Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Synthesis and evaluation of a novel toolkit for biology and biotechnology based on adenine nucleotides analogs

2015/18/E/ST5/00555

Keywords:

nucleotides chemical synthesis sulfotransferase pyrophosphatase kinase becterial cap nucleotide analogs phosphate modifications PAPS ATP NAD sulfurylation phosphorylation enzymatic hydrolysis bioorganic chemistry chemical biology

Descriptors:

  • ST5_16:
  • ST5_23:
  • NZ1_2: Biochemistry

Panel:

ST5 - Materials: materials synthesis, structure-properties relations, advanced and functional materials with designed properties, (macro)molecular architecture, material engineering

Host institution :

Uniwersytet Warszawski, Wydział Fizyki

woj. mazowieckie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr hab. Joanna Kowalska 

Number of co-investigators in the project: 8

Call: SONATA BIS 5 - announced on 2015-06-15

Amount awarded: 1 993 800 PLN

Project start date (Y-m-d): 2016-04-12

Project end date (Y-m-d): 2021-10-11

Project duration:: 66 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. System FPLC do oczyszczania białek (280 000 PLN)
  2. Termocykler (30 000 PLN)
  3. Termomikser (15 000 PLN)
  4. System do chromatografii cieczowej (flash) (260 000 PLN)

Information in the final report

  • Publication in academic press/journals (9)
  • Articles in post-conference publications (7)
  1. Direct High-Throughput Screening Assay for mRNA Cap Guanine-N7 Methyltransferase Activity
    Authors:
    Renata Kasprzyk, Mateusz Fido, Adam Mamot, Przemysław Wanat, Mirosław Śmietański, Michał Kopciał, Victoria Cowling, Joanna Kowalska, Jacek Jemielity
    Academic press:
    Chemistry - A European Journal (rok: 2020, tom: 26, strony: 11266-11275), Wydawca: Wiley-VCH GmbH
    Status:
    Published
    DOI:
    10.1002/chem.202001036 - link to the publication
  2. ExciTides: NTP-derived probes for monitoring pyrophosphatase activity based on excimer-to-monomer transitions
    Authors:
    Przemyslaw Wanat, Renata Kasprzyk, Michal Kopcial, Pawel J. Sikorski, Dominika Strzelecka, Jacek Jemielity and Joanna Kowalska*
    Academic press:
    Chemical Communications (rok: 2018, tom: 54, strony: 9773-9776), Wydawca: Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/C8CC04968H - link to the publication
  3. Nicotinamide-Containing Di- and Trinucleotides as Chemical Tools for Studies of NAD-Capped RNAs
    Authors:
    Agnieszka Mlynarska-Cieslak, Anais Depaix, Ewa Grudzien-Nogalska, Pawel J. Sikorski, Marcin Warminski, Megerditch Kiledjian, Jacek Jemielity, and Joanna Kowalska*
    Academic press:
    Organic Letters (rok: 2018, tom: 20, strony: 7650–7655), Wydawca: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/acs.orglett.8b03386 - link to the publication
  4. DXO/Rai1 enzymes remove 5′-end FAD and dephospho-CoA caps on RNAs
    Authors:
    Selom K. Doamekpor, Ewa Grudzień-Nogalska, Agnieszka Młynarska-Cieślak, Joanna Kowalska, Megerditch Kiledjian, Liang Tong
    Academic press:
    Nucleic Acids Research (rok: 2020, tom: 48, strony: 6136–6148), Wydawca: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/nar/gkaa297 - link to the publication
  5. Purine and pyrimidine dinucleoside polyphosphates differentially affect the phenylpropanoid pathway in Vitis vinifera L. cv. Monastrell suspension cultured cells
    Authors:
    Małgorzata Pietrowska-Borek, Anna Wojdyła-Mamoń, Jędrzej Dobrogojski, Agnieszka Młynarska-Cieślak, Marek R Baranowski, Jakub M. Dąbrowski, Joanna Kowalska, Jacek Jemielity, Sławomir Borek, Maria Angeles Pedreño, Andrzej Guranowski
    Academic press:
    Plant Physiology and Biochemistry (rok: 2020, tom: 147, strony: 125-132), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.plaphy.2019.12.015 - link to the publication
  6. RNA Ligation for Mono and Dually Labeled RNAs
    Authors:
    Anaïs Depaix, Agnieszka Mlynarska-Cieslak, Marcin Warminski, Pawel J. Sikorski, Jacek Jemielity, Joanna Kowalska
    Academic press:
    Chemistry - A European Journal (rok: 2021, tom: 27, strony: 12190-12197), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/chem.202101909 - link to the publication
  7. Fluorinated PAPS analogs for continuous sulfotransferase activity monitoring and inhibitor screening by 19F NMR spectroscopy
    Authors:
    Agnieszka Mlynarska-Cieslak, Mikolaj Chrominski, Tomasz Spiewla, Marek R. Baranowski, Marcelina Bednarczyk, Jacek Jemielity, Joanna Kowalska*
    Academic press:
    ACS Chemical Biology (rok: 2022, tom: 17, strony: 661–669), Wydawca: ACS
    Status:
    Published
    DOI:
    10.1021/acschembio.1c00978 - link to the publication
  8. Preparation of RNAs with non-canonical 5′ ends using novel di- and trinucleotide reagents for co-transcriptional capping
    Authors:
    Anaïs Depaix, Ewa Grudzien-Nogalska, Bartlomiej Fedorczyk, Megerditch Kiledjian, Jacek Jemielity,* Joanna Kowalska *
    Academic press:
    Frontiers in Molecular Biosciences , Wydawca: Frontiers
    Status:
    Submitted
    DOI:
    brak - link to the publication
  9. NAD Analogs in Aid of Chemical Biology and Medicinal Chemistry
    Authors:
    Anais Depaix, Joanna Kowalska
    Academic press:
    Molecules (rok: 2019, tom: 24, strony: 4187), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/molecules24224187 - link to the publication
  1. New NAD-capped RNA analogs as chemical tools to study RNA deNADing
    Authors:
    Anais Depaix, Agnieszka Młynarska, Paweł Sikorski, Jacek Jemielity, Joanna Kowalska
    Conference:
    61. Zjazd Naukowy Polskiego Towarzystwa Chemicznego (rok: 2018, ), Wydawca: Polskie Towarzystwo Chemiczne
    Data:
    konferencja 17-21.09.2018
    Status:
    Published
  2. A chemical toolbox to study NAD-capped RNAs
    Authors:
    Mlynarska-Cieslak A., Depaix A., Grudzien-Nogalska E., Sikorski P., Warminski M., Kiledjian M., Jemielity J., Kowalska J.
    Conference:
    24th Annual Meeting of the RNA Society (rok: 2019, ), Wydawca: RNA Society
    Data:
    konferencja 11-16.06.2019
    Status:
    Published
  3. Phosphate-modified nucleotides as molecular tools for probing and modulating protein activity
    Authors:
    Joanna Kowalska, Jacek Jemielity
    Conference:
    XXIII INTERNATIONAL ROUNDTABLE ON NUCLEOSIDES, NUCLEOTIDES AND NUCLEIC ACIDS (rok: 2018, ), Wydawca: University of California San Diego
    Data:
    konferencja 26-30.08.2018
    Status:
    Published
  4. Exploring fragmentation pathways for dinucleotides using tandem mass spectrometry
    Authors:
    Agnieszka Brzezińska, Dominika Strzelecka, Sebastian Chmieliński, Sylwia Bednarek, Jacek Jemielity, Joanna Kowalska
    Conference:
    6th Conference of Polish Mass Spectrometry Society (rok: 2018, ), Wydawca: Institute of Organic Chemistry, Polish Academy of Sciences
    Data:
    konferencja 23-26.04.2018
    Status:
    Published
  5. Phosphate modified PAPS analogs as tools to study sulfotransferases
    Authors:
    Agnieszka Młynarska-Cieślak, Marek R. Baranowski, Dorota Kubacka, Marcin Warmiński, Jacek Jemielity, Joanna Kowalska
    Conference:
    XXIII INTERNATIONAL ROUNDTABLE ON NUCLEOSIDES, NUCLEOTIDES AND NUCLEIC ACIDS (rok: 2018, ), Wydawca: University of California San Diego
    Data:
    konferencja 26-30.08.2018
    Status:
    Published
  6. Synteza analogów PAPS jako inhibitorów sulfotransferaz i ich ewaluacja przy użyciu metody MST
    Authors:
    Młynarska-Cieślak A., Baranowski M., Kubacka D., Warmiński M., Magda M., Jemielity J., Kowalska J.
    Conference:
    62 Zjazd Naukowy Polskiego Towarzystwa Chemicznego (rok: 2019, ), Wydawca: Polskie Towarzystwo Chemiczne
    Data:
    konferencja 02-06.09.2019
    Status:
    Published
  7. Synteza nowych analogów PAPS jako inhibitorów dla sulfotransferaz
    Authors:
    Agnieszka Młynarska-Cieślak, Dorota Kubacka, Marek Baranowski, Marcin Warmiński, Jacek Jemielity, Joanna Kowalska
    Conference:
    61. Zjazd Naukowy Polskiego Towarzystwa Chemicznego (rok: 2018, ), Wydawca: Polskie Towarzystwo Chemiczne
    Data:
    konferencja 17-21.09.2018
    Status:
    Published

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