Protaminization of somatic cells nuclei as a model for nuclear reprogramming in somatic cell nuclear transfer.

2019/35/B/NZ3/02856

Keywords

Descriptors

  • NZ3_013: Biologia rozwoju zwierząt

Panel

NZ3 – Cellular and developmental biology: cell biology, developmental biology, ageing biology, neurobiology

Host institution

Instytut Genetyki i Hodowli Zwierząt PAN

Voivodeship: mazowieckie

Other projects carried out by the institution »

Principal investigator (from the host institution)

dr hab. Marta Czernik

Number of co-investigators in the project: 4

Call

OPUS 18 – announced on 2019-09-16

Amount awarded
1 738 800 PLN
Project start date
2020-07-28
Project end date
2025-07-27
Project duration
60 months (the same as in the proposal)
Project status
Project completed

Project description

Download the project description in a pdf file

Note — project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Information in the final report

Publication in academic press/journals (9)

  1. Omne vivum ex ovo: the oocyte reprogramming and remodeling activities.

    Authors:
    Helena Fulka, Pasqualino Loi, Marta Czernik, Azim Surani, Josef Fulka
    Academic press:
    Reproduction (year: 2023, volume: 165(3),, pages: pp. R75–R89), Publisher: Bioscientifica; BIOSCIENTIFICA LTD.; SOCIETY FOR REPRODUCTION AND FERTILITY
    Status:
    Published
    DOI:
    10.1530/REP-22-0124 – link to the publication
  2. Oocyte activation is a cytoplasm-confined event so far: what about the nucleus?

    Authors:
    Luisa Gioia, Luca Palazzese, Marta Czernik, Domenico Iuso, Helena Fulka, Josef Fulka Jr, and Pasqualino Loi
    Academic press:
    Reproduction (year: 2024, volume: 2024 Mar 1;, pages: 167(3): e230360), Publisher: Bioscientifica; BIOSCIENTIFICA LTD.; SOCIETY FOR REPRODUCTION AND FERTILITY
    Status:
    Published
    DOI:
    10.1530/REP-23-0360 – link to the publication
  3. Reviving vacuum-dried encapsulated ram spermatozoa via ICSI after 2 years of storage

    Authors:
    Luca Palazzese, Federica Turri, Debora Agata Anzalone, Joseph Saragusty, Jacques Bonnet, Marthe Colotte, Sophie Tuffet, Flavia Pizzi, Alessia Luciani, Kazutsugu Matsukawa, Marta Czernik, Pasqualino Loi
    Academic press:
    Frontiers in Veterinary Science (year: 2023, volume: 10, pages: 1270266), Publisher: Frontiers
    Status:
    Published
    DOI:
    10.3389/fvets.2023.1270266 – link to the publication
  4. 25th ANNIVERSARY OF CLONING BY SOMATIC-CELL NUCLEAR TRANSFER: Scientific and technological approaches to improve SCNT efficiency in farm animals and pets

    Authors:
    Loi P., Palazzese L., Scapolo P.A., Fulka J., Fulka H., Czernik M
    Academic press:
    Reproduction (year: 2021, volume: 162, pages: 33-43), Publisher: Society for Reproduction and Fertility
    Status:
    Published
    DOI:
    10.1530/REP-20-0653 – link to the publication
  5. Controlled spermatozoa-oocyte interaction improves embryo quality in sheep

    Authors:
    Anzalone D.A., Palazzese L., Czernik M., Sabatucci A., Valbonetti L., Capra E., Loi P
    Academic press:
    Scientific Reports (year: 2021, volume: 11, pages: 22692), Publisher: Nature
    Status:
    Published
    DOI:
    10.1038/s41598-021-02000-z – link to the publication
  6. Advances in induced anhydrobiosis for cell and gamete storage.

    Authors:
    Loi P, Palazzese L, Moncada M, Sterzo ML, Iuso D, Czernik M, Wakayama T, Kikawada T.
    Academic press:
    Trends Biotechnol (year: 2025, volume: 5:S0167-7799(25)00047-2, pages: 00047-2), Publisher: Cell Press
    Status:
    Published
    DOI:
    10.1016/j.tibtech.2025.02.010 – link to the publication
  7. Nucleus reprogramming/remodeling through selective enucleation (SE) of immature oocytes and zygotes: a nucleolus point of view

    Authors:
    Helena Fulka, Pasqualino Loi, Luca Palazzese, Michal Benc, Josef Fulka Jr Jr
    Academic press:
    The Journal of Reproduction and Development. (year: 2022, volume: 1;68(3):, pages: 165-172), Publisher: Society for Reproduction and Development
    Status:
    Published
    DOI:
    10.1262/jrd.2022-004 – link to the publication
  8. Exogenous LEA proteins expression enhances cold tolerance in mammalian cells by reducing oxidative stress

    Authors:
    Lo Sterzo M, Iuso D, Palazzese L, Moncada M, Boffa F, Scudieri A, Gioia L, Czernik M, Loi P.
    Academic press:
    Scientific Reports (year: 2025, volume: 15, pages: 3351), Publisher: Springer Nature
    Status:
    Published
    DOI:
    10.1038/s41598-025-86499-6 – link to the publication
  9. Mitochondrial function and intracellular distribution is severely affected in in vitro cultured mouse embryos

    Authors:
    Marta Czernik, Dawid Winiarczyk, Silvestre Sampino , Paweł Gręda , Salvatore Parillo, Jacek Andrzej Modliński, Pasqualino Loi
    Academic press:
    Scientific Reports (year: 2022, volume: 27;12(1), pages: 16152), Publisher: Nature Publishing Groupe
    Status:
    Published
    DOI:
    10.1038/s41598-022-20374-6 – link to the publication

Book publications / chapters in book publications (1)

  1. Somatic Cell Nuclear Transfer Using Freeze-Dried Protaminized Donor Nuclei

    Authors:
    Luca Palazzese, Marta Czernik, Kazutsugu Matsukawa, Pasqualino Loi
    Book:
    Somatic Cell Nuclear Transfer Technology (year: 2023, volume: 2647, pages: pp 211–224), Publisher: Humana Press
    Status:
    Published

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