Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Cytotoxic conjugates targeting FGFR-related cancers based on FGF1 and FGFR-specific antibodies.

2011/02/A/NZ1/00066

Keywords:

fibroblast growth factor 1 fibroblast growth factor receptor cancer antibodies phage display cytotoxic conjugates

Descriptors:

  • NZ1_9: Biotechnology

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

Uniwersytet Wrocławski, Wydział Biotechnologii

woj. dolnośląskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Jacek Otlewski 

Number of co-investigators in the project: 14

Call: MAESTRO 1 - announced on 2011-06-15

Amount awarded: 2 796 000 PLN

Project start date (Y-m-d): 2012-04-30

Project end date (Y-m-d): 2017-08-30

Project duration:: 64 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Urządzenie do dokumentacji oraz analizy żeli i membran (80 000 PLN)
  2. Urządzenie do monitorowania w czasie rzeczywistym odpowiedzi komórkowej (180 000 PLN)
  3. Wielofunkcyjny czytnik mikropłytek (300 000 PLN)

Information in the final report

  • Publication in academic press/journals (10)
  • Articles in post-conference publications (4)
  1. Antibody-induced dimerization of FGFR1 promotes receptor endocytosis independently of its kinase activity
    Authors:
    Łukasz Opaliński, Aleksandra Sokołowska-Wędzina, Martyna Szczepara, Małgorzata Zakrzewska, Jacek Otlewski
    Academic press:
    Scientific Reports (rok: 2017, tom: 7, strony: 7121), Wydawca: Macmillan Publishers Limited
    Status:
    Published
    DOI:
    10.1038/s41598-017-07479-z - link to the publication
  2. Current methods for the synthesis of homogeneous antibody-drug conjugates
    Authors:
    Alicja M. Sochaj, Karolina W. Świderska and Jacek Otlewski
    Academic press:
    Biotechnology Advances (rok: 2015, tom: 33, strony: 775–784), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.biotechadv.2015.05.001 - link to the publication
  3. Design and In Vitro Evaluation of a Cytotoxic Conjugate Based on the Anti-HER2 Affibody Fused to the Fc Fragment of IgG1
    Authors:
    Alicja M. Sochaj-Gregorczyk, Patryk Ludzia, Emilia Kozdrowska, Piotr Jakimowicz, Aleksandra Sokolowska-Wedzina, Jacek Otlewski
    Academic press:
    International Journal of Molecular Sciences (rok: 2017, tom: 18, strony: E1688), Wydawca: MDPI AG
    Status:
    Published
    DOI:
    10.3390/ijms18081688 - link to the publication
  4. High-Affinity Internalizing Human scFv-Fc Antibody for Targeting FGFR1-Overexpressing Lung Cancer
    Authors:
    Aleksandra Sokolowska-Wedzina, Grzegorz Chodaczek, Julia Chudzian, Aleksandra Borek, Malgorzata Zakrzewska, Jacek Otlewski
    Academic press:
    Molecular Cancer Research (rok: 2017, tom: 8, strony: 1040-1050), Wydawca: American Association for Cancer Research
    Status:
    Published
    DOI:
    10.1158/1541-7786.MCR-16-0136 - link to the publication
  5. High-Yield Site-Specific Conjugation of Fibroblast Growth Factor 1 with Monomethylauristatin E via Cysteine Flanked by Basic Residues
    Authors:
    Michal Lobocki, Malgorzata Zakrzewska, Anna Szlachcic, Mateusz A. Krzyscik, Aleksandra Sokolowska-Wedzina, Jacek Otlewski
    Academic press:
    Bioconjugate Chemistry (rok: 2017, tom: 28, strony: 1850−1858), Wydawca: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/acs.bioconjchem.7b00158 - link to the publication
  6. Design and characteristics of cytotoxic fibroblast growth factor 1 conjugate for fibroblast growth factor receptor-targeted cancer therapy
    Authors:
    Anna Szlachcic, Malgorzata Zakrzewska, Michal Lobocki, Piotr Jakimowicz, Jacek Otlewski
    Academic press:
    Drug Des Devel Ther. (rok: 2016, tom: 10, strony: 2547—2560), Wydawca: Dove Medical Press
    Status:
    Published
    DOI:
    10.2147/DDDT.S105896 - link to the publication
  7. Generation of high-affinity, internalizing anti-FGFR2 single-chain variable antibody fragment fused with Fc for targeting gastrointestinal cancers
    Authors:
    Aleksandra Borek, Aleksandra Sokolowska-Wedzina, Grzegorz Chodaczek and Jacek Otlewski
    Academic press:
    PlosOne , Wydawca: Public Library of Science
    Status:
    Submitted
    DOI:
    brak - link to the publication
  8. The autoinhibitory function of D1 domain of FGFR1 goes beyond the inhibition of ligand binding
    Authors:
    Lukasz Opalinski, Martyna Szczepara, Aleksandra Sokolowska-Wedzina, Malgorzata Zakrzewska, Jacek Otlewski
    Academic press:
    International Journal of Biochemistry and Cell Biology (rok: 2017, tom: 89, strony: 193–198), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.biocel.2017.06.015 - link to the publication
  9. Efficient production and purification of extracellular domain of human FGFR-Fc fusion proteins from Chinese hamster ovary cells
    Authors:
    Aleksandra Sokołowska-Wędzina, Aleksandra Borek, Julia Chudzian, Piotr Jakimowicz, Małgorzata Zakrzewska, Jacek Otlewski
    Academic press:
    Protein Expression and Purification (rok: 2014, tom: 99, strony: 50-57), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.pep.2014.03.012. - link to the publication
  10. Protease Resistant Variants of FGF1 with Prolonged Biological Activity
    Authors:
    Agnieszka Kobielak, Malgorzata Zakrzewska, Michal Kostas, Piotr Jakimowicz, Jacek Otlewski and Daniel Krowarsch
    Academic press:
    Protein & Peptide Letters (rok: 2014, tom: 21, strony: 434-443), Wydawca: Bentham Science Publishers
    Status:
    Published
    DOI:
    10.2174/0929866520666131203102315 - link to the publication
  1. Effective in vitro affinity maturation of antiFGFR1 single chain variable fragment antibody
    Authors:
    A. Sokolowska-Wedzina, A. Borek, J. Otlewski
    Conference:
    IX Międzynarodowe Studenckie Sympozjum Naukowe "Między Biotechnologią, a Ochroną Środowiska" (rok: 2014, ), Wydawca: Biologist Scientific Union of University of Zielona Góra
    Data:
    konferencja 6-8.11
    Status:
    Published
  2. Phage Display selection and characterization of scFv specifically recognizing extracellular domain of FGFR1
    Authors:
    Jakub Szymczyk, Julia Chudzian, Łukasz Opaliński, Martyna Szczepara, Małgorzata Zakrzewska, Jacek Otlewski
    Conference:
    EUROBIOTECH, Central European Congress of Life Sciences Eurobiotech (rok: 2017, ), Wydawca: Targi w Krakowie Ltd
    Data:
    konferencja 11-14.09
    Status:
    Published
  3. Selection and characterization of human antibody fragments specific for FGFR2 - a cancer associated protein
    Authors:
    A. Borek, A. SokolowskaWedzina, J. Otlewski
    Conference:
    Empowered Antibodies Congress: Recombinant Antibodies & Bispecifics (rok: 2014, ), Wydawca: Informa Life Sciences
    Data:
    konferencja 18-19.06
    Status:
    Published
  4. Selection of high-affinity human monoclonal antibody fragments specific to the FGFR1 - a cancer-associated protein
    Authors:
    A. Sokolowska-Wedzina, A. Borek, J. Otlewski
    Conference:
    Empowered Antibodies Congress: Antibody Drug Conjugates (rok: 2014, ), Wydawca: Informa Life Sciences
    Data:
    konferencja 18-19.06
    Status:
    Published

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