Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Next-generation sequencing of miRNA transcriptome and functional in vitro study: towards unravelling pathomechanisms and heterogeneity of pediatric T-cell acute lymphoblastic leukemia (T-ALL)

2014/15/B/NZ2/03394

Keywords:

T-cell acute lymphoblastic leukemia miRNA next-generation sequencing (miRNA-seq) functional in vitro study - miRNA inhibition/mimics bioinformatics prediction and in vitro modulation of target mRNA

Descriptors:

  • NZ2_1: Molecular genetics
  • NZ2_2: Genomics, transcriptomics, epigenomics
  • NZ5_3: Pathogenesis of human diseases

Panel:

NZ2 - Genetics, genomics: molecular genetics, genomics, proteomics, bioinformatics, systems biology, genetic epidemiology

Host institution :

Instytut Genetyki Człowieka PAN

woj. wielkopolskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Michał Witt 

Number of co-investigators in the project: 11

Call: OPUS 8 - announced on 2014-09-15

Amount awarded: 1 395 010 PLN

Project start date (Y-m-d): 2015-08-05

Project end date (Y-m-d): 2021-02-04

Project duration:: 66 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Inkubator.
  2. Laptop z oprogramowaniem (7 000 PLN)
  3. Lodowka (2 000 PLN)
  4. Wirówka.
  5. Cycler (40 000 PLN)

Information in the final report

  • Publication in academic press/journals (10)
  • Book publications / chapters in book publications (1)
  1. CRISPRi for specific inhibition of miRNA clusters and miRNAs with high sequence homology
    Authors:
    Monika Drobna-Śledzińska, Natalia Maćkowska-Maślak, Roman Jaksik, Paulina Dąbek, Michał Witt & Małgorzata Dawidowska
    Academic press:
    Scientific Reports (rok: 2022, tom: 12(1), strony: 6297 (nr artykułu)), Wydawca: Nature
    Status:
    Published
    DOI:
    10.1038/s41598-022-10336-3. - link to the publication
  2. Comprehensive investigation of miRNome identifies novel candidate miRNA-mRNA interactions implicated in T-cell acute lymphoblastic leukemia
    Authors:
    Dawidowska M, Jaksik R, Drobna M, Szarzyńska-Zawadzka B, Kosmalska M, Sędek Ł, Machowska L, Lalik A, Lejman M, Ussowicz M, Kałwak K, Kowalczyk JR, Szczepański T, Witt M.
    Academic press:
    Neoplasia (rok: 2019, tom: 21, strony: 294-310), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.neo.2019.01.004 - link to the publication
  3. Translocation t(8;14)(q24;q11) with concurrent PTEN alterations and deletions of STIL/TAL1 and CDKN2A/B in a pediatric case of acute T-lymphoblastic leukemia: A genetic profile associated with adverse prognosis
    Authors:
    Skalska-Sadowska J, Dawidowska M, Szarzyńska-Zawadzka B, Jarmuż-Szymczak M, Czerwińska-Rybak J, Machowska L, Derwich K.
    Academic press:
    Pediatr Blood Cancer (rok: 2017, tom: 64(4), strony: e26266), Wydawca: Wiley Periodicals, Inc.
    Status:
    Published
    DOI:
    10.1002/pbc.26266 - link to the publication
  4. DNA Methylation in T-Cell Acute Lymphoblastic Leukemia: In Search for Clinical and Biological Meaning
    Authors:
    Maćkowska M, Drobna-Śledzińska M, Witt M, Dawidowska M
    Academic press:
    International Journal of Molecular Sciences (rok: 2021, tom: 22, strony: brak (tylko online)), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms22031388 - link to the publication
  5. RNA-seq library preparation for comprehensive transcriptome analysis in cancer cells: The impact of insert size
    Authors:
    Roman Jaksik, Monika Drobna-Śledzińska, Małgorzata Dawidowska
    Academic press:
    Genomics (rok: 2021, tom: 113, strony: 4149-4162), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.018 - link to the publication
  6. T-cell acute lymphoblastic leukemia from miRNA perspective: basic concepts, experimental approaches, and potential biomarkers
    Authors:
    Monika Drobna, Bronisława Szarzyńska-Zawadzka, Małgorzata Dawidowska
    Academic press:
    Blood Reviews (rok: 2018, tom: 32, strony: 457-472), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.blre.2018.04.003 - link to the publication
  7. hsa-miR-20b-5p and hsa-miR-363-3p Affect Expression of PTEN and BIM Tumor Suppressor Genes and Modulate Survival of T-ALL Cells In Vitro
    Authors:
    Drobna M, Szarzyńska B, Jaksik R, Sędek Ł, Kuchmiy A, Taghon T, Van Vlierberghe P, Szczepański T, Witt M, Dawidowska M
    Academic press:
    Cells (rok: 2020, tom: 9, strony: brak (tylko online)), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/cells9051137 - link to the publication
  8. Identification of Endogenous Control miRNAs for RT-qPCR in T-Cell Acute Lymphoblastic Leukemia
    Authors:
    Drobna M, Szarzyńska-Zawadzka B, Daca-Roszak P, Kosmalska M, Jaksik R, Witt M, Dawidowska M.
    Academic press:
    Int J Mol Sci (rok: 2018, tom: 19, strony: 45308), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms19102858 - link to the publication
  9. Small RNA-Seq Reveals Similar miRNA Transcriptome in Children and Young Adults with T-ALL and Indicates miR-143-3p as Novel Candidate Tumor Suppressor in This Leukemia.
    Authors:
    Dawidowska M, Maćkowska-Maślak N, Drobna-Śledzińska M, Kosmalska M, Jaksik R, Szymczak D, Jarmuż-Szymczak M, Sadowska-Klasa A, Wojtaszewska M, Sędek Ł, Wróbel T, Zaucha JM, Szczepański T, Lewandowski K, Giebel S, Witt M.
    Academic press:
    Int J Mol Sci. (rok: 2022, tom: 23(17), strony: 10117 (nr artykułu)), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms231710117 - link to the publication
  10. Multiomics to investigate the mechanisms contributing to repression of PTPRC and SOCS2 in pediatric T-ALL: Focus on miR-363-3p and promoter methylation
    Authors:
    Drobna-Śledzińska M, Maćkowska-Maślak N, Jaksik R, Kosmalska M, Szarzyńska B, Lejman M, Sędek Ł, Szczepański T, Taghon T, Van Vlierberghe P, Witt M, Dawidowska M.
    Academic press:
    Genes Chromosomes Cancer (rok: 2022, tom: 61(12), strony: 720-733), Wydawca: Wiley
    Status:
    Published
    DOI:
    10.1002/gcc.23085 - link to the publication
  1. Molecular biology of leukaemia
    Authors:
    Małgorzata Dawidowska, Michał Witt
    Book:
    Leukaemia & Myeloma: Essentials for Clinicians (rok: 2019, tom: nie dotyczy, strony: 63-66), Wydawca: ESMO Press
    Status:
    Published

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