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Information on the principal investigator and host institution

Information of the project and the call

Keywords

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Mechanisms underlying the targeting of coding sequence repeats by microRNA-like inhibitors

2014/15/B/NZ1/01880

Keywords:

polyglutamine diseases CAG repeat targeting silencing mechanisms miRNA HITS-CLIP ribosome profiling

Descriptors:

  • NZ1_1: Molecular biology
  • NZ2_3: Proteomics
  • NZ2_2: Genomics, transcriptomics, epigenomics

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

Instytut Chemii Bioorganicznej PAN

woj. wielkopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

dr Agnieszka Fiszer 

Number of co-investigators in the project: 6

Call: OPUS 8 - announced on 2014-09-15

Amount awarded: 1 476 600 PLN

Project start date (Y-m-d): 2015-07-15

Project end date (Y-m-d): 2019-07-14

Project duration:: 48 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Sonikator.
  2. Komputer z oprogramowaniem (3 szt.) (15 000 PLN)
  3. Mini wirówki 2 sztuki.
  4. Pompa do odsysania płynów przy hodowlach komórkowych (5 000 PLN)
  5. Analizator RNA, DNA i białek. (100 000 PLN)
  6. Aparat do elektroforezy i transferu białek (10 000 PLN)
  7. Kolektor frakcji (15 000 PLN)
  8. Licznik komórek (15 000 PLN)
  9. Termomikser (5 000 PLN)
  10. Zamrażarka niskotemperaturowa -80st.C (35 000 PLN)

Information in the final report

  • Publication in academic press/journals (9)
  • Articles in post-conference publications (1)
  1. Artificial miRNAs targeting CAG repeat expansion in ORFs cause rapid deadenylation and translation inhibition of mutant transcripts
    Authors:
    Adam Ciesiolka, Anna Stroynowska-Czerwinska, Paweł Joachimiak, Agata Ciolak, Emilia Kozlowska, Michal Michalak, Magdalena Dabrowska, Marta Olejniczak, Katarzyna D Raczynska, Dominika Zielinska, Magdalena Wozna-Wysocka, Wlodzimierz J Krzyzosiak, Agnieszka Fiszer
    Academic press:
    Cellular and Molecular Life Sciences (rok: 2021, tom: 78, strony: 1577-1596), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s00018-020-03596-7 - link to the publication
  2. Reduction of Huntington's Disease RNA Foci by CAG Repeat-Targeting Reagents
    Authors:
    Martyna O. Urbanek, Agnieszka Fiszer, Wlodzimierz J. Krzyzosiak
    Academic press:
    Frontiers in Cellular Neuroscience (rok: 2017, tom: 11, strony: 82), Wydawca: FRONTIERS MEDIA SA
    Status:
    Published
    DOI:
    10.3389/fncel.2017.00082 - link to the publication
  3. siRNA release from pri-miRNA scaffolds is controlled by the sequence and structure of RNA.
    Authors:
    Galka-Marciniak P, Olejniczak M, Starega-Roslan J, Szczesniak MW, Makalowska I, Krzyzosiak WJ
    Academic press:
    Biochimica et Biophysica Acta-Gene Regulatory Mechanisms (rok: 2016, tom: 1859, strony: 639-49), Wydawca: ELSEVIER SCIENCE BV
    Status:
    Published
    DOI:
    10.1016/j.bbagrm.2016.02.014 - link to the publication
  4. A potential role of extended simple sequence repeats in competing endogenous RNA crosstalk
    Authors:
    Tomasz M. Witkoś, Włodzimierz J. Krzyżosiak, Agnieszka Fiszer, Edyta Kościańska
    Academic press:
    RNA Biology (rok: 2018, tom: 15, strony: 1399-1409), Wydawca: Talor&Francis
    Status:
    Published
    DOI:
    10.1080/15476286.2018.1536593 - link to the publication
  5. Mutant CAG Repeats Effectively Targeted by RNA Interference in SCA7 Cells.
    Authors:
    Fiszer A, Wroblewska JP, Nowak BM, Krzyzosiak WJ
    Academic press:
    Genes (Basel) (rok: 2016, tom: 7, strony: 132), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/genes7120132 - link to the publication
  6. Nucleotide sequence of miRNA precursor contributes to cleavage site selection by Dicer.
    Authors:
    Starega-Roslan J, Galka-Marciniak P, Krzyzosiak WJ
    Academic press:
    Nucleic Acids Research (rok: 2015, tom: 43, strony: 10939-51), Wydawca: OXFORD UNIVERSITY PRESS
    Status:
    Published
    DOI:
    10.1093/nar/gkv968 - link to the publication
  7. RNA FISH for detecting expanded repeats in human diseases.
    Authors:
    Urbanek MO, Krzyzosiak WJ
    Academic press:
    Methods (rok: 2016, tom: 98, strony: 115-23), Wydawca: ELSEVIER- Academic Press INC Elsevier Science
    Status:
    Published
    DOI:
    10.1016/j.ymeth.2015.11.017 - link to the publication
  8. Silencing of genes responsible for polyQ diseases using chemically modified single-stranded siRNAs.
    Authors:
    Fiszer A, Ellison-Klimontowicz ME, Krzyzosiak WJ
    Academic press:
    Acta Biochimica Polonica (rok: 2016, tom: 63, strony: 759-764), Wydawca: Polish Biochemical Society
    Status:
    Published
    DOI:
    10.18388/abp.2016_1336 - link to the publication
  9. Identifying proteins that bind to specific RNAs - focus on simple repeat expansion diseases.
    Authors:
    Jazurek M, Ciesiolka A, Starega-Roslan J, Bilinska K, Krzyzosiak WJ
    Academic press:
    Nucleic Acids Research (rok: 2016, tom: 44, strony: 9050-9070), Wydawca: OXFORD UNIVERSITY PRESS
    Status:
    Published
    DOI:
    10.1093/nar/gkw803 - link to the publication
  1. Mutant HTT mRNA as therapeutic target in allele-selective CAG repeat-directed RNAi approach and putative pathogenic agent in HD
    Authors:
    Agnieszka Fiszer, Łukasz Przybył, Magdalena Jazurek-Ciesiołka, Adam Ciesiołka, Paweł Joachimiak, Emilia Kozłowska, Michał Michalak, Paweł M. Świtoński, Magdalena Woźna-Wysocka, Agata Łużna, Włodzimierz J. Krzyżosiak
    Conference:
    EHDN Plenary Meeting (rok: 2018, ), Wydawca: Journal of Neurology Neurosurgery and Psychiatry
    Data:
    konferencja 14-16.09.2018
    Status:
    Published
    DOI:
    link to the publication

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