Projects funded by the NCN


Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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The influence of metabolic pathway inhibitors on the development and quality of bovine embryos produced in vitro.

2012/05/B/NZ9/03349

Keywords:

inhibitors signalling pathways embryo pluripotency cattle

Descriptors:

  • NZ3_13: Developmental biology in animals
  • NZ3_8: Stem cell biology
  • NZ3_1: Cell biology

Panel:

NZ9 - Fundamentals of applied life sciences and biotechnology: agricultural, forestry, horticulture, animal production and fishery, food and nutrition sciences, industrial biosciences, environmental biotechnology and remediation

Host institution :

Uniwersytet Przyrodniczy w Poznaniu, Wydział Medycyny Weterynaryjnej i Nauk o Zwierzętach

woj. wielkopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr hab. Dorota Cieślak 

Number of co-investigators in the project: 5

Call: OPUS 3 - announced on 2012-03-15

Amount awarded: 671 596 PLN

Project start date (Y-m-d): 2013-03-25

Project end date (Y-m-d): 2017-03-24

Project duration:: 48 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Miernik pH S220-Kit.
  2. Wirówka Cytologiczna Cytospin 4, Elektro Med (45 000 PLN)
  3. Mieszadło hotMix1 z zestawem akcesoriów.
  4. Doposażenie mikroskopu Leica AM6000.
  5. Wirówka z chłodzeniem (0stC) Eppendorf 5418R (24 000 PLN)
  6. Analityczna waga laboratoryjna (10 000 PLN)
  7. kamera z monitorem zewnętrzym (dodatkowe wyposażenie istniejacego stereoskopowego mikroskopu Olympus SZX-7) (9 000 PLN)

Information in the final report

  • Publication in academic press/journals (2)
  • Articles in post-conference publications (4)
  1. Inhibitor mediated WNT and MEK/ERK signalling affects apoptosis and the expression of quality related genes in bovine in vitro obtained blastocysts
    Authors:
    Madeja ZE, Warzych E, Pawlak P, Lechniak D.
    Academic press:
    Biochem Biophys Res Commun (rok: 2019, tom: 510 (3), strony: 403-408), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bbrc.2019.01.113 - link to the publication
  2. WNT signalling supported by MEK/ERK inhibition is essential to maintain pluripotency in bovine preimplantation embryo
    Authors:
    Ewelina Warzych, Piotr Pawlak, Dorota Lechniak, Zofia Eliza Madeja
    Academic press:
    Developmental Biology , Wydawca: Elsevier
    Status:
    Accepted for publication
    DOI:
    10.1016/j.ydbio.2020.04.004. - link to the publication
  1. The effect of 2i inhibitor system on pluripotency marker genes expression in bovine blastocysts.
    Authors:
    Hryniewicz K., Madeja Z., Lechniak D.
    Conference:
    The 2014 Regenerative Medicine Event: Stem Cell Reprogramming (rok: 2014, ), Wydawca: Euroscicon
    Data:
    konferencja 02.06.2014
    Status:
    Published
  2. Quality of bovine blastocysts produced in vitro with the 3i/2i inhibitor systems
    Authors:
    Warzych Ewelina, Madeja Zofia, Pawlak Piotr, Lechniak-Cieslak Dorota
    Conference:
    Epigenetic and Periconcept Environment (rok: 2016, ), Wydawca: COST
    Data:
    konferencja 26-29.09.2016
    Status:
    Published
  3. Genetic factors regulating cell lineage specification and pluripotency maintenance at the early stage of bovine embryonic development
    Authors:
    Madeja ZE, Pawlak P, Warzych-Plejer E, Cieślak D
    Conference:
    Polski Kongres Genetyki (rok: 2016, ), Wydawca: Polskie Towarzystwo Genetyczne
    Data:
    konferencja 19-22.06.2016
    Status:
    Published
  4. Wpływ inhibitorów ścieżek różnicowania komórkowego (system 2i) na jakość blastocyst bydła - indeks apoptotyczny i całkowita liczba blastomerów.
    Authors:
    Hryniewicz K., Warzych E., Madeja Z.E., Perkowska A., Lechniak-Cieślak D.
    Conference:
    VII Zjazd Towarzystwa Biologii Rozrodu (rok: 2014, ), Wydawca: Towarzystwo Biologii Rozrodu
    Data:
    konferencja 11-13.09.2014
    Status:
    Published