Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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From a molecular in silico model to cellular response in vitro. Modified nucleosides as photo and/or radiosensitizers of DNA damage

2014/14/A/ST4/00405

Keywords:

modified nucleosides rdiosensitizers photosensitizers DNA double strand breaks cellular response anticancer therapy

Descriptors:

  • ST4_17: Chemical reactions: mechanisms, thermodynamics, kinetics
  • ST4_15: Colloid chemistry
  • ST4_16: Ionic liquids

Panel:

ST4 - Chemistry: physical chemistry/chemical physics, theoretical chemistry, analytical chemistry, inorganic chemistry, organic chemistry, method development

Host institution :

Uniwersytet Gdański, Wydział Chemii

woj. pomorskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Janusz Rak 

Number of co-investigators in the project: 21

Call: MAESTRO 6 - announced on 2014-06-16

Amount awarded: 3 958 291 PLN

Project start date (Y-m-d): 2015-04-23

Project end date (Y-m-d): 2021-01-22

Project duration:: 69 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Aparat do pomiarów stężenia tlenu w kulturach komórkowych (158 000 PLN)
  2. Preparatywny system HPLC (251 000 PLN)
  3. System UHPLC (362 000 PLN)
  4. System do liofilizacji z rozpuszczalników niewodnych.
  5. system do oczyszczania wody.
  6. Komora do naświetlań UV (100 000 PLN)
  7. System do analizy Western blot (12 500 PLN)
  8. Inkubator z kontrolą ciśnienia tlenu (37 000 PLN)
  9. Odwrócony mikroskop fluorescencyjny (122 000 PLN)
  10. System elektroforetyczny do sekwencjonowania DNA (17 000 PLN)
  11. Wirówka z chłodzeniem (16 000 PLN)
  12. Cytometr przepływowy (264 450 PLN)
  13. Klaster komputerowy (350 000 PLN)
  14. Komora do napromieniań promieniowaniem X (320 000 PLN)
  15. Niskotemperaturowa zamrażarka (26 000 PLN)

Information in the final report

  • Publication in academic press/journals (16)
  • Book publications / chapters in book publications (2)
  1. Molecular features of thymidine analogues governing the activity of human thymidine kinase
    Authors:
    Karolina Jagiello, Samanta Makurat, Sylwester Pereć, Janusz Rak, Tomasz Puzyn
    Academic press:
    Structural Chemistry (rok: 2018, tom: 29, strony: 1367–1374), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s11224-018-1124-2 - link to the publication
  2. The Sequence Dependence of Photoinduced Single Strand Break in 5-Bromo-2′-deoxyuridine Labeled DNA Supports That Electron Transfer Is Responsible for the Damage
    Authors:
    Kinga Westphal, Samanta Makurat, Janusz Rak
    Academic press:
    The Journal of Physical Chemistry B (rok: 2017, tom: 121, strony: 9169−9174), Wydawca: American Chemical Socitey
    Status:
    Published
    DOI:
    10.1021/acs.jpcb.7b07338 - link to the publication
  3. 5-(N-Trifluoromethylcarboxy)aminouracil as a Potential DNA Radiosensitizer and Its Radiochemical Conversion into N-Uracil-5-yloxamic Acid
    Authors:
    Paulina Spisz, Witold Kozak, Lidia Chomicz-Mańka, Samanta Makurat, Karina Falkiewicz, Artur Sikorski, Anna Czaja, Janusz Rak, Magdalena Zdrowowicz
    Academic press:
    International Journal o f Molecular Sciences (rok: 2020, tom: 21, strony: 6352), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms21176352 - link to the publication
  4. 5-Selenocyanatouracil: A Potential Hypoxic Radiosensitizer. Electron Attachment Induced Formation of Selenium Centered Radical
    Authors:
    Marta Sosnowska, Samanta Makurat, Magdalena Zdrowowicz, Janusz Rak
    Academic press:
    The Journal of Physical Chemistry B (rok: 2017, tom: 121, strony: 6139−6147), Wydawca: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/acs.jpcb.7b03633 - link to the publication
  5. Dominant Pathways of Adenosyl Radical-Induced DNA Damage Revealed by QM/MM Metadynamics
    Authors:
    Paweł Wityk, Miłosz Wieczór, Samanta Makurat, Lidia Chomicz-Mańka, Jacek Czub, Janusz Rak
    Academic press:
    Journal of Chemical Theory and Computations (rok: 2017, tom: 13, strony: 6415−6423), Wydawca: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/acs.jctc.7b00978 - link to the publication
  6. Electron-Induced Dissociation of the Potential Radiosensitizer 5‑Selenocyanato-2′-deoxyuridine
    Authors:
    Rebecca Meißner, Samanta Makurat, Witold Kozak, Paulo Limão-Vieira, Janusz Rak, Stephan Denifl
    Academic press:
    The Journal of Physical Chemistry B (rok: 2019, tom: 123, strony: 1274−1282), Wydawca: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/acs.jpcb.8b11523 - link to the publication
  7. Electrophilic 5-Substituted Uracils as Potential Radiosensitizers. A DFT Study
    Authors:
    Samanta Makurat, Lidia Chomicz-Mańka, Janusz Rak
    Academic press:
    ChemPhysChem (rok: 2016, tom: 17, strony: 2572-2578), Wydawca: WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
    Status:
    Published
    DOI:
    10.1002/cphc.201600240 - link to the publication
  8. Low-energy electron-induced decomposition of 5-trifluoromethanesulfonyl-uracil: A potential radiosensitizer
    Authors:
    J. Ameixa, E. Arthur-Baidoo, R. Meißner, S. Makurat, W. Kozak, K. Butowska, F. Ferreira da Silva, J. Rak, S. Denifl
    Academic press:
    The Journal of Chemical Physics (rok: 2018, tom: 149, strony: 164307), Wydawca: AIP Publishing
    Status:
    Published
    DOI:
    10.1063/1.5050594 - link to the publication
  9. 5-Selenocyanato and 5-trifluoromethanesulfonyl derivatives of 20-deoxyuridine: synthesis, radiation and computational chemistry as well as cytotoxicity
    Authors:
    Samanta Makurat, Magdalena Zdrowowicz, Lidia Chomicz-Mańka, Witold Kozak, Illia E. Serdiuk, Paweł Wityk, Alicja Kawecka, Marta Sosnowska, Janusz Rak
    Academic press:
    RSC Advances (rok: 2018, tom: 8, strony: 21378–21388), Wydawca: Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/C8RA03172J - link to the publication
  10. Modifications at the C(5) position of pyrimidine nucleosides
    Authors:
    Witold Kozak, Sebastian Demkowicz, Mateusz DasÂko, Janusz Rachon, Janusz Rak
    Academic press:
    Russian Chemical Reviews (rok: 2020, tom: 89, strony: 281-310), Wydawca: IOP Publishing
    Status:
    Published
    DOI:
    10.1070/RCR4919 - link to the publication
  11. Photoinduced electron transfer in 5-bromouracil labeled DNA. A contrathermodynamic mechanism revisited by electron transfer theories
    Authors:
    Lorenzo Cupellini, Paweł Wityk, Benedetta Mennucci, Janusz Rak
    Academic press:
    Physical Chemistry Chemical Physics (rok: 2019, tom: 21, strony: 4387-4393), Wydawca: The Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/c8cp07700b - link to the publication
  12. UV-induced electron transfer between triethylamine and 5-bromo-2'-deoxyuridine. A puzzle concerning the photochemical debromination of labelled DNA
    Authors:
    Paweł Wityk, Magdalena Zdrowowicz, Justyna Wiczk and Janusz Rak
    Academic press:
    Journal of Pharmaceutical and Biomedical Analysis (rok: 2017, tom: 142, strony: 262–269), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.jpba.2017.04.044 - link to the publication
  13. Uracil-5-yl O‑Sulfamate: An Illusive Radiosensitizer. Pitfalls in Modeling the Radiosensitizing Derivatives of Nucleobases
    Authors:
    Paulina Spisz, Magdalena Zdrowowicz, Witold Kozak, Lidia Chomicz-Mańka, Karina Falkiewicz, Samanta Makurat, Artur Sikorski, Dariusz Wyrzykowski, Janusz Rak, Eugene Arthur-Baidoo, Patrick Ziegler, Mateus Salomao Rodrigues Costa, and Stephan Denifl
    Academic press:
    The Journal of Physical Chemistry B (rok: 2020, tom: 124, strony: 5600−5613), Wydawca: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/acs.jpcb.0c03844 - link to the publication
  14. 5-Iodo-4-thio-20-Deoxyuridine as a Sensitizer of X-ray Induced Cancer Cell Killing
    Authors:
    Samanta Makurat, Paulina Spisz, Witold Kozak, Janusz Rak, Magdalena Zdrowowicz
    Academic press:
    International Journal o f Molecular Sciences (rok: 2019, tom: 20, strony: 1308), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms20061308 - link to the publication
  15. Electron-Induced Decomposition of Uracil-5-yl O-(N,N-dimethylsulfamate): Role of Methylation in Molecular Stability
    Authors:
    Eugene Arthur-Baidoo, Karina Falkiewicz, Lidia Chomicz-Mańka, Anna Czaja, Sebastian Demkowicz, Karol Biernacki, Witold Kozak, Janusz Rak, Stephan Denifl
    Academic press:
    International Journal of Molecular Sciences (rok: 2021, tom: 22, strony: 2344), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms22052344 - link to the publication
  16. Why Does the Type of Halogen Atom Matter for the Radiosensitizing Properties of 5-Halogen Substituted 4-Thio-20-Deoxyuridines?
    Authors:
    Paulina Spisz, Magdalena Zdrowowicz, Samanta Makurat, Witold Kozak, Konrad Skotnicki, Krzysztof Bobrowski, Janusz Rak
    Academic press:
    Molecules (rok: 2019, tom: 24, strony: 2819), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/molecules24152819 - link to the publication
  1. Consequences of Electron Attachment to Modified Nucleosides Incorporated into DNA
    Authors:
    Lidia Chomicz-Mańka, Paweł Wityk, Łukasz Golon, Magdalena Zdrowowicz, Justyna Wiczk, Kinga Westphal, Michał Żyndul, Samanta Makurat, Janusz Rak
    Book:
    Handbook of Computational Chemistry (rok: 2017, tom: brak, strony: 1895-1916), Wydawca: Springer
    Status:
    Published
  2. DNA damage radiosensitizers geared towards hydrated electrons
    Authors:
    Magdalena Zdrowowicz, Lidia Chomicz-Mańka, Kamila Butowska, Paulina Spisz, Karina Falkiewicz, Anna Czaja, Janusz Rak
    Book:
    Practical Aspects of Computational Chemistry-V , Wydawca: Springer
    Status:
    Accepted for publication

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