Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Application of new type of adsorbents and liquid chromatography hyphenated with mass spectrometry for the in vitro metabolism studies of antisense oligonucleotides for the antisense therapy purposes

2016/22/E/ST4/00478

Keywords:

antisense oligonucleotides metabolism liquid chromatography sample preparation mass spectrometry

Descriptors:

  • ST4_3: Molecular architecture and structure
  • ST4_6: Instrumental methods in chemistry
  • ST4_8: Organic chemistry

Panel:

ST4 - Chemistry: physical chemistry/chemical physics, theoretical chemistry, analytical chemistry, inorganic chemistry, organic chemistry, method development

Host institution :

Uniwersytet Mikołaja Kopernika, Wydział Chemii

woj. kujawsko-pomorskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr hab. Sylwia Studzińska 

Number of co-investigators in the project: 4

Call: SONATA BIS 6 - announced on 2016-06-15

Amount awarded: 1 321 149 PLN

Project start date (Y-m-d): 2017-06-02

Project end date (Y-m-d): 2020-12-01

Project duration:: 42 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. ultra wysokosprawny chromatograf cieczowy (300 000 PLN)

Information in the final report

  • Publication in academic press/journals (21)
  • Book publications / chapters in book publications (1)
  1. Analysis of Antisense Oligonucleotides and Their Metabolites with the Use of Ion Pair ReversedPhase Liquid Chromatography Coupled with Mass Spectrometry
    Authors:
    Anna Kaczmarkiewicz, Łukasz Nuckowski, Sylwia Studzińska, Bogusław Buszewski
    Academic press:
    Critical Review in Analytical Chemistry (rok: 2019, tom: 49, strony: 256-270), Wydawca: Taylor and Francis
    Status:
    Published
    DOI:
    10.1080/10408347.2018.1517034 - link to the publication
  2. Application of ion pair chromatography coupled with mass spectrometry to assess antisense oligonucleotide concentrations in living cells
    Authors:
    Sylwia Studzińska, Krzysztof Sobczak, Piotr Cywoniuk
    Academic press:
    Analyst (rok: 2019, tom: 144, strony: 622-633), Wydawca: Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/c8an01603h - link to the publication
  3. Poly(ionic liquid)s as new adsorbents in dispersive micro-solid phase extraction of unmodified and modified oligonucleotides
    Authors:
    Łukasz Nuckowski, Ewa Zalesińska, Krzysztof Dzieszkowski, Zbigniew Rafiński, Sylwia Studzińska
    Academic press:
    Talanta (rok: 2021, tom: 221, strony: 121662), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.talanta.2020.121662 - link to the publication
  4. Studying in vitro metabolism of the first and second generation of antisense oligonucleotides with the use of ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry
    Authors:
    Anna Kilanowska, Łukasz Nuckowski, Sylwia Studzińska,
    Academic press:
    Analytical and Bioanalytical Chemistry (rok: 2020, tom: 412, strony: 7453–7467), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s00216-020-02878-0 - link to the publication
  5. Analysis of microRNA and modified oligonucleotides with the use of ultra high performance liquid chromatography coupled with mass spectrometry
    Authors:
    Sylwia Studzińska, Bogusław Buszewski
    Academic press:
    Journal of Chromatography A (rok: 2018, tom: 1554, strony: 71-80), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.chroma.2018.04.037 - link to the publication
  6. Analysis of the first and second generation of antisense oligonucleotides in serum samples with the use of ultra high performance liquid chromatography coupled with tandem mass spectrometry
    Authors:
    Anna Kaczmarkiewicz, Łukasz Nuckowski, Sylwia Studzińska
    Academic press:
    Talanta (rok: 2019, tom: 196, strony: 54-63), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.talanta.2018.12.023 - link to the publication
  7. Hydrophilic interaction in solid phase extraction of antisense oligonucleotides
    Authors:
    Łukasz Nuckowski, Anna Kilanowska, Sylwia Studzińska
    Academic press:
    Journal of Chromatographic Science (rok: 2020, tom: 58, strony: 383–387), Wydawca: Oxford Academic
    Status:
    Published
    DOI:
    10.1093/chromsci/bmz114 - link to the publication
  8. In vivo and in vitro studies of antisense oligonucleotides - a review
    Authors:
    Anna Kilanowska, Sylwia Studzińska
    Academic press:
    RSC Advances (rok: 2020, tom: 10, strony: 34501-34516), Wydawca: Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/d0ra04978f - link to the publication
  9. Synthesis and application of stationary phase for DNA affinity chromatographic analysis of unmodified and antisense oligonucleotide
    Authors:
    Sylwia Studzińska, Ewelina Zawadzka, Szymon Bocian, Michał Szumski
    Academic press:
    Analytical and Bioanalytical Chemistry (rok: 2021, tom: 413, strony: 5109–5119), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s00216-021-03473-7 - link to the publication
  10. Analiza oligonukleotydów antysensownych pierwszej i drugiej generacji za pomocą chromatografii par jonowych
    Authors:
    Anna Kaczmarkiewicz, Łukasz Nuckowski, Sylwia Studzińska, Bogusław Buszewski
    Academic press:
    Analityka (rok: 2019, tom: 2, strony: 45393), Wydawca: Malamut
    Status:
    Published
    DOI:
    brak - link to the publication
  11. Analysis of antisense oligonucleotides with the use of ionic liquids as the mobile phase modifiers
    Authors:
    Anna Kaczmarkiewicz, Judyta Zielak, Łukasz Nuckowski, Sylwia Studzińska
    Academic press:
    RSC Advances (rok: 2019, tom: 9, strony: 39100-39110), Wydawca: Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/c9ra06483d - link to the publication
  12. Application of Magnetic Nanoparticles Coated with Crosslinked Zwitterionic Poly(ionic liquid)s for the Extraction of Oligonucleotides
    Authors:
    Łukasz Nuckowski, Krzysztof Dzieszkowski, Zbigniew Rafiński, Sylwia Studzińska
    Academic press:
    Materials (rok: 2021, tom: 14 (12), strony: 3146), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ma14123146 - link to the publication
  13. Klasyczne i nowe metody przygotowania próbek oligonukleotydów antysensownych przed analizą chromatograficzną
    Authors:
    Łukasz Nuckowski, Anna Kaczmarkiewicz, Sylwia Studzińska
    Academic press:
    Analityka (rok: 2020, tom: 1, strony: 45393), Wydawca: Malamut
    Status:
    Published
    DOI:
    brak - link to the publication
  14. Zastosowanie cieczy jonowych jako modyfikatorów fazy ruchomej w chromatografii cieczowej
    Authors:
    Anna Kilanowska, Judyta Zielak, Sylwia Studzińska
    Academic press:
    Wiadomości Chemiczne (rok: 2020, tom: 74 (7-8), strony: 507-525), Wydawca: Polskie Towarzystwo Chemiczne
    Status:
    Published
    DOI:
    brak - link to the publication
  15. Adsorbenty ze związanymi cieczami jonowymi i ich wykorzystanie w przygotowaniu próbek oligonukleotydów
    Authors:
    Łukasz Nuckowski, Ewa Zalesińska, Sylwia Studzińska
    Academic press:
    Wiadomości Chemiczne (rok: 2020, tom: 74 (7-8), strony: 545-565), Wydawca: Polskie Towarzystwo Chemiczne
    Status:
    Published
    DOI:
    brak - link to the publication
  16. Application of hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry for the retention and sensitivity studies of antisense oligonucleotides
    Authors:
    Anna Kilanowska, Bogusław Buszewski, Sylwia Studzińska
    Academic press:
    Journal of Chromatography A (rok: 2020, tom: 1622, strony: 461100), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.chroma.2020.461100 - link to the publication
  17. Attachment of hybridizable oligonucleotide to silica support and its application for selective extraction of unmodified and antisense oligonucleotides from serum samples
    Authors:
    Sylwia Studzińska, Magdalena Skoczylas, Szymon Bocian, Anna Dembska, Bogusław Buszewski,
    Academic press:
    RSC Advances (rok: 2020, tom: 10, strony: 16221-16230), Wydawca: Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/d0ra01620a - link to the publication
  18. Ultra high performance reversed phase liquid chromatography hyphenated with ESI-Q-TOF-MS for the analysis of unmodified and antisense oligonucleotides
    Authors:
    Sylwia Studzińska, Łukasz Nuckowski, Anna Kilanowska
    Academic press:
    Chromatographia (rok: 2020, tom: 83, strony: 349–360), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s10337-019-03844-4 - link to the publication
  19. A new approach to preparation of antisense oligonucleotide samples with microextraction by packed sorbent
    Authors:
    Łukasz Nuckowski, Anna Kaczmarkiewicz, Sylwia Studzińska, Bogusław Buszewski
    Academic press:
    Analyst (rok: 2019, tom: 144, strony: 4622-4632), Wydawca: Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/c9an00740g - link to the publication
  20. Development of SPE method for the extraction of phosphorothioate oligonucleotides from serum samples
    Authors:
    Łukasz Nuckowski, Anna Kaczmarkiewicz, Sylwia Studzińska
    Academic press:
    Bioanalysis (rok: 2018, tom: 10 (20), strony: 1757-6180), Wydawca: Future Science
    Status:
    Published
    DOI:
    10.4155/bio-2018-0166 - link to the publication
  21. Review on sample preparation methods for oligonucleotides analysis by liquid chromatography
    Authors:
    Łukasz Nuckowski, Anna Kaczmarkiewicz, Sylwia Studzińska
    Academic press:
    Journal of Chromatography B (rok: 2018, tom: 1090, strony: 90-100), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.jchromb.2018.05.025 - link to the publication
  1. Analityka oligonukleotydów antysensownych
    Authors:
    Sylwia Studzińska, Anna Kilanowska, Łukasz Nuckowski, Bogusław Buszewski
    Book:
    Bioanalityka w nauce i życiu (rok: 2020, tom: 1, strony: 57-71), Wydawca: PWN
    Status:
    Published

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