Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

Delete all

DYNAMICS OF CHROMOSOME ARCHITECTURE AND REPLICATION DURING DIFFERENT BACTERIAL GROWTH PHASES - comparision of two related actinomycetales: pathogenic Mycobacterium and non-pathogenic mycelial Streptomyces.

2012/04/A/NZ1/00057

Keywords:

Actinomycetales Mycobacterium Streptomyces chromosomes replication chromosome architecture time-lapse microfluidic-microscopy (TLMM)

Descriptors:

  • NZ1_1: Molecular biology
  • NZ1_10: Microbiology

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

UNIWERSYTET WROCŁAWSKI, Wydział Biotechnologii

woj. dolnośląskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Jolanta Zakrzewska-Czerwińska 

Number of co-investigators in the project: 10

Call: MAESTRO 2 - announced on 2011-12-15

Amount awarded: 2 942 410 PLN

Project start date (Y-m-d): 2012-08-31

Project end date (Y-m-d): 2018-06-30

Project duration:: 70 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Oprogramowanie SoftWoRx wraz z jednostka sterująca do mikroskopu fluorescencyjnego..
  2. mikroskop fluorescencyjny z komorą inkubacyjną (420 000 PLN)
  3. Wytrząsarka (40 000 PLN)
  4. Automatyczny czytnik wzrostu drobnoustrojów - Bioscreen (135 000 PLN)

Information in the final report

  • Publication in academic press/journals (15)
  1. Choreography of the Mycobacterium replication machinery during the cell cycle
    Authors:
    Trojanowski D, Ginda K, Pióro M, Hołówka J, Skut P, Jakimowicz D, Zakrzewska-Czerwińska J.
    Academic press:
    mBio (rok: 2015, tom: 6, strony: 45303), Wydawca: American Society of Microbiology
    Status:
    Published
    DOI:
    10.1128/mBio.02125-14 - link to the publication
  2. The studies of ParA and ParB dynamics reveal asymmetry of chromosome segregation in mycobacteria.
    Authors:
    Ginda K, Santi I, Bousbaine D, Zakrzewska-Czerwińska J, Jakimowicz D, McKinney J.
    Academic press:
    Mol Microbiol. (rok: 2017, tom: 105, strony: 453-468), Wydawca: John Wiley & Sons
    Status:
    Published
    DOI:
    10.1111/mmi.13712 - link to the publication
  3. Where and When Bacterial Chromosome Replication Starts: A Single Cell Perspective
    Authors:
    Trojanowski D, Hołówka J, Zakrzewska-Czerwińska J.
    Academic press:
    Front Microbiol. (rok: 2018, tom: 9, strony: 2819), Wydawca: Frontiers Media SA
    Status:
    Published
    DOI:
    10.3389/fmicb.2018.02819 - link to the publication
  4. Multifork chromosome replication in slow-growing bacteria
    Authors:
    Trojanowski D, Hołówka J, Ginda K, Jakimowicz D, Zakrzewska-Czerwińska J
    Academic press:
    Sci Rep (rok: 2017, tom: 7, strony: 43836), Wydawca: Nature Publishing Group
    Status:
    Published
    DOI:
    10.1038/srep43836 - link to the publication
  5. oriC-encoded instructions for the initiation of bacterial chromosome replication
    Authors:
    Wolański M, Donczew R, Zawilak-Pawlik A, Zakrzewska-Czerwińska J.
    Academic press:
    Front Microbiol. (rok: 2015, tom: 5, strony: 45305), Wydawca: Frontiers
    Status:
    Published
    DOI:
    10.3389/fmicb.2014.00735 - link to the publication
  6. Fifty years after the replicon hypothesis: cell-specific master regulators as new players in chromosome replication contro
    Authors:
    Marcin Wolański, Dagmara Jakimowicz, Jolanta Zakrzewska-Czerwińska
    Academic press:
    Journal of Bacteriology (rok: 2014, tom: 196, strony: 2901-2911), Wydawca: American Society of Microbiology
    Status:
    Published
    DOI:
    10.1128/JB.01706-14 - link to the publication
  7. Initiation of Chromosomal Replication in Predatory Bacterium Bdellovibrio bacteriovorus.
    Authors:
    Makowski Ł, Donczew R, Weigel C, Zawilak-Pawlik A, Zakrzewska-Czerwińska J
    Academic press:
    Front Microbiol. (rok: 2016, tom: 7, strony: 45304), Wydawca: Frontiers Media SA
    Status:
    Published
    DOI:
    10.3389/fmicb.2016.01898 - link to the publication
  8. The Origin of Chromosomal Replication Is Asymmetrically Positioned on the Mycobacterial Nucleoid, and the Timing of Its Firing Depends on HupB.
    Authors:
    Hołówka J, Trojanowski D, Janczak M, Jakimowicz D, Zakrzewska-Czerwińska J.
    Academic press:
    J. Bacteriol (rok: 2018, tom: 10, strony: 45303), Wydawca: ASM
    Status:
    Published
    DOI:
    10.1128/JB.00044-18 - link to the publication
  9. Watching DNA Replication Inhibitors in Action: Exploiting Time-Lapse Microfluidic Microscopy as a Tool for Target-Drug Interaction Studies in Mycobacterium
    Authors:
    Damian Trojanowski, Marta Kołodziej, Joanna Hołówka, Jolanta Zakrzewska-Czerwińska
    Academic press:
    Antimicrob Agents Chemother. (rok: 2019, tom: 63, strony: e00739-19), Wydawca: ASM
    Status:
    Published
    DOI:
    10.1128/AAC.00739-19 - link to the publication
  10. Amsacrine Derivatives Selectively Inhibit Mycobacterial Topoisomerase I (TopA), Impair M. smegmatis Growth and Disturb Chromosome Replication
    Authors:
    Szafran MJ, Kołodziej M, Skut P, Medapi B, Domagała A, Trojanowski D, Zakrzewska-Czerwińska J, Sriram D, Jakimowicz D
    Academic press:
    Front Microbiol. (rok: 2018, tom: 9, strony: 1592), Wydawca: Frontiers Media SA
    Status:
    Published
    DOI:
    10.3389/fmicb.2018.01592 - link to the publication
  11. HupB Is a Bacterial Nucleoid-Associated Protein with an Indispensable Eukaryotic-Like Tail.
    Authors:
    Hołówka J, Trojanowski D, Ginda K, Wojtaś B, Gielniewski B, Jakimowicz D, Zakrzewska-Czerwińska J.
    Academic press:
    mBio (rok: 2017, tom: 8, strony: 45308), Wydawca: ASM
    Status:
    Published
    DOI:
    10.1128/mBio.01272-17 - link to the publication
  12. Streptomycete origin of chromosomal replication with two putative unwinding elements.
    Authors:
    Płachetka M, Żyła-Uklejewicz D, Weigel C, Doncze R, Donczew M, Zawilak-Pawlik A, Zakrzewska-Czerwinska J
    Academic press:
    Microbiology (rok: 2019, tom: 165, strony: 1365-1375), Wydawca: Microbiology Society
    Status:
    Published
    DOI:
    10.1099/mic.0.000859 - link to the publication
  13. AfsK-Mediated Site-Specific Phosphorylation Regulates DnaA Initiator Protein Activity in Streptomyces coelicolor
    Authors:
    Łebkowski T, Wolański M, Ołdziej S, Flärdh K, Zakrzewska-Czerwińska J.
    Academic press:
    J Bacteriol. (rok: 2020, tom: 202, strony: e00597-19), Wydawca: ASM
    Status:
    Published
    DOI:
    10.1128/JB.00597-19 - link to the publication
  14. Competition between DivIVA and the nucleoid for ParA binding promotes segrosome separation and modulates mycobacterial cell elongation.
    Authors:
    Pióro M, Malecki T, Portas M, Magierowska I, Trojanowski D, Sherratt DJ , Zakrzewska-Czerwinska J , Ginda K , Jakimowicz D
    Academic press:
    Molecular Microbiology (rok: 2018, tom: 111, strony: 204-220), Wydawca: John Wiley & Sons
    Status:
    Published
    DOI:
    10.1111/mmi.14149 - link to the publication
  15. Unique Function of the Bacterial Chromosome Segregation Machinery in Apically Growing Streptomyces - Targeting the Chromosome to New Hyphal Tubes and its Anchorage at the Tips
    Authors:
    Kois-Ostrowska A1, Strzałka A, Lipietta N, Tilley E, Zakrzewska-Czerwińska J, Herron P, Jakimowicz D
    Academic press:
    PLoS Genet. (rok: 2016, tom: 12(12), strony: e1006488), Wydawca: PLoS
    Status:
    Published
    DOI:
    10.1371/journal.pgen.1006488 - link to the publication

O Narodowym Centrum Nauki

Finansowanie nauki

Współpraca zagraniczna

Centrum prasowe

Deklaracja dostępności
Przetwarzanie danych osobowych
Polityka cookies

Partnerzy