (rok: 2015, ), Wydawca: NCBiR
    Data:
    konferencja 03.12.2015
    Status:
    Published
  2. Reaction mechanism of sterol hydroxylation by steroid C25 dehydrogenase: homology model, reactivity and isoenzymatic diversity
    Authors:
    1.A. Rugor, A. Wójcik-Augustyn, E. Niedzialkowska, S. Mordalski,J. Staroń, A. Bojarski, M. Szaleniec
    Conference:
    XLIX Ogólnopolskie Kolokwium Katalityczne (rok: 2017, ), Wydawca: IKiFP PAN
    Data:
    konferencja 15-17.03.2017
    Status:
    Published
  3. Role of molybdenum in hetero, homo, and enzymatic catalysis: selected examples studied by theory approach
    Authors:
    M. Witko, R. Tokarz-Sobieraj, D. Rutkowska-Żbik, M. Szaleniec, R, Grybos.
    Conference:
    Europacat 2017 (rok: 2017, ), Wydawca: European Research Institute of Catalysis
    Data:
    konferencja 27-31.08.2017
    Status:
    Published
  4. The mechanism of regioselective hydroxylation of sterols by steroid C25 dehydrogenase
    Authors:
    A. Rugor, A. Wójcik, S. Mordalski, M. Szaleniec
    Conference:
    16th International Conference on Theoretical Aspects of Catalysis (rok: 2016, ), Wydawca: Jerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences
    Data:
    konferencja 19-23.06
    Status:
    Published
  5. Molybdenum oxidoreductases - regio- and enantioselective hydroxylation of organic compounds
    Authors:
    Maciej Szaleniec, Agnieszka Rugor, Mateusz Tataruch, Johann Heider
    Conference:
    8th World Congress on Oxidation Catalysis (rok: 2017, ), Wydawca: IKiFP PAN
    Data:
    konferencja 3-8.09.2017
    Status:
    Published
  6. Steroid C25 Dehydrogenase, the Bocatalyst for Production of Calcifediol and 25-Hydroxycholesterol
    Authors:
    Maciej Szaleniec, Agnieszka Rugor and Anna Wójcik
    Conference:
    3rd Symposium on Biotransformations for Pharmaceutical and Cosmetic Industry (rok: 2016, ), Wydawca: Institute of Organic Chemistry, PAS
    Data:
    konferencja 30.06-2.07
    Status:
    Published
  7. The Mechanism of Regioselective Hydroxylation of Sterols by Steroid C25 Dehydrogenase
    Authors:
    A. Rugor, A. Wójcik M. Szaleniec
    Conference:
    8th International Congress on Biocatalysis Biocat (rok: 2016, ), Wydawca: TuTECH Hamburg
    Data:
    konferencja 28.08-1.09
    Status:
    Published
  8. Application Potential of steroid C25 Dehydrogenase from Sterolibacterium Denitrificans
    Authors:
    A. Rugor, A. Dudzik, M. Tataruch, J. Staroń, A. Bojarski, M. Szaleniec
    Conference:
    7th International Congress on Biocatalysis (rok: 2014, ), Wydawca: TuTech Verlag
    Data:
    konferencja 08-04.09.2014
    Status:
    Published
  9. Badania nad strukturą i aktywnością katalityczną Dehydrogenazy C25 steroidowej z Sterolibacterium Denitrificans
    Authors:
    A. Rugor, A. Dudzik, N. Zawada, S. Mordalski, J. Staroń, A. Bojarski, M. Szaleniec
    Conference:
    XLVI Ogólnopolskie Kolokwium Katalityczne (rok: 2014, ), Wydawca: IKiFP PAN
    Data:
    konferencja 19-21.03.2014
    Status:
    Published
  10. Homology model, reactivity and isoenzymatic diversity of steroid C25 dehydrogenase, a molybdoenzyme hydroxylating sterols and vitamin D3
    Authors:
    Agnieszka Rugor, Anna Wójcik-Augustyn, Ewa Niedzialkowska, Maciej Szaleniec
    Conference:
    10th Molybdenym and Tungstate Enzyme Conference (rok: 2017, ), Wydawca: Brynmawr College
    Data:
    konferencja 18-23.06.2017
    Status:
    Published
  11. Selective modifications of steroids and sterols by enzymes from bacterial cholesterol degradation pathway
    Authors:
    M. Szaleniec , A. Rugor, T. Janeczko, M. Hochołowski, M. Guzik
    Conference:
    17th European Biotechnology Congress, (rok: 2016, ), Wydawca: New Biotechnology, ELSEVIER
    Data:
    konferencja 3-6.06
    Status:
    Published
    DOI:
    link to the publication
  12. Structural modelling of a novel molybdoenzyme: steroid C25 dehydrogenase from Sterolibacterium denitrificans
    Authors:
    A. Rugor, A. Wójcik, S. Mordalski, A. Bojarski, M. Szaleniec
    Conference:
    Modeling & Design of Molecular Materials 2014 (rok: 2014, ), Wydawca: Politechnika Wrocławska
    Data:
    konferencja 29.06-3.07.2014
    Status:
    Published
  13. The propinquity effect in enzyme catalysis: a case study of steroid C25 dehydrogenase
    Authors:
    A. Rugor, A. Wójcik, S. Mordalski, J. Staroń, A. Bojarski, M. Szaleniec
    Conference:
    XLVIII Ogólnopolskie Kolokwium Katalityczne (rok: 2016, ), Wydawca: IKiFP PAN
    Data:
    konferencja 16-18.03
    Status:
    Published
  14. Insight into structure and reactivity of steroid C25 dehydrogenase, a molybdenum enzyme hydroxylating sterols
    Authors:
    A. M. Rugor, A. Wójcik, S. Mordalski, J. Staroń, A. Bojarski, M. Szaleniec
    Conference:
    IX Molybdenum and Tungsten Enzymes Conference 2015 (rok: 2015, ), Wydawca: University of Cologne
    Data:
    konferencja 6-11.09.2015
    Status:
    Published
  15. MD and QM:MM studies of steroid C25 dehydrogenase catalytic subunit
    Authors:
    A. Rugor, A. Wójcik, S. Mordalski, A. Bojarski, M. Szaleniec
    Conference:
    XLVII Ogólnopolskie Kolokwium Katalityczne (rok: 2015, ), Wydawca: IKiFP PAN
    Data:
    konferencja 16-18 marca 2015
    Status:
    Published
  16. Reaction mechanism of sterol hydroxylation by steroid C25 dehydrogenase
    Authors:
    Agnieszka Rugor, Anna Wójcik-Augustyn, Stefan Mordalski, Maciej Szaleniec
    Conference:
    10th Molybdenym and Tungstate Enzyme Conference, (rok: 2017, ), Wydawca: Brynmawr College
    Data:
    konferencja 18-23.06.2017
    Status:
    Published
  17. Structure, Reactivity and Application of Steroid C25 Dehydrogenase to Hydroxylating Sterols and Vitamin D3
    Authors:
    A. Rugor, M. Tataruch, J. Staroń, M. Guzik, M. Szaleniec
    Conference:
    8th International Congress on Biocatalysis Biocat (rok: 2016, ), Wydawca: TuTECH Hamburg
    Data:
    konferencja 28.08-1.09
    Status:
    Published
  18. Reaction mechanism of regioselective sterol hydroxylation catalyzed by steroid C25 dehydrogenase: quantum chemical study
    Authors:
    A. Rugor, A. Wójcik, M. Szaleniec
    Conference:
    XLVIII Ogólnopolskie Kolokwium Katalityczne (rok: 2016, ), Wydawca: IKiFP PAN
    Data:
    konferencja 16-18.03
    Status:
    Published
  19. Steroid C25 dehydrogenase – the mechanism of regioselective hydroxylation of sterols and cholecalciferol
    Authors:
    M. Szaleniec, A. Rugor , A. Wójcik
    Conference:
    17th European Biotechnology Congress (rok: 2016, ), Wydawca: New Biotechnology, Elsevier
    Data:
    konferencja 3-6.06
    Status:
    Published
    DOI:
    link to the publication

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