Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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The effect of crowded environment on the catalytic activity and dynamics of viral proteases

2016/23/B/NZ1/03198

Keywords:

enzymatic reactions viral proteases macromolecular crowders cellular environment isothermal titration calorimetry fluorescence spectroscopy molecular dynamics simulations

Descriptors:

  • NZ1_2: Biochemistry
  • NZ1_3: Biophysics
  • NZ2_8: Computational biology

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

Uniwersytet Warszawski, Centrum Nowych Technologii Uniwersytetu Warszawskiego

woj. mazowieckie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Joanna Trylska 

Number of co-investigators in the project: 4

Call: OPUS 12 - announced on 2016-09-15

Amount awarded: 938 733 PLN

Project start date (Y-m-d): 2017-08-04

Project end date (Y-m-d): 2021-07-03

Project duration:: 47 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. Zestaw komputer z monitorem i podstawowym oprogramowaniem (2 szt.) (8 000 PLN)
  2. komputer przenośny (laptop).
  3. Homogenizator laboratoryjny (3 000 PLN)
  4. Miniwytrząsarka (1 845 PLN)
  5. Sonifikator (31 000 PLN)
  6. komputer plus monitor plus licencja.
  7. Miniwirówka (615 PLN)
  8. Oprogramowanie specjalistyczne (GraphPad Prism) (3 500 PLN)
  9. transiluminator UV (5 707 PLN)

Information in the final report

  • Publication in academic press/journals (10)
  • Book publications / chapters in book publications (1)
  1. Peptidomimetic inhibitors targeting the membrane-binding site of the T neutrophil proteinase 3
    Authors:
    Ksenia Maximova, Nathalie Reuter, Joanna Trylska
    Academic press:
    Biochimica et Biophysica Acta - Biomembranes (rok: 2019, tom: 1861, strony: 1502-1509), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bbamem.2019.06.009 - link to the publication
  2. Crowding affects structural dynamics and contributes to membrane association of the NS3/4A complex
    Authors:
    Natalia Ostrowska, Michael Feig, Joanna Trylska
    Academic press:
    Biophysical Journal (rok: 2021, tom: 120, strony: 45312), Wydawca: Cell Press
    Status:
    Published
    DOI:
    10.1016/j.bpj.2021.07.008 - link to the publication
  3. Enzyme kinetics in crowded solutions from isothermal titration calorimetry
    Authors:
    Ksenia Maximova, Jakub Wojtczak, Joanna Trylska
    Academic press:
    Analytical Biochemistry (rok: 2019, tom: 567, strony: 96-105), Wydawca: Elsevier Inc.
    Status:
    Published
    DOI:
    10.1016/j.ab.2018.11.006 - link to the publication
  4. Conformational Changes of Anoplin, W-MreB1–9, and (KFF)3K Peptides near the Membranes
    Authors:
    Monika Wojciechowska, Joanna Miszkiewicz, Joanna Trylska
    Academic press:
    Int. J. Mol. Sci. (rok: 2020, tom: 21, strony: 9672), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms21249672 - link to the publication
  5. Antibacterial Peptide Nucleic Acids—Facts and Perspectives
    Authors:
    Monika Wojciechowska, Marcin Równicki, Adam Mieczkowski, Joanna Miszkiewicz, Joanna Trylska
    Academic press:
    Molecules (rok: 2020, tom: 25(3), strony: 559), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/molecules25030559 - link to the publication
  6. Crowded environment affects the activity and inhibition of the NS3/4A protease
    Authors:
    Agnieszka Popielec, Natalia Ostrowska, Monika Wojciechowska, Michael Feig, Joanna Trylska
    Academic press:
    Biochimie (rok: 2020, tom: 176, strony: 169-180), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.biochi.2020.07.009 - link to the publication
  7. Enzymatic activity of human immunodeficiency virus type 1 protease in crowded solutions
    Authors:
    Ksenia Maximova, Jakub Wojtczak, Joanna Trylska
    Academic press:
    European Biophysics Journal (rok: 2019, tom: 48, strony: 685-689), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s00249-019-01392-1 - link to the publication
  8. Modeling Crowded Environment in Molecular Simulations
    Authors:
    Natalia Ostrowska, Michael Feig, Joanna Trylska
    Academic press:
    Frontiers in Molecular Biosciences (rok: 2019, tom: 6, strony: 86), Wydawca: Frontiers
    Status:
    Published
    DOI:
    10.3389/fmolb.2019.00086 - link to the publication
  9. Targeting type II toxin-antitoxin systems as antibacterial strategies
    Authors:
    Marcin Równicki, Robert Lasek, Joanna Trylska, Dariusz Bartosik
    Academic press:
    Toxins (rok: 2020, tom: 12, strony: 568), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/toxins12090568 - link to the publication
  10. Thermodynamics of the pseudo-knot in helix 18 of 16S ribosomal RNA
    Authors:
    Monika Wojciechowska, Marta Dudek, Joanna Trylska
    Academic press:
    Biopolymers (rok: 2018, tom: 109, strony: e23116), Wydawca: Wiley Periodicals Inc.
    Status:
    Published
    DOI:
    10.1002/bip.23116 - link to the publication
  1. Modeling Nucleic Acids at the Residue–Level Resolution
    Authors:
    Filip Leonarski, Joanna Trylska
    Book:
    Computational Methods to Study the Structure and Dynamics of Biomolecules and Biomolecular Processes (rok: 2019, tom: 8, strony: 117-161), Wydawca: Springer
    Status:
    Published

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