Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

Delete all

Designing modified mRNAs as vaccines and therapeutic protein carriers. Studies on their immunogenicity in the context of translational activity and cellular stability.

2019/33/B/NZ1/01322

Keywords:

organic and bioorganic chemistry cap analogs mRNA RNA recognition mRNA degradation gene expression regulation translation control innate immune response IFIT and eIF4E proteins

Descriptors:

  • NZ1_003:
  • NZ1_001:

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

Uniwersytet Warszawski, Centrum Nowych Technologii Uniwersytetu Warszawskiego

woj. mazowieckie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr Renata Joanna Grzela 

Number of co-investigators in the project: 8

Call: OPUS 17 - announced on 2019-03-15

Amount awarded: 2 296 900 PLN

Project start date (Y-m-d): 2020-01-31

Project end date (Y-m-d): 2025-01-30

Project duration:: 60 months (the same as in the proposal)

Project status: Project completed

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Information in the final report

  • Publication in academic press/journals (9)
  1. Mesenchymal stem cell engineering by ARCA analog-capped mRNA
    Authors:
    Anna Andrzejewska, Renata Grzela, Anna Stankiewicz-Drogon, Piotr Rogujski, Siranjeevi Nagaraj, Edward Darzynkiewicz, Barbara Lukomska, Miroslaw Janowski
    Academic press:
    Molecular Therapy - Nucleic Acids (rok: 2023, tom: 33, strony: 454-468), Wydawca: CellPress
    Status:
    Published
    DOI:
    10.1016/j.omtn.2023.07.006 - link to the publication
  2. RNA sensor response in Hela cells for transfected mRNAs prepared in vitro by SP6 and HiT7 RNA polymerases: A comparative study.
    Authors:
    Nagaraj S., Stankiewicz-Drogoń A.,Darżynkiewicz E., Grzela R.
    Academic press:
    Frontiers in Bioengineering and Biotechnology (rok: 2022, tom: 10, strony: 45667), Wydawca: Frontiers
    Status:
    Published
    DOI:
    10.3389/fbioe.2022.1017934 - link to the publication
  3. The potential of N2-modified cap analogues for precise genetic manipulation through mRNA engineering
    Authors:
    Karol Kurpiejewski, Anna Stankiewicz-Drogon, Karolina Piecyk, Eliza Rajkowska, Paulina Skrzypczyk, Jingping Geng, Edward Darzynkiewicz, Renata Grzela, Marzena Jankowska-Anyszka
    Academic press:
    Frontiers in Molecular Biosciences (rok: 2024, tom: 10, strony: 1269028), Wydawca: Frontiers
    Status:
    Published
    DOI:
    10.3389/fmolb.2023.1269028 - link to the publication
  4. miR-483-5p orchestrates the initiation of protein synthesis by facilitating the decrease in phosphorylated Ser209eIF4E and 4E-BP1 levels
    Authors:
    Siranjeevi Nagaraj, Anna Stankiewicz-Drogon, Edward Darzynkiewicz, Urszula Wojda, Renata Grzela
    Academic press:
    Scientific Reports (rok: 2024, tom: 14, strony: 4237), Wydawca: Springer Nature
    Status:
    Published
    DOI:
    10.1038/s41598-024-54154-1 - link to the publication
  5. Insight into the Binding and Hydrolytic Preferences of hNudt16 Based on Nucleotide Diphosphate Substrates
    Authors:
    Chrabąszczewska M., Winiewska-Szajewska M., Ostrowska N., Bojarska E., Stępiński J., Mancewicz Ł., Łukaszewicz M., Trylska J., Taube M., Kozak M., Darżynkiewicz E., Grzela R.
    Academic press:
    International Journal of Molecular Sciences (rok: 2021, tom: 22, strony: 45679), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms222010929 - link to the publication
  6. N2 modified dinucleotide cap analogs as a potent tool for mRNA engineering.
    Authors:
    Grzela R., Piecyk K., Stankiewicz-Drogoń A., Pietrow P., Łukaszewicz M., Kurpiejewski K., Darżynkiewicz E., Jankowska-Anyszka M.
    Academic press:
    RNA (rok: 2023, tom: 29, strony: 200-216), Wydawca: Cold Spring Harbor Laboratory Press
    Status:
    Published
    DOI:
    10.1261/rna.079460.122 - link to the publication
  7. Development of bis-ANS-based modified fluorescence titration assay for IFIT/RNA studies
    Authors:
    Dobieżyńska A., Miszkiewicz J., Darżynkiewicz Z.M., Tyras M., Stankiewicz-Drogoń A., Trylska J., Darżynkiewicz E., Grzela R.
    Academic press:
    Biochemical and Biophysical Research Communications (rok: 2020, tom: 533, strony: 391-396), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bbrc.2020.09.006 - link to the publication
  8. N2 modifed cap analogues as translation inhibitors and substrates for preparation of therapeutic mRNA
    Authors:
    Karol Kurpiejewski, Marzena Jankowska‑Anyszka, Renata Grzela
    Academic press:
    European Biophysics Journal (rok: 2023, tom: 52, strony: 511–519), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s00249-023-01676-7 - link to the publication
  9. Insight into the Binding and Hydrolytic Preferences of hNudt16 Based on Nucleotide Diphosphate Substrates
    Authors:
    Chrabąszczewska M., Winiewska-Szajewska M., Ostrowska N., Bojarska E., Stępiński J., Mancewicz Ł., Łukaszewicz M., Trylska J., Taube M., Kozak M., Darżynkiewicz E., Grzela R.
    Academic press:
    International Journal of Molecular Sciences (rok: 2021, tom: 22, strony: 45679), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms222010929 - link to the publication

O Narodowym Centrum Nauki

Finansowanie nauki

Współpraca zagraniczna

Centrum prasowe

Deklaracja dostępności
Przetwarzanie danych osobowych
Polityka cookies

Partnerzy