Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

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Biochemical, structural and functional characterization of the plant exosome complex, using Arabidopsis thaliana as a model organism

2011/01/D/NZ1/03510

Keywords:

exosome RNA degradation RNases exoribonuclease endoribonuclease phosphorolytic activity

Descriptors:

  • NZ1_1: Molecular biology
  • NZ1_2: Biochemistry

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

Instytut Biochemii i Biofizyki PAN

woj. mazowieckie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr Rafał Tomecki 

Number of co-investigators in the project: 8

Call: SONATA 1 - announced on 2011-03-15

Amount awarded: 1 000 000 PLN

Project start date (Y-m-d): 2011-12-28

Project end date (Y-m-d): 2016-12-27

Project duration:: 60 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Wirówka z chłodzeniem (12 000 PLN)
  2. Aparaty do elektroforezy (2 szt.) (10 000 PLN)
  3. Pipety automatyczne, pipety elektroniczne, pipetory (18 szt.) (20 000 PLN)
  4. Kasety i ekrany do fosfoobrazowania (7 szt.) (28 000 PLN)
  5. Komercyjne kolumny chromatograficzne do sączenia molekularnego oraz chromatografii jonowymiennej (2 szt.) (20 000 PLN)
  6. Komputer (2 szt.) (10 000 PLN)

Information in the final report

  • Publication in academic press/journals (1)
  • Book publications / chapters in book publications (1)
  1. hUTP24 is essential for processing of the human rRNA precursor at site A1, but not at site A0
    Authors:
    Tomecki Rafał, Łabno Anna, Drążkowska Karolina, Cysewski Dominik, Dziembowski Andrzej
    Academic press:
    RNA Biology (rok: 2015, tom: 12(9), strony: 1010-1029), Wydawca: Taylor & Francis
    Status:
    Published
    DOI:
    10.1080/15476286.2015.1073437 - link to the publication
  1. Purification of eukaryotic exoribonucleases following heterologous expression in bacteria and analysis of their biochemical properties by in vitro enzymatic assays
    Authors:
    Tomecki Rafał, Drążkowska Karolina, Krawczyk Antonina, Kowalska Katarzyna, Dziembowski Andrzej
    Book:
    RNA remodelling proteins: methods and protocols (rok: 2015, tom: 1259, strony: 417-452), Wydawca: Springer
    Status:
    Published
    DOI:
    link to the publication

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