Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Chemically modified mRNA for studies on cellular processes and therapeutic applications

2016/21/B/ST5/02556

Keywords:

nucleotide synthesis nucleotide analogs massanger RNA anti-cancer therapy mRNA degradation

Descriptors:

  • ST5_16:
  • ST5_11: Smart materials – self assembled materials, external stimuli-responsive materials
  • NZ1_2: Biochemistry

Panel:

ST5 - Materials: materials synthesis, structure-properties relations, advanced and functional materials with designed properties, (macro)molecular architecture, material engineering

Host institution :

Uniwersytet Warszawski, Centrum Nowych Technologii Uniwersytetu Warszawskiego

woj. mazowieckie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Jacek Jemielity 

Number of co-investigators in the project: 8

Call: OPUS 11 - announced on 2016-03-15

Amount awarded: 1 651 800 PLN

Project start date (Y-m-d): 2017-02-23

Project end date (Y-m-d): 2020-10-22

Project duration:: 44 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. Termoforeza mikroskalowa (500 000 PLN)

Information in the final report

  • Publication in academic press/journals (9)
  • Articles in post-conference publications (2)
  • Book publications / chapters in book publications (1)
  1. Azido-Functionalized 5′ Cap Analogues for the Preparation of Translationally Active mRNAs Suitable for Fluorescent Labeling in Living Cells
    Authors:
    Adam Mamot, Pawel J. Sikorski, Marcin Warminski, Joanna Kowalska, Jacek Jemielity
    Academic press:
    Angewandte Chemie International Edition (rok: 2017, tom: 56, strony: 15628 –15632), Wydawca: Wiley-VCH Verlag GmbH &Co. KGaA, Weinheim
    Status:
    Published
    DOI:
    10.1002/anie.201709052 - link to the publication
  2. Phosphodiester modifications in mRNA polyA tail prevent deadenylation without compromising protein expression
    Authors:
    Dominika Strzelecka, Miroslaw Smietanski, Pawel J. Sikorski, Marcin Warminski, Joanna Kowalska, Jacek Jemielity
    Academic press:
    RNA (rok: 2020, tom: 26 (12), strony: 1815-1837), Wydawca: Cold Spring Harbor Laboratory Press for the RNA Society
    Status:
    Published
    DOI:
    10.1261/rna.077099.120 - link to the publication
  3. Synthesis of RNA 5′-Azides from 2′-O-Pivaloyloxymethyl-Protected RNAs and Their Reactivity in Azide–Alkyne Cycloaddition Reactions
    Authors:
    Marcin Warmiński, Joanna Kowalska, Jacek Jemielity
    Academic press:
    Organic Letters (rok: 2017, tom: 19, strony: 3624-3627), Wydawca: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/acs.orglett.7b01591 - link to the publication
  4. mRNAs biotinylated within the 5′ cap and protected against decapping: new tools to capture RNA–protein complexes
    Authors:
    Sylwia Bednarek , Vanesa Madan , Pawel J. Sikorski , Ralf Bartenschlager , Joanna Kowalska , and Jacek Jemielity
    Academic press:
    Philosophical Transactions of The Royal Society B (rok: 2018, tom: 373, strony: 20180167), Wydawca: The Royal Society
    Status:
    Published
    DOI:
    10.1098/rstb.2018.0167 - link to the publication
  5. Structural Insights into the Interaction of Clinically Relevant Phosphorothioate mRNA Cap Analogs with Translation Initiation Factor 4E Reveal Stabilization via Electrostatic Thio-Effect
    Authors:
    Marcin Warminski, Joanna Kowalska, Elzbieta Nowak, Dorota Kubacka, Ryan Tibble, Renata Kasprzyk, Pawel J. Sikorski, John D. Gross, Marcin Nowotny, Jacek Jemielity
    Academic press:
    ACS Chemical Biology (rok: 2021, tom: 16, strony: ‏ 334-343), Wydawca: ACS
    Status:
    Published
    DOI:
    10.1021/acschembio.0c00864 - link to the publication
  6. The identity and methylation status of the first transcribed nucleotide in eukaryotic mRNA 5′ cap modulates protein expression in living cells
    Authors:
    Paweł J. Sikorski, Marcin Warmiński, Dorota Kubacka, Tomasz Ratajczak, Dominika Nowis, Jonanna Kowalska, Jacek Jemielity
    Academic press:
    Nucleic Acids Research (rok: 2020, tom: 48, strony: 1607-1626), Wydawca: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/nar/gkaa032 - link to the publication
  7. mRNA cap analogues substituted in the tetraphosphate chain with CX2: identification of O-to-CCl2 as the first bridging modification that confers resistance to decapping without impairing translation
    Authors:
    Anna M. Rydzik Marcin Warminski Pawel J. Sikorski Marek R. Baranowski Sylwia Walczak Joanna Kowalska Joanna Zuberek Maciej Lukaszewicz Elzbieta Nowak Timothy D. W. Claridge Edward Darzynkiewicz Marcin Nowotny Jacek Jemielity
    Academic press:
    Nucleic Acids Research (rok: 2017, tom: 45, strony: 8661-8675), Wydawca: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/nar/gkx569 - link to the publication
  8. 5′-fluoro(di)phosphate-labeled oligonucleotides are versatile molecular probes for studying nucleic acid secondary structure and interactions by 19F NMR
    Authors:
    Marek R Baranowski, Marcin Warminski, Jacek Jemielity, Joanna Kowalska
    Academic press:
    Nucleic Acids Research (rok: 2020, tom: 48 (15), strony: 8209-8224), Wydawca: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/nar/gkaa470 - link to the publication
  9. Exploring the potential of phosphotriazole 5' mRNA cap analogues as efficient translation initiators
    Authors:
    Sylwia Walczak, Paweł J. Sikorski, Renata Kasprzyk, Joanna Kowalska, Jacek Jemielity
    Academic press:
    Organic & Biomolecular Chemistry (rok: 2018, tom: 16, strony: 6741-6748), Wydawca: The Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/c8ob01720d - link to the publication
  1. Site specific, sequence independent fluorescent labeling of mRNA in living cells
    Authors:
    Jacek Jemielity, Adam Mamot, Pawel J. Sikorski, Marcin Warminski, Joanna Kowalska
    Conference:
    XXIII International Roundtable On Nucleosides, Nucleotides And Nucleic Acids (rok: 2018, ), Wydawca: University of California
    Data:
    konferencja 26-30.08.2018
    Status:
    Published
  2. Kolorymetryczna sonda molekularna do monitorowania aktywności eIF4E - białka wiążącego strukturę końca 5' mRNA
    Authors:
    Perzanowska O., Majewski M., Kowalska J., Jemielity J.
    Conference:
    62 Zjazd Naukowy Polskiego Towarzystwa Chemicznego (rok: 2019, ), Wydawca: Polskie Towarzystwo Chemiczne
    Data:
    konferencja 2-6.09.2019
    Status:
    Published
  1. Solid-Phase Synthesis of RNA 5 -Azides and Their Application for Labeling, Ligation, and Cyclization Via Click Chemistry
    Authors:
    Marcin Warminski, Joanna Kowalska, Jacek Jemielity
    Book:
    Current Protocols in Nucleic Acid Chemistry (rok: 2020, tom: 82, strony: brak), Wydawca: Wiley Periodicals LLC
    Status:
    Published

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