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Insight into biochemistry, structure, evolution and biology of bacterial serpins on example of miropin from human pathogen Tannerella forsythia.

2015/17/B/NZ1/00666

Keywords

Descriptors

  • NZ1_2: Biochemia
  • NZ1_1: Biologia molekularna

Panel

NZ1 – Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution

Uniwersytet Jagielloński, Małopolskie Centrum Biotechnologii Uniwersytetu Jagiellońskiego

Voivodeship: małopolskie

Other projects carried out by the institution »

Principal investigator (from the host institution)

dr Mirosław Książek

Number of co-investigators in the project: 3

Call

OPUS 9 – announced on 2015-03-16

Amount awarded
1 255 700 PLN
Project start date
2016-02-04
Project end date
2019-12-03
Project duration
46 months (the same as in the proposal)
Project status
Project settled

Project description

Download the project description in a pdf file

Note — project descriptions were prepared by the authors of the applications themselves and placed in the system in an unchanged form.

Equipment purchased [PL]

  1. Horyzontalny zestaw do elektroforezy żeli agarozowych. Cost: 2 400 PLN
  2. Komputer niestacjonarny typu laptop.
  3. Lodówka. Cost: 6 000 PLN
  4. Mała cieplarka laboratoryjna. Cost: 5 750 PLN
  5. Mieszadło magentyczne (3 pcs). Cost: 7 950 PLN
  6. Oprogramowanie do analizy sekwencji DNA Lasergene (DNAStar).
  7. Poręczny homogenizator laboratoryjny. Cost: 10 500 PLN
  8. Prosty worteks laboratoryjny (2 pcs). Cost: 1 600 PLN
  9. Spektrofotometryczny czytnik mikropłytek z laptopem sterującym.
  10. Termoblok z wymiennymi blokami grzejnymi. Cost: 7 000 PLN
  11. Termomikser z blokami na probówki 1.5, 2 ml i płytki. Cost: 19 000 PLN
  12. Wirówka z zestawem rotorów. Cost: 60 000 PLN
  13. Zamrażarka -20oC. Cost: 5 000 PLN
  14. Zestaw do elektroforezy w żelach poliakrylamidowych (2 pcs). Cost: 18 500 PLN
  15. Zestaw do elektrotransferu "mokrego" (2 pcs). Cost: 14 000 PLN

Information in the final report

Publication in academic press/journals (6)

  1. Plasmin inhibition by bacterial serpin: Implications in gum disease.

    Authors:
    Sochaj-Gregorczyk A, Ksiazek M (równorzędny I autor; autor korespondencyjny), Waligorska I, Straczek A, Benedyk M, Mizgalska D, Thøgersen IB, Enghild JJ, Potempa J
    Academic press:
    FASEB J (year: 2020, volume: 34, pages: 619-630), Publisher: Wiley
    Status:
    Published
    DOI:
    10.1096/fj.201901490RR – link to the publication

  2. Structure-based mechanism of cysteine-switch latency and of catalysis by pappalysin-family metallopeptidases

    Authors:
    Tibisay Guevara, Arturo Rodriguez-Banqueri, Miroslaw Ksiazek, Jan Potempa, F. Xavier Gomis-Rüth
    Academic press:
    IUCrJ (year: 2020, volume: 7, pages: 18-29), Publisher: International Union of Crystallography (IUCr)
    Status:
    Published
    DOI:
    10.1107/S2052252519013848 – link to the publication

  3. Structural determinants of inhibition of Porphyromonas gingivalis gingipain K by KYT-36, a potent, selective, and bioavailable peptidase inhibitor.

    Authors:
    Guevara T, Rodríguez-Banqueri A, Lasica AM, Ksiazek M, Potempa BA, Potempa J, Gomis-Rüth FX
    Academic press:
    Scientific reports (year: 2019, volume: 9, pages: 4935), Publisher: Nature Publishing Group
    Status:
    Published
    DOI:
    10.1038/s41598-019-41354-3 – link to the publication

  4. Mirolysin, a LysargiNase from Tannerella forsythia, proteolytically inactivates the human cathelicidin, LL-37.

    Authors:
    Lahari Koneru, Miroslaw Ksiazek (równorzędny I autor; autor korespondencyjny), Irena Waligorska, Anna Straczek, Magdalena Lukasik, Mariusz Madej, Ida B. Thøgersen, Jan J. Enghild and Jan Potempa
    Academic press:
    Biological Chemistry (year: 2017, volume: 398(3), pages: 365-409), Publisher: De Gruyter
    Status:
    Published
    DOI:
    10.1515/hsz-2016-0267 – link to the publication

  5. Structural and functional probing of PorZ, an essential bacterial surface component of the type-IX secretion system of human oral-microbiomic Porphyromonas gingivalis.

    Authors:
    Lasica AM, Goulas T, Mizgalska D, Zhou X, de Diego I, Ksiazek M, Madej M, Guo Y, Guevara T, Nowak M, Potempa B, Goel A, Sztukowska M, Prabhakar AT, Bzowska M, Widziolek M, Thøgersen IB, Enghild JJ, Simonian M, Kulczyk AW, Nguyen KA, Potempa J, Gomis-Rüth FX.
    Academic press:
    Scientific reports (year: 2016, volume: 6, pages: 37708), Publisher: Nature Publishing Group
    Status:
    Published
    DOI:
    10.1038/srep37708 – link to the publication

  6. A structure-derived snap-trap mechanism of a multispecific serpin from the dysbiotic human oral microbiome

    Authors:
    Goulas T, Ksiazek M (równorzędny I autor), Garcia-Ferrer I, Sochaj-Gregorczyk AM, Waligorska I, Wasylewski M, Potempa J, Gomis-Rüth FX
    Academic press:
    Journal of Biological Chemistry (year: 2017, volume: 292(26), pages: 10883-10898), Publisher: American Society for Biochemistry and Molecular Biology
    Status:
    Published
    DOI:
    10.1074/jbc.M117.786533 – link to the publication

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