Molecular basis of regulation of electron flow between membranous quinone and water-soluble cytochrome. Does mitochondrial complex III switch between "slow" and "fast" state?
2015/18/A/NZ1/00046
Keywords
Descriptors
- NZ1_3: Biofizyka
- NZ1_2: Biochemia
- NZ1_1: Biologia molekularna
Panel
NZ1 – Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology
Host institution
Uniwersytet Jagielloński, Wydział Biochemii, Biofizyki i Biotechnologii
Voivodeship: małopolskie
Principal investigator (from the host institution)
Number of co-investigators in the project: 6
Call
MAESTRO 7 – announced on 2015-06-15
- Amount awarded
- 2 499 555 PLN
- Project start date
- 2016-09-01
- Project end date
- 2022-08-31
- Project duration
- 72 months (the same as in the proposal)
- Project status
- Project settled
Project description
Download the project description in a pdf file
Note — project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.
Equipment purchased [PL]
- Mechaniczny homogenizator tkankowy. Cost: 3 000 PLN
- Monochromator ze światłowodami. Cost: 15 000 PLN
- Rezonator EPR Bruker ER4104OR. Cost: 96 000 PLN
- Spektrofotometr do mikroobjętości DS-11.
Information in the final report
Publication in academic press/journals (9)
-
Hydrogen bonding rearrangement by a mitochondrial disease mutation in cytochrome bc1 perturbs heme bH redox potential and spin state
- Authors:
- Patryk Kuleta, Jonathan Lasham, Marcin Sarewicz, Iwona Ekiert, Vivek Sharma, Robert Ekiert, Artur Osyczka
- Academic press:
- Proceedings of National Academy of Sciences USA (year: 2021, volume: 118, pages: e2026169118), Publisher: National Academy of Sciences
- Status:
- Published
- DOI:
- 10.1073/pnas.2026169118 – link to the publication
-
The high-spin heme bL mutant exposes dominant reaction leading to formation of the semiquinone spin coupled to [2Fe-2S]+ cluster at the Qo site of Rhodobacter capsulatus cytochrome bc1
- Authors:
- Marcin Sarewicz, Sebastian Pintscher, Łukasz Bujnowicz, Małgorzata Wolska, Artur Osyczka
- Academic press:
- Frontiers in Chemistry (year: 2021, volume: 9, pages: 658877), Publisher: Frontiers Media S.A.
- Status:
- Published
- DOI:
- 10.3389/fchem.2021.658877 – link to the publication
-
A spontaneous mitonuclear epistasis converging on Rieske Fe-S protein exacerbates complex III deficiency in mice
- Authors:
- J. Purhonen, V. Grigorjev, R. Ekiert, N. Aho, J. Rajendran, R. Pietras, K. Truve, M. Wkistrom, V. Sharma, A. Osyczka, V. Fellman, J. Kallijarvi
- Academic press:
- Nature Communications (year: 2020, volume: 11, pages: 322), Publisher: Springer Nature
- Status:
- Published
- DOI:
- 10.1038/s41467-019-14201-2 – link to the publication
-
Supression of superoxide production by a spin-spin coupling bewteen semiquinone and the Rieske cluster in cytochrome bc1
- Authors:
- Łukasz Bujnowicz, Arkadiusz Borek, Patryk Kuleta, Marcin Sarewicz, Artur Osyczka
- Academic press:
- Febs Letters (year: 2019, volume: 593, pages: 46093), Publisher: Wiley
- Status:
- Published
- DOI:
- 10.1002/1873-3468.13296 – link to the publication
-
Functional flexibility of electron flow between quinol oxidation Qo site of cytochrome bc1 and cytochrome c revealed by combinatory effects of mutations in cytochrome b, iron-sulfur protein and cytochrome c1
- Authors:
- Arkadiusz Borek, Robert Ekiert, Artur Osyczka
- Academic press:
- Biochimica et Biophysica Acta - Bioenergetics (year: 2018, volume: 1859, pages: 754-761), Publisher: Elsevier
- Status:
- Published
- DOI:
- 10.1016/j.bbabio.2018.04.010 – link to the publication
-
Generation of semiquinone-[2Fe-2S]+ spin-coupled center at the Qo site of cytochrome bc1 in redox-poised, illuminated photosynthetic membranes from Rhodobacter capsulatus
- Authors:
- Marcin Sarewicz, Łukasz Sarewicz, Artur Osyczka
- Academic press:
- Biochimica et Biophysica Acta - Bioenergetics (year: 2018, volume: 1859, pages: 145-153), Publisher: Elsevier
- Status:
- Published
- DOI:
- 10.1016/j.bbabio.2017.11.006 – link to the publication
-
Catalytic Reactions and Energy Conservation in the Cytochromebc1andb6fComplexes of Energy-Transducing Membranes
- Authors:
- Marcin Sarewicz, Sebastian Pintscher, Rafał Pietras, Arkadiusz Borek,Łukasz Bujnowicz, Guy Hanke,William A. Cramer, Giovanni Finazzi, Artur Osyczka
- Academic press:
- Chemical Reviews (year: 2021, volume: 121, pages: 2020–2108), Publisher: ACS
- Status:
- Published
- DOI:
- 10.1021/acs.chemrev.0c00712 – link to the publication
-
Charge polarization imposed by the binding site facilitates enzymatic redox reactions of quinone
- Authors:
- Sebastian Pintscher, Anna Wójcik-Augustyn, Marcin Sarewicz, Artur Osyczka
- Academic press:
- Biochimica et Biophysica Acta -Bioenergetics (year: 2020, volume: 1861, pages: 148216), Publisher: Elsevier
- Status:
- Published
- DOI:
- 10.1016/j.bbabio.2020.148216 – link to the publication
-
Electron sweep across four b-hemes of cytochrome bc1 revealed by unusual paramagnetic properties of the Qi semiquinone intermediate
- Authors:
- Sebastian Pintscher, Rafał Pietras, Marcin Sarewicz, Artur Osyczka
- Academic press:
- Biochimica et Biophysica Acta - Bioenergetics (year: 2018, volume: 1859, pages: 459-469), Publisher: Elsevier
- Status:
- Published
- DOI:
- 10.1016/j.bbabio.2018.03.010 – link to the publication
Book publications / chapters in book publications (2)
-
Cytochrome bc1 complex (Respiratory Chain Complex III)
- Authors:
- Marcin Sarewicz, Artur Osyczka
- Book:
- Encyclopedia of Biological Chemistry, 3rd Edition (year: 2021, volume: 2, pages: 502-511), Publisher: Elsevier
- Status:
- Published
-
Advances in understanding mechanism and physiology of cytochromes bc
- Authors:
- Arkadiusz Borek, Robert Ekiert, Artur Osyczka
- Book:
- Mechanisms of primary energy transduction in biology (year: 2018, volume: 5, pages: 192-214), Publisher: The Royal Society of Chemistry
- Status:
- Published