Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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New reagents and methods in synthesis of modified nucleotides and their application for preparation biochemical tools and nucleotides with great therapeutic potential

2012/05/E/ST5/03893

Keywords:

nucleotides synthesis 5' mRNA cap click reactions modified oligophosphates synthesis protein biosynthesis

Descriptors:

  • ST5_16:
  • ST5_11: Smart materials – self assembled materials, external stimuli-responsive materials
  • NZ1_2: Biochemistry

Panel:

ST5 - Materials: materials synthesis, structure-properties relations, advanced and functional materials with designed properties, (macro)molecular architecture, material engineering

Host institution :

Uniwersytet Warszawski, Centrum Nowych Technologii Uniwersytetu Warszawskiego

woj. mazowieckie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr hab. Jacek Jemielity 

Number of co-investigators in the project: 6

Call: SONATA BIS 1 - announced on 2012-03-15

Amount awarded: 1 479 000 PLN

Project start date (Y-m-d): 2013-03-15

Project end date (Y-m-d): 2018-03-14

Project duration:: 60 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. HPLC - pompa poczwórna, 600 barów, detektory UV-Vis, fluorescencyjny, autosampler (400 000 PLN)

Information in the final report

  • Publication in academic press/journals (17)
  • Book publications / chapters in book publications (1)
  1. Analysis of Decapping Scavenger (DcpS)-cap complex using modified cap analogs reveals molecular determinants for efficient cap binding
    Authors:
    Anna Wypijewska del Nogal, Marius D. Surleac, Joanna Kowalska, Maciej Lukaszewicz, Jacek Jemielity, Martin Bisaillon, Edward Darzynkiewicz, Adina L. Milac, Elzbieta Bojarska
    Academic press:
    FEBS Journal (rok: 2013, tom: 280, strony: 6508-6527), Wydawca: WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
    Status:
    Published
    DOI:
    10.1111/febs.12553 - link to the publication
  2. Five eIF4E isoforms from Arabidopsis thaliana are characterized by distinct features of cap analogs binding
    Authors:
    Anna Kropiwnicka, Krzysztof Kuchta, Maciej Lukaszewicz, Joanna Kowalska, Jacek Jemielity, Krzysztof Ginalski, Edward Darzynkiewicz, Joanna Zuberek
    Academic press:
    Biochemical and Biophysical Research Communications (rok: 2015, tom: 456, strony: 47-52), Wydawca: Elsevier Inc.
    Status:
    Published
    DOI:
    10.1016/j.bbrc.2014.11.032 - link to the publication
  3. Potential therapeutic applications of RNA cap analogs
    Authors:
    Marcin Ziemniak, Malwina Strenkowska, Joanna Kowalska, Jacek Jemielity
    Academic press:
    Future Medicinal Chemistry (rok: 2013, tom: 5, strony: 1141-1172), Wydawca: FUTURE SCI LTD, UNITED HOUSE, 2 ALBERT PL, LONDON, N3 1QB, ENGLAND
    Status:
    Published
    DOI:
    10.4155/fmc.13.96) - link to the publication
  4. Roquin targets mRNAs in a 3′-UTR-specific manner by different modes of regulation
    Authors:
    Katharina Essig, Nina Kronbeck, Joao C. Guimaraes, Claudia Lohs, Andreas Schlundt, Anne Hoffmann, Gesine Behrens, Sven Brenner, Joanna Kowalska, Cristina Lopez-Rodriguez, Jacek Jemielity, Helmut Holtmann, Kristin Reiche, Jörg Hackermüller, Michael Sattler, Mihaela Zavolan , Vigo Heissmeyer
    Academic press:
    Nature Communications (rok: 2018, tom: 9, strony: 3810), Wydawca: NATURE PUBLISHING GROUP, MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND
    Status:
    Published
    DOI:
    10.1038/s41467-018-06184-3 - link to the publication
  5. Synthesis and evaluation of fluorescent cap analogues for mRNA labelling
    Authors:
    Marcin Ziemniak, Mariusz Szabelski, Maciej Lukaszewicz, Anna Nowicka, Edward Darzynkiewicz, Zbigniew Wieczorek, Robert E. Rhoads, Jacek Jemielity
    Academic press:
    RSC Advances (rok: 2013, tom: 3, strony: 20943-20958), Wydawca: ROYAL SOC CHEMISTRY, THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND
    Status:
    Published
    DOI:
    10.1039/c3ra42769b - link to the publication
  6. Synthesis, properties, and biological activity of boranophosphate analogs of the mRNA cap: versatile tools for manipulation of therapeutically relevant cap-dependent processes
    Authors:
    Joanna Kowalska, Anna Wypijewska del Nogal, Zbigniew M. Darzynkiewicz, Janina Buck, Corina Nicola, Andreas N. Kuhn, Maciej Lukaszewicz, Joanna Zuberek, Malwina Strenkowska, Marcin Ziemniak, Maciej Maciejczyk, Elzbieta Bojarska, Robert E. Rhoads, Edward Darzynkiewicz, Ugur Sahin and Jacek Jemielity
    Academic press:
    Nucleic Acids Research (rok: 2014, tom: 42, strony: 10245–10264), Wydawca: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/nar/gku757 - link to the publication
  7. Cap analogs modified with 1,2-dithiodiphosphate moiety protect mRNA from decapping and enhance its translational potential
    Authors:
    Malwina Strenkowska, Renata Grzela, Maciej Majewski, Katarzyna Wnek, Joanna Kowalska, Maciej Lukaszewicz, Joanna Zuberek, Edward Darzynkiewicz, Andreas N Kuhn, Ugur Sahin, Jacek Jemielity
    Academic press:
    Nucleic Acids Research (rok: 2016, tom: 44, strony: 9578-9590), Wydawca: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/nar/gkw896 - link to the publication
  8. Fluorescent HTS assay for phosphohydrolases based on nucleoside 5ʹ-fluorophosphates: application in screening for inhibitors of mRNA Decapping Scavenger and PDE-I
    Authors:
    Marek R. Baranowski, Anna Nowicka, Jacek Jemielity, Joanna Kowalska
    Academic press:
    Organic & Biomolecular Chemistry (rok: 2016, tom: 14, strony: 4595-4604), Wydawca: The Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/c6ob00492j - link to the publication
  9. Synthesis of Fluorophosphate Nucleotide Analogues and Their Characterization as Tools for 19F NMR Studies
    Authors:
    Marek R. Baranowski, Anna Nowicka, Anna M. Rydzik, Marcin Warmiński, Renata Kasprzyk, Błażej A. Wojtczak, Jacek Wójcik, Timothy D. W. Claridge, Joanna Kowalska, Jacek Jemielity
    Academic press:
    The Journal of Organic Chemistry (rok: 2015, tom: 80, strony: 3982-3997), Wydawca: AMER CHEMICAL SOC
    Status:
    Published
    DOI:
    10.1021/acs,joc.5b00337 - link to the publication
  10. A novel route for preparing 5′ cap mimics and capped RNAs: phosphate-modified cap analogues obtained via click chemistry
    Authors:
    Sylwia Walczak, Anna Nowicka, Dorota Kubacka, Kaja Fac, Przemyslaw Wanat, Seweryn Mroczek, Joanna Kowalska, Jacek Jemielity
    Academic press:
    Chemical Science (rok: 2017, tom: 8, strony: 260-267), Wydawca: The Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/c6sc02437h - link to the publication
  11. Clickable trimethylguanosine cap analogs modified within the triphosphate bridge: synthesis, conjugation to RNA and susceptibility to degradation
    Authors:
    Blazej A. Wojtczak, Marcin Warminski, Joanna Kowalska, Malgorzata Honcharenko, C. I. Edvard Smith, Roger Stromberg, Edward Darzynkiewicz, Jacek Jemielity
    Academic press:
    RSC Advances (rok: 2016, tom: 6, strony: 8317-8328), Wydawca: ROYAL SOC CHEMISTRY
    Status:
    Published
    DOI:
    10.1039/c5ra25684d - link to the publication
  12. Ethynyl, 2-Propynyl, and 3-Butynyl C-Phosphonate Analogues of Nucleoside Di- and Triphosphates: Synthesis and Reactivity in CuAAC
    Authors:
    Przemyslaw Wanat, Sylwia Walczak, Blazej A. Wojtczak, Monika Nowakowska, Jacek Jemielity, Joanna Kowalska
    Academic press:
    Organic Letters (rok: 2015, tom: 17, strony: 3062-3065), Wydawca: AMER CHEMICAL SOC
    Status:
    Published
    DOI:
    10.1021/acs.orglett.5b01346 - link to the publication
  13. Structural basis of mRNA-cap recognition by Dcp1–Dcp2
    Authors:
    Jeffrey S. Mugridge, Marcin Ziemniak, Jacek Jemielity, John D. Gross
    Academic press:
    Nature Structural & Molecular Biology (rok: 2016, tom: 23, strony: 987-994), Wydawca: NATURE PUBLISHING GROUP
    Status:
    Published
    DOI:
    10.1038/nsmb.3301 - link to the publication
  14. mRNA cap analogues substituted in the tetraphosphate chain with CX2: identification of O-to-CCl2 as the first bridging modification that confers resistance to decapping without impairing translation
    Authors:
    Anna M. Rydzik, Marcin Warminski, Pawel J. Sikorski, Marek R. Baranowski, Sylwia Walczak, Joanna Kowalska, Joanna Zuberek, Maciej Lukaszewicz, Elzbieta Nowak, Timothy D. W. Claridge, Edward Darzynkiewicz, Marcin Nowotny, Jacek Jemielity
    Academic press:
    Nucleic Acids Research (rok: 2017, tom: 45, strony: 8661-8675), Wydawca: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/nar/gkx569 - link to the publication
  15. Cap analogs containing 6-thioguanosine – reagents for the synthesis of mRNAs selectively photo-crosslinkable with cap-binding biomolecules
    Authors:
    Monika Nowakowska, Joanna Kowalska, Franck Martin, Arnaud d'Orchymont, Joanna Zuberek, Maciej Lukaszewicz, Edward Darzynkiewicza and Jacek Jemielity
    Academic press:
    Org. Biomol. Chem. (rok: 2014, tom: 12, strony: 4841–4847), Wydawca: The Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/c4ob00059e - link to the publication
  16. Synthetic m3G-CAP attachment necessitates a minimum trinucleotide constituent to be recognised as a nuclear import signal
    Authors:
    Malgorzata Honcharenko, Burcu Bestas, Martina Jezowska, Blazej A Wojtczak, Pedro Moreno, Joanna Romanowska, Susanna M Bächle, Edward Darzynkiewicz, Jacek Jemielity, C. I. Edvard Smith, Roger Stromberg
    Academic press:
    RSC Advances (rok: 2016, tom: 6, strony: 51367-51373), Wydawca: The Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/C6RA09568B - link to the publication
  17. The synthesis of isopropylidene mRNA cap analogs modified with phosphorothioate moiety and their evaluation as promoters of mRNA translation
    Authors:
    Marcin Warminski, Joanna Kowalska, Janina Buck, Joanna Zuberek, Maciej Lukaszewicz, Corina Nicola, Andreas N. Kuhn , Ugur Sahin, Edward Darzynkiewicz, Jacek Jemielity
    Academic press:
    Bioorganic & Medicinal Chemistry Letters (rok: 2013, tom: 23, strony: 3753-3758), Wydawca: PERGAMON-ELSEVIER SCIENCE LTD, THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND
    Status:
    Published
    DOI:
    10.1016/j.bmcl.2013.05.001 - link to the publication
  1. Synthetic Capped mRNAs for Cap-Specific Photo-Cross-Linking Experiments
    Authors:
    Joanna Kowalska, Franck Martin, Jacek Jemielity
    Book:
    Synthetic mRNA: Production, Introduction Into Cells, and Physiological Consequences (rok: 2016, tom: 1428, strony: 31-43), Wydawca: Springer Science+Business Media
    Status:
    Published

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