Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Structural studies of cold-adapted enzymes - mechanism of cold adaptation

2016/21/B/ST5/00555

Keywords:

crystal structure enzyme cold-adapted hydrolases transferases reaction mechanism

Descriptors:

  • ST5_16:

Panel:

ST5 - Materials: materials synthesis, structure-properties relations, advanced and functional materials with designed properties, (macro)molecular architecture, material engineering

Host institution :

Politechnika Łódzka, Wydział Biotechnologii i Nauk o Żywności

woj. łódzkie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr hab. Anna Bujacz 

Number of co-investigators in the project: 7

Call: OPUS 11 - announced on 2016-03-15

Amount awarded: 871 900 PLN

Project start date (Y-m-d): 2017-01-18

Project end date (Y-m-d): 2020-01-17

Project duration:: 36 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. Szafa chłodnicza dwudrzwiowa ze szklanymi drzwiami (16 000 PLN)
  2. Zestaw komputerowy ze stacją do grafiki 3D (20 000 PLN)
  3. FPLC system chromatograficzny (240 000 PLN)
  4. Termocykler PCR z zestawem do elektroforezy poziomej (20 000 PLN)

Information in the final report

  • Publication in academic press/journals (6)
  1. Active Site Architecture and Reaction Mechanism Determination of Cold Adapted β-d-galactosidase from Arthrobacter sp. 32cB
    Authors:
    Rutkiewicz M, Bujacz A*, Wanarska M, Wierzbicka-Wos A, Cieslinski H
    Academic press:
    International Journal of Molecular Sciences (rok: 2019, tom: 20/4301, strony: 45339), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms20174301 - link to the publication
  2. In Situ Random Microseeding and Streak Seeding Used for Growth of Crystals of Cold-Adapted β-DGalactosidases: Crystal Structure of βDG from Arthrobacter sp. 32cB
    Authors:
    Rutkiewicz-Krotewicz M., Pietrzyk-Brzezinska A.J., Wanarska M., Cieslinski H., Bujacz A.
    Academic press:
    Crystals (rok: 2018, tom: 8, strony: 13-28), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/cryst8010013 - link to the publication
  3. Structural features of cold-adapted dimeric GH2 β-D-galactosidase from Arthrobacter sp. 32cB
    Authors:
    Rutkiewicz M., Bujacz A.*, Bujacz G.
    Academic press:
    BBA - Proteins and Proteomics (rok: 2019, tom: 1867, strony: 776-786), Wydawca: ELSEVIER
    Status:
    Published
    DOI:
    10.1016/j.bbapap.2019.06.001 - link to the publication
  4. Architecture of β-D-galactosidase active site as a basis for enzyme engineering
    Authors:
    Rutkiewicz-Krotewicz M., Bujacz A., Wanarska M., Cieslinski H.
    Academic press:
    Acta Crystallographica Section A (rok: 2017, tom: 73, strony: C278), Wydawca: IUCr
    Status:
    Published
    DOI:
    10.1107/S2053273317092956 - link to the publication
  5. Mapping the Transglycosylation Relevant Sites of Cold-Adapted β-D-Galactosidase from Arthrobacter sp. 32cB
    Authors:
    Maria Rutkiewicz, Marta Wanarska and Anna Bujacz
    Academic press:
    International Journal of Molecular Sciences (rok: 2020, tom: 21, strony: 45310), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms21155354 - link to the publication
  6. Structural evidence of active site adaptability towards different sized substrates of aromatic amino acid aminotransferase from Psychrobacter Sp. B6
    Authors:
    Anna Bujacz*, Jedrzej Rum, Maria Rutkiewicz, Agnieszka J. Pietrzyk-Brzezinska and Grzegorz Bujacz
    Academic press:
    Materials (rok: 2021, tom: 14, strony: 45310), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ma14123351 - link to the publication

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