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Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Characterization of a new essential TPR protein LapB and a new non-coding RNA, which together regulate the assembly of endotoxin

2013/11/B/NZ2/02641

Keywords:

tetratricopeptide repeat (TPR) proteins lipopolisaccharide (LPS) endotoxin lipid A non-coding RNA CHIR-090 Lpt transport proteins Kdo transferase

Descriptors:

  • NZ2_1: Molecular genetics
  • NZ2_2: Genomics, transcriptomics, epigenomics
  • NZ1_10: Microbiology

Panel:

NZ2 - Genetics, genomics: molecular genetics, genomics, proteomics, bioinformatics, systems biology, genetic epidemiology

Host institution :

Politechnika Gdańska, Wydział Chemiczny

woj. pomorskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Satish Raina 

Number of co-investigators in the project: 5

Call: OPUS 6 - announced on 2013-09-16

Amount awarded: 746 960 PLN

Project start date (Y-m-d): 2014-07-09

Project end date (Y-m-d): 2017-07-08

Project duration:: 36 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. System do elektroforezy dwukierunkowej 2D-PAGE IEF (55 000 PLN)
  2. Mikrowirówka (table-top centrifuge).
  3. Wirówka szybkoobrotowa z chłodzeniem, z rotorem kątowym, adapterami i naczyniami wirowniczymi (147 000 PLN)
  4. Zamrażarka -80°C ze statywami.
  5. Zamrażarka laboratoryjna -20°C.

Information in the final report

  • Publication in academic press/journals (4)
  • Articles in post-conference publications (8)
  1. Multiple transcriptional factors regulate transcription of the rpoE gene in Escherichia coli under different growth conditions and when the lipopolysaccharide biosynthesis is defective
    Authors:
    Gracjana Klein, Anna Stupak, Daria Biernacka, Pawel Wojtkiewicz, Buko Lindner and Satish Raina
    Academic press:
    The Journal of Biological Chemistry (rok: 2016, tom: 291, strony: 22999-23019), Wydawca: American Society of Biochemistry and Molecular Biology
    Status:
    Published
    DOI:
    10.1074/jbc.M116.748954 - link to the publication
  2. Small regulatory bacterial RNAs regulating the envelope stress response
    Authors:
    G. Klein and S. Raina
    Academic press:
    Biochemical Society Transactions (rok: 2017, tom: 45, strony: 417-425), Wydawca: Portland Press Biochemical Society
    Status:
    Published
    DOI:
    10.1042/BST20160367 - link to the publication
  3. Regulated Control of the Assembly and Diversity of LPS by Noncoding sRNAs
    Authors:
    Gracjana Klein and Satish Raina
    Academic press:
    BioMed Research International (rok: 2015, tom: 2015, strony: 45307), Wydawca: Hindawi Publication Corporation
    Status:
    Published
    DOI:
    10.1155/2015/153561 - link to the publication
  4. Regulated Control of the Assembly and Diversity of LPS by Noncoding sRNAs
    Authors:
    Gracjana Klein and Satish Raina
    Academic press:
    BioMed Research International (rok: 2015, tom: 2015, strony: 45307), Wydawca: Hindawi Publication Corporation
    Status:
    Published
    DOI:
    10.1155/2015/153561 - link to the publication
  1. Novel controls of balanced LPS biosynthesis by LapB, non-coding RNAs and stress responsive envelope biogenesis factors
    Authors:
    Satish Raina, Anna Stupak and Gracjana Klein
    Conference:
    14th Biennial Meeting of the International Endotoxin Innate Immunity Society (rok: 2016, ), Wydawca: International Endotoxin Innate Immunity Society
    Data:
    konferencja 22-24 September
    Status:
    Published
  2. Regulated assembly of LPS: mechanism of coupling of LPS synthesis and its translocation
    Authors:
    Satish Raina, Gracjana Klein
    Conference:
    The 6th Baltic Meeting on Microbial Carbohydrates (rok: 2014, ), Wydawca: Pro Science Polska, Fundacja Centrum Transferu Technologii Synergy
    Data:
    konferencja 7-10 September
    Status:
    Published
  3. Regulation of LPS composition, biosynthesis and critical checkpoints in its assembly by non-coding regulatory RNAs
    Authors:
    S. Raina* and G. Klein
    Conference:
    Microbiology and Infections 2017 (rok: 2017, ), Wydawca: Springer
    Data:
    konferencja 05.03.2017--08.03.2017
    Status:
    Published
  4. Coordinated assembly of LPS by TPR-containing LapB heat shock protein along with DnaKJ chaperones
    Authors:
    Satish Raina, Anna Stupak, Patrycja Gorzelak, Gracjana Klein
    Conference:
    The Biology of Molecular Chaperones, EMBO Conference (rok: 2015, ), Wydawca: EMBO
    Data:
    konferencja 8-13 May
    Status:
    Published
  5. Integrated network of different sigma factors, two-component systems and non-coding RNAs that regulate transcription of the rpoE gene encoding ECF sigma factor in Escherichia coli in response to different signals
    Authors:
    G. Klein*, A. Stupak, P. Wojtkiewicz, D. Biernacka and S. Raina
    Conference:
    Microbiology and Infections 2017 (rok: 2017, ), Wydawca: Springer
    Data:
    konferencja 05.03.2017-08.03.2017
    Status:
    Published
  6. Assembly of LPs in Escherichia coli requires the essential LpaB heat shock protein and its modulation by a new sRNA slrA
    Authors:
    Gracjana Klein, Satish Raina
    Conference:
    The 6th Baltic Meeting on Microbial Carbohydrates (rok: 2014, ), Wydawca: Pro Science Polska, Fundacja Centrum Transferu Technologii Synergy
    Data:
    konferencja 7-10 September
    Status:
    Published
  7. Regulated assembly and diversity of lipopolysaccharide by non-coding sRNAs
    Authors:
    Gracjana Klein, Anna Stupak, Daria Biernacka, Pawel Wojtkiewicz and Satish Raina
    Conference:
    81st Harden Conference: RNA and Disease,https://www.biochemistry.org/Events/tabid/379/View/Programme/Filter/64/MeetingNo/81HDN/Default.aspx (rok: 2016, ), Wydawca: Biochemical Society , London
    Data:
    konferencja 4-7 September
    Status:
    Published
  8. Regulation of LPS biosynthesis and sensing of LPS defects by the RpoE sigma factor
    Authors:
    Gracjana Klein, Anna Stupak, Daria Biernacka, Pawel Wojtkiewicz, Buko Lindner and Satish Raina
    Conference:
    14th Biennial Meeting of the International Endotoxin Innate Immunity Society (rok: 2016, ), Wydawca: International Endotoxin Innate Immunity Society
    Data:
    konferencja 22-24 September
    Status:
    Published

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