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Characterization of a new essential TPR protein LapB and a new non-coding RNA, which together regulate the assembly of endotoxin

2013/11/B/NZ2/02641

Keywords

Descriptors

  • NZ2_1: Genetyka molekularna
  • NZ2_2: Genomika, transkryptomika i epigenomika
  • NZ1_10: Biologia drobnoustrojów

Panel

NZ2 – Genetics, genomics: molecular genetics, genomics, proteomics, bioinformatics, systems biology, genetic epidemiology

Host institution

Politechnika Gdańska, Wydział Chemiczny

Voivodeship: pomorskie

Other projects carried out by the institution »

Principal investigator (from the host institution)

prof. Satish Raina

Number of co-investigators in the project: 5

Call

OPUS 6 – announced on 2013-09-16

Amount awarded
746 960 PLN
Project start date
2014-07-09
Project end date
2017-07-08
Project duration
36 months (the same as in the proposal)
Project status
Project settled

Equipment purchased [PL]

  1. Mikrowirówka (table-top centrifuge).
  2. System do elektroforezy dwukierunkowej 2D-PAGE IEF. Cost: 55 000 PLN
  3. Wirówka szybkoobrotowa z chłodzeniem, z rotorem kątowym, adapterami i naczyniami wirowniczymi. Cost: 147 000 PLN
  4. Zamrażarka -80°C ze statywami.
  5. Zamrażarka laboratoryjna -20°C.

Information in the final report

Publication in academic press/journals (3)

  1. Small regulatory bacterial RNAs regulating the envelope stress response

    Authors:
    G. Klein and S. Raina
    Academic press:
    Biochemical Society Transactions (year: 2017, volume: 45, pages: 417-425), Publisher: Portland Press Biochemical Society
    Status:
    Published
    DOI:
    10.1042/BST20160367 – link to the publication

  2. Multiple transcriptional factors regulate transcription of the rpoE gene in Escherichia coli under different growth conditions and when the lipopolysaccharide biosynthesis is defective

    Authors:
    Gracjana Klein, Anna Stupak, Daria Biernacka, Pawel Wojtkiewicz, Buko Lindner and Satish Raina
    Academic press:
    The Journal of Biological Chemistry (year: 2016, volume: 291, pages: 22999-23019), Publisher: American Society of Biochemistry and Molecular Biology
    Status:
    Published
    DOI:
    10.1074/jbc.M116.748954 – link to the publication

  3. Regulated Control of the Assembly and Diversity of LPS by Noncoding sRNAs

    Authors:
    Gracjana Klein and Satish Raina
    Academic press:
    BioMed Research International (year: 2015, volume: 2015, pages: 46038), Publisher: Hindawi Publication Corporation
    Status:
    Published
    DOI:
    10.1155/2015/153561 – link to the publication

Articles in post-conference publications (8)

  1. Regulation of LPS biosynthesis and sensing of LPS defects by the RpoE sigma factor

    Authors:
    Gracjana Klein, Anna Stupak, Daria Biernacka, Pawel Wojtkiewicz, Buko Lindner and Satish Raina
    Conference:
    14th Biennial Meeting of the International Endotoxin Innate Immunity Society (year: 2016, pages: 26), Publisher: International Endotoxin Innate Immunity Society
    Date:
    conference 22-24 September
    Status:
    Published

  2. Novel controls of balanced LPS biosynthesis by LapB, non-coding RNAs and stress responsive envelope biogenesis factors

    Authors:
    Satish Raina, Anna Stupak and Gracjana Klein
    Conference:
    14th Biennial Meeting of the International Endotoxin Innate Immunity Society (year: 2016, pages: 3), Publisher: International Endotoxin Innate Immunity Society
    Date:
    conference 22-24 September
    Status:
    Published

  3. Regulation of LPS composition, biosynthesis and critical checkpoints in its assembly by non-coding regulatory RNAs

    Authors:
    S. Raina* and G. Klein
    Conference:
    Microbiology and Infections 2017 (year: 2017, pages: 219), Publisher: Springer
    Date:
    conference 05.03.2017--08.03.2017
    Status:
    Published

  4. Integrated network of different sigma factors, two-component systems and non-coding RNAs that regulate transcription of the rpoE gene encoding ECF sigma factor in Escherichia coli in response to different signals

    Authors:
    G. Klein*, A. Stupak, P. Wojtkiewicz, D. Biernacka and S. Raina
    Conference:
    Microbiology and Infections 2017 (year: 2017, pages: 218), Publisher: Springer
    Date:
    conference 05.03.2017-08.03.2017
    Status:
    Published

  5. Regulated assembly of LPS: mechanism of coupling of LPS synthesis and its translocation

    Authors:
    Satish Raina, Gracjana Klein
    Conference:
    The 6th Baltic Meeting on Microbial Carbohydrates (year: 2014, pages: 21), Publisher: Pro Science Polska, Fundacja Centrum Transferu Technologii Synergy
    Date:
    conference 7-10 September
    Status:
    Published

  6. Assembly of LPs in Escherichia coli requires the essential LpaB heat shock protein and its modulation by a new sRNA slrA

    Authors:
    Gracjana Klein, Satish Raina
    Conference:
    The 6th Baltic Meeting on Microbial Carbohydrates (year: 2014, pages: 71), Publisher: Pro Science Polska, Fundacja Centrum Transferu Technologii Synergy
    Date:
    conference 7-10 September
    Status:
    Published

  7. Regulated assembly and diversity of lipopolysaccharide by non-coding sRNAs

    Authors:
    Gracjana Klein, Anna Stupak, Daria Biernacka, Pawel Wojtkiewicz and Satish Raina
    Conference:
    81st Harden Conference: RNA and Disease,https://www.biochemistry.org/Events/tabid/379/View/Programme/Filter/64/MeetingNo/81HDN/Default.aspx (year: 2016, pages: p14), Publisher: Biochemical Society , London
    Date:
    conference 4-7 September
    Status:
    Published

  8. Coordinated assembly of LPS by TPR-containing LapB heat shock protein along with DnaKJ chaperones

    Authors:
    Satish Raina, Anna Stupak, Patrycja Gorzelak, Gracjana Klein
    Conference:
    The Biology of Molecular Chaperones, EMBO Conference (year: 2015, pages: p152), Publisher: EMBO
    Date:
    conference 8-13 May
    Status:
    Published

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