Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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New method for determination of equilibrium constant for complex formation, concentrations and diffusion coefficients in solutions and living cells at nanomolar down to picomolar concentration of reagents.

2016/22/A/ST4/00017

Keywords:

diffusion limited reactions equilibrium constant fluorescence correlation spectroscopy

Descriptors:

  • ST4_7: Electrochemistry, microfluidics in chemistry, sensors
  • ST4_13: Solid-state chemistry, surface physicochemistry
  • ST3_15: Statistical physics of condensed matter, phase transitions, noise and fluctuations, etc.

Panel:

ST4 - Chemistry: physical chemistry/chemical physics, theoretical chemistry, analytical chemistry, inorganic chemistry, organic chemistry, method development

Host institution :

Instytut Chemii Fizycznej Polskiej Akademii Nauk

woj. mazowieckie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Robert Hołyst 

Number of co-investigators in the project: 8

Call: MAESTRO 8 - announced on 2016-06-15

Amount awarded: 2 382 800 PLN

Project start date (Y-m-d): 2017-04-13

Project end date (Y-m-d): 2021-10-12

Project duration:: 54 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. laserowa linia 560 nm (150 000 PLN)
  2. upgrade systemu Picoquanta (diodowy laser pulsowy, sterowniki do wielu laserów, ukłąd laserowy filtry, zwierciadła) (210 000 PLN)

Information in the final report

  • Publication in academic press/journals (25)
  1. Fast and efficient deposition of broad range of analytes on substrates for surface enhanced Raman spectroscopy
    Authors:
    Richter, L ; Albrycht, P; Ksiezopolska-Gocalska, M; Pobozy, E ; Bachlinski, R ; Sashuk, V ; Paczesny, J ; Holyst, R
    Academic press:
    BIOSENSORS & BIOELECTRONICS (rok: 2020, tom: 156, strony: 112124), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.bios.2020.112124 - link to the publication
  2. Ions in an AC Electric Field: Strong Long-Range Repulsion between Oppositely Charged Surfaces
    Authors:
    Richter, L ; Zuk, PJ ; Szymczak, P ; Paczesny, J ; Bak, KM ; Szymborski, T ; Garstecki, P ; Stone, HA ; Holyst, R ; Drummond, C
    Academic press:
    Physical Review Letters (rok: 2020, tom: 125, strony: 56001), Wydawca: APS
    Status:
    Published
    DOI:
    10.1103/PhysRevLett.125.056001 - link to the publication
  3. Kinetics and equilibrium constants of oligonucleotides at low concentrations. Hybridization and melting study
    Authors:
    Bielec, K ; Sozanski, K ; Seynen, M ; Dziekan, Z ; ten Wolde, PR; Holyst, R
    Academic press:
    PHYSICAL CHEMISTRY CHEMICAL PHYSICS (rok: 2019, tom: 21, strony: 10798-10807), Wydawca: RSC
    Status:
    Published
    DOI:
    10.1039/c9cp01295h - link to the publication
  4. Quantifying Nanoscale Viscosity and Structures of Living Cells Nucleus from Mobility Measurements
    Authors:
    Grzegorz Bubak, Karina Kwapiszewska, Tomasz Kalwarczyk, Krzysztof Bielec, Tomasz Andryszewski, Michalina Iwan, Szymon Bubak, and Robert Hołyst
    Academic press:
    Journal of Physical Chemistry Letters (rok: 2021, tom: 12, strony: 294-301), Wydawca: ACS
    Status:
    Published
    DOI:
    10.1021/acs.jpclett.0c03052 - link to the publication
  5. Recent advances in bacteriophage-based methods for bacteria detection
    Authors:
    Richter, L; Janczuk-Richter ; Niedziolka-Jonsson, J ; Paczesny, J ; Holyst, R
    Academic press:
    Drug Discovery Today (rok: 2018, tom: 23, strony: 448-455), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.drudis.2017.11.007 - link to the publication
  6. Scaling equation for viscosity of polydimethylsiloxane in ethyl acetate: From dilute to concentrated solutions
    Authors:
    Agasty, A; Wisniewska, A ; Kalwarczyk, T ; Koynov, K ; Holyst, R
    Academic press:
    Polymer (rok: 2020, tom: 203, strony: 122779), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.polymer.2020.122779 - link to the publication
  7. Analysis of Brightness of a Single Fluorophore for Quantitative Characterization of Biochemical Reactions
    Authors:
    Bielec, K ; Bubak, G ; Kalwarczyk, T ; Holyst, R
    Academic press:
    Journal of Physical Chemistry B (rok: 2020, tom: 124, strony: 1941-1948), Wydawca: ACS
    Status:
    Published
    DOI:
    10.1021/acs.jpcb.0c00770 - link to the publication
  8. Cell extract gels as an example of active matter
    Authors:
    Wisniewska, A ; Kalwarczyk, T ; Szymanski, J ; Kryszczuk, K ; Matula, K ; Holyst, R
    Academic press:
    Rheologica Acta (rok: 2020, tom: 59, strony: 575-582), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s00397-020-01213-9 - link to the publication
  9. Joint effect of surfactants and cephalexin on the formation of Escherichia coli filament
    Authors:
    Hou, S ; Jia, ZZ ; Kryszczuk, K ; Chen, D ; Wang, LN ; Holyst, R ; Feng, XZ
    Academic press:
    ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (rok: 2020, tom: 199, strony: 110750), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.ecoenv.2020.110750 - link to the publication
  10. Propagation of Oscillating Chemical Signals through Reaction Networks
    Authors:
    Roszak, R; Bajczyk, MD ; Gajewska, EP; Holyst, R; Grzybowski, BA
    Academic press:
    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (rok: 2019, tom: 58, strony: 4520-4525), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/anie.201808821 - link to the publication
  11. Nanoscale Viscosity of Cytoplasm Is Conserved in Human Cell Lines
    Authors:
    Kwapiszewska, K ; Szczepanski, K ; Kalwarczyk, T ; Michalska, B ; Patalas-Krawczyk, P; Szymanski, J ; Andryszewski, T ; Iwan, M ; Duszynski, J ; Holyst, R
    Academic press:
    JOURNAL OF PHYSICAL CHEMISTRY LETTERS (rok: 2020, tom: 11, strony: 6914-6920), Wydawca: ACS
    Status:
    Published
    DOI:
    10.1021/acs.jpclett.0c01748 - link to the publication
  12. Nanoscopic Approach to Quantification of Equilibrium and Rate Constants of Complex Formation at Single-Molecule Level
    Authors:
    Zhang, XZ ; Sisamakis, E ; Sozanski, K ; Holyst, R
    Academic press:
    JOURNAL OF PHYSICAL CHEMISTRY LETTERS (rok: 2017, tom: 8, strony: 5785-5791), Wydawca: ACS
    Status:
    Published
    DOI:
    10.1021/acs.jpclett.7b02742 - link to the publication
  13. Flux and storage of energy in nonequilibrium stationary states
    Authors:
    Holyst, R ; Maciolek, A ; Zhang, YR ; Litniewski, M ; Knychala, P ; Kasprzak, M ; Banaszak, M
    Academic press:
    PHYSICAL REVIEW E (rok: 2019, tom: 99, strony: 42118), Wydawca: APS
    Status:
    Published
    DOI:
    10.1103/PhysRevE.99.042118 - link to the publication
  14. Photoluminescent, Ferromagnetic, and Hydrophobic Sponges for Oil-Water Separation
    Authors:
    Yin, KY ; Lu, DD ; Sun, BP ; Kalwarczyk, T ; Holyst, R ; Hao, JY ; Li, HG ; Hao, JC
    Academic press:
    ACS Omega (rok: 2020, tom: 5, strony: 15077-15082), Wydawca: ACS
    Status:
    Published
    DOI:
    10.1021/acsomega.0c00899 - link to the publication
  15. Stability of cytoplasmic nanoviscosity during cell cycle of HeLa cells synchronized with Aphidicolin
    Authors:
    Szczepanski, K; Kwapiszewska, K ; Holyst, R
    Academic press:
    SCIENTIFIC REPORTS (rok: 2019, tom: 9, strony: 16486), Wydawca: Nature group
    Status:
    Published
    DOI:
    10.1038/s41598-019-52758-6 - link to the publication
  16. Quantitative fluorescence correlation spectroscopy in three-dimensional systems under stimulated emission depletion conditions
    Authors:
    Sozanski, K ; Sisamakis, E ; Zhang, XZ ; Holyst, R
    Academic press:
    OPTICA (rok: 2017, tom: 4, strony: 982-988), Wydawca: OSA
    Status:
    Published
    DOI:
    10.1364/OPTICA.4.000982 - link to the publication
  17. Transport of nanoprobes in multicellular spheroids
    Authors:
    Pilz, M ; Kwapiszewska, K ; Kalwarczyk, T ; Bubak, G ; Nowis, D ; Holyst, R
    Academic press:
    Nanoscale (rok: 2020, tom: 12 ‏, strony: 19880-19887), Wydawca: Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/d0nr01986k - link to the publication
  18. Diffusion and flow in complex liquids
    Authors:
    Makuch, K ; Holyst, R; Kalwarczyk, T ; Garstecki, P; Brady, JF
    Academic press:
    SOFT MATTER (rok: 2020, tom: 16, strony: 114-124), Wydawca: RSC
    Status:
    Published
    DOI:
    10.1039/c9sm01119f - link to the publication
  19. Influence of molecular rebinding on the reaction rate of complex formation
    Authors:
    Tomasz Kalwarczyk, Krzysztof Bielec, Krzysztof Burdzy, Robert Holyst
    Academic press:
    Physical Chemistry Chemical Physics (rok: 2021, tom: 23, strony: 19343-19351), Wydawca: Royal Society of Chemistry (RSC)
    Status:
    Published
    DOI:
    10.1039/d1cp02820k - link to the publication
  20. Macroscopic Viscosity of Polymer Solutions from the Nanoscale Analysis
    Authors:
    Agasty, Airit, Wisniewska, Agnieszka, Kalwarczyk, Tomasz, Koynov, Kaloian, Holyst, Robert
    Academic press:
    ACS APPLIED POLYMER MATERIALS (rok: 2021, tom: 3, strony: 2813-2822), Wydawca: ACS
    Status:
    Published
    DOI:
    10.1021/acsapm.1c00348 - link to the publication
  21. Phenotypic plasticity of Escherichia coli upon exposure to physical stress induced by ZnO nanorods
    Authors:
    Matula, K ; Richter, L; Janczuk-Richter, M ; Nogala, W; Grzeszkowiak, M ; Peplinska, B; Jurga, S; Wyroba, E ; Suski, S; Bilski, H ; Silesian, A ; Bluyssen, HAR ; Derebecka, N ; Wesoly, J ; Los, JM ; Los, M ; Decewicz, P ; Dziewit, L ; Paczesny, J ; Holyst, R
    Academic press:
    SCIENTIFIC REPORTS (rok: 2019, tom: 9, strony: 8575), Wydawca: Nature group
    Status:
    Published
    DOI:
    10.1038/s41598-019-44727-w - link to the publication
  22. Storage of Energy in Constrained Non-Equilibrium Systems
    Authors:
    Zhang, YR ; Gizynski, K ; Maciolek, A ; Holyst, R
    Academic press:
    Entropy (rok: 2020, tom: 22, strony: 557), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/e22050557 - link to the publication
  23. Fluorescence correlation spectroscopy for multiple-site equilibrium binding: a case of doxorubicin-DNA interaction
    Authors:
    Zhang, XZ ; Poniewierski, A; Sozanski, K ; Zhou, Y ; Brzozowska-Elliott, A ; Holyst, R
    Academic press:
    PHYSICAL CHEMISTRY CHEMICAL PHYSICS (rok: 2019, tom: 21, strony: 1572-1577), Wydawca: RSC
    Status:
    Published
    DOI:
    10.1039/c8cp06752j - link to the publication
  24. Self-Stabilized Giant Aggregates in Water from Room-Temperature Ionic Liquids with an Asymmetric Polar-Apolar-Polar Architecture
    Authors:
    Zhang, GP ; Zhu, HX ; Chen, JF ; Chen, MJ ; Kalwarczyk, T; Holyst, R ; Li, HG ; Hao, JC
    Academic press:
    Journal of Physical Chemistry B (rok: 2020, tom: 124, strony: 4651-4660), Wydawca: ACS
    Status:
    Published
    DOI:
    10.1021/acs.jpcb.0c02283 - link to the publication
  25. Single-molecule brightness analysis for the determination of anticancer drug interactions with DNA
    Authors:
    Zhou, Y ; Bielec, K; Pasitsuparoad, P ; Holyst, R
    Academic press:
    Analyst (rok: 2020, tom: ‏ 145, strony: ‏ 6600-6606), Wydawca: Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/d0an01108h - link to the publication

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