Projects funded by the NCN

Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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From a molecular in silico model to cellular response in vitro. Modified nucleosides as photo and/or radiosensitizers of DNA damage

2014/14/A/ST4/00405

Keywords:

modified nucleosides rdiosensitizers photosensitizers DNA double strand breaks cellular response anticancer therapy

Descriptors:

  • ST4_17: Chemical reactions: mechanisms, thermodynamics, kinetics
  • ST4_15: Colloid chemistry
  • ST4_16: Ionic liquids

Panel:

ST4 - Chemistry: physical chemistry/chemical physics, theoretical chemistry, analytical chemistry, inorganic chemistry, organic chemistry, method development

Host institution :

Uniwersytet Gdański, Wydział Chemii

woj. pomorskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Janusz Rak 

Number of co-investigators in the project: 21

Call: MAESTRO 6 - announced on 2014-06-16

Amount awarded: 3 958 291 PLN

Project start date (Y-m-d): 2015-04-23

Project end date (Y-m-d): 2021-01-22

Project duration:: 69 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Cytometr przepływowy (264 450 PLN)
  2. Komora do naświetlań UV (100 000 PLN)
  3. Niskotemperaturowa zamrażarka (26 000 PLN)
  4. Preparatywny system HPLC (251 000 PLN)
  5. System do analizy Western blot (12 500 PLN)
  6. Inkubator z kontrolą ciśnienia tlenu (37 000 PLN)
  7. Odwrócony mikroskop fluorescencyjny (122 000 PLN)
  8. System do liofilizacji z rozpuszczalników niewodnych.
  9. System elektroforetyczny do sekwencjonowania DNA (17 000 PLN)
  10. Aparat do pomiarów stężenia tlenu w kulturach komórkowych (158 000 PLN)
  11. Klaster komputerowy (350 000 PLN)
  12. Komora do napromieniań promieniowaniem X (320 000 PLN)
  13. System UHPLC (362 000 PLN)
  14. Wirówka z chłodzeniem (16 000 PLN)
  15. system do oczyszczania wody.

Information in the final report

  • Publication in academic press/journals (16)
  • Book publications / chapters in book publications (2)
  1. 5-Selenocyanatouracil: A Potential Hypoxic Radiosensitizer. Electron Attachment Induced Formation of Selenium Centered Radical
    Authors:
    Marta Sosnowska, Samanta Makurat, Magdalena Zdrowowicz, Janusz Rak
    Academic press:
    The Journal of Physical Chemistry B (rok: 2017, tom: 121, strony: 6139−6147), Wydawca: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/acs.jpcb.7b03633 - link to the publication
  2. Electron-Induced Dissociation of the Potential Radiosensitizer 5‑Selenocyanato-2′-deoxyuridine
    Authors:
    Rebecca Meißner, Samanta Makurat, Witold Kozak, Paulo Limão-Vieira, Janusz Rak, Stephan Denifl
    Academic press:
    The Journal of Physical Chemistry B (rok: 2019, tom: 123, strony: 1274−1282), Wydawca: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/acs.jpcb.8b11523 - link to the publication
  3. Molecular features of thymidine analogues governing the activity of human thymidine kinase
    Authors:
    Karolina Jagiello, Samanta Makurat, Sylwester Pereć, Janusz Rak, Tomasz Puzyn
    Academic press:
    Structural Chemistry (rok: 2018, tom: 29, strony: 1367–1374), Wydawca: Springer
    Status:
    Published
    DOI:
    10.1007/s11224-018-1124-2 - link to the publication
  4. UV-induced electron transfer between triethylamine and 5-bromo-2'-deoxyuridine. A puzzle concerning the photochemical debromination of labelled DNA
    Authors:
    Paweł Wityk, Magdalena Zdrowowicz, Justyna Wiczk and Janusz Rak
    Academic press:
    Journal of Pharmaceutical and Biomedical Analysis (rok: 2017, tom: 142, strony: 262–269), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.jpba.2017.04.044 - link to the publication
  5. 5-Iodo-4-thio-20-Deoxyuridine as a Sensitizer of X-ray Induced Cancer Cell Killing
    Authors:
    Samanta Makurat, Paulina Spisz, Witold Kozak, Janusz Rak, Magdalena Zdrowowicz
    Academic press:
    International Journal o f Molecular Sciences (rok: 2019, tom: 20, strony: 1308), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms20061308 - link to the publication
  6. 5-Selenocyanato and 5-trifluoromethanesulfonyl derivatives of 20-deoxyuridine: synthesis, radiation and computational chemistry as well as cytotoxicity
    Authors:
    Samanta Makurat, Magdalena Zdrowowicz, Lidia Chomicz-Mańka, Witold Kozak, Illia E. Serdiuk, Paweł Wityk, Alicja Kawecka, Marta Sosnowska, Janusz Rak
    Academic press:
    RSC Advances (rok: 2018, tom: 8, strony: 21378–21388), Wydawca: Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/C8RA03172J - link to the publication
  7. Electron-Induced Decomposition of Uracil-5-yl O-(N,N-dimethylsulfamate): Role of Methylation in Molecular Stability
    Authors:
    Eugene Arthur-Baidoo, Karina Falkiewicz, Lidia Chomicz-Mańka, Anna Czaja, Sebastian Demkowicz, Karol Biernacki, Witold Kozak, Janusz Rak, Stephan Denifl
    Academic press:
    International Journal of Molecular Sciences (rok: 2021, tom: 22, strony: 2344), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms22052344 - link to the publication
  8. Low-energy electron-induced decomposition of 5-trifluoromethanesulfonyl-uracil: A potential radiosensitizer
    Authors:
    J. Ameixa, E. Arthur-Baidoo, R. Meißner, S. Makurat, W. Kozak, K. Butowska, F. Ferreira da Silva, J. Rak, S. Denifl
    Academic press:
    The Journal of Chemical Physics (rok: 2018, tom: 149, strony: 164307), Wydawca: AIP Publishing
    Status:
    Published
    DOI:
    10.1063/1.5050594 - link to the publication
  9. 5-(N-Trifluoromethylcarboxy)aminouracil as a Potential DNA Radiosensitizer and Its Radiochemical Conversion into N-Uracil-5-yloxamic Acid
    Authors:
    Paulina Spisz, Witold Kozak, Lidia Chomicz-Mańka, Samanta Makurat, Karina Falkiewicz, Artur Sikorski, Anna Czaja, Janusz Rak, Magdalena Zdrowowicz
    Academic press:
    International Journal o f Molecular Sciences (rok: 2020, tom: 21, strony: 6352), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/ijms21176352 - link to the publication
  10. Dominant Pathways of Adenosyl Radical-Induced DNA Damage Revealed by QM/MM Metadynamics
    Authors:
    Paweł Wityk, Miłosz Wieczór, Samanta Makurat, Lidia Chomicz-Mańka, Jacek Czub, Janusz Rak
    Academic press:
    Journal of Chemical Theory and Computations (rok: 2017, tom: 13, strony: 6415−6423), Wydawca: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/acs.jctc.7b00978 - link to the publication
  11. Why Does the Type of Halogen Atom Matter for the Radiosensitizing Properties of 5-Halogen Substituted 4-Thio-20-Deoxyuridines?
    Authors:
    Paulina Spisz, Magdalena Zdrowowicz, Samanta Makurat, Witold Kozak, Konrad Skotnicki, Krzysztof Bobrowski, Janusz Rak
    Academic press:
    Molecules (rok: 2019, tom: 24, strony: 2819), Wydawca: MDPI
    Status:
    Published
    DOI:
    10.3390/molecules24152819 - link to the publication
  12. Photoinduced electron transfer in 5-bromouracil labeled DNA. A contrathermodynamic mechanism revisited by electron transfer theories
    Authors:
    Lorenzo Cupellini, Paweł Wityk, Benedetta Mennucci, Janusz Rak
    Academic press:
    Physical Chemistry Chemical Physics (rok: 2019, tom: 21, strony: 4387-4393), Wydawca: The Royal Society of Chemistry
    Status:
    Published
    DOI:
    10.1039/c8cp07700b - link to the publication
  13. Electrophilic 5-Substituted Uracils as Potential Radiosensitizers. A DFT Study
    Authors:
    Samanta Makurat, Lidia Chomicz-Mańka, Janusz Rak
    Academic press:
    ChemPhysChem (rok: 2016, tom: 17, strony: 2572-2578), Wydawca: WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
    Status:
    Published
    DOI:
    10.1002/cphc.201600240 - link to the publication
  14. Modifications at the C(5) position of pyrimidine nucleosides
    Authors:
    Witold Kozak, Sebastian Demkowicz, Mateusz DasÂko, Janusz Rachon, Janusz Rak
    Academic press:
    Russian Chemical Reviews (rok: 2020, tom: 89, strony: 281-310), Wydawca: IOP Publishing
    Status:
    Published
    DOI:
    10.1070/RCR4919 - link to the publication
  15. The Sequence Dependence of Photoinduced Single Strand Break in 5-Bromo-2′-deoxyuridine Labeled DNA Supports That Electron Transfer Is Responsible for the Damage
    Authors:
    Kinga Westphal, Samanta Makurat, Janusz Rak
    Academic press:
    The Journal of Physical Chemistry B (rok: 2017, tom: 121, strony: 9169−9174), Wydawca: American Chemical Socitey
    Status:
    Published
    DOI:
    10.1021/acs.jpcb.7b07338 - link to the publication
  16. Uracil-5-yl O‑Sulfamate: An Illusive Radiosensitizer. Pitfalls in Modeling the Radiosensitizing Derivatives of Nucleobases
    Authors:
    Paulina Spisz, Magdalena Zdrowowicz, Witold Kozak, Lidia Chomicz-Mańka, Karina Falkiewicz, Samanta Makurat, Artur Sikorski, Dariusz Wyrzykowski, Janusz Rak, Eugene Arthur-Baidoo, Patrick Ziegler, Mateus Salomao Rodrigues Costa, and Stephan Denifl
    Academic press:
    The Journal of Physical Chemistry B (rok: 2020, tom: 124, strony: 5600−5613), Wydawca: American Chemical Society
    Status:
    Published
    DOI:
    10.1021/acs.jpcb.0c03844 - link to the publication
  1. Consequences of Electron Attachment to Modified Nucleosides Incorporated into DNA
    Authors:
    Lidia Chomicz-Mańka, Paweł Wityk, Łukasz Golon, Magdalena Zdrowowicz, Justyna Wiczk, Kinga Westphal, Michał Żyndul, Samanta Makurat, Janusz Rak
    Book:
    Handbook of Computational Chemistry (rok: 2017, tom: brak, strony: 1895-1916), Wydawca: Springer
    Status:
    Published
  2. DNA damage radiosensitizers geared towards hydrated electrons
    Authors:
    Magdalena Zdrowowicz, Lidia Chomicz-Mańka, Kamila Butowska, Paulina Spisz, Karina Falkiewicz, Anna Czaja, Janusz Rak
    Book:
    Practical Aspects of Computational Chemistry-V , Wydawca: Springer
    Status:
    Accepted for publication

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