Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Bronchial fibroblast to myofibroblast transition - the role of differential activation of TGF-beta/Smad signalling pathway in cells derived from asthmatic patients and non-asthmatic donors - in vitro studies in 2D,3D and EMTU models.

2015/17/B/NZ3/02248

Keywords:

fibroblast to myofibroblast transition (FMT) transforming growth factor beta (TGF-beta) TGF-beta/Smad signalling pathway bronchial asthma 3D cultures epithelial-mesenchymal trophic unit (EMTU)

Descriptors:

  • NZ3_1: Cell biology
  • NZ3_2: Cell physiology
  • NZ3_7: Signal transduction

Panel:

NZ3 - Cellular and developmental biology: cell biology, developmental biology, ageing biology, neurobiology

Host institution :

Uniwersytet Jagielloński, Wydział Biochemii, Biofizyki i Biotechnologii

woj. małopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr hab. Marta Michalik 

Number of co-investigators in the project: 7

Call: OPUS 9 - announced on 2015-03-16

Amount awarded: 952 000 PLN

Project start date (Y-m-d): 2016-02-18

Project end date (Y-m-d): 2019-12-17

Project duration:: 46 months (the same as in the proposal)

Project status: Project settled

Project description

Download the project description in a pdf file

Note - project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. Kość Filtrowa Alexa 594 do mikroskopu fluorescencyjnego Leica DMI6000B (7 000 PLN)
  2. Woltometr do hodowli komórkowych (9 000 PLN)
  3. Kość Filtrowa Alexa 647 (Y5) do mikroskopu fluorescencyjnego Leica DMI6000B (7 000 PLN)
  4. Zestaw do immunoblotingu (Western blot) (20 000 PLN)
  5. Inkubator do hodowli komórkowych.

Information in the final report

  • Publication in academic press/journals (7)
  • Articles in post-conference publications (5)
  1. Responsiveness of human bronchial fibroblasts and epithelial cells from asthmatic and non-asthmatic donors to the transforming growth factor-β1 in epithelial-mesenchymal trophic unit model.
    Authors:
    Paw M, Wnuk D, Jakieła B, Bochenek G, Sładek K, Madeja Z, Michalik M.
    Academic press:
    BMC Molecular and Cell Biology (rok: 2021, tom: 22, strony: 19), Wydawca: Springer Nature
    Status:
    Published
    DOI:
    10.1186/s12860-021-00356-8 - link to the publication
  2. Enhanced asthma-related fibroblast to myofibroblast transition is the result of profibrotic TGF-ß/Smad2/3 pathway intensification and antifibrotic TGF-ß/Smad1/5/(8)9 pathway impairment
    Authors:
    Dawid Wnuk, Milena Paw, Karolina Ryczek, Grażyna Bochenek, Krzysztof Sładek, Zbigniew Madeja, Marta Michalik
    Academic press:
    Scientific Reports (rok: 2020, tom: 10, strony: 16492), Wydawca: Nature Research
    Status:
    Published
    DOI:
    10.1038/s41598-020-73473-7 - link to the publication
  3. Pentoxifylline and its active metabolite lisofylline attenuate transforming growth factor β1-induced asthmatic bronchial fibroblast-to-myofibroblast transition
    Authors:
    Wójcik-Pszczoła K, Hińcza K, Wnuk D, Kądziołka D, Koczurkiewicz P, Sanak M, Madeja Z, Pękala E, Michalik M
    Academic press:
    Acta Biochim Pol. (rok: 2016, tom: 63(3), strony: 437-42), Wydawca: Polish Biochemical Society and of the Committee of Biochemistry and Biophysics Polish Academy of Sciences
    Status:
    Published
    DOI:
    10.18388/abp.2016_1357 - link to the publication
  4. Fibroblast to myofibroblast transition is enhanced in asthma derived fibroblasts in comparison to fibroblasts derived from non-asthmatic patients in 3D in vitro cultures due to Smad2/3 signalling.
    Authors:
    Wnuk D, Lasota S, Paw M, Madeja Z, Michalik M.
    Academic press:
    Acta Biochim Pol. (rok: 2020, tom: 67, strony: 441-448), Wydawca: PTBioch & PAN
    Status:
    Published
    DOI:
    10.18388/abp.2020_5412 - link to the publication
  5. Fibroblast-to-myofibroblast transition in bronchial asthma.
    Authors:
    Michalik M, Wójcik-Pszczoła K, Paw M, Wnuk D, Koczurkiewicz P, Sanak M, Pękala E, Madeja Z.
    Academic press:
    Cell Mol Life Sci (rok: 2018, tom: 75, strony: 3943–3961), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s00018-018-2899-4 - link to the publication
  6. Connexin43 Controls the Myofibroblastic Differentiation of Bronchial Fibroblasts from Asthmatic Patients.
    Authors:
    Paw M, Borek I, Wnuk D, Ryszawy D, Piwowarczyk K, Kmiotek K, Wójcik-Pszczoła KA, Pierzchalska M, Madeja Z, Sanak M, Błyszczuk P, Michalik M, Czyż J.
    Academic press:
    Am J Respir Cell Mol Biol (rok: 2017, tom: 57(1), strony: 100-110), Wydawca: AJRCCM
    Status:
    Published
    DOI:
    10.1165/rcmb.2015-0255OC - link to the publication
  7. Fenofibrate Reduces the Asthma-Related Fibroblast-To-Myofibroblast Transition by TGF-β/Smad2/3 Signaling Attenuation and Connexin 43-Dependent Phenotype Destabilization
    Authors:
    Paw M, Wnuk D, Kądziołka D, Sęk A, Lasota S, Czyż J, Madeja Z, Michalik M.
    Academic press:
    Int J Mol Sci (rok: 2018, tom: 19(9), strony: 2571. pii: E2571), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms19092571 - link to the publication
  1. Endocytosis may be involved in asthma development by regulation of TGF-β dependent human bronchial fibroblasts to miofibroblast transition
    Authors:
    Ryszawy D,Wnuk D, Karnas E, Paw M, Madeja Z, Michalik M.
    Conference:
    21st Congress of the APSR 2016, Bangkok, Thailand (rok: 2016, ), Wydawca: WILEY, RESPIROLOGY 21, Supplement 3
    Data:
    konferencja 12–15 November
    Status:
    Published
  2. Enhanced efficiency of phenotypic transition of asthmatic fibroblasts depends on canonical and non-canonical TGF- β/Smad signaling pathways
    Authors:
    Wnuk D, Ryczek K, Paw M, Kmiotek-Wasylewska K, Sładek K, Bochenek G, Michalik M.
    Conference:
    28th International Congress of the European-Respiratory-Society (ERS), Paris, FRANCE (rok: 2018, ), Wydawca: ERS; EUROPEAN RESPIRATORY JOURNAL 52; Suppl. 62
    Data:
    konferencja SEP 15-19
    Status:
    Published
  3. Increased endocytosis activity contributes to the enhanced TGF- β-induced fibroblast to myofibroblast transition in bronchial asthma
    Authors:
    Ryszawy D, Wnuk D, Janik N, Karnas E, Paw M, Plutecka H, Jakieła B, Madeja Z, Michalik M.
    Conference:
    28th International Congress of the European-Respiratory-Society (ERS), Paris, FRANCE (rok: 2018, ), Wydawca: ERS; EUROPEAN RESPIRATORY JOURNAL 52,Suppl. 62
    Data:
    konferencja SEP 15-19
    Status:
    Published
  4. Fenofibrate-induced inhibition of TGF- β/Smad signalling pathway attenuates fibroblast-to-myofibroblast transition in bronchial fibroblasts derived from asthmatic patients
    Authors:
    Wnuk D, Paw M, Kądziołka D, Sęk A, Jeż D, Kmiotek-Wasylewska K, Michalik M.
    Conference:
    42nd Congress of the Federation-of-European-Biochemical-Societies (FEBS) on From Molecules to Cells and Back; Jerusalem, ISRAEL (rok: 2017, ), Wydawca: FEBS PRESS; The FEBS Journal, 284, Supplement: 1
    Data:
    konferencja SEP 10-14,
    Status:
    Published
  5. Inhibition of RhoA pathway decreases formin-related gene expression and TGF-β1-induced fibroblast to myofibroblast transition of bronchial fibroblasts derived from asthmatic patients.
    Authors:
    Wnuk D, Kmiotek K, Ryszawy D, Paw M, Michalik M.
    Conference:
    21st Congress of the APSR 2016, Bangkok, Thailand (rok: 2016, ), Wydawca: WILEY, RESPIROLOGY, 21, Supplement: 3
    Data:
    konferencja 12–15 November
    Status:
    Published

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